bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-39_CDS_annotation_glimmer3.pl_2_1
Length=225
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_gut_30_017_Microviridae_AG0205_putative.VP1 436 6e-152
Pichovirinae_Pavin_723_Microviridae_AG0327_putative.VP1 49.3 8e-09
Alpavirinae_Human_feces_C_016_Microviridae_AG0274_putative.VP1 44.3 4e-07
Alpavirinae_Human_gut_30_040_Microviridae_AG0134_putative.VP1 43.5 7e-07
Alpavirinae_Human_feces_B_007_Microviridae_AG068_putative.VP1 40.4 6e-06
Pichovirinae_Pavin_279_Microviridae_AG0271_putative.VP1 38.5 3e-05
Alpavirinae_Human_feces_C_010_Microviridae_AG0199_putative.VP1 37.7 6e-05
Pichovirinae_JCVI_003_Microviridae_AG0344_putative.VP1 35.0 4e-04
Gokush_Bourget_259_Microviridae_AG073_putative.VP1 34.7 5e-04
Alpavirinae_Human_gut_21_005_Microviridae_AG016_putative.VP1 34.3 7e-04
> Alpavirinae_Human_gut_30_017_Microviridae_AG0205_putative.VP1
Length=647
Score = 436 bits (1121), Expect = 6e-152, Method: Compositional matrix adjust.
Identities = 208/225 (92%), Positives = 220/225 (98%), Gaps = 0/225 (0%)
Query 1 VDRPKLLFSSSVMVNSQVVLNQAGQSGFEGGESAALGQMGGSISFNTVLGREQTYYFKEP 60
VDRPKLLFSSSVMVNSQVV+NQAGQSGF GGE+AALGQMGGSI+FNTVLGREQTYYFKEP
Sbjct 423 VDRPKLLFSSSVMVNSQVVMNQAGQSGFAGGEAAALGQMGGSIAFNTVLGREQTYYFKEP 482
Query 61 GYIFDMMTIRPVYFWTGIRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLGYGWKADTVSSL 120
GYIFDM+TIRPVYFWTGIRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLG+GWK+D VSS+
Sbjct 483 GYIFDMLTIRPVYFWTGIRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLGFGWKSDAVSSV 542
Query 121 SVAKEPCYNEFRSSYDEVLGSLQATLTPKASTPLQSYWVQQRDFYLIGLSSNPNEVSPSM 180
SVAKEPCYNEFRSSYDEVLGSLQ+TLTPKAS PLQSYWVQQRDFYLIGLSS+ NE+SPSM
Sbjct 543 SVAKEPCYNEFRSSYDEVLGSLQSTLTPKASVPLQSYWVQQRDFYLIGLSSDLNEISPSM 602
Query 181 LFTNLNTVNNPFASDMEDNFFVNMSYKVVVKNLINKSFATRLSSR 225
LFTNL TVNNPF+SDMEDNFFVNMSYKVVVKNL+NKSFATRLSSR
Sbjct 603 LFTNLATVNNPFSSDMEDNFFVNMSYKVVVKNLVNKSFATRLSSR 647
> Pichovirinae_Pavin_723_Microviridae_AG0327_putative.VP1
Length=508
Score = 49.3 bits (116), Expect = 8e-09, Method: Compositional matrix adjust.
Identities = 45/165 (27%), Positives = 69/165 (42%), Gaps = 19/165 (12%)
Query 1 VDRPKLLFSSSVMVNSQVVLNQAGQSGFEGGESAALGQMGGSISFNTVLGREQTYYFKEP 60
+ RP+ + V VLN GQ G G M G + G+ +YY +E
Sbjct 309 LQRPEYITGVKSPVVVSEVLNTTGQDG-----GLPQGNMAGH-GISVTSGKSGSYYCEEH 362
Query 61 GYIFDMMTIRP-VYFWTGIRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLGYGWKADTVSS 119
GYI +M++ P + GI +L+ DYF P + +IG Q+V L Y + A+ +
Sbjct 363 GYIIGIMSVMPKTAYQQGIPRTFLKTDSLDYFWPTFANIGEQEVAKQEL-YAYTANANDT 421
Query 120 LSVAKEPCYNEFRSSYDEVLGSLQATLTPKASTPLQSYWVQQRDF 164
P Y E++ V G + +L +YW R F
Sbjct 422 FGYV--PRYAEYKYMPSRVAGEFRTSL---------NYWHLGRIF 455
> Alpavirinae_Human_feces_C_016_Microviridae_AG0274_putative.VP1
Length=641
Score = 44.3 bits (103), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 53/193 (27%), Positives = 74/193 (38%), Gaps = 42/193 (22%)
Query 1 VDRPKLLFSSSVMVNSQVVLNQAGQSGFEGGESAALGQMGGSIS----FNTVLGREQTYY 56
V++P L +N V +A +G GE A LGQ+ + F+ G + YY
Sbjct 397 VNKPDFLGVWQASINPSNV--RAMANGSASGEDANLGQLAACVDRYCDFSDHSGID--YY 452
Query 57 FKEPG--YIFDMMTIRPVYFWTGIRPDYLEYRGPDYFNPIYNDIGYQDVPLWRL------ 108
KEPG + M+ P Y G+ PD D FNP N IG+Q VP R
Sbjct 453 AKEPGTFMLITMLVPEPAYS-QGLHPDLASISFGDDFNPELNGIGFQLVPRHRFSMMPRG 511
Query 109 -----------------GYGWKADTVSSLSVAKEPCYNEFRSSYDEVLGSLQATLTPKAS 151
G G D +++SV +E ++ R+ Y + G A
Sbjct 512 FNLTGLDQQTSPWFGNAGTGVVIDP-NTVSVGEEVAWSWLRTDYSRLHGDF-------AQ 563
Query 152 TPLQSYWVQQRDF 164
YWV R F
Sbjct 564 NGNYQYWVLTRRF 576
> Alpavirinae_Human_gut_30_040_Microviridae_AG0134_putative.VP1
Length=679
Score = 43.5 bits (101), Expect = 7e-07, Method: Compositional matrix adjust.
Identities = 40/148 (27%), Positives = 67/148 (45%), Gaps = 18/148 (12%)
Query 1 VDRPKLLFSSSVMVNSQVVLNQAGQS----GFEGGESAALGQMGGSISFNTVLGREQTYY 56
++ P L SS+ + Q V+N +G G G A GG+I F
Sbjct 478 IETPLYLGGSSLEIEFQEVINNSGTEDQPLGALAGRGVATNHKGGNIVFKA--------- 528
Query 57 FKEPGYIFDMMTIRP-VYFWTGIRPD-YLEYRGPDYFNPIYNDIGYQDVPLWRLGYGWKA 114
EPGYIF + +I P V ++ G D YLE D P + IG+QD L
Sbjct 529 -DEPGYIFCITSITPRVDYFQGNEWDLYLESL-DDLHKPQLDGIGFQDRLYRHLNSSCVR 586
Query 115 DTVSSLSVAKEPCYNEFRSSYDEVLGSL 142
+ + ++S+ K+P + ++ ++ ++ G+
Sbjct 587 EDL-NISIGKQPAWVQYMTNVNKTYGNF 613
> Alpavirinae_Human_feces_B_007_Microviridae_AG068_putative.VP1
Length=780
Score = 40.4 bits (93), Expect = 6e-06, Method: Compositional matrix adjust.
Identities = 45/217 (21%), Positives = 83/217 (38%), Gaps = 24/217 (11%)
Query 14 VNSQVVLNQAGQSGFEGGESAALGQMGGSISFNTVLGREQ---TYYFKEPGYIFDMMTIR 70
+ +V+N Q+ + +G +G T G + + E + +M +
Sbjct 579 ITRDIVVNPITQTVETTDTGSYVGSLGSQAGLATCFGNSDGSISVFCDEESIVMGIMYVM 638
Query 71 PVYFWTGIRPDYLEYRGP-DYFNPIYNDIGYQDVPLWRLGYGWKADTVSSLSVA---KEP 126
P+ + I P +L YR D FNP ++ IGYQ + L L ++ L+ + P
Sbjct 639 PMPVYDSILPKWLTYRERLDSFNPEFDHIGYQPIYLKELAAIQAFESGKDLNTVFGYQRP 698
Query 127 CYNEFRSSYDEVLGSLQATLTPKASTPLQSYWVQQRDFYLIGLSSNPNEVSPSMLFTNLN 186
Y E+ D G ++L R+F + N E+ S
Sbjct 699 WY-EYVQKVDRAHGLFLSSL---------------RNFIMFRSFENAPELGKSFTVMQPG 742
Query 187 TVNNPFA-SDMEDNFFVNMSYKVVVKNLINKSFATRL 222
+VNN F+ +++ D + + + I++ RL
Sbjct 743 SVNNVFSVTEVSDKILGQIHFDCTAQLPISRVVVPRL 779
> Pichovirinae_Pavin_279_Microviridae_AG0271_putative.VP1
Length=503
Score = 38.5 bits (88), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 52/198 (26%), Positives = 83/198 (42%), Gaps = 27/198 (14%)
Query 19 VLNQAGQSGFEGGESAALGQMGGSISFNTVLGREQTYYFKEPGYIFDMMTIRPV-YFWTG 77
V+ Q S +G E G + S + G Y +E GYI +M+I P + G
Sbjct 317 VVVQEITSTVKGIEEPQGNPTGKATSLQS--GHYGNYSVEEHGYIIGIMSIMPKPAYQQG 374
Query 78 IRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLGYGWKADTVSSLSVAKEPCYNEFRSSYDE 137
I YL+ D++ P + +IG Q+V L YG+ + P Y E++ +
Sbjct 375 IPKSYLKTDVLDFYWPSFANIGEQEV-LQEEIYGYTPTPNAVFGYI--PRYAEYKYLQNR 431
Query 138 VLGSLQATLTPKASTPLQSYWVQQRDFYLI-GLSSNPNEVSP---SMLFTNLNTVNNPFA 193
V G + TL +W R F I LS + EVSP + +F N
Sbjct 432 VCGLFRTTL---------DFWHLGRIFANIPTLSQSFIEVSPANQTRIFAN--------Q 474
Query 194 SDMEDNFFVNMSYKVVVK 211
D +DN ++++ K+ +
Sbjct 475 EDYDDNIYIHVLNKMTAR 492
> Alpavirinae_Human_feces_C_010_Microviridae_AG0199_putative.VP1
Length=731
Score = 37.7 bits (86), Expect = 6e-05, Method: Compositional matrix adjust.
Identities = 46/219 (21%), Positives = 87/219 (40%), Gaps = 25/219 (11%)
Query 11 SVMVNSQVVLNQAGQSGFEGGESAALGQMGGSISFNTVLGREQTYYFKEPGYIFDMMTIR 70
S+ Q V Q+G S G + ALG G + E YI ++T+
Sbjct 530 SMRTVEQTVDQQSGSS--RGQYAEALGSKTGIAGVYGSTSNNIEVFCDEESYIIGLLTVT 587
Query 71 PVYFWTGIRPDYLEYRG-PDYFNPIYNDIGYQ-----DVPLWRLGYGWKADTVSSLSVAK 124
PV +T + P Y G D++ P ++ IG+Q ++ + G A+ ++ +
Sbjct 588 PVPIYTQLLPKDFTYNGLLDHYQPEFDRIGFQPITYKEICPMNIVAGDSANQLNKTFGYQ 647
Query 125 EPCYNEFRSSYDEVLGSLQATLTPKASTPLQSYWVQQRDFYLIGLSSNPNEVSPSMLFTN 184
P Y E+ + YD G + + ++F + + S ++ L +
Sbjct 648 RPWY-EYVAKYDSAHGLFRTNM---------------KNFIMSRVFSGLPQLGQQFLLVD 691
Query 185 LNTVNNPFA-SDMEDNFFVNMSYKVVVKNLINKSFATRL 222
+TVN F+ ++ D F + + + I++ RL
Sbjct 692 PDTVNQVFSVTEYTDKIFGYVKFNATARLPISRVAIPRL 730
> Pichovirinae_JCVI_003_Microviridae_AG0344_putative.VP1
Length=505
Score = 35.0 bits (79), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 47/214 (22%), Positives = 87/214 (41%), Gaps = 28/214 (13%)
Query 1 VDRPKLLFSSSVMVNSQVVLNQAGQS-GFEGGESAALGQMGGSISFNTVLGREQTYYFKE 59
+ RP+ + + V +LN G++ G G + G G+ G YY +E
Sbjct 306 LQRPEYITGTKQPVIISEILNTTGETAGLPQGNMSGHGVAVGT-------GNVGKYYCEE 358
Query 60 PGYIFDMMTIRP-VYFWTGIRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLGYGWKADTVS 118
G+I ++++ P + GI YL D++ P + IG Q + + + D
Sbjct 359 HGFIIGILSVTPNTAYQQGIPKHYLRTDKYDFYWPQFAHIGEQPIVNNEI---YAYDPTG 415
Query 119 SLSVAKEPCYNEFRSSYDEVLGSLQATLTPKASTPLQSYWVQQRDFYLIGLSSNPNEVSP 178
+ P Y E++ V G +++L W R F SS P +S
Sbjct 416 DETFGYTPRYAEYKYMPSRVAGEFRSSL---------DEWHAARIF-----SSLPT-LSQ 460
Query 179 SMLFTNLNTVNNPFA-SDMEDNFFVNMSYKVVVK 211
+ + + + FA +D +DN ++++ KV +
Sbjct 461 DFIEVDADDFDRIFAVTDGDDNLYMHVLNKVKAR 494
> Gokush_Bourget_259_Microviridae_AG073_putative.VP1
Length=540
Score = 34.7 bits (78), Expect = 5e-04, Method: Compositional matrix adjust.
Identities = 47/199 (24%), Positives = 83/199 (42%), Gaps = 34/199 (17%)
Query 1 VDRPKLLFSSSVMVN-SQVVLNQAGQSGFEGGESAALGQMGGSISFNTVLGREQ--TYYF 57
+ RP+ L S +N S + N A G S + +G +F T LG+E T F
Sbjct 327 LQRPEYLGGGSTNINISPIAQNSAT------GLSGSTTPIGNLAAFGTFLGKEHGFTQSF 380
Query 58 KEPGYIFDMMTIRP-VYFWTGIRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLGYGWKADT 116
E GY+ ++++R + + G+R + DY+ P + +G Q V L + Y T
Sbjct 381 VEHGYVIGLVSVRADLTYQQGLRRHWSRSTRYDYYFPAFATLGEQSV-LNKEIYVTGNTT 439
Query 117 VSSLSVAKEPCYNEFRSSYDEVLGSLQATLTPKASTPLQSYWVQQRDFYLIGLSSNPNEV 176
+ + + E+R + E+ G ++T +S + + Q+
Sbjct 440 QDNSVFGYQERWAEYRYNPSEITGLFKST----SSGTIDPWHYAQK-------------- 481
Query 177 SPSMLFTNLNTVNNPFASD 195
FT+L T+N+ F D
Sbjct 482 -----FTSLPTLNSTFIQD 495
> Alpavirinae_Human_gut_21_005_Microviridae_AG016_putative.VP1
Length=742
Score = 34.3 bits (77), Expect = 7e-04, Method: Compositional matrix adjust.
Identities = 24/94 (26%), Positives = 41/94 (44%), Gaps = 3/94 (3%)
Query 11 SVMVNSQVVLNQAGQSGFEGGESAALGQMGGSISFNTVLGREQTYYFKEPGYIFDMMTIR 70
S+ Q V Q+ S +G + ALG G + E YI ++T+
Sbjct 542 SMRTVEQTVDQQSASS--QGQYAEALGSKTGIAGVYGSTSNNIEVFCDEESYIIGLLTVT 599
Query 71 PVYFWTGIRPDYLEYRG-PDYFNPIYNDIGYQDV 103
PV ++ + P Y G D++ P ++ IG+Q +
Sbjct 600 PVPIYSQLLPKDFTYNGLLDHYQPEFDRIGFQPI 633
Lambda K H a alpha
0.318 0.135 0.406 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 17610560