bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Microviridae_proteins.fasta 575 sequences; 147,441 total letters Query= Contig-20_CDS_annotation_glimmer3.pl_2_2 Length=135 Score E Sequences producing significant alignments: (Bits) Value Gokush_Human_feces_D_014_Microviridae_AG030_putative.VP4 21.9 2.4 Gokush_Human_feces_C_031_Microviridae_AG0433_putative.VP1 20.8 6.2 Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4 20.8 7.1 Alpavirinae_Human_feces_A_048_Microviridae_AG087_putative.VP1 20.4 9.6 > Gokush_Human_feces_D_014_Microviridae_AG030_putative.VP4 Length=348 Score = 21.9 bits (45), Expect = 2.4, Method: Compositional matrix adjust. Identities = 12/50 (24%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Query 69 LDYSSIFLPKGCYYLSDMELAGFIKALSFGASTFEMKLLPASAQMQGMIL 118 L+YS + + CY + M A + L++ + L+PA + G ++ Sbjct 70 LEYSKQWADR-CYLEAKMWKANYWLTLTYDEEHIQHLLVPAVDKKTGEVI 118 > Gokush_Human_feces_C_031_Microviridae_AG0433_putative.VP1 Length=529 Score = 20.8 bits (42), Expect = 6.2, Method: Composition-based stats. Identities = 11/20 (55%), Positives = 12/20 (60%), Gaps = 2/20 (10%) Query 33 NGGYLVSFSQEE--PNGSFK 50 N GYLV F +E P SFK Sbjct 36 NSGYLVPFYVDEVLPGDSFK 55 > Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4 Length=546 Score = 20.8 bits (42), Expect = 7.1, Method: Compositional matrix adjust. Identities = 16/43 (37%), Positives = 21/43 (49%), Gaps = 12/43 (28%) Query 51 SYDPSYSLDFEASKLSTYLDYSSIFLP------KGCY-YLSDM 86 SYD + ++F+A K DY F KGCY +L DM Sbjct 114 SYDDDFRVEFKADK-----DYIENFKKQANLDVKGCYPHLKDM 151 > Alpavirinae_Human_feces_A_048_Microviridae_AG087_putative.VP1 Length=650 Score = 20.4 bits (41), Expect = 9.6, Method: Composition-based stats. Identities = 8/22 (36%), Positives = 11/22 (50%), Gaps = 0/22 (0%) Query 37 LVSFSQEEPNGSFKSYDPSYSL 58 L SF++ PNG + Y L Sbjct 332 LKSFAERLPNGKLNTSSVQYGL 353 Lambda K H a alpha 0.312 0.130 0.355 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 8470575