bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-36_CDS_annotation_glimmer3.pl_2_4
Length=228
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_A_034_Microviridae_AG0105_putative.VP1 451 4e-158
Alpavirinae_Human_gut_33_005_Microviridae_AG0184_putative.VP1 375 1e-128
Alpavirinae_Human_feces_A_021_Microviridae_AG077_putative.VP1 192 8e-59
Alpavirinae_Human_feces_D_015_Microviridae_AG019_putative.VP1 181 1e-54
Alpavirinae_Human_feces_C_043_Microviridae_AG0322_putative.VP1 179 6e-54
Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1 167 1e-49
Alpavirinae_Human_feces_D_008_Microviridae_AG098_putative.VP1 165 8e-49
Alpavirinae_Human_feces_D_031_Microviridae_AG0425_putative.VP1 165 9e-49
Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1 159 1e-46
Alpavirinae_Human_feces_E_011_Microviridae_AG0388_putative.VP1 150 1e-43
> Alpavirinae_Human_feces_A_034_Microviridae_AG0105_putative.VP1
Length=618
Score = 451 bits (1159), Expect = 4e-158, Method: Compositional matrix adjust.
Identities = 209/228 (92%), Positives = 219/228 (96%), Gaps = 0/228 (0%)
Query 1 MSSLFSYGDIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQP 60
MSSLFSYGDIKN+PRRSGFDLSNKCAFTAKVGELLPVYWKFCLP DKFNISQEWF RTQP
Sbjct 1 MSSLFSYGDIKNSPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPGDKFNISQEWFTRTQP 60
Query 61 VDTSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASSSASISFNRNLPWIDLA 120
VDTSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAAS S+SISFNRNLPW+DL+
Sbjct 61 VDTSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASGSSSISFNRNLPWVDLS 120
Query 121 TINTAIGNVQSSASPNNFFGVLRSEGFKKLVSYLGYGETSPEKYVDNLRCSALPLYAYQK 180
TIN AIGNVQSS SP NFFGV RSEGFKKLVSYLGYGETSPEKYVDNLRCSA PLYAYQK
Sbjct 121 TINVAIGNVQSSTSPKNFFGVSRSEGFKKLVSYLGYGETSPEKYVDNLRCSAFPLYAYQK 180
Query 181 IYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPVASSLDLFSQNPNDSVF 228
IYQDYYRHSQWEKSKPWTYNCDFWNGEDSTP+ ++++LFSQ+PNDSVF
Sbjct 181 IYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPITATVELFSQSPNDSVF 228
> Alpavirinae_Human_gut_33_005_Microviridae_AG0184_putative.VP1
Length=618
Score = 375 bits (963), Expect = 1e-128, Method: Compositional matrix adjust.
Identities = 176/228 (77%), Positives = 191/228 (84%), Gaps = 0/228 (0%)
Query 1 MSSLFSYGDIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQP 60
M+ LFSYGDIKN PRRSGFDL NK AFTAKVGELLPVYWKFC+P DKF+ISQEWF RTQP
Sbjct 1 MAGLFSYGDIKNKPRRSGFDLGNKNAFTAKVGELLPVYWKFCMPGDKFHISQEWFTRTQP 60
Query 61 VDTSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASSSASISFNRNLPWIDLA 120
VDTSAFTRIREYYEWFFVPLHL+YRNSNEAIMS+ENQPNYAAS + SI FNR LPW+DL
Sbjct 61 VDTSAFTRIREYYEWFFVPLHLMYRNSNEAIMSLENQPNYAASGTQSIVFNRKLPWVDLQ 120
Query 121 TINTAIGNVQSSASPNNFFGVLRSEGFKKLVSYLGYGETSPEKYVDNLRCSALPLYAYQK 180
T+N AI NVQ+S NN FG RS GF KL + LG GET P K + NLR SA P YAYQK
Sbjct 121 TLNDAITNVQASTYHNNMFGFARSSGFYKLFNSLGVGETDPSKTLANLRISAFPFYAYQK 180
Query 181 IYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPVASSLDLFSQNPNDSVF 228
IY D+YR+SQWE +KPWTYNCDFWNGEDSTPVA + DLF NPNDSVF
Sbjct 181 IYSDHYRNSQWEVNKPWTYNCDFWNGEDSTPVAFTKDLFDTNPNDSVF 228
> Alpavirinae_Human_feces_A_021_Microviridae_AG077_putative.VP1
Length=622
Score = 192 bits (489), Expect = 8e-59, Method: Compositional matrix adjust.
Identities = 101/240 (42%), Positives = 141/240 (59%), Gaps = 26/240 (11%)
Query 3 SLFSYGDIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQPVD 62
LFSY D+KN PRRSGFDL+ + FT+K GELLPVYWK +P DK+NIS WF RTQPVD
Sbjct 2 GLFSYDDLKNHPRRSGFDLTKRICFTSKAGELLPVYWKPTIPGDKWNISTNWFTRTQPVD 61
Query 63 TSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASSSASISFNRNLPWIDLATI 122
T+A+TR++EY +WFFVPL+L+ + AI M + P A S+ + + ++P+ L ++
Sbjct 62 TAAYTRVKEYVDWFFVPLNLIQKGIESAITQMVDNPVSAMSAIENRAITTDMPYTTLLSL 121
Query 123 NTAI------GNVQSSASPNNFFGVLRSEGFKKLVSYLGYGE-TSPEK------------ 163
+ A+ V S A N FG R++ KL+ L YG +PE
Sbjct 122 SRALYMLNGKSYVNSHAGKLNMFGFSRADLSAKLLQMLKYGNFINPEHSGLDTPMFGYST 181
Query 164 -------YVDNLRCSALPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPVASSL 216
Y+ N + LP++ YQKIY DY+R QWEK P+TYN D++ G D P +++
Sbjct 182 VKLAQFSYLWNQAVNVLPIFCYQKIYSDYFRFQQWEKPVPYTYNADYYAGGDIFPNPTTM 241
> Alpavirinae_Human_feces_D_015_Microviridae_AG019_putative.VP1
Length=584
Score = 181 bits (458), Expect = 1e-54, Method: Compositional matrix adjust.
Identities = 93/232 (40%), Positives = 139/232 (60%), Gaps = 25/232 (11%)
Query 3 SLFSYGDIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQPVD 62
LF+ +KN PRRSGFDLS+K AF+AKVGELLP+ W +P DKF++ ++ F RTQPV+
Sbjct 2 GLFNLSSVKNRPRRSGFDLSSKVAFSAKVGELLPIKWTLTMPGDKFSLKEQHFTRTQPVN 61
Query 63 TSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASSSASISFNRNLPWIDLATI 122
TSA+TR+REYY+WF+ PLHLL+RN+ E I ++ +A+S S+ N+P I
Sbjct 62 TSAYTRVREYYDWFWCPLHLLWRNAPEVIAQIQQNVQHASSFDGSVLLGSNMPCFSADQI 121
Query 123 NTAIGNVQSSASPNNFFGVLRSEGFKKLVSYL-------GYGETSPEKY---VD------ 166
+ ++ ++S N+FG R++ KL+ YL G G + Y VD
Sbjct 122 SQSLDMMKSKL---NYFGFNRADLAYKLIQYLRYGNVRTGVGTSGSRNYGTSVDVKDSSY 178
Query 167 ------NLRCSALPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPV 212
N S P+ AY+K QDY+R +QW+ S P+ +N D+++G+ +T +
Sbjct 179 NQNRAFNHALSVFPILAYKKFCQDYFRLTQWQVSAPYLWNIDYYDGKGATTI 230
> Alpavirinae_Human_feces_C_043_Microviridae_AG0322_putative.VP1
Length=602
Score = 179 bits (454), Expect = 6e-54, Method: Compositional matrix adjust.
Identities = 98/230 (43%), Positives = 138/230 (60%), Gaps = 25/230 (11%)
Query 1 MSSLFSYGDIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQP 60
M++LFSYG +KN P RSGFD S +FTAK GELLPVYWK LP K N++ F RT P
Sbjct 1 MANLFSYGSVKNKPARSGFDWSEHFSFTAKAGELLPVYWKMLLPGTKVNLNLSSFTRTMP 60
Query 61 VDTSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASSSASISFNRNLPWIDLA 120
V+T+A+TR++EYY+W+FVPL L+ ++ +A++ M++QP A S A+ S +LPW + A
Sbjct 61 VNTAAYTRVKEYYDWYFVPLRLINKSIGQALVQMQDQPVQATSIVANKSVTLDLPWTNAA 120
Query 121 TINTAI--GNVQSSASPNNFFGVLRSEGFKKLVSYLGYGE----TSPEKY---------- 164
T+ T + NV + + N G ++ KL+ YL YG + P K
Sbjct 121 TMFTLLNYANVILT-NKYNLDGFSKAATSAKLLRYLRYGNCYYTSDPSKVGKNKNFGLSS 179
Query 165 --------VDNLRCSALPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNG 206
N+ + LPL AYQKIY D++R QWE + P+TYN D++NG
Sbjct 180 KDDFNLLAAKNVSFNVLPLAAYQKIYCDWFRFEQWENACPYTYNFDYYNG 229
> Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1
Length=614
Score = 167 bits (423), Expect = 1e-49, Method: Compositional matrix adjust.
Identities = 94/237 (40%), Positives = 140/237 (59%), Gaps = 32/237 (14%)
Query 9 DIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQPVDTSAFTR 68
+++N P RSGFD+ K AFTAKVGELLPVYW +P DK+ + E+F RTQPV TSA+TR
Sbjct 9 NLQNHPHRSGFDIGRKNAFTAKVGELLPVYWDISMPGDKYRFNVEYFTRTQPVATSAYTR 68
Query 69 IREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAA-SSSASISFNRNLPWIDLATINTAI- 126
+REY++++ VPL LL++++ + M++ A S + ++S LP + + T+ AI
Sbjct 69 LREYFDFYAVPLRLLWKSAPSVLTQMQDVNQIQALSLTQNLSLGTYLPSLTIGTLGWAIR 128
Query 127 ---GNV---QSSASPNNFFGVLRSEGFKKLVSYLGYG---ETSP---------------- 161
GN ++++ N FG R++ KL+SYLGYG ET P
Sbjct 129 YLNGNTWEPETASYLRNAFGFSRADLSFKLLSYLGYGNLIETPPSLGNRWWSTSLKNTDD 188
Query 162 -----EKYVDNLRCSALPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPVA 213
++Y+ N + PL YQKIYQD++R QWEKS P +YN D+++G + V+
Sbjct 189 GANYTQQYIQNTIVNIFPLLTYQKIYQDFFRWPQWEKSNPSSYNVDYFSGSSPSIVS 245
> Alpavirinae_Human_feces_D_008_Microviridae_AG098_putative.VP1
Length=618
Score = 165 bits (417), Expect = 8e-49, Method: Compositional matrix adjust.
Identities = 92/235 (39%), Positives = 132/235 (56%), Gaps = 31/235 (13%)
Query 3 SLFSYGDIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQPVD 62
SLF+ +KN PRRSGFDLSN+ FT+K GELLPV+W P D F I + F RTQP++
Sbjct 2 SLFNMSAVKNHPRRSGFDLSNRVCFTSKAGELLPVFWDIVYPGDSFKIKTQLFTRTQPLN 61
Query 63 TSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASSSASISFNRNLPWIDLATI 122
T+A+TRIREY +++FVPL L+ +N A+ M++ P A S++ ++PW+ + +
Sbjct 62 TAAYTRIREYLDFYFVPLRLINKNLPTALTQMQDNPVQATGLSSNKVVTTDIPWVPVHSS 121
Query 123 N------TAIGNVQSSASPN---NFFGVLRSEGFKKLVSYLGYGETSPEKYVD------- 166
+ T +V+ S S + NF G KL+ YL YG D
Sbjct 122 SGSYSSLTGFADVRGSVSSSDIENFLGFDSITQSAKLLMYLRYGNFLSSVVPDEQKSIGL 181
Query 167 ----NLRCS-----------ALPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNG 206
+LR S LPL YQKIY D++R +QWEK++P+TYN D+++G
Sbjct 182 SSSLDLRNSETVSTGYTSVHILPLATYQKIYADFFRFTQWEKNQPYTYNFDWYSG 236
> Alpavirinae_Human_feces_D_031_Microviridae_AG0425_putative.VP1
Length=614
Score = 165 bits (417), Expect = 9e-49, Method: Compositional matrix adjust.
Identities = 94/242 (39%), Positives = 137/242 (57%), Gaps = 34/242 (14%)
Query 10 IKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQPVDTSAFTRI 69
+KN PRRSGFDLSN+ FT+K GELLPV+W P D F I + F RTQP++T+A+TRI
Sbjct 4 VKNHPRRSGFDLSNRVCFTSKAGELLPVFWDIVYPGDSFKIKTQLFTRTQPLNTAAYTRI 63
Query 70 REYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASSSASISFNRNLPWIDLATIN-----T 124
REY +++FVPL L+ +N A+M M++ P A S++ ++PW+ T
Sbjct 64 REYLDFYFVPLRLINKNLPTALMQMQDNPVQATGLSSNKVVTTDIPWVPTNLSGTYGSLT 123
Query 125 AIGNVQSSASPNNFFGVLRSEGFK------KLVSYLGYGETSPEKYVD-----------N 167
A+ +V++S P++ G+ GF KL+ YL YG D +
Sbjct 124 ALADVKNSF-PSSSTGIEDLLGFDAITQSAKLLMYLRYGNFLSSVVSDKNKSLGLSGSLD 182
Query 168 LRCS-----------ALPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPVASSL 216
LR S LPL AYQK Y D++R +QWEK++P+TYN D+++G + ++L
Sbjct 183 LRNSETASTGYTSMHILPLAAYQKAYADFFRFTQWEKNQPYTYNFDWYSGGNVLASLTTL 242
Query 217 DL 218
DL
Sbjct 243 DL 244
> Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1
Length=607
Score = 159 bits (401), Expect = 1e-46, Method: Compositional matrix adjust.
Identities = 91/235 (39%), Positives = 133/235 (57%), Gaps = 29/235 (12%)
Query 9 DIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQPVDTSAFTR 68
+++N P RSGFD+ K AFTAKVGELLPVYW +P DK+ + E+F RTQPV+TSA+TR
Sbjct 3 NLQNHPHRSGFDIGRKNAFTAKVGELLPVYWDISMPGDKYKFNVEYFTRTQPVETSAYTR 62
Query 69 IREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASS-SASISFNRNLPWIDL----ATIN 123
+REY++++ VPL LL++++ + M++ A S + ++S P + L A +N
Sbjct 63 LREYFDFYAVPLRLLWKSAPSVLTQMQDINQLQALSFTQNLSLGSYFPSLTLSRFTAVLN 122
Query 124 TAIGNVQSSASPNNF---FGVLRSEGFKKLVSYLGYGE---------------------T 159
G + + F FG R++ KL SYLGYG +
Sbjct 123 RLNGGSNVPGNSSTFLNEFGFSRADLAFKLFSYLGYGNVWSSEFSSSNRWWSTSLKGGGS 182
Query 160 SPEKYVDNLRCSALPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNGEDSTPVAS 214
++YV + + PL AYQKIYQD++R SQWE S P +YN D++ G V+S
Sbjct 183 YTQQYVQDSYVNLFPLLAYQKIYQDFFRWSQWENSNPSSYNVDYFTGVSPLLVSS 237
> Alpavirinae_Human_feces_E_011_Microviridae_AG0388_putative.VP1
Length=622
Score = 150 bits (379), Expect = 1e-43, Method: Compositional matrix adjust.
Identities = 84/245 (34%), Positives = 134/245 (55%), Gaps = 40/245 (16%)
Query 1 MSSLFSYGDIKNTPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPADKFNISQEWFARTQP 60
M+ +++N P ++GFD+ +K FTAKVGEL+PVYW F +P ++I +F RT+P
Sbjct 1 MAHFTGLKELQNHPHKAGFDVGSKNLFTAKVGELIPVYWDFAIPDCDYDIDLAYFTRTRP 60
Query 61 VDTSAFTRIREYYEWFFVPLHLLYRNSNEAIMSM-ENQPNYAASSSASISFNRNLPWIDL 119
V T+A+TR+REY++++ VP LL+++ + A++ M + P + + ++ ++PW L
Sbjct 61 VQTAAYTRVREYFDFYAVPCDLLWKSFDSAVIQMGQTAPVQSKTLLDPLTVGNDIPWCTL 120
Query 120 ATINTAIGNVQSSASP-----------NNFFGVLRSEGFKKLVSYLGYGE---------T 159
++ A+ S +SP N FG R + KL+ YL YG
Sbjct 121 LDLSNAV-YFSSGSSPLGSTVSVPSGFGNIFGYNRGDVDSKLLFYLNYGNFVNPSLSNVG 179
Query 160 SP------------------EKYVDNLRCSALPLYAYQKIYQDYYRHSQWEKSKPWTYNC 201
SP +KY++N + PL AYQKIYQD++R SQWE + P +YN
Sbjct 180 SPSNRWWNTSFSSSKVPGYSQKYLNNNAVNIFPLLAYQKIYQDFFRWSQWENADPTSYNV 239
Query 202 DFWNG 206
D++NG
Sbjct 240 DYYNG 244
Lambda K H a alpha
0.318 0.132 0.419 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 17737542