bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-22_CDS_annotation_glimmer3.pl_2_3
Length=69
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_A_034_Microviridae_AG0104_hypothetical.... 113 1e-35
Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1 22.7 0.30
Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1 22.3 0.46
Gokush_Bourget_245_Microviridae_AG080_putative.VP4 21.9 0.64
Gokush_Bourget_259_Microviridae_AG074_putative.VP4 20.0 3.2
Pichovirinae_Pavin_723_Microviridae_AG0327_putative.VP1 18.9 7.7
> Alpavirinae_Human_feces_A_034_Microviridae_AG0104_hypothetical.protein
Length=69
Score = 113 bits (282), Expect = 1e-35, Method: Compositional matrix adjust.
Identities = 51/69 (74%), Positives = 61/69 (88%), Gaps = 0/69 (0%)
Query 1 MSKVDSFPSIHNSVVDGIPENYVHYYKKYLYEIEFFRFKKGVSVDSRYCDVPVAEKEKQL 60
M VDSFPSI+N +VDGIPENYVHYYKKYLYE+EF R K+ +S+ R+CD+PVAEKEKQL
Sbjct 1 MLNVDSFPSIYNDIVDGIPENYVHYYKKYLYEVEFIRLKESISIPLRHCDIPVAEKEKQL 60
Query 61 IINFKFEEK 69
+INFKF+EK
Sbjct 61 VINFKFDEK 69
> Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1
Length=607
Score = 22.7 bits (47), Expect = 0.30, Method: Composition-based stats.
Identities = 15/57 (26%), Positives = 30/57 (53%), Gaps = 5/57 (9%)
Query 4 VDSFPSIHNSVVDGIPENYVHYYKK-YLYEIEFFRFKK----GVSVDSRYCDVPVAE 55
VD F + +V +PE Y+K ++++++ + K GV +S++ DV V +
Sbjct 224 VDYFTGVSPLLVSSLPEASNAYWKSPTMFDLKYCNWNKDMLMGVLPNSQFGDVAVLD 280
> Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1
Length=614
Score = 22.3 bits (46), Expect = 0.46, Method: Composition-based stats.
Identities = 15/57 (26%), Positives = 29/57 (51%), Gaps = 5/57 (9%)
Query 4 VDSFPSIHNSVVDGIPENYVHYYKK-YLYEIEFFRFKK----GVSVDSRYCDVPVAE 55
VD F S+V +P Y+K ++++++ + K GV +S++ DV V +
Sbjct 233 VDYFSGSSPSIVSDLPAASSAYWKSDTMFDLKYCNWNKDMLMGVLPNSQFGDVAVLD 289
> Gokush_Bourget_245_Microviridae_AG080_putative.VP4
Length=282
Score = 21.9 bits (45), Expect = 0.64, Method: Compositional matrix adjust.
Identities = 17/65 (26%), Positives = 25/65 (38%), Gaps = 10/65 (15%)
Query 5 DSFPSIHNSVVDGIPENYVHYYKKYLYEIEF-----FRFKKGVSVD-----SRYCDVPVA 54
D FPS + G YYK L E+ +F+ D RY ++PV
Sbjct 204 DVFPSASVVTIQGTRAPVPRYYKTLLKELGHDLSLDMQFRSSARADLEVEQKRYENLPVR 263
Query 55 EKEKQ 59
+ +Q
Sbjct 264 KIARQ 268
> Gokush_Bourget_259_Microviridae_AG074_putative.VP4
Length=286
Score = 20.0 bits (40), Expect = 3.2, Method: Compositional matrix adjust.
Identities = 10/29 (34%), Positives = 12/29 (41%), Gaps = 0/29 (0%)
Query 5 DSFPSIHNSVVDGIPENYVHYYKKYLYEI 33
D FPS + G YYK L E+
Sbjct 217 DVFPSASVVTIQGTRAPVPRYYKTLLKEL 245
> Pichovirinae_Pavin_723_Microviridae_AG0327_putative.VP1
Length=508
Score = 18.9 bits (37), Expect = 7.7, Method: Composition-based stats.
Identities = 8/26 (31%), Positives = 15/26 (58%), Gaps = 5/26 (19%)
Query 39 KKGVSVDSRYCDVPVAEKEKQLIINF 64
+KG +VD +P+ +K + +NF
Sbjct 200 QKGAAVD-----IPIGTIDKDVAVNF 220
Lambda K H a alpha
0.321 0.139 0.409 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 3608789