bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-43_CDS_annotation_glimmer3.pl_2_1
Length=306
Score E
Sequences producing significant alignments: (Bits) Value
Gokush_Human_gut_33_003_Microviridae_AG061_putative.VP1 500 3e-177
Gokush_Human_feces_E_007_Microviridae_AG0412_putative.VP1 468 5e-164
Gokush_Human_feces_E_017_Microviridae_AG0127_putative.VP1 360 2e-122
Gokush_Human_feces_A_020_Microviridae_AG0265_putative.VP1 358 2e-121
Gokush_Human_feces_A_019_Microviridae_AG0438_putative.VP1 355 2e-120
Gokush_Human_feces_B_029_Microviridae_AG0417_putative.VP1 290 2e-95
Gokush_Human_feces_B_068_Microviridae_AG0331_putative.VP1 281 1e-91
Gokush_Human_feces_E_009_Microviridae_AG0285_putative.VP1 276 1e-89
Gokush_Human_feces_A_029_Microviridae_AG0226_putative.VP1 272 3e-89
Gokush_Human_feces_A_013_Microviridae_AG009_putative.VP1 272 6e-88
> Gokush_Human_gut_33_003_Microviridae_AG061_putative.VP1
Length=545
Score = 500 bits (1288), Expect = 3e-177, Method: Compositional matrix adjust.
Identities = 244/312 (78%), Positives = 275/312 (88%), Gaps = 13/312 (4%)
Query 1 MTSNGANHLVPAS---GNTLGAP---KTDEDNGKPRVLLGKDAGGVSTWVPMEARLDNAT 54
+ ++GA+ +P S GN+ GAP K+ +GK +V AGG W M A + T
Sbjct 241 IDAHGAD--IPGSLIYGNSTGAPGEKKSMMFSGKEKVTNEMGAGG---W--MYADISEVT 293
Query 55 SATINQLRQAISVQQYYEALARGGSRYREQIRAIWDVIISDKTVQVPEYLGGGRYHVNIN 114
+ATIN LR+A++VQQYYEALARGGSRYREQ++A+WDV+ISDKT+Q+PEYLGGGRYHVN+N
Sbjct 294 AATINDLRKAVAVQQYYEALARGGSRYREQVQALWDVVISDKTMQIPEYLGGGRYHVNMN 353
Query 115 QIVQTSGQQTSNDTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHNRSYQQGLER 174
QIVQTSGQQT++DTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHN SYQQGLER
Sbjct 354 QIVQTSGQQTNDDTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHNHSYQQGLER 413
Query 175 FWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDDETFGYQEAWADYRMKPNRVSGLM 234
FWSR DRLDYYVPQFANLGEQP+KKKEIMLTG +D+ETFGYQEAWADYRMKPNRVSGLM
Sbjct 414 FWSRTDRLDYYVPQFANLGEQPIKKKEIMLTGTASDEETFGYQEAWADYRMKPNRVSGLM 473
Query 235 RSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIARTLIVEDEPQFFGAIRIANKTTRR 294
RSNA GTL+FWHYADNY+ VPTLSQEW+AEGKEEIARTLIV++EPQFFGAIRIANKTTRR
Sbjct 474 RSNAEGTLDFWHYADNYSTVPTLSQEWIAEGKEEIARTLIVQNEPQFFGAIRIANKTTRR 533
Query 295 MPLYSVPGLYKL 306
MPLYSVPGLYKL
Sbjct 534 MPLYSVPGLYKL 545
> Gokush_Human_feces_E_007_Microviridae_AG0412_putative.VP1
Length=573
Score = 468 bits (1203), Expect = 5e-164, Method: Compositional matrix adjust.
Identities = 220/261 (84%), Positives = 237/261 (91%), Gaps = 0/261 (0%)
Query 46 MEARLDNATSATINQLRQAISVQQYYEALARGGSRYREQIRAIWDVIISDKTVQVPEYLG 105
M ++LDN + TINQLRQA +VQ YYEALARGGSRYREQ+RA++ V ISDKTVQVPEYLG
Sbjct 313 MYSKLDNIEATTINQLRQAFAVQHYYEALARGGSRYREQVRALFGVSISDKTVQVPEYLG 372
Query 106 GGRYHVNINQIVQTSGQQTSNDTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHN 165
GGRYHVN+NQIVQTSGQQT+N+TPIGETGAMSVTPINESSFTKS EEHGF+IGV CVRH+
Sbjct 373 GGRYHVNVNQIVQTSGQQTTNETPIGETGAMSVTPINESSFTKSLEEHGFIIGVLCVRHD 432
Query 166 RSYQQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDDETFGYQEAWADYRM 225
SYQQGLERFWSR DRLDYY PQFANLGEQPVKKKEIMLTG TDDETFGYQEAWADYRM
Sbjct 433 HSYQQGLERFWSRSDRLDYYYPQFANLGEQPVKKKEIMLTGTATDDETFGYQEAWADYRM 492
Query 226 KPNRVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIARTLIVEDEPQFFGAI 285
KPNRVSG MRSNA GTL+FWHYADNY VPTLSQEWM EGK EIARTL+V+DEPQFFGAI
Sbjct 493 KPNRVSGKMRSNAEGTLDFWHYADNYDTVPTLSQEWMNEGKTEIARTLVVQDEPQFFGAI 552
Query 286 RIANKTTRRMPLYSVPGLYKL 306
R+ N+TTR MPLYSVPGL KL
Sbjct 553 RVMNETTRCMPLYSVPGLEKL 573
> Gokush_Human_feces_E_017_Microviridae_AG0127_putative.VP1
Length=557
Score = 360 bits (925), Expect = 2e-122, Method: Compositional matrix adjust.
Identities = 186/320 (58%), Positives = 218/320 (68%), Gaps = 24/320 (8%)
Query 2 TSNGANHLVPASGNTLGAPKTDEDNGKPRVLLGKDAGGVSTWVP---------------M 46
T+N A PASG T D+ + R L+G +A +P M
Sbjct 240 TANAA----PASGATAWYETQDKASNGQRTLIGGEAIDNGETLPLVTNFYGAANTGKAYM 295
Query 47 EARLDNATSATINQLRQAISVQQYYEALARGGSRYREQIRAIWDVIISDKTVQVPEYLGG 106
A L N T ATINQLRQA VQ+Y+E LARGGSRYREQI +++ ISDKTVQ+PEYLGG
Sbjct 296 GADLSNVTGATINQLRQAFQVQKYFEELARGGSRYREQIYSLFRTRISDKTVQIPEYLGG 355
Query 107 GRYHVNINQIVQTSGQQTSNDTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHNR 166
R +N+NQ++QTSG T+ +P G AMSVT +S+FTKSFEEHGFVIGVCCVRH+
Sbjct 356 DRIMINMNQVIQTSG--TTETSPQGNVAAMSVTGFGKSAFTKSFEEHGFVIGVCCVRHDH 413
Query 167 SYQQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDDETFGYQEAWADYRMK 226
+YQQGLER +SRK++LDYY P FANLGEQ V KKE+ G TD+E FGYQEAWADYRMK
Sbjct 414 TYQQGLERMFSRKNKLDYYFPVFANLGEQAVLKKELYAQGTETDNEAFGYQEAWADYRMK 473
Query 227 PNRVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIARTLIVE---DEPQFFG 283
PNR+ G RSNATGTL+ WHY DNY P LSQEWM EG EIARTL VE +EPQF
Sbjct 474 PNRICGAFRSNATGTLDTWHYGDNYTNTPALSQEWMKEGSAEIARTLAVENKVNEPQFIA 533
Query 284 AIRIANKTTRRMPLYSVPGL 303
+ N T R MP+YSVPGL
Sbjct 534 DFLVKNTTVRPMPMYSVPGL 553
> Gokush_Human_feces_A_020_Microviridae_AG0265_putative.VP1
Length=558
Score = 358 bits (919), Expect = 2e-121, Method: Compositional matrix adjust.
Identities = 177/273 (65%), Positives = 205/273 (75%), Gaps = 10/273 (4%)
Query 37 AGGV---STWVPMEARLDNATSATINQLRQAISVQQYYEALARGGSRYREQIRAIWDVII 93
+GG+ S W M A L AT ATINQLRQA VQ+Y+E LARGGSRYREQI +++ I
Sbjct 286 SGGLNNGSAW--MGADLSQATGATINQLRQAFQVQKYFEELARGGSRYREQIYSLFRTRI 343
Query 94 SDKTVQVPEYLGGGRYHVNINQIVQTSGQQTSNDTPIGETGAMSVTPINESSFTKSFEEH 153
SDKTVQ+PEYLGG R +N+NQ++QTSG T+ +P G AMSVT +S+FTKSFEEH
Sbjct 344 SDKTVQIPEYLGGDRIMINMNQVIQTSG--TTETSPQGNVAAMSVTGFGKSAFTKSFEEH 401
Query 154 GFVIGVCCVRHNRSYQQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDDET 213
GFVIGVCCVRH+ +YQQGLER +SRK++LDYY P FANLGEQ V KKE+ G TD+E
Sbjct 402 GFVIGVCCVRHDHTYQQGLERMFSRKNKLDYYFPVFANLGEQAVLKKELYAQGTDTDNEA 461
Query 214 FGYQEAWADYRMKPNRVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIARTL 273
FGYQEAWADYRMKPNR+ G RSNATGTL+ WHY DNY K P LSQEWM EG EIARTL
Sbjct 462 FGYQEAWADYRMKPNRICGAFRSNATGTLDSWHYGDNYTKTPALSQEWMKEGSAEIARTL 521
Query 274 IVE---DEPQFFGAIRIANKTTRRMPLYSVPGL 303
VE +EPQF + N T R MP+YSVPGL
Sbjct 522 AVENTVNEPQFIADFLVKNTTVRPMPMYSVPGL 554
> Gokush_Human_feces_A_019_Microviridae_AG0438_putative.VP1
Length=569
Score = 355 bits (912), Expect = 2e-120, Method: Compositional matrix adjust.
Identities = 174/279 (62%), Positives = 206/279 (74%), Gaps = 7/279 (3%)
Query 28 KPRVLLGKDAGGVSTWVPMEARLDNATSATINQLRQAISVQQYYEALARGGSRYREQIRA 87
K + A G S + M A L N T ATINQ+RQA VQ+Y+E LARGGSRYREQI +
Sbjct 291 KSTLYFANGASGGSAF--MGADLSNVTGATINQIRQAFQVQKYFEELARGGSRYREQIYS 348
Query 88 IWDVIISDKTVQVPEYLGGGRYHVNINQIVQTSGQQTSNDTPIGETGAMSVTPINESSFT 147
++ ISDKTVQ+PEYLGG R +N++Q+VQTSG T++ +P G AMSVT +S+FT
Sbjct 349 LFRTRISDKTVQIPEYLGGDRIMINMSQVVQTSG--TTDVSPQGNVSAMSVTGFGKSAFT 406
Query 148 KSFEEHGFVIGVCCVRHNRSYQQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGD 207
KSFEEHGF+IGVCCVRH+ +YQQGLER +SRK++LDYY P FANLGEQ V KKE+ G
Sbjct 407 KSFEEHGFIIGVCCVRHDHTYQQGLERMFSRKNKLDYYFPVFANLGEQAVLKKELYAQGT 466
Query 208 TTDDETFGYQEAWADYRMKPNRVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKE 267
TD+E FGYQE WADYRMKPNR+ G RSNATGTL+ WHY DNY+ P LSQEWM EG
Sbjct 467 ETDNEAFGYQECWADYRMKPNRICGAFRSNATGTLDSWHYGDNYSNTPALSQEWMKEGSA 526
Query 268 EIARTLIVE---DEPQFFGAIRIANKTTRRMPLYSVPGL 303
EIARTL VE +EPQF I + N T R MP+YSVPGL
Sbjct 527 EIARTLAVENKVNEPQFIADILVKNTTVRPMPMYSVPGL 565
> Gokush_Human_feces_B_029_Microviridae_AG0417_putative.VP1
Length=530
Score = 290 bits (743), Expect = 2e-95, Method: Compositional matrix adjust.
Identities = 145/292 (50%), Positives = 189/292 (65%), Gaps = 10/292 (3%)
Query 12 ASGNTLGAPKTDEDNGKPRVLLGKDAGGVSTWVPMEARLDNATSATINQLRQAISVQQYY 71
AS + K DE G A G+ + A L TSATINQLRQA +Q+
Sbjct 245 ASAEPIDVAKIDE--------TGTLAKGLPKASYIGADLSAVTSATINQLRQAFQIQKLL 296
Query 72 EALARGGSRYREQIRAIWDVIISDKTVQVPEYLGGGRYHVNINQIVQTSGQQTSNDTPIG 131
E ARGG+RYRE +R + VI D +Q+PEYLGG R +N++Q++QTS T + +P+G
Sbjct 297 EKDARGGTRYREVLREHFGVISPDSRMQIPEYLGGYRLPINVSQVIQTSS--TDSTSPLG 354
Query 132 ETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHNRSYQQGLERFWSRKDRLDYYVPQFAN 191
T A+SVT +N+ FTKSF EHGF++G+ VR +++YQQG+ER WSRK R DYY P AN
Sbjct 355 NTAALSVTTMNKPMFTKSFTEHGFIMGLAVVRTDQTYQQGIERMWSRKGRYDYYWPVLAN 414
Query 192 LGEQPVKKKEIMLTGDTTDDETFGYQEAWADYRMKPNRVSGLMRSNATGTLEFWHYADNY 251
+GEQ + KEI G D+E FGYQEAWADYR KP++V+ L RSNA +L+ WHYA +Y
Sbjct 415 IGEQAILNKEIYAQGSAKDEEAFGYQEAWADYRYKPSKVTALFRSNAQQSLDAWHYAQDY 474
Query 252 AKVPTLSQEWMAEGKEEIARTLIVEDEPQFFGAIRIANKTTRRMPLYSVPGL 303
++PTLS WM + E+ RTL D+P F NKTTR MP+YS+PGL
Sbjct 475 NELPTLSTAWMEQSNAEMKRTLARSDQPDFIADFYFMNKTTRCMPVYSIPGL 526
> Gokush_Human_feces_B_068_Microviridae_AG0331_putative.VP1
Length=547
Score = 281 bits (718), Expect = 1e-91, Method: Compositional matrix adjust.
Identities = 140/259 (54%), Positives = 173/259 (67%), Gaps = 4/259 (2%)
Query 48 ARLDNAT-SATINQLRQAISVQQYYEALARGGSRYREQIRAIWDVIISDKTVQVPEYLGG 106
A+ D + +ATINQLR A +Q+ YE ARGG+RY E +++ + V D +Q PEYLGG
Sbjct 286 AKFDGVSQAATINQLRLAFQIQKLYERDARGGTRYIEILKSHFGVTSPDARLQRPEYLGG 345
Query 107 GRYHVNINQIVQTSGQQTSNDTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHNR 166
R +NINQ+VQ+S S TP G T A S+T N S FTKSF EHGF+IGV R+
Sbjct 346 NRIPININQVVQSSSTDASG-TPQGNTAAYSLTSDNHSDFTKSFVEHGFLIGVMVARYRH 404
Query 167 SYQQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDDETFGYQEAWADYRMK 226
+YQQGLERFWSRKDR DYY P FAN+GEQ +K KEI G DDE FGYQEAWADYR +
Sbjct 405 TYQQGLERFWSRKDRFDYYFPVFANIGEQAIKNKEIYAQGTVKDDEVFGYQEAWADYRYR 464
Query 227 PNRVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIARTLIVED--EPQFFGA 284
PNRV+G MRS+A +L+ WH D+Y +P+LS W+ E + + R L V D Q F
Sbjct 465 PNRVTGEMRSSAPQSLDVWHLGDDYESLPSLSDSWIREDSKTVNRVLAVSDNVSAQLFCD 524
Query 285 IRIANKTTRRMPLYSVPGL 303
I + N TR MPLYS+PGL
Sbjct 525 IYVRNLCTRPMPLYSIPGL 543
> Gokush_Human_feces_E_009_Microviridae_AG0285_putative.VP1
Length=559
Score = 276 bits (706), Expect = 1e-89, Method: Compositional matrix adjust.
Identities = 137/258 (53%), Positives = 167/258 (65%), Gaps = 3/258 (1%)
Query 48 ARLDNATSATINQLRQAISVQQYYEALARGGSRYREQIRAIWDVIISDKTVQVPEYLGGG 107
A L ATSATINQLR A +Q+ YE ARGG+RY E ++ + V D +Q PEYLGG
Sbjct 299 ADLSTATSATINQLRLAFQIQKLYEKDARGGTRYTEILKTHFGVTSPDSRLQRPEYLGGN 358
Query 108 RYHVNINQIVQTSGQQTSNDTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHNRS 167
R +NINQ+VQ S +TP+G SVT S F +SF EHGFVIGV R++ +
Sbjct 359 RVPININQVVQNSAT-VEGETPLGNVAGYSVTSDTHSDFRQSFTEHGFVIGVMVARYDHT 417
Query 168 YQQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDDETFGYQEAWADYRMKP 227
YQQG+ERFWSRK R DYY P AN+GEQ V KEI G DDE FGYQEAW DYR KP
Sbjct 418 YQQGIERFWSRKTRFDYYWPVLANIGEQAVLNKEIYAQGTAEDDEVFGYQEAWGDYRYKP 477
Query 228 NRVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIARTLIV--EDEPQFFGAI 285
NRV+G MRS +L+ WH D+Y K+P+LS EW+ E K + R L V + Q F +
Sbjct 478 NRVTGEMRSQYAQSLDVWHLGDDYTKLPSLSSEWIVEDKTNVDRVLAVTSTNANQLFADL 537
Query 286 RIANKTTRRMPLYSVPGL 303
I N+TTR MP+YS+PGL
Sbjct 538 YINNQTTRPMPMYSIPGL 555
> Gokush_Human_feces_A_029_Microviridae_AG0226_putative.VP1
Length=470
Score = 272 bits (696), Expect = 3e-89, Method: Compositional matrix adjust.
Identities = 143/274 (52%), Positives = 175/274 (64%), Gaps = 16/274 (6%)
Query 32 LLGKDAGGVSTWVPMEARLDNATSATINQLRQAISVQQYYEALARGGSRYREQIRAIWDV 91
L+ D GGVS ATINQLR A +Q+ YE ARGG+RY E +++ + V
Sbjct 207 LIAVDDGGVSM-------------ATINQLRLAFQIQKLYERDARGGTRYIEILKSHFGV 253
Query 92 IISDKTVQVPEYLGGGRYHVNINQIVQTSGQQTSNDTPIGETGAMSVTPINESSFTKSFE 151
D +Q PEYLGG R + INQ+VQ SG S +TP G T A S+T FTKSF
Sbjct 254 TSPDARLQRPEYLGGNRIPITINQVVQNSGTM-SGETPQGTTTAYSLTTDVHQEFTKSFV 312
Query 152 EHGFVIGVCCVRHNRSYQQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDD 211
EHGF+IGV R++ +YQQGLERFWSRKDR D+Y P FAN+GEQ + KEI TG +D
Sbjct 313 EHGFIIGVMVARYDHTYQQGLERFWSRKDRFDFYWPVFANIGEQAILNKEIYATGKDSDS 372
Query 212 ETFGYQEAWADYRMKPNRVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIAR 271
E FGYQEAWADYR KP+RVSG MRSNA L+ WH AD+Y +PTLS W+ E + R
Sbjct 373 EVFGYQEAWADYRYKPSRVSGEMRSNAKTPLDSWHLADDYDVLPTLSDSWIREESNNVNR 432
Query 272 TLIVEDE--PQFFGAIRIANKTTRRMPLYSVPGL 303
L V E Q F + + N+TTR MP+YS+PGL
Sbjct 433 VLAVTSEVSNQLFCDLYVQNRTTRPMPVYSIPGL 466
> Gokush_Human_feces_A_013_Microviridae_AG009_putative.VP1
Length=587
Score = 272 bits (696), Expect = 6e-88, Method: Compositional matrix adjust.
Identities = 136/257 (53%), Positives = 171/257 (67%), Gaps = 4/257 (2%)
Query 51 DNATS-ATINQLRQAISVQQYYEALARGGSRYREQIRAIWDVIISDKTVQVPEYLGGGRY 109
D A S ATINQLR A +Q++YE ARGGSRY E IR+ + V D +Q PEYLGG R
Sbjct 327 DGAVSVATINQLRLAFQIQKFYERQARGGSRYTEVIRSFFGVTSPDARLQRPEYLGGNRV 386
Query 110 HVNINQIVQ-TSGQQTSNDTPIGETGAMSVTPINESSFTKSFEEHGFVIGVCCVRHNRSY 168
+NINQ++Q + S+ TP G MS T S FTKSF EHGF+IGV R++ +Y
Sbjct 387 PININQVIQQSGTGSESSSTPQGTVVGMSQTTDTNSDFTKSFTEHGFIIGVMVARYDHTY 446
Query 169 QQGLERFWSRKDRLDYYVPQFANLGEQPVKKKEIMLTGDTTDDETFGYQEAWADYRMKPN 228
QQGL+R WSRKD+ D+Y P FAN+GEQ +K KE+ G DDE FGYQEAWA+YR KPN
Sbjct 447 QQGLDRLWSRKDKFDFYWPVFANIGEQAIKNKELYAQGTAEDDEVFGYQEAWAEYRYKPN 506
Query 229 RVSGLMRSNATGTLEFWHYADNYAKVPTLSQEWMAEGKEEIARTLIVEDE--PQFFGAIR 286
RV+G MRS+ +L+ WH AD+Y+K+P+LS EW+ E + R L D QFF I
Sbjct 507 RVTGEMRSSYAKSLDIWHLADDYSKLPSLSAEWIQEDSSTVNRVLAASDNLAAQFFADIY 566
Query 287 IANKTTRRMPLYSVPGL 303
+ N TR MP+YS+PGL
Sbjct 567 VKNLCTRPMPMYSIPGL 583
Lambda K H a alpha
0.315 0.132 0.395 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 25785158