bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-7_CDS_annotation_glimmer3.pl_2_1
Length=277
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 479 2e-172
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 460 5e-165
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 434 7e-155
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 421 2e-150
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 415 2e-147
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 256 3e-85
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 207 2e-66
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 196 2e-61
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 194 5e-61
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 184 4e-57
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 479 bits (1232), Expect = 2e-172, Method: Compositional matrix adjust.
Identities = 250/277 (90%), Positives = 265/277 (96%), Gaps = 0/277 (0%)
Query 1 MSEELRTEPNAYFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIKH 60
MSEELRTEPNAYFMTLTISDENYEILKN CKSED+NTIATKAIRL LERIRKK GKSI+H
Sbjct 56 MSEELRTEPNAYFMTLTISDENYEILKNICKSEDENTIATKAIRLMLERIRKKIGKSIRH 115
Query 61 WFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKID 120
WFITELGHEKTERLHLHGIVWGIGTDQLI+EKWNYGITYTGNFVNEKTINYITKYMTKID
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNEKTINYITKYMTKID 175
Query 121 EDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLFT 180
E+HP FVGKVLCSKGIGAGY KR DA+KHKY+KG+TIETYRLRNGAKINLPIYYRNKLFT
Sbjct 176 EEHPNFVGKVLCSKGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFT 235
Query 181 EEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQKYI 240
E+ERELLFIDKI+KG IYVLGTKVHRDDE+YYIQLLEEGRKKE +LYG H Q+WEQQKY+
Sbjct 236 EKERELLFIDKIDKGIIYVLGTKVHRDDEKYYIQLLEEGRKKENMLYGNHTQEWEQQKYL 295
Query 241 nrlrkqkkkqeqelnqleIYWALERAKDYTQLETCPF 277
NRL+KQKKKQEQE+NQLE YWALERAKDYTQLE CPF
Sbjct 296 NRLQKQKKKQEQEINQLETYWALERAKDYTQLEECPF 332
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 460 bits (1183), Expect = 5e-165, Method: Compositional matrix adjust.
Identities = 236/277 (85%), Positives = 258/277 (93%), Gaps = 0/277 (0%)
Query 1 MSEELRTEPNAYFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIKH 60
MSEELRTEPNAYFMTLTISDENYEILKN CKSED NTIATKAIRL LERIRKKTGKSIKH
Sbjct 56 MSEELRTEPNAYFMTLTISDENYEILKNICKSEDDNTIATKAIRLMLERIRKKTGKSIKH 115
Query 61 WFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKID 120
WFITELGHEKTERLHLHGIVWGIGTDQLI+EKWNYGITYTGN+VNEKTINY+TKYMTKID
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNEKTINYVTKYMTKID 175
Query 121 EDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLFT 180
E HP+FVGKVLCS+GIGAGY KR DA+KHKY+KG+TIETYRLRNGAKINLPIYYRNKLFT
Sbjct 176 EKHPDFVGKVLCSRGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFT 235
Query 181 EEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQKYI 240
E+ERELLFIDKIEKG IYV+G KVHRDDEEYY+QLL+EGRK E LYG + Q+WE+QKY+
Sbjct 236 EKERELLFIDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRLYGYNLQNWEKQKYL 295
Query 241 nrlrkqkkkqeqelnqleIYWALERAKDYTQLETCPF 277
+RLR+QKKKQ++EL QLE YWA+ RAK YT L+ CPF
Sbjct 296 SRLRRQKKKQDKELQQLETYWAINRAKKYTSLDECPF 332
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 434 bits (1116), Expect = 7e-155, Method: Compositional matrix adjust.
Identities = 220/277 (79%), Positives = 243/277 (88%), Gaps = 0/277 (0%)
Query 1 MSEELRTEPNAYFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIKH 60
MSEELR PNAYFMTLTI DENY L N C S+D N IATKA+RL LERIRKKTGKSIKH
Sbjct 56 MSEELRQNPNAYFMTLTIDDENYNKLANICNSKDNNEIATKAVRLMLERIRKKTGKSIKH 115
Query 61 WFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKID 120
WFITELGHEKTERLHLHGIVWGIGTDQLI EKWNYG YTGNFVNE TINYITKYMTK+D
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNEATINYITKYMTKVD 175
Query 121 EDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLFT 180
DHP+FVG+VLCSKGIGAGY KR DA+ HKY KGKT ETYRLRNGAKINLPIYYRN+LF+
Sbjct 176 IDHPDFVGQVLCSKGIGAGYTKREDANNHKYTKGKTNETYRLRNGAKINLPIYYRNQLFS 235
Query 181 EEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQKYI 240
EEERE+LF+DKIEKG IYV+G KVHRD+E Y+ LLEE RK E LYG H Q+WE+QKY+
Sbjct 236 EEEREMLFLDKIEKGIIYVMGQKVHRDNEAEYLGLLEEARKTEQRLYGVHEQEWEEQKYL 295
Query 241 nrlrkqkkkqeqelnqleIYWALERAKDYTQLETCPF 277
NRLR+Q+K+QE++ +LE+YWALERAKDYTQLETCPF
Sbjct 296 NRLRRQRKRQERKTKELEMYWALERAKDYTQLETCPF 332
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 421 bits (1082), Expect = 2e-150, Method: Compositional matrix adjust.
Identities = 218/277 (79%), Positives = 243/277 (88%), Gaps = 0/277 (0%)
Query 1 MSEELRTEPNAYFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIKH 60
MSEELR EPNAYF+TLTI D++Y L NT D N IATKAIRL LERIRK+TGKSIKH
Sbjct 15 MSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKSIKH 74
Query 61 WFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKID 120
WFITELGHEKTERLHLHGIVWGIGTD+LI KWNYGIT+TG FVNEKTI YITKYMTKID
Sbjct 75 WFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNEKTIQYITKYMTKID 134
Query 121 EDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLFT 180
E H +F+GKVLCSKGIGAGYIKR DA KH Y+ G+TIETYRLRNG+KINLPIYYRN+LFT
Sbjct 135 EQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGSKINLPIYYRNQLFT 194
Query 181 EEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQKYI 240
EEE+E LF+DKIEKG IYV+G KVHRDDEEYY+QLL EGRK E LYG + Q+WEQQKY+
Sbjct 195 EEEKEALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLNEGRKTECRLYGYNLQNWEQQKYL 254
Query 241 nrlrkqkkkqeqelnqleIYWALERAKDYTQLETCPF 277
+RLR+QKK+Q+++L QLE YWALER KDYTQLETCPF
Sbjct 255 SRLRRQKKRQDKDLQQLETYWALERTKDYTQLETCPF 291
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 415 bits (1067), Expect = 2e-147, Method: Compositional matrix adjust.
Identities = 213/277 (77%), Positives = 244/277 (88%), Gaps = 0/277 (0%)
Query 1 MSEELRTEPNAYFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIKH 60
MSEELR EPNAYF+TLTI D++Y L NT D N IATKAIRL LERIRK+TGKSIKH
Sbjct 56 MSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKSIKH 115
Query 61 WFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKID 120
WFITELGHEKTERLHLHGIVWGIGTD+LI KWNYGIT+TG FVNEKTI YITKYMTKID
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNEKTIQYITKYMTKID 175
Query 121 EDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLFT 180
E H +F+GKVLCSKGIGAGYIKR DA KH Y++G+TIETYRLRNG+KINLPIYYRN+LFT
Sbjct 176 EQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKRGETIETYRLRNGSKINLPIYYRNQLFT 235
Query 181 EEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQKYI 240
EEERE LF+DKIEKG IYV+G KVHRDDEEYY+QLL+EGRK E LYG + Q+WEQQKY+
Sbjct 236 EEEREALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRLYGYNLQNWEQQKYL 295
Query 241 nrlrkqkkkqeqelnqleIYWALERAKDYTQLETCPF 277
+RLR+QKK+Q+++L QLE YWA+E++K+YT L+ CPF
Sbjct 296 SRLRRQKKRQDKDLQQLETYWAIEKSKEYTSLDKCPF 332
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 256 bits (653), Expect = 3e-85, Method: Compositional matrix adjust.
Identities = 125/239 (52%), Positives = 169/239 (71%), Gaps = 0/239 (0%)
Query 1 MSEELRTEPNAYFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIKH 60
+SEE+R++PNA F+TLTISDE++E +KNT K +RL LER+RKKT KS+KH
Sbjct 56 LSEEVRSDPNAIFVTLTISDESWEKIKNTYIQLSDEDCIKKMVRLFLERVRKKTKKSLKH 115
Query 61 WFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKID 120
W TE G TER HLHG++WG T+ L K W YG + G FVNE T+NYITKY+TK D
Sbjct 116 WLTTERGGTNTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNECTVNYITKYITKTD 175
Query 121 EDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLFT 180
+ H +F LCS GIG GY+ R+D+ +++ +GKT ETYRLRNG K+NLPIYYRNKL+T
Sbjct 176 KKHKDFEPITLCSAGIGKGYLSRSDSELNRFREGKTTETYRLRNGTKLNLPIYYRNKLYT 235
Query 181 EEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQKY 239
+EERE LF++KI+KG +++ G + + D + Y Q+L+E + K L+G+ DW+ KY
Sbjct 236 DEEREKLFLEKIKKGKVWICGRECNIKDWKTYSQILKEEQIKAKQLHGDSPIDWDIAKY 294
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 207 bits (528), Expect = 2e-66, Method: Compositional matrix adjust.
Identities = 114/246 (46%), Positives = 162/246 (66%), Gaps = 12/246 (5%)
Query 1 MSEELRTEPNAYFMTLTISDENYEIL--------KNTCKSEDKNTIATKAIRLTLERIRK 52
++EEL+ PNA F+TLT ++ENY+ L K+ ++N + A+R LERIRK
Sbjct 54 LAEELKNNPNALFITLTFNEENYQRLSWELFKKSKDNLNYTEQNEMCKTAVRRWLERIRK 113
Query 53 KTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYI 112
KT KSI+HW +TE G E R+HLHGIVW +++ E+W YG TY G++VNE TI Y+
Sbjct 114 KTKKSIRHWMVTEKG-EDYGRIHLHGIVW-CPKERI--EQWGYGYTYIGDYVNETTIAYV 169
Query 113 TKYMTKIDEDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPI 172
TKYM KI E P+F GKV+CS GIG Y +A +++Y +T ETY+L NG ++ LP
Sbjct 170 TKYMLKICEKWPDFRGKVMCSAGIGCRYETSYNAKRNRYRGKETKETYKLENGRELPLPK 229
Query 173 YYRNKLFTEEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQ 232
YY +K++TEEERE L+I K E+G+ Y+ G KV D+ E + L + +K+ LYG+ +
Sbjct 230 YYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTDNLEEWDNLTKYYQKRAEQLYGDKPE 289
Query 233 DWEQQK 238
DWE++K
Sbjct 290 DWEREK 295
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 196 bits (499), Expect = 2e-61, Method: Compositional matrix adjust.
Identities = 110/233 (47%), Positives = 145/233 (62%), Gaps = 7/233 (3%)
Query 11 AYFMTLTISDENYEILKNTCK---SEDKNTIATKAIRLTLERIRKKTGKSIKHWFITELG 67
YF TLTI EN + +++ E+ N IATKA+RL LER RK TGKSI+HW +TELG
Sbjct 65 GYFTTLTIDQENIKKIESITGLKWEENPNEIATKALRLFLERTRKDTGKSIRHWCVTELG 124
Query 68 HEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKIDEDHPEFV 127
EK R+HLHGI +G + +LI++ WNYG + G + N K+INYITKYM K+D HPEF
Sbjct 125 -EKNNRIHLHGIFFGQKSAELIRKHWNYGFIFIGGYCNSKSINYITKYMLKVDIKHPEFK 183
Query 128 GKVLCSKGIGAGYIKRAD--ASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLFTEEERE 185
VL S GIG GYI R D K Y K + TY RNG K+ +P YY+NK+FTE+ERE
Sbjct 184 QIVLASSGIGKGYIDRLDYLWQKQNY-KNINVATYTFRNGTKMAMPKYYKNKIFTEKERE 242
Query 186 LLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQK 238
++I+ + +G +++ G KV DD E L E +K G++ W K
Sbjct 243 KMWINNLNRGLLWIYGEKVKADDWETIDNLREYWQKYGRETMGDNPIAWNAMK 295
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 194 bits (492), Expect = 5e-61, Method: Compositional matrix adjust.
Identities = 110/240 (46%), Positives = 155/240 (65%), Gaps = 7/240 (3%)
Query 1 MSEELRTEPNA-YFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIK 59
+SEELR+ A F+TLT ++E+Y+ L K+E N + A+ E R+K +I+
Sbjct 56 LSEELRSGKGAGLFVTLTFNEESYKELAAITKNE--NDMCRLALYRMNENYRQKYKHTIR 113
Query 60 HWFITELGHEKTERLHLHGIVWGIGTDQLIKEKWNYGITYTGNFVNEKTINYITKYMTKI 119
HW +TE+G + R+H+HGI+W +D I+ W YG Y G FVNE+TI YITKYM K
Sbjct 114 HWCVTEIGDDG--RIHIHGIMWCPASD--IERYWKYGYIYIGRFVNEQTILYITKYMLKY 169
Query 120 DEDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRNKLF 179
F KVLCSKGIG Y+ R D+ ++ Y + T E+Y LR+G KINLP YY+ K++
Sbjct 170 TPVDKNFEPKVLCSKGIGINYLDRLDSKRNTYRENNTDESYMLRSGRKINLPDYYKRKIY 229
Query 180 TEEERELLFIDKIEKGFIYVLGTKVHRDDEEYYIQLLEEGRKKEAILYGEHNQDWEQQKY 239
TEEERE L+I+K EKG+ Y++G KV D+EE +L+E RKK LY E Q+W+++K+
Sbjct 230 TEEEREKLWIEKQEKGYRYIMGEKVSTDNEEKVYKLMEYWRKKAKELYNEKPQEWDREKH 289
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 184 bits (468), Expect = 4e-57, Method: Compositional matrix adjust.
Identities = 110/240 (46%), Positives = 143/240 (60%), Gaps = 21/240 (9%)
Query 3 EELRTEPNAYFMTLTISDENYEILKNTCKSEDKNTIATKAIRLTLERIRKKTGKSIKHWF 62
E+LR P A F T T+S E Y+ +K + N I TK RL LERIRK+TGKS+KHW
Sbjct 58 EQLRETPTAIFFTGTVSPERYDYIKEKYNLKTDNEIITKIHRLFLERIRKETGKSMKHWC 117
Query 63 ITELGHEKTERLHLHGIVWGI-GTDQ-----LIKEKWNYGITYTGNFVNEKTINYITKYM 116
+TE GH T R+HLHGI + G Q +++ W G Y G + NEKTINY++KYM
Sbjct 118 VTEKGHTNTRRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYNGKYCNEKTINYVSKYM 177
Query 117 TKIDEDHPEFVGKVLCSKGIGAGYIKRADASKHKYEKGKTIETYRLRNGAKINLPIYYRN 176
TK D D+PE+ GKVLCS G+GAGY+KR +H++ + T E Y R G I LP YY+
Sbjct 178 TKKDMDNPEYTGKVLCSPGLGAGYVKRI-GKRHEWNEENTKEDYYTRQGTYIALPKYYKY 236
Query 177 KLFTEEERELLFIDKIEKGFIYVLGTKVHRDD---EEYYIQLLEEGRKKEAILYGEHNQD 233
KLFTE++RE L+I + G +V KV D EEYY +L +HN+D
Sbjct 237 KLFTEDQREQLWIYRENSGEKFVGNFKVKITDEESEEYY-----------NVLKKQHNED 285
Lambda K H a alpha
0.317 0.137 0.407 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 22872360