bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-18_CDS_annotation_glimmer3.pl_2_8
Length=222
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_C_016_Microviridae_AG0273_putative.VP4 436 1e-156
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 96.3 3e-25
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 93.2 3e-24
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 89.0 8e-23
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 89.0 1e-22
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 88.6 1e-22
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 87.8 2e-22
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 88.2 2e-22
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 87.4 3e-22
Pichovirinae_66_Microbialites_001_Microviridae_AG0131_putative.VP4 83.6 5e-21
> Alpavirinae_Human_feces_C_016_Microviridae_AG0273_putative.VP4
Length=304
Score = 436 bits (1120), Expect = 1e-156, Method: Compositional matrix adjust.
Identities = 205/222 (92%), Positives = 213/222 (96%), Gaps = 0/222 (0%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFGTLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLASR 60
LFLDRLRKDY KQIRHWFVCEFGTL RPHYHGILF+VPQ LIDGYSPDVPGHHPLLASR
Sbjct 83 LFLDRLRKDYGKQIRHWFVCEFGTLRCRPHYHGILFNVPQALIDGYSPDVPGHHPLLASR 142
Query 61 WKYGFVFVGYVSEETCSYITKYVTKSINGDKVRPRIISSFGIGSNYLDTEEAVLHKLGNQ 120
WKYGFVFVGYVSEETCSYITKYVTKSINGDKVRPR+ISSFGIGSNYLDTEE+ LHKLGNQ
Sbjct 143 WKYGFVFVGYVSEETCSYITKYVTKSINGDKVRPRVISSFGIGSNYLDTEESSLHKLGNQ 202
Query 121 HYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRFINPPVEFSWQGQKFSSKLERDEM 180
YQPFMVLNGFQQAMPRYYYNK+FSDVDKQN+V+DRFINPPVEFSWQGQKF SKLERDEM
Sbjct 203 RYQPFMVLNGFQQAMPRYYYNKLFSDVDKQNMVIDRFINPPVEFSWQGQKFGSKLERDEM 262
Query 181 RRSTLNQNITSGLTPALPLPHTERVSSFNRFKKNMDKNKEFK 222
RRSTLNQNI SGLTP LPLPHTERVSSF+RFK+NMDKNKEFK
Sbjct 263 RRSTLNQNIASGLTPVLPLPHTERVSSFDRFKENMDKNKEFK 304
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 96.3 bits (238), Expect = 3e-25, Method: Compositional matrix adjust.
Identities = 55/159 (35%), Positives = 84/159 (53%), Gaps = 17/159 (11%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFGTLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLASR 60
LFL+R RKD K IRHW V E G R H HGI F L+
Sbjct 102 LFLERTRKDTGKSIRHWCVTELGEKNNRIHLHGIFFGQKSA-------------ELIRKH 148
Query 61 WKYGFVFV-GYVSEETCSYITKYVTK-SINGDKVRPRIISSFGIGSNYLDTEEAVLHKLG 118
W YGF+F+ GY + ++ +YITKY+ K I + + +++S GIG Y+D + + K
Sbjct 149 WNYGFIFIGGYCNSKSINYITKYMLKVDIKHPEFKQIVLASSGIGKGYIDRLDYLWQKQN 208
Query 119 --NQHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLD 155
N + + NG + AMP+YY NKIF++ +++ + ++
Sbjct 209 YKNINVATYTFRNGTKMAMPKYYKNKIFTEKEREKMWIN 247
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 93.2 bits (230), Expect = 3e-24, Method: Compositional matrix adjust.
Identities = 55/161 (34%), Positives = 90/161 (56%), Gaps = 17/161 (11%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLAS 59
L L+R+RK K I+HWF+ E G R H HGI++ + G L++
Sbjct 100 LMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGI-------------GTDQLISE 146
Query 60 RWKYGFVFVG-YVSEETCSYITKYVTK-SINGDKVRPRIISSFGIGSNYLDTEEAVLHK- 116
+W YGFV+ G +V+E T +YITKY+TK I+ +++ S GIG+ Y E+A HK
Sbjct 147 KWNYGFVYTGNFVNEATINYITKYMTKVDIDHPDFVGQVLCSKGIGAGYTKREDANNHKY 206
Query 117 LGNQHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 157
+ + + + NG + +P YY N++FS+ +++ + LD+
Sbjct 207 TKGKTNETYRLRNGAKINLPIYYRNQLFSEEEREMLFLDKI 247
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 89.0 bits (219), Expect = 8e-23, Method: Compositional matrix adjust.
Identities = 53/161 (33%), Positives = 87/161 (54%), Gaps = 17/161 (11%)
Query 1 LFLDRLRKDYSKQIRHWFVCEF-GTLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLAS 59
LFL+R+RK K ++HW E GT R H HG+++ L S
Sbjct 100 LFLERVRKKTKKSLKHWLTTERGGTNTERYHLHGLIWG-------------ENTEALTKS 146
Query 60 RWKYGFVFVG-YVSEETCSYITKYVTKSINGDK-VRPRIISSFGIGSNYLDTEEAVLHKL 117
W+YGFVF+G +V+E T +YITKY+TK+ K P + S GIG YL ++ L++
Sbjct 147 LWQYGFVFIGTFVNECTVNYITKYITKTDKKHKDFEPITLCSAGIGKGYLSRSDSELNRF 206
Query 118 -GNQHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 157
+ + + + NG + +P YY NK+++D +++ + L++
Sbjct 207 REGKTTETYRLRNGTKLNLPIYYRNKLYTDEEREKLFLEKI 247
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 89.0 bits (219), Expect = 1e-22, Method: Compositional matrix adjust.
Identities = 53/164 (32%), Positives = 88/164 (54%), Gaps = 23/164 (14%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLAS 59
L L+R+RK K IRHWF+ E G R H HGI++ + G L+
Sbjct 100 LMLERIRKKIGKSIRHWFITELGHEKTERLHLHGIVWGI-------------GTDQLIRE 146
Query 60 RWKYGFVFVG-YVSEETCSYITKYVTKSINGDKVRP----RIISSFGIGSNYLDTEEAVL 114
+W YG + G +V+E+T +YITKY+TK D+ P +++ S GIG+ Y +A
Sbjct 147 KWNYGITYTGNFVNEKTINYITKYMTKI---DEEHPNFVGKVLCSKGIGAGYTKRPDAAK 203
Query 115 HKLGN-QHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 157
HK + + + + NG + +P YY NK+F++ +++ + +D+
Sbjct 204 HKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKERELLFIDKI 247
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 88.6 bits (218), Expect = 1e-22, Method: Compositional matrix adjust.
Identities = 52/164 (32%), Positives = 88/164 (54%), Gaps = 23/164 (14%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLAS 59
L L+R+RK K I+HWF+ E G R H HGI++ + G L+
Sbjct 100 LMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGI-------------GTDQLIEE 146
Query 60 RWKYGFVFVG-YVSEETCSYITKYVTKSINGDKVRP----RIISSFGIGSNYLDTEEAVL 114
+W YG + G YV+E+T +Y+TKY+TK D+ P +++ S GIG+ Y +A
Sbjct 147 KWNYGITYTGNYVNEKTINYVTKYMTKI---DEKHPDFVGKVLCSRGIGAGYTKRPDAAK 203
Query 115 HKLGN-QHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 157
HK + + + + NG + +P YY NK+F++ +++ + +D+
Sbjct 204 HKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKERELLFIDKI 247
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 87.8 bits (216), Expect = 2e-22, Method: Compositional matrix adjust.
Identities = 52/161 (32%), Positives = 87/161 (54%), Gaps = 17/161 (11%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLAS 59
L L+R+RK K I+HWF+ E G R H HGI++ + G L+ +
Sbjct 59 LCLERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGI-------------GTDKLITN 105
Query 60 RWKYGFVFVG-YVSEETCSYITKYVTKSINGDK-VRPRIISSFGIGSNYLDTEEAVLHKL 117
+W YG F G +V+E+T YITKY+TK K +++ S GIG+ Y+ ++A H
Sbjct 106 KWNYGITFTGFFVNEKTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTY 165
Query 118 G-NQHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 157
+ + + + NG + +P YY N++F++ +K+ + LD+
Sbjct 166 KPGETIETYRLRNGSKINLPIYYRNQLFTEEEKEALFLDKI 206
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 88.2 bits (217), Expect = 2e-22, Method: Compositional matrix adjust.
Identities = 52/161 (32%), Positives = 87/161 (54%), Gaps = 17/161 (11%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLAS 59
L L+R+RK K I+HWF+ E G R H HGI++ + G L+ S
Sbjct 100 LCLERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGI-------------GTDKLITS 146
Query 60 RWKYGFVFVG-YVSEETCSYITKYVTKSINGDK-VRPRIISSFGIGSNYLDTEEAVLHKL 117
+W YG F G +V+E+T YITKY+TK K +++ S GIG+ Y+ ++A H
Sbjct 147 KWNYGITFTGFFVNEKTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTY 206
Query 118 G-NQHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 157
+ + + + NG + +P YY N++F++ +++ + LD+
Sbjct 207 KRGETIETYRLRNGSKINLPIYYRNQLFTEEEREALFLDKI 247
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 87.4 bits (215), Expect = 3e-22, Method: Compositional matrix adjust.
Identities = 49/156 (31%), Positives = 87/156 (56%), Gaps = 19/156 (12%)
Query 2 FLDRLRKDYSKQIRHWFVCEFGTLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLASRW 61
+L+R+RK K IRHW V E G YGR H HGI++ P+ I+ +W
Sbjct 107 WLERIRKKTKKSIRHWMVTEKGEDYGRIHLHGIVW-CPKERIE---------------QW 150
Query 62 KYGFVFVG-YVSEETCSYITKYVTKSING-DKVRPRIISSFGIGSNYLDTEEAVLHKL-G 118
YG+ ++G YV+E T +Y+TKY+ K R +++ S GIG Y + A ++ G
Sbjct 151 GYGYTYIGDYVNETTIAYVTKYMLKICEKWPDFRGKVMCSAGIGCRYETSYNAKRNRYRG 210
Query 119 NQHYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVL 154
+ + + + NG + +P+YY++KI+++ +++ + +
Sbjct 211 KETKETYKLENGRELPLPKYYHDKIYTEEEREKLWI 246
> Pichovirinae_66_Microbialites_001_Microviridae_AG0131_putative.VP4
Length=291
Score = 83.6 bits (205), Expect = 5e-21, Method: Compositional matrix adjust.
Identities = 59/166 (36%), Positives = 88/166 (53%), Gaps = 21/166 (13%)
Query 1 LFLDRLRKDYSKQIRHWFVCEFGTLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLASR 60
LF+ RLRK + Q++++ V E+GT RPHYH ILF V D+ H
Sbjct 94 LFMKRLRKYETAQLKYYAVGEYGTNNKRPHYHMILFGV---------SDIENIH----KA 140
Query 61 WKYGFVFVGYVSEETCSYITKYVTKS------INGDKVRPRIISSFGIGSNYLDTEEAVL 114
W YG V+VG V+ ++ +Y KY+ KS D++ + S G+G NYL T E V
Sbjct 141 WNYGSVWVGTVTGDSIAYTMKYIDKSRWRPDHWRDDRIPEFPLMSKGLGENYL-THEVVK 199
Query 115 HKLGNQHYQPFMVLNGFQQAMPRYYYNKIFSDVD-KQNIVLDRFIN 159
+ + G + A+PRYY NKI+SD++ KQ +V+ + I+
Sbjct 200 YHKSDVSRMYATKEGGHKIALPRYYRNKIYSDLEKKQQLVIAQSIS 245
Lambda K H a alpha
0.322 0.140 0.435 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 17280362