bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-18_CDS_annotation_glimmer3.pl_2_6
Length=303
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 214 2e-68
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 213 7e-68
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 211 3e-67
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 210 7e-67
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 206 2e-65
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 192 3e-60
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 186 6e-58
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 184 1e-56
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 177 9e-55
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 169 1e-50
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 214 bits (545), Expect = 2e-68, Method: Compositional matrix adjust.
Identities = 112/254 (44%), Positives = 161/254 (63%), Gaps = 6/254 (2%)
Query 1 VCLYPSIIENPKYAKSNKNG---KRARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+CLYP +I+N +Y + KNG + D RLR+I CG C ECR+ K +W VR+ EE+
Sbjct 1 MCLYPKLIKNRRYVPNKKNGGVPPQCPDERLRYITAACGKCMECRQQKQRQWLVRMSEEL 60
Query 58 KSYPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLIT 115
+ P N F TLT ++S +L Y+ + N+ KAI L +R K+ +KHW IT
Sbjct 61 RQEP-NAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKSIKHWFIT 119
Query 116 EMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANP 175
E+GH+ T+R+HLHGI+W T++ +W YG F G+ V+EKTI YI KY+TK DE +
Sbjct 120 ELGHEKTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNEKTIQYITKYMTKIDEQHK 179
Query 176 EFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQER 235
+F GK+ SKGIG GYI ++ KH Y+ T ETYR +G K+ LP YY+ +L+T++ER
Sbjct 180 DFIGKVLCSKGIGAGYIKRDDAKKHTYKRGETIETYRLRNGSKINLPIYYRNQLFTEEER 239
Query 236 ESLRIIKEEQQVKY 249
E+L + K E+ + Y
Sbjct 240 EALFLDKIEKGIIY 253
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 213 bits (541), Expect = 7e-68, Method: Compositional matrix adjust.
Identities = 114/254 (45%), Positives = 160/254 (63%), Gaps = 6/254 (2%)
Query 1 VCLYPSIIENPKYA---KSNKNGKRARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+CLYP +I N KY K+N N + D R +I CG C ECR+ K EW VR+ EE+
Sbjct 1 MCLYPKLIRNKKYLPTKKNNYNPPKMVDPRTAYITAACGKCLECRKQKQREWLVRMSEEL 60
Query 58 KSYPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLIT 115
++ P N F TLT S+E+ + L+ ++ N KAI L +R KK +KHW IT
Sbjct 61 RTEP-NAYFMTLTISDENYEILKNICKSEDDNTIATKAIRLMLERIRKKTGKSIKHWFIT 119
Query 116 EMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANP 175
E+GH+ T+R+HLHGI+W T++ E++W YG + G V+EKTINY+ KY+TK DE +P
Sbjct 120 ELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNEKTINYVTKYMTKIDEKHP 179
Query 176 EFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQER 235
+F GK+ S+GIG GY + KH+Y+ T ETYR +G K+ LP YY+ KL+T++ER
Sbjct 180 DFVGKVLCSRGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKER 239
Query 236 ESLRIIKEEQQVKY 249
E L I K E+ + Y
Sbjct 240 ELLFIDKIEKGIIY 253
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 211 bits (538), Expect = 3e-67, Method: Compositional matrix adjust.
Identities = 107/254 (42%), Positives = 157/254 (62%), Gaps = 6/254 (2%)
Query 1 VCLYPSIIENPKYAKSNKNG---KRARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+CLYP +I N +Y + KNG D RL ++ CG C ECR+ K +W VR+ EE+
Sbjct 1 MCLYPKLIRNKRYLPNKKNGGVPPVCPDERLLYVTAACGKCMECRQQKQRQWLVRMSEEL 60
Query 58 KSYPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLIT 115
+ P N F TLT +E+ KL + K+ N+ KA+ L +R KK +KHW IT
Sbjct 61 RQNP-NAYFMTLTIDDENYNKLANICNSKDNNEIATKAVRLMLERIRKKTGKSIKHWFIT 119
Query 116 EMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANP 175
E+GH+ T+R+HLHGI+W T++ ++W YG+++ G V+E TINYI KY+TK D +P
Sbjct 120 ELGHEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNEATINYITKYMTKVDIDHP 179
Query 176 EFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQER 235
+F G++ SKGIG GY + N H+Y T ETYR +G K+ LP YY+ +L++++ER
Sbjct 180 DFVGQVLCSKGIGAGYTKREDANNHKYTKGKTNETYRLRNGAKINLPIYYRNQLFSEEER 239
Query 236 ESLRIIKEEQQVKY 249
E L + K E+ + Y
Sbjct 240 EMLFLDKIEKGIIY 253
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 210 bits (535), Expect = 7e-67, Method: Compositional matrix adjust.
Identities = 117/261 (45%), Positives = 160/261 (61%), Gaps = 8/261 (3%)
Query 1 VCLYPSIIENPKYA---KSNKNGKRARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+CLYP +I N KY K+N N + D R +I CG C ECR+ K EW VR+ EE+
Sbjct 1 MCLYPKLIRNKKYLPTKKNNYNPPKMADPRTAYITAACGKCLECRKQKQREWLVRMSEEL 60
Query 58 KSYPKNIIFATLTFSEES---LKKLEYDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLI 114
++ P N F TLT S+E+ LK + E E N KAI L +R KK ++HW I
Sbjct 61 RTEP-NAYFMTLTISDENYEILKNICKSEDE-NTIATKAIRLMLERIRKKIGKSIRHWFI 118
Query 115 TEMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEAN 174
TE+GH+ T+R+HLHGI+W T++ ++W YG + G V+EKTINYI KY+TK DE +
Sbjct 119 TELGHEKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNEKTINYITKYMTKIDEEH 178
Query 175 PEFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQE 234
P F GK+ SKGIG GY + KH+Y+ T ETYR +G K+ LP YY+ KL+T++E
Sbjct 179 PNFVGKVLCSKGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKE 238
Query 235 RESLRIIKEEQQVKYYNKTPI 255
RE L I K ++ + Y T +
Sbjct 239 RELLFIDKIDKGIIYVLGTKV 259
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 206 bits (523), Expect = 2e-65, Method: Compositional matrix adjust.
Identities = 110/243 (45%), Positives = 152/243 (63%), Gaps = 6/243 (2%)
Query 1 VCLYPSIIENPKYAKSNKNG---KRARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+CLYP +I+N +Y + KN D RLR++ CG C ECR+ K W+VRL EE+
Sbjct 1 MCLYPKLIKNKRYMPTKKNKGVVPPCPDERLRYVTAACGKCYECRKQKGRAWQVRLSEEV 60
Query 58 KSYPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLIT 115
+S P N IF TLT S+ES +K++ Y + +K + LF +R KK K LKHWL T
Sbjct 61 RSDP-NAIFVTLTISDESWEKIKNTYIQLSDEDCIKKMVRLFLERVRKKTKKSLKHWLTT 119
Query 116 EMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANP 175
E G NT+R HLHG+IW E TE + W YG++F G V+E T+NYI KY+TK D+ +
Sbjct 120 ERGGTNTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNECTVNYITKYITKTDKKHK 179
Query 176 EFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQER 235
+F S GIG GY++++ +R+++ T ETYR +G KL LP YY+ KL+TD+ER
Sbjct 180 DFEPITLCSAGIGKGYLSRSDSELNRFREGKTTETYRLRNGTKLNLPIYYRNKLYTDEER 239
Query 236 ESL 238
E L
Sbjct 240 EKL 242
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 192 bits (488), Expect = 3e-60, Method: Compositional matrix adjust.
Identities = 105/252 (42%), Positives = 155/252 (62%), Gaps = 7/252 (3%)
Query 1 VCLYPSIIENPKYA---KSNKNGKRARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+CLY IENPKY K+N N DRRL ++ + CG C ECR+ K W VRL EE+
Sbjct 1 MCLYTKYIENPKYKPNKKNNYNPPVCEDRRLFYVPVKCGKCIECRQQKQRAWIVRLSEEL 60
Query 58 KSYPKNIIFATLTFSEESLKKLEYDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEM 117
+S +F TLTF+EES K+L K N + A+ + + +KYK ++HW +TE+
Sbjct 61 RSGKGAGLFVTLTFNEESYKELAAITKNENDMCRLALYRMNENYRQKYKHTIRHWCVTEI 120
Query 118 GHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEF 177
G D RIH+HGI+W ++ E+ W YG+I+ G V+E+TI YI KY+ K + F
Sbjct 121 GDDG--RIHIHGIMWCPASD--IERYWKYGYIYIGRFVNEQTILYITKYMLKYTPVDKNF 176
Query 178 NGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERES 237
K+ SKGIGI Y+++ ++ Y++ T+E+Y SG K+ LP YYK+K++T++ERE
Sbjct 177 EPKVLCSKGIGINYLDRLDSKRNTYRENNTDESYMLRSGRKINLPDYYKRKIYTEEEREK 236
Query 238 LRIIKEEQQVKY 249
L I K+E+ +Y
Sbjct 237 LWIEKQEKGYRY 248
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 186 bits (473), Expect = 6e-58, Method: Compositional matrix adjust.
Identities = 113/285 (40%), Positives = 167/285 (59%), Gaps = 21/285 (7%)
Query 3 LYPSIIENPKYAKSNKNG---KRARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEIKS 59
+Y I N KY + KN RD RLR++ CG C ECR+ KA WR+RL EE+K+
Sbjct 1 MYTKYILNKKYMYTKKNQGNIPECRDERLRYVPTKCGKCIECRKEKARNWRIRLAEELKN 60
Query 60 YPKNIIFATLTFSEESLKKLEY----------DEKEPNKAPQKAISLFRKRWWKKYKTPL 109
P N +F TLTF+EE+ ++L + + E N+ + A+ + +R KK K +
Sbjct 61 NP-NALFITLTFNEENYQRLSWELFKKSKDNLNYTEQNEMCKTAVRRWLERIRKKTKKSI 119
Query 110 KHWLITEMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTK 169
+HW++TE G D RIHLHGI+W +E+ E +WGYG+ + G V+E TI Y+ KY+ K
Sbjct 120 RHWMVTEKGED-YGRIHLHGIVWC--PKERIE-QWGYGYTYIGDYVNETTIAYVTKYMLK 175
Query 170 RDEANPEFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKL 229
E P+F GK+ S GIG Y + ++RY+ K T+ETY+ +G +L LP YY K+
Sbjct 176 ICEKWPDFRGKVMCSAGIGCRYETSYNAKRNRYRGKETKETYKLENGRELPLPKYYHDKI 235
Query 230 WTDQERESLRIIKEEQQVKYYNKTPIKVETLEQYKEYVNAVMYWQ 274
+T++ERE L IIK+E+ +Y + + LE E+ N Y+Q
Sbjct 236 YTEEEREKLWIIKQERGYRYIAGEKVSTDNLE---EWDNLTKYYQ 277
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 184 bits (468), Expect = 1e-56, Method: Compositional matrix adjust.
Identities = 107/280 (38%), Positives = 159/280 (57%), Gaps = 12/280 (4%)
Query 1 VCLYPSIIENPKYAKSNKNGKR---ARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+C +P I+N ++ + KNG D RLR+I++ CG+C ECR+ K N WRVR E++
Sbjct 1 MCYFPIRIKNKRFVPTKKNGYEPPVCTDERLRYIEVECGYCFECRKKKRNAWRVRNFEQL 60
Query 58 KSYPKNIIF-ATLTFSEESLKKLEYDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITE 116
+ P I F T++ K +Y+ K N+ K LF +R K+ +KHW +TE
Sbjct 61 RETPTAIFFTGTVSPERYDYIKEKYNLKTDNEIITKIHRLFLERIRKETGKSMKHWCVTE 120
Query 117 MGHDNTKRIHLHGIIWTELTEEQFE------KEWGYGWIFFGYEVSEKTINYIVKYVTKR 170
GH NT+RIHLHGI + QF+ W G+ + G +EKTINY+ KY+TK+
Sbjct 121 KGHTNTRRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYNGKYCNEKTINYVSKYMTKK 180
Query 171 DEANPEFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLW 230
D NPE+ GK+ S G+G GY+ K +H + ++ T+E Y G +ALP YYK KL+
Sbjct 181 DMDNPEYTGKVLCSPGLGAGYV-KRIGKRHEWNEENTKEDYYTRQGTYIALPKYYKYKLF 239
Query 231 TDQERESLRIIKEEQQVKYYNKTPIKVETLEQYKEYVNAV 270
T+ +RE L I +E K+ +K+ T E+ +EY N +
Sbjct 240 TEDQREQLWIYRENSGEKFVGNFKVKI-TDEESEEYYNVL 278
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 177 bits (449), Expect = 9e-55, Method: Compositional matrix adjust.
Identities = 96/231 (42%), Positives = 143/231 (62%), Gaps = 3/231 (1%)
Query 40 ECRRAKANEWRVRLMEEIKSYPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLF 97
ECR+ K +W VR+ EE++ P N F TLT ++S +L Y+ + N+ KAI L
Sbjct 2 ECRQQKQRQWLVRMSEELRQEP-NAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLC 60
Query 98 RKRWWKKYKTPLKHWLITEMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSE 157
+R K+ +KHW ITE+GH+ T+R+HLHGI+W T++ +W YG F G+ V+E
Sbjct 61 LERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNE 120
Query 158 KTINYIVKYVTKRDEANPEFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGI 217
KTI YI KY+TK DE + +F GK+ SKGIG GYI ++ KH Y+ T ETYR +G
Sbjct 121 KTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGS 180
Query 218 KLALPTYYKQKLWTDQERESLRIIKEEQQVKYYNKTPIKVETLEQYKEYVN 268
K+ LP YY+ +L+T++E+E+L + K E+ + Y + + E Y + +N
Sbjct 181 KINLPIYYRNQLFTEEEKEALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLN 231
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 169 bits (427), Expect = 1e-50, Method: Compositional matrix adjust.
Identities = 111/279 (40%), Positives = 160/279 (57%), Gaps = 17/279 (6%)
Query 1 VCLYPSIIENPKYAKSNKNGKR---ARDRRLRWIQIPCGHCEECRRAKANEWRVRLMEEI 57
+CLYP+ I+NPKY + KN +DRRL +I + CG C ECR+ K EWRVRL EE+
Sbjct 1 MCLYPTFIKNPKYKPNKKNKGNPPVCKDRRLLYIPVKCGCCIECRKEKQREWRVRLEEEM 60
Query 58 KSYPKNIIFATLTFSEESLKKLEYD-----EKEPNKAPQKAISLFRKRWWKKYKTPLKHW 112
S F TLT +E++KK+E E+ PN+ KA+ LF +R K ++HW
Sbjct 61 HSNFG--YFTTLTIDQENIKKIESITGLKWEENPNEIATKALRLFLERTRKDTGKSIRHW 118
Query 113 LITEMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDE 172
+TE+G N RIHLHGI + + + E K W YG+IF G + K+INYI KY+ K D
Sbjct 119 CVTELGEKNN-RIHLHGIFFGQKSAELIRKHWNYGFIFIGGYCNSKSINYITKYMLKVDI 177
Query 173 ANPEFNGKIFTSKGIGIGYINKNSL--NKHRYQDKFTEETYRAASGIKLALPTYYKQKLW 230
+PEF + S GIG GYI++ K Y++ TY +G K+A+P YYK K++
Sbjct 178 KHPEFKQIVLASSGIGKGYIDRLDYLWQKQNYKN-INVATYTFRNGTKMAMPKYYKNKIF 236
Query 231 TDQERESLRIIKEEQQVKYYNKTPIKV---ETLEQYKEY 266
T++ERE + I + + + +K ET++ +EY
Sbjct 237 TEKEREKMWINNLNRGLLWIYGEKVKADDWETIDNLREY 275
Lambda K H a alpha
0.317 0.134 0.414 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 25460135