bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-8_CDS_annotation_glimmer3.pl_2_1
Length=423
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_C_010_Microviridae_AG0198_putative.VP4 821 0.0
Alpavirinae_Human_gut_21_005_Microviridae_AG013_putative.VP4 818 0.0
Alpavirinae_Human_feces_B_007_Microviridae_AG067_putative.VP4 150 5e-42
Pichovirinae_59_Coral_002_Microviridae_AG0338_putative.VP4 91.7 9e-23
Gokush_Human_feces_A_029_Microviridae_AG0222_putative.VP4 90.1 8e-22
Gokush_Human_feces_B_068_Microviridae_AG0328_putative.VP4 89.7 1e-21
Pichovirinae_JCVI_003_Microviridae_AG0343_putative.VP4 87.4 3e-21
Gokush_gi|9634955|ref|NP_054653.1|_nonstructural_protein_[Chlam... 87.4 6e-21
Gokush_gi|17402855|ref|NP_510879.1|_hypothetical_protein_PhiCPG... 85.1 1e-20
Gokush_gi|77020121|ref|YP_338244.1|_putative_replication_protei... 85.1 3e-20
> Alpavirinae_Human_feces_C_010_Microviridae_AG0198_putative.VP4
Length=451
Score = 821 bits (2120), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 401/423 (95%), Positives = 411/423 (97%), Gaps = 0/423 (0%)
Query 1 MPSGTYHGSVLhvnknhvnknnIDKYTIVNPATGETFPMFLIVPCNKCALCNEKKAQQWS 60
MPSG Y+GSVLHVNKNHVNKNNIDKYT+VNPATGETFPMFLIVPCNKCALCNEKKAQQWS
Sbjct 29 MPSGVYYGSVLHVNKNHVNKNNIDKYTVVNPATGETFPMFLIVPCNKCALCNEKKAQQWS 88
Query 61 FRALCESYTSNKQAYFITLTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIA 120
FRALCESYTSNKQAYFITLTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIA
Sbjct 89 FRALCESYTSNKQAYFITLTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIA 148
Query 121 VSEYGHWSKRPHYHIILWNFPDNFESAYSRLTLIESCWRRPTGEYHPDGSPVTRSIGFAY 180
VSEYGHWSKRPHYHIILWNFPDNFESAYSRLTLIESCWRRPTGEY+PDGSPVTRSIGFAY
Sbjct 149 VSEYGHWSKRPHYHIILWNFPDNFESAYSRLTLIESCWRRPTGEYNPDGSPVTRSIGFAY 208
Query 181 CVPVINGGINYVMKYMGKRECAPEGMNSTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCD 240
CVPVINGGINYVMKY+GKRECAPEGMN TFMLASRKNGGIGSAYAEQLRAFYEQQPDTCD
Sbjct 209 CVPVINGGINYVMKYLGKRECAPEGMNHTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCD 268
Query 241 MSVINIYTGQPLTTMLPRYYRMKFMPSTSMCYDSNFIKYFKDTVRWFEIARYLHKQYKLP 300
MSVINIYTGQ LTTMLPRYYRMKFMPSTSMCYD NFIKYFKDTVRWFEIARYLHKQYKLP
Sbjct 269 MSVINIYTGQSLTTMLPRYYRMKFMPSTSMCYDPNFIKYFKDTVRWFEIARYLHKQYKLP 328
Query 301 FKFTYPEEYLRLVRMTGKTPYYDPSKTIINDTFIKYYLPQFCSQTVYEDLYYKAFSYGLD 360
FKFTYPEEYLRLVRMTGKTPYY+P KT++NDTFIKYYLPQF SQ VYEDLY K+FSY LD
Sbjct 329 FKFTYPEEYLRLVRMTGKTPYYNPYKTVMNDTFIKYYLPQFLSQAVYEDLYCKSFSYALD 388
Query 361 CLSTALVFFDSSQVLKHEKSLHMNSLQQAAINARMSMREELNLKKASYDVRVKMNKHYRK 420
CLSTALVFFDSSQVLKHEKSL MNSLQQAAINARM+ REELN+KKASYDVR KMNKHYRK
Sbjct 389 CLSTALVFFDSSQVLKHEKSLQMNSLQQAAINARMATREELNIKKASYDVREKMNKHYRK 448
Query 421 EKI 423
EKI
Sbjct 449 EKI 451
> Alpavirinae_Human_gut_21_005_Microviridae_AG013_putative.VP4
Length=451
Score = 818 bits (2113), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 397/423 (94%), Positives = 412/423 (97%), Gaps = 0/423 (0%)
Query 1 MPSGTYHGSVLhvnknhvnknnIDKYTIVNPATGETFPMFLIVPCNKCALCNEKKAQQWS 60
MPSG YHGSVLHVNKNHVNKNNIDKYTIVNPATGETFPMFLIVPCNKC LCNEKKAQQWS
Sbjct 29 MPSGVYHGSVLHVNKNHVNKNNIDKYTIVNPATGETFPMFLIVPCNKCVLCNEKKAQQWS 88
Query 61 FRALCESYTSNKQAYFITLTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIA 120
FRALCESYTSNKQAYFITLTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIA
Sbjct 89 FRALCESYTSNKQAYFITLTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIA 148
Query 121 VSEYGHWSKRPHYHIILWNFPDNFESAYSRLTLIESCWRRPTGEYHPDGSPVTRSIGFAY 180
VSEYGHWSKRPHYHIILWNFPDNFESAYSRLTLIESCWRRPTG+Y+PDGSPVTRSIGFAY
Sbjct 149 VSEYGHWSKRPHYHIILWNFPDNFESAYSRLTLIESCWRRPTGKYNPDGSPVTRSIGFAY 208
Query 181 CVPVINGGINYVMKYMGKRECAPEGMNSTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCD 240
CVPVINGGINYVMKYMGKRE +PEGMN TFMLASRKNGGIGSAYAEQLRAFYEQQPDTCD
Sbjct 209 CVPVINGGINYVMKYMGKREKSPEGMNPTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCD 268
Query 241 MSVINIYTGQPLTTMLPRYYRMKFMPSTSMCYDSNFIKYFKDTVRWFEIARYLHKQYKLP 300
MSVINIYTGQ LTTMLPRYYRMKFMPS SMCYD NFIK+FKDTVRWFE ARYLHKQYKLP
Sbjct 269 MSVINIYTGQTLTTMLPRYYRMKFMPSPSMCYDPNFIKHFKDTVRWFETARYLHKQYKLP 328
Query 301 FKFTYPEEYLRLVRMTGKTPYYDPSKTIINDTFIKYYLPQFCSQTVYEDLYYKAFSYGLD 360
FKFTYPEEYLRL+RMT KTPYY+PS+ I+NDTFIKYYLPQFCSQTVYEDLYYKAFSYGLD
Sbjct 329 FKFTYPEEYLRLIRMTNKTPYYNPSRNIVNDTFIKYYLPQFCSQTVYEDLYYKAFSYGLD 388
Query 361 CLSTALVFFDSSQVLKHEKSLHMNSLQQAAINARMSMREELNLKKASYDVRVKMNKHYRK 420
CL+T+LVFFDSSQVLKHEKSLHMNSLQQ+AINARM+++EELNL+KASYDVR KMNKHYRK
Sbjct 389 CLATSLVFFDSSQVLKHEKSLHMNSLQQSAINARMALKEELNLEKASYDVREKMNKHYRK 448
Query 421 EKI 423
EKI
Sbjct 449 EKI 451
> Alpavirinae_Human_feces_B_007_Microviridae_AG067_putative.VP4
Length=515
Score = 150 bits (380), Expect = 5e-42, Method: Compositional matrix adjust.
Identities = 98/309 (32%), Positives = 146/309 (47%), Gaps = 49/309 (16%)
Query 23 IDKYTIVNPATGETFPMFLIVPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYN 82
I+ ++N TG+ P++++VPC C +C ++KA + RA+ E+ T+ FITLTYN
Sbjct 59 IEHCYLLNSETGDMIPLYIVVPCGSCVICRKRKANALATRAIMETETTGSAPLFITLTYN 118
Query 83 NEHLPKN-----GVFPEEIQLFFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIIL 137
EHLPKN + ++QLFFKRLR+ LD + ISH+LRY+A EYG +KRPHYH++L
Sbjct 119 PEHLPKNEYGYETLRKSDLQLFFKRLRSLLDNQSISHSLRYLACGEYGTNTKRPHYHLLL 178
Query 138 WNFPDN-----------FESAYS---------RLTLIESCWRRPTGEY------------ 165
W FP + A+S R+ C P +Y
Sbjct 179 WGFPATHFKDILKVQAFIQKAWSYFQSDENGKRIPYYSKCRSCPLNQYKDRNSCSAVAHL 238
Query 166 -------HPDGSPVTRS--IGFAYCVPVINGGINYVMKYMGKRECAPEGMNST-FMLASR 215
+P G+ + R IG +P +G Y+ KYM K AP F +AS
Sbjct 239 CAGARLRYPSGAFIYRRYPIGSIKVLPANSGAPAYITKYMVKGSNAPHSTCELPFRVASN 298
Query 216 KNGGIGSAYAEQLRAFYEQQPDTCDMSVINIYTGQPLTTMLP--RYYRMKFMPSTSMCYD 273
+ GGIGSAY + + V++ TG +P + + +PS S C
Sbjct 299 RGGGIGSAYIRARKDEILSNSSLEALPVVDRVTGSGKIFYMPIDSWVKSTLIPSPSSCLK 358
Query 274 SNFIKYFKD 282
+ + +D
Sbjct 359 TKEYETVRD 367
> Pichovirinae_59_Coral_002_Microviridae_AG0338_putative.VP4
Length=274
Score = 91.7 bits (226), Expect = 9e-23, Method: Compositional matrix adjust.
Identities = 76/240 (32%), Positives = 112/240 (47%), Gaps = 41/240 (17%)
Query 39 MFLIVPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYNNEHLPKNG---VFPEE 95
+ + VPC KC C +++A QWSFR E+ TS+ A FITLTY N + +NG + +
Sbjct 21 LMVSVPCGKCLACTKRRASQWSFRLNEEAKTSS-SACFITLTYENAPVSENGFRTLNKRD 79
Query 96 IQLFFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILWNFPDNFESAYSRLTLIE 155
QLF KRLR K ++ L+Y A EYG + RPHYH I++N P +LI+
Sbjct 80 FQLFLKRLRKKCP----TNKLKYYACGEYGTRTFRPHYHAIIFNLPK---------SLIQ 126
Query 156 SCWRRPTGEYHPDGSPVTRSIGFAYCVPVINGGINYVMKYMGK----RECAPEGMNSTFM 211
P T + G + INYV+ YM K R + F
Sbjct 127 D----------PQKIVDTWTHGHIHLANNNQSTINYVVGYMTKGNFERFNDQDDRMPEFS 176
Query 212 LASRKNGGIGSAY-AEQLRAFYEQQPDTCDMSVINIYTGQPLTTMLPRYYRMKFMPSTSM 270
L S+K +G Y Q++ +Y ++ TC + G ++ +PRYY+ K +
Sbjct 177 LMSKK---MGMGYLTPQMKEYYRKREITCLVRE----NGHIIS--MPRYYKEKIFTKQEL 227
> Gokush_Human_feces_A_029_Microviridae_AG0222_putative.VP4
Length=343
Score = 90.1 bits (222), Expect = 8e-22, Method: Compositional matrix adjust.
Identities = 72/251 (29%), Positives = 109/251 (43%), Gaps = 57/251 (23%)
Query 43 VPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYNNEHLPKN---------GVFP 93
+PC +C C + ++QW+ R + E + AYF+T+TY++EH+P+ + P
Sbjct 57 IPCGRCIGCRLEYSRQWANRCMLE-LQYHDSAYFVTVTYDDEHVPQTYSSDSETGEALLP 115
Query 94 ------EEIQLFFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILWN-------- 139
++QLF KR+R +R +RY EYG + RPHYH IL+
Sbjct 116 LMTLSKRDMQLFMKRVR----KRFCDDRIRYFLAGEYGSTTFRPHYHCILFGLHLYDLVP 171
Query 140 FPDNF--ESAYSRLTLIESCWRRPTGEYHPDGSPVTRSIGFAYCVPVINGGINYVMKYMG 197
+ NF + Y+ +L +CW R +G+ PV YV +Y
Sbjct 172 YAKNFRGDVLYNSQSL-SACW----------CDKSARPMGYVVVAPVTYETCAYVARYTS 220
Query 198 KRECAPE-------GMNSTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCDMSVINIYTGQ 250
K+ + G+ F L SRK GIG R +++ PD D IN+ TG
Sbjct 221 KKSGVNDLEAYDLLGLARPFTLMSRKP-GIG-------RQYFDDHPDCMDYDFINVSTGD 272
Query 251 PLTTML-PRYY 260
PRYY
Sbjct 273 GGKKFHPPRYY 283
> Gokush_Human_feces_B_068_Microviridae_AG0328_putative.VP4
Length=351
Score = 89.7 bits (221), Expect = 1e-21, Method: Compositional matrix adjust.
Identities = 69/243 (28%), Positives = 104/243 (43%), Gaps = 59/243 (24%)
Query 41 LIVPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYNNEHLPKN----------- 89
+I+PC KC C + ++QW+ R + E + ++F+TLTY++ HLP++
Sbjct 60 VIIPCGKCLGCRLEYSRQWANRCMLE-LGYHVSSWFVTLTYDDAHLPRSFYGNPDTGEAV 118
Query 90 ---GVFPEEIQLFFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILWNFPDNFES 146
++ + QLF KRLR K +R+ A EYG +KRPHYH I++ +
Sbjct 119 PCATLYKRDFQLFMKRLRYKF-----GDGIRFYAAGEYGDQTKRPHYHAIIYGLELDDLV 173
Query 147 AYSRLTL--------------IESCWRRPTGEYHPDGSPVTRSIGFAYCVPVINGGINYV 192
Y ++ L ++SCWR G IGF V YV
Sbjct 174 FYKKMALESANLYYNYYNSESLQSCWRDKDGN----------DIGFVVVGKVTWETCAYV 223
Query 193 MKYMGKRECAPEG-------MNSTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCDMSVIN 245
+Y+ K++ + F L SRK G A Q +YE P+ D IN
Sbjct 224 ARYIMKKQKGQGADVYERFNIEPEFCLMSRKPG-----IAHQ---YYEDHPEMWDYDKIN 275
Query 246 IYT 248
I T
Sbjct 276 IST 278
> Pichovirinae_JCVI_003_Microviridae_AG0343_putative.VP4
Length=275
Score = 87.4 bits (215), Expect = 3e-21, Method: Compositional matrix adjust.
Identities = 67/240 (28%), Positives = 106/240 (44%), Gaps = 43/240 (18%)
Query 43 VPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYNNEHLPK-------------N 89
VPC KC LC + W+FR L E + A+F+T+TY + LPK +
Sbjct 11 VPCGKCELCRARHVSGWTFRIL-EEMKDAESAHFVTMTYADHELPKIEYYFNDQDYWLLD 69
Query 90 GVFPEEIQLFFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILWNFPDNFESAYS 149
+ ++Q FFKRLR ++ + +RY EYG ++RPHYH I++N S
Sbjct 70 NLDKSDVQKFFKRLRKYQEKTKLP-KIRYYLTGEYGSETERPHYHAIIFN---------S 119
Query 150 RLTLIESCWRRPTGEYHPDGSPVTRSIGFAYCVPVINGGINYVMKYMGKRECAP----EG 205
LI W ++H D + IG + V + Y + YM K P +
Sbjct 120 SEELIIKAW----SKFHLD-TQQREPIGHVHFGKVTEASVRYTLAYMNKPRIIPVDEYDT 174
Query 206 MNSTFMLASRKNGGIGSAY-AEQLRAFYEQQPDTCDMSVINIYTGQPLTTMLPRYYRMKF 264
F L S+ G+G Y +Q++ ++ + + + GQ + +PRYY+ K
Sbjct 175 RQPEFSLMSK---GLGKRYLTDQMKNWH----TSGKRKYVQLPGGQKAS--MPRYYQEKI 225
> Gokush_gi|9634955|ref|NP_054653.1|_nonstructural_protein_[Chlamydia_phage_2]
Length=336
Score = 87.4 bits (215), Expect = 6e-21, Method: Compositional matrix adjust.
Identities = 72/229 (31%), Positives = 114/229 (50%), Gaps = 28/229 (12%)
Query 40 FLIVPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYNNEHLPKNG-VFPEEIQL 98
++++PC +C C + A+ WS+R + E+ + Q F+TLTY + HLP+NG + + +L
Sbjct 71 WVLMPCRRCKFCRVQNAKIWSYRCMHEA-SLYSQNCFLTLTYEDRHLPENGSLVRDHPRL 129
Query 99 FFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILWN--FPDNFESAYSR---LTL 153
F RLR + H +RY EYG +RPHYH++++N FPD + R L +
Sbjct 130 FLMRLREHI----YPHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSKKRGNPLFV 185
Query 154 IESCWRRPTGEYHPDGSPVTRSIGFA--YCVPVINGGINYVMKYMGKRECAPEGMNSTFM 211
E R + GS +S G+ Y + +NG I+ + G+R PE F+
Sbjct 186 SEKLMRLWPFGFSTVGSVTRQSAGYVARYSLKKVNGDIS--QDHYGQR--LPE-----FL 236
Query 212 LASRKNGGIGSAYAEQLRAFYEQQPDTCDMSVINIYTGQPLTTMLPRYY 260
+ S K GIG+ + E+ + Q D V+ G+ T PRYY
Sbjct 237 MCSLK-PGIGADWYEKYKCDVYPQ----DYLVVQD-KGKSFKTRPPRYY 279
> Gokush_gi|17402855|ref|NP_510879.1|_hypothetical_protein_PhiCPG1p9_[Guinea_pig_Chlamydia_phage]
Length=263
Score = 85.1 bits (209), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 72/226 (32%), Positives = 109/226 (48%), Gaps = 28/226 (12%)
Query 43 VPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYNNEHLPKNG-VFPEEIQLFFK 101
+P KC C + A+ WS+R + E+ + Q F+TLTY + HLP+NG + + LF
Sbjct 1 MPWRKCKFCRVQNAKIWSYRCIHEA-SLYSQNCFLTLTYEDRHLPENGSLVRDHPALFLM 59
Query 102 RLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILWN--FPDNFESAYSR---LTLIES 156
RLR ++ H +RY EYG +RPHYH++++N FPD + R L + E
Sbjct 60 RLRKEI----YPHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSKKRGNPLFVSEK 115
Query 157 CWRRPTGEYHPDGSPVTRSIGFA--YCVPVINGGINYVMKYMGKRECAPEGMNSTFMLAS 214
R + GS + +S G+ Y + +NG I+ + G+R P+ F++ S
Sbjct 116 LMRLWPFGFSTVGSVMRQSAGYVARYSLKKVNGDIS--QDHYGQR--LPQ-----FLMCS 166
Query 215 RKNGGIGSAYAEQLRAFYEQQPDTCDMSVINIYTGQPLTTMLPRYY 260
K G Y + R Y Q D V+ G+ TT PRYY
Sbjct 167 LKPGIGADWYEKYKRDVYPQ-----DYLVVQD-KGKSFTTRPPRYY 206
> Gokush_gi|77020121|ref|YP_338244.1|_putative_replication_protein_[Chlamydia_phage_4]
Length=315
Score = 85.1 bits (209), Expect = 3e-20, Method: Compositional matrix adjust.
Identities = 68/227 (30%), Positives = 107/227 (47%), Gaps = 24/227 (11%)
Query 40 FLIVPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFITLTYNNEHLPKNG-VFPEEIQL 98
++++PC +C C + A+ WS+R + E+ + Q F+TLTY ++HLP+NG + L
Sbjct 50 WILMPCRRCKFCRVQNAKIWSYRCMHEA-SLYSQNCFLTLTYEDQHLPENGSLVRNHPTL 108
Query 99 FFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILWN--FPDNFESAYSR---LTL 153
F +RLR + H +RY EYG +RPHYH++++N FPD + R L +
Sbjct 109 FLRRLREHIS----PHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSKKRGNPLFV 164
Query 154 IESCWRRPTGEYHPDGSPVTRSIGFAYCVPVINGGINYVMKYMGKRECAPEGMNSTFMLA 213
E + + GS +S G+ + + + G+R PE F++
Sbjct 165 SEKLMQLWPYGFSTVGSVTRQSAGYVARYSLKKVSRDISQDHYGQR--LPE-----FLMC 217
Query 214 SRKNGGIGSAYAEQLRAFYEQQPDTCDMSVINIYTGQPLTTMLPRYY 260
S K G Y + R Y Q D V+ G+ TT PRYY
Sbjct 218 SLKPGIGADWYEKYKRDVYPQ-----DYLVVQD-KGKSFTTRPPRYY 258
Lambda K H a alpha
0.323 0.137 0.428 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 37528832