bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: all_orgs
14,240,465 sequences; 5,121,972,263 total letters
Query= Contig-23_CDS_annotation_glimmer3.pl_2_7
Length=99
Score E
Sequences producing significant alignments: (Bits) Value
tbi:Tbis_1829 hypothetical protein 34.3 2.8
pca:Pcar_1683 pulM-1; type II secretion system ATPase PulM 33.9 2.9
pbi:103061029 NELFA; negative elongation factor complex member A 34.3 3.0
vni:VIBNI_A1017 putative RNA polymerase sigma-70 factor 33.5 3.8
tru:101076463 negative elongation factor A-like 32.7 7.9
cmk:103183113 nelfa; negative elongation factor complex member A 32.7 8.0
sro:Sros_5868 hypothetical protein 32.7 8.4
oac:Oscil6304_3485 glutamate-1-semialdehyde-2,1-aminomutase 32.7 8.4
ola:101174052 negative elongation factor A-like 32.7 8.5
pfn:HZ99_21715 hypothetical protein 32.0 8.8
mze:101484778 negative elongation factor A-like 32.7 8.8
sus:Acid_4777 histidine kinase 32.7 9.5
xma:102231188 negative elongation factor A-like 32.7 9.6
smo:SELMODRAFT_439046 hypothetical protein 32.7 9.7
dre:559677 nelfa, fi75f03, whsc2, wu:fi75f03; negative elongat... 32.7 9.8
> tbi:Tbis_1829 hypothetical protein
Length=452
Score = 34.3 bits (77), Expect = 2.8, Method: Composition-based stats.
Identities = 22/79 (28%), Positives = 39/79 (49%), Gaps = 6/79 (8%)
Query 14 KNYDPVYSLDFESSKLSKF-LEFSSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLP 72
+ Y Y L S ++++ L + + RG YYL ++ G + + SD+ FE K +P
Sbjct 335 ERYRQKYDLPLSSPRVAQLDLAYHDVHRRRGLYYL--LQRRGAVERVVSDIKIFEAKSIP 392
Query 73 ---ASAQLQGLLLVKVDEE 88
A+L+G + K E+
Sbjct 393 PQTTRARLRGEFIRKAQEK 411
> pca:Pcar_1683 pulM-1; type II secretion system ATPase PulM
Length=295
Score = 33.9 bits (76), Expect = 2.9, Method: Compositional matrix adjust.
Identities = 24/75 (32%), Positives = 40/75 (53%), Gaps = 17/75 (23%)
Query 37 SIFLPRGCYYLPEMELAGFIRSL--SSDVSFFEM-KLLPASAQLQ--------------G 79
++ LP C Y+ M++AGF RS S +V +++ KLLP A L
Sbjct 78 ALSLPDRCGYVMTMDVAGFTRSRKESREVVAWQLRKLLPGIADLHFDYQVLKRTGGGKAH 137
Query 80 LLLVKVDEESLSKYE 94
LL+V +++++L +YE
Sbjct 138 LLVVAMEKQALDRYE 152
> pbi:103061029 NELFA; negative elongation factor complex member
A
Length=529
Score = 34.3 bits (77), Expect = 3.0, Method: Composition-based stats.
Identities = 20/54 (37%), Positives = 27/54 (50%), Gaps = 0/54 (0%)
Query 36 SSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEES 89
SS+ LP C YL + L L+ V F++K P SA L+ LL K E +
Sbjct 144 SSVMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETA 197
> vni:VIBNI_A1017 putative RNA polymerase sigma-70 factor
Length=220
Score = 33.5 bits (75), Expect = 3.8, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 14/100 (14%)
Query 4 FGHDEPDGS---FKNYDPVYS------LDFESSKLSKFLEFSSIFLPRGCYYLPEMELAG 54
FG DEP S F +PV + L+ E +L + + ++F+ R + +E A
Sbjct 100 FGDDEPVSSCPTFSVSEPVMNQNILQLLEKEIDQLP--VMYRTVFVMRSVQGMTSLETAD 157
Query 55 FIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEESLSKYE 94
SL DV+ + + A QLQ L+ ++ ES+S YE
Sbjct 158 ---SLGLDVNVIKTRYRRARLQLQSQLIAHMERESMSLYE 194
> tru:101076463 negative elongation factor A-like
Length=545
Score = 32.7 bits (73), Expect = 7.9, Method: Composition-based stats.
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 0/54 (0%)
Query 36 SSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEES 89
+S LP C YL + L + L+ V F++K P SA L+ LL K E +
Sbjct 133 ASAMLPLECQYLNKSALTTLVGPLTPPVKHFQLKRKPKSATLRAELLQKSTETA 186
> cmk:103183113 nelfa; negative elongation factor complex member
A
Length=552
Score = 32.7 bits (73), Expect = 8.0, Method: Composition-based stats.
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 0/54 (0%)
Query 36 SSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEES 89
+S LP C YL + L + L+ V F++K P SA L+ LL K E +
Sbjct 133 ASAMLPLECQYLNKNALTTLVGPLTPPVKHFQLKRKPKSATLRAELLQKSTETA 186
> sro:Sros_5868 hypothetical protein
Length=452
Score = 32.7 bits (73), Expect = 8.4, Method: Composition-based stats.
Identities = 21/79 (27%), Positives = 40/79 (51%), Gaps = 6/79 (8%)
Query 14 KNYDPVYSLDFESSKLSKF-LEFSSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLP 72
+ Y Y L S ++++ L + + RG +YL ++ G + ++SD+ FE K +P
Sbjct 335 ERYRKKYDLPLSSPRVAQLDLAYHDVHRRRGLFYL--LQKRGAVERVASDLKIFEAKSVP 392
Query 73 ---ASAQLQGLLLVKVDEE 88
A+L+G + K E+
Sbjct 393 PQTTRARLRGEFIRKAQEK 411
> oac:Oscil6304_3485 glutamate-1-semialdehyde-2,1-aminomutase
Length=432
Score = 32.7 bits (73), Expect = 8.4, Method: Composition-based stats.
Identities = 21/53 (40%), Positives = 25/53 (47%), Gaps = 1/53 (2%)
Query 11 GSFKNYDPVYSL-DFESSKLSKFLEFSSIFLPRGCYYLPEMELAGFIRSLSSD 62
G F N PV++ D + S LSKF F L RG Y P AGF +D
Sbjct 363 GLFFNPGPVHNYEDAKKSDLSKFARFHRGMLERGVYLAPSQFEAGFTSLAHTD 415
> ola:101174052 negative elongation factor A-like
Length=546
Score = 32.7 bits (73), Expect = 8.5, Method: Composition-based stats.
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 0/54 (0%)
Query 36 SSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEES 89
+S LP C YL + L + L+ V F++K P SA L+ LL K E +
Sbjct 133 ASAMLPLECQYLNKSALTTLVGPLTPPVKHFQLKRKPKSATLRAELLQKSTETA 186
> pfn:HZ99_21715 hypothetical protein
Length=118
Score = 32.0 bits (71), Expect = 8.8, Method: Compositional matrix adjust.
Identities = 25/99 (25%), Positives = 46/99 (46%), Gaps = 13/99 (13%)
Query 1 LVSFGHDEPDGSF--KNYDPVYSLDFESSKLSKFLEFSSIFLPRGCYYLPEMELAGFIRS 58
L +F + P+G +YDP +D + S+ GCY+ E+ A +R
Sbjct 19 LFAFWNGRPNGDAPASDYDPAAVIDAHQKQASRCC---------GCYF--ELYEAILLRG 67
Query 59 LSSDVSFFEMKLLPASAQLQGLLLVKVDEESLSKYEQEE 97
L +++ E A Q + +K+D+E++++ EQ E
Sbjct 68 LRNELDKLEGADKFAFQQALWVRRIKIDDETIAEAEQAE 106
> mze:101484778 negative elongation factor A-like
Length=547
Score = 32.7 bits (73), Expect = 8.8, Method: Composition-based stats.
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 0/54 (0%)
Query 36 SSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEES 89
+S LP C YL + L + L+ V F++K P SA L+ LL K E +
Sbjct 133 ASAMLPLECQYLNKSALTTLVGPLTPPVKHFQLKRKPKSATLRAELLQKSTETA 186
> sus:Acid_4777 histidine kinase
Length=1358
Score = 32.7 bits (73), Expect = 9.5, Method: Composition-based stats.
Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 8/67 (12%)
Query 27 SKLSKFLEFSSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVD 86
S + + L+ + I RG + +LAG +R + M ++ A AQ++GL L+ D
Sbjct 941 SLVDEVLDVAKIEAGRGVLEIAPYDLAGMVRDV--------MDMMGARAQMKGLALLHAD 992
Query 87 EESLSKY 93
L +Y
Sbjct 993 SSGLPRY 999
> xma:102231188 negative elongation factor A-like
Length=547
Score = 32.7 bits (73), Expect = 9.6, Method: Composition-based stats.
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 0/54 (0%)
Query 36 SSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEES 89
+S LP C YL + L + L+ V F++K P SA L+ LL K E +
Sbjct 133 ASAMLPLECQYLNKNALTTLVGPLTPPVKHFQLKRKPKSATLRAELLQKSTETA 186
> smo:SELMODRAFT_439046 hypothetical protein
Length=388
Score = 32.7 bits (73), Expect = 9.7, Method: Composition-based stats.
Identities = 16/54 (30%), Positives = 31/54 (57%), Gaps = 11/54 (20%)
Query 39 FLPRGCYYLPEMELA-------GFIRSLSS----DVSFFEMKLLPASAQLQGLL 81
F+P CY++P +LA GF++SL + D+S + + + P + +QG++
Sbjct 75 FVPGSCYWVPSKDLALLPGDAYGFVKSLGASEACDMSLWILMINPETLNVQGVV 128
> dre:559677 nelfa, fi75f03, whsc2, wu:fi75f03; negative elongation
factor complex member A
Length=542
Score = 32.7 bits (73), Expect = 9.8, Method: Composition-based stats.
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 0/54 (0%)
Query 36 SSIFLPRGCYYLPEMELAGFIRSLSSDVSFFEMKLLPASAQLQGLLLVKVDEES 89
+S LP C YL + L + L+ + F++K P SA L+ LL K E +
Sbjct 133 ASTMLPLECQYLNKSALTTLVGPLTPPIKHFQLKRKPKSATLRAELLQKSTETA 186
Lambda K H a alpha
0.316 0.137 0.382 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 128762239933