bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-25_CDS_annotation_glimmer3.pl_2_4
Length=421
Score E
Sequences producing significant alignments: (Bits) Value
Gokush_Human_feces_D_045_Microviridae_AG0399_putative.VP4 185 8e-56
Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4 148 5e-42
Gokush_Human_feces_A_029_Microviridae_AG0222_putative.VP4 137 3e-38
Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4 131 2e-36
Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4 131 2e-36
Gokush_Human_gut_27_035_Microviridae_AG0163_putative.VP4 131 2e-36
Gokush_Human_feces_B_068_Microviridae_AG0328_putative.VP4 129 3e-35
Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4 115 5e-31
Gokush_Human_feces_A_013_Microviridae_AG011_putative.VP4 116 6e-31
Gokush_Bourget_248_Microviridae_AG0249_putative.VP4 114 1e-30
> Gokush_Human_feces_D_045_Microviridae_AG0399_putative.VP4
Length=329
Score = 185 bits (469), Expect = 8e-56, Method: Compositional matrix adjust.
Identities = 139/416 (33%), Positives = 198/416 (48%), Gaps = 100/416 (24%)
Query 4 MACFHPNHVVVR------DGQFQFLGSAAYLKSGDLFYD-----NPVSFHTLVPCGRCMG 52
M+CFHP + R + Q+ FLG A + L D + S+H +VPC +C+G
Sbjct 1 MSCFHPRVMGCRVDPYTGEVQYNFLGVAESGFNPSLAGDVSSLSSDGSYHFIVPCRKCLG 60
Query 53 CRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplvld 112
C++D SR W DRM+ EL DN KA+F+TLTYND L +
Sbjct 61 CQIDYSREWRDRMIFELHDNP-KAIFLTLTYNDAHLSFS--------------------- 98
Query 113 ddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAIIYG 172
TL + D Q FMKRLRK F R+RF+LAGEYG + RPHYHAI+YG
Sbjct 99 ---------DKGCPTLVVSDVQKFMKRLRKHFTGTRIRFYLAGEYGTQNLRPHYHAILYG 149
Query 173 LTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVYKS 232
+ L+DF D ++ +N+L Q Y S ERIW NG+ +++ V TCAYV+RY MKK K+
Sbjct 150 IDLADFPDLYLRGYNELKQAYYSSPIMERIWKNGFIIMSEVTSRTCAYVARYVMKKQGKN 209
Query 233 ENSHAYASGQLPPFCTMSRRPGIGLLHADDLLKKGDKTFIRDIDLNGKECTREVYLGRAF 292
+ H + G LP F SR PG+GLL + D + G+ TF D G++ + + L ++
Sbjct 210 SDVHV-SRGTLPEFNLSSRNPGLGLLKSRDYVLSGNNTFAFD----GRDGSISISLPKSM 264
Query 293 IRSAAREHMKPVFAAADLVESVQTCINELEAESCTEHEDVLDALYICLRAFRHRLDDVIE 352
+R+ R + + D++D +
Sbjct 265 LRNVRR-------------------------NASQKELDIIDRIVY-------------- 285
Query 353 LHIPPSVRLCSRQNERAYNSIQRSISNLAVFGGYLLEYYQGKELHFLQRIKLLPER 408
R+ ++ R SNL V G ++ +GKE +IKLLPER
Sbjct 286 --------------NRSMDAAARLRSNLDVLGLEFGDFLKGKEKSLTSKIKLLPER 327
> Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4
Length=340
Score = 148 bits (373), Expect = 5e-42, Method: Compositional matrix adjust.
Identities = 102/276 (37%), Positives = 147/276 (53%), Gaps = 28/276 (10%)
Query 45 VPCGRCMGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYe 104
+PCG+C+GCRLD SR WA+R +LEL+D+ A FVTLTY+D+ LP + +V + F
Sbjct 61 IPCGKCVGCRLDYSREWANRCMLELEDSS-NAWFVTLTYDDQHLPRSAYVEPETGEAF-- 117
Query 105 dvvplvldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRP 164
A +L D QLFMKRLR F D ++RFF AGEYG +HRP
Sbjct 118 -------------------ASYSLRKTDFQLFMKRLRYYFPDNKIRFFAAGEYGSHSHRP 158
Query 165 HYHAIIYGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRY 224
HYHAI+Y + DF D + G + SK + W G+ V+ V W +CAYV+RY
Sbjct 159 HYHAILYNV---DFDDLEFYKKSLNGDIYWNSKKLDAAWNKGFAVIGEVTWQSCAYVARY 215
Query 225 TMKKVYKSENSHAYASGQLPPFCTMSRRPGIGLLHADDLLKKGDKTFIRDIDLNGKECTR 284
MKK + S+ P F MSR+PGIG ++ D K D + I ++ + +
Sbjct 216 CMKKADGVDASYYEHFNIEPEFTLMSRKPGIGRMYLD---KHPDLYQYQKIFVSTPQGGK 272
Query 285 EVYLGRAFIRSAAREHMKPVFAAADLVESVQTCINE 320
E+ + + F R A+E+ + + A + ++ NE
Sbjct 273 EITIPKYFDRIVAQENPEMIEALKEKRKAAAIAKNE 308
> Gokush_Human_feces_A_029_Microviridae_AG0222_putative.VP4
Length=343
Score = 137 bits (346), Expect = 3e-38, Method: Compositional matrix adjust.
Identities = 111/279 (40%), Positives = 141/279 (51%), Gaps = 38/279 (14%)
Query 4 MACFHPNHVVVRDGQFQFLGSAAYLKSGDLFYDNPVSFH----TLVPCGRCMGCRLDRSR 59
M C+HP +R G+ G A G PV +PCGRC+GCRL+ SR
Sbjct 16 MPCYHP-MFGIRTGEKTVNGKDAIKIVGAF---KPVGVPDWKIVQIPCGRCIGCRLEYSR 71
Query 60 VWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplvldddeeWIa 119
WA+R +LEL+ +D A FVT+TY+D +P + S +
Sbjct 72 QWANRCMLELQYHD-SAYFVTVTYDDEHVPQTYSSDSETGEAL----------------- 113
Query 120 aaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAIIYGLTLSDFK 179
TLS RD QLFMKR+RK F D R+R+FLAGEYG T RPHYH I++GL L D
Sbjct 114 ---LPLMTLSKRDMQLFMKRVRKRFCDDRIRYFLAGEYGSTTFRPHYHCILFGLHLYDLV 170
Query 180 DCRIKDFNKLGQPRYISKSFERIWGN------GYCVLAPVNWNTCAYVSRYTMKKVYKSE 233
K+F G Y S+S W + GY V+APV + TCAYV+RYT KK ++
Sbjct 171 P-YAKNFR--GDVLYNSQSLSACWCDKSARPMGYVVVAPVTYETCAYVARYTSKKSGVND 227
Query 234 NSHAYASGQLPPFCTMSRRPGIGLLHADDLLKKGDKTFI 272
G PF MSR+PGIG + DD D FI
Sbjct 228 LEAYDLLGLARPFTLMSRKPGIGRQYFDDHPDCMDYDFI 266
> Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4
Length=310
Score = 131 bits (330), Expect = 2e-36, Method: Compositional matrix adjust.
Identities = 87/226 (38%), Positives = 120/226 (53%), Gaps = 24/226 (11%)
Query 38 PVSFHTLVPCGRCMGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSN 97
P S +PCG+C+GCR+D SR WA+R LLELK +D A F T TY+D +P ++
Sbjct 21 PRSALVPLPCGQCIGCRIDYSRQWANRCLLELKYHD-SAWFCTFTYDDDHVPRTYYPDPE 79
Query 98 YFDGFYedvvplvldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEY 157
+ TL RD QL MKR+RK F + ++RFF++GEY
Sbjct 80 TGEAI---------------------PALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEY 118
Query 158 GPKTHRPHYHAIIYGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNT 217
G +T RPHYHAI++GL L D + + Y S S + W GY V+ V W +
Sbjct 119 GSQTFRPHYHAILFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWES 178
Query 218 CAYVSRYTMKKVYKSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 262
CAY +RY MKK+ K + + Y + P F MSR+PGI + D+
Sbjct 179 CAYTARYVMKKL-KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDE 223
> Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4
Length=310
Score = 131 bits (330), Expect = 2e-36, Method: Compositional matrix adjust.
Identities = 87/226 (38%), Positives = 120/226 (53%), Gaps = 24/226 (11%)
Query 38 PVSFHTLVPCGRCMGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSN 97
P S +PCG+C+GCR+D SR WA+R LLELK +D A F T TY+D +P ++
Sbjct 21 PRSALVPLPCGQCIGCRIDYSRQWANRCLLELKYHD-SAWFCTFTYDDDHVPRTYYPDPE 79
Query 98 YFDGFYedvvplvldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEY 157
+ TL RD QL MKR+RK F + ++RFF++GEY
Sbjct 80 TGEAI---------------------PALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEY 118
Query 158 GPKTHRPHYHAIIYGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNT 217
G +T RPHYHAI++GL L D + + Y S S + W GY V+ V W +
Sbjct 119 GSQTFRPHYHAILFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWES 178
Query 218 CAYVSRYTMKKVYKSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 262
CAY +RY MKK+ K + + Y + P F MSR+PGI + D+
Sbjct 179 CAYTARYVMKKL-KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDE 223
> Gokush_Human_gut_27_035_Microviridae_AG0163_putative.VP4
Length=310
Score = 131 bits (330), Expect = 2e-36, Method: Compositional matrix adjust.
Identities = 87/226 (38%), Positives = 120/226 (53%), Gaps = 24/226 (11%)
Query 38 PVSFHTLVPCGRCMGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSN 97
P S +PCG+C+GCR+D SR WA+R LLELK +D A F T TY+D +P ++
Sbjct 21 PRSALVPLPCGQCIGCRIDYSRQWANRCLLELKYHD-SAWFCTFTYDDDHVPRTYYPDPE 79
Query 98 YFDGFYedvvplvldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEY 157
+ TL RD QL MKR+RK F + ++RFF++GEY
Sbjct 80 TGEAI---------------------PALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEY 118
Query 158 GPKTHRPHYHAIIYGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNT 217
G +T RPHYHAI++GL L D + + Y S S + W GY V+ V W +
Sbjct 119 GSQTFRPHYHAILFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWES 178
Query 218 CAYVSRYTMKKVYKSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 262
CAY +RY MKK+ K + + Y + P F MSR+PGI + D+
Sbjct 179 CAYTARYVMKKL-KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDE 223
> Gokush_Human_feces_B_068_Microviridae_AG0328_putative.VP4
Length=351
Score = 129 bits (323), Expect = 3e-35, Method: Compositional matrix adjust.
Identities = 95/234 (41%), Positives = 127/234 (54%), Gaps = 35/234 (15%)
Query 39 VSFHTLVPCGRCMGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNY 98
V+ ++PCG+C+GCRL+ SR WA+R +LEL + + FVTLTY+D LP +++ +
Sbjct 56 VAQSVIIPCGKCLGCRLEYSRQWANRCMLELGYH-VSSWFVTLTYDDAHLPRSFYGNPDT 114
Query 99 FDGFYedvvplvldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYG 158
+ ATL RD QLFMKRLR F D + AGEYG
Sbjct 115 GEAV---------------------PCATLYKRDFQLFMKRLRYKFGDGIRFYA-AGEYG 152
Query 159 PKTHRPHYHAIIYGLTLSD---FKDCRIKDFNKLGQPRYISKSFERIW----GN--GYCV 209
+T RPHYHAIIYGL L D +K ++ N L Y S+S + W GN G+ V
Sbjct 153 DQTKRPHYHAIIYGLELDDLVFYKKMALESAN-LYYNYYNSESLQSCWRDKDGNDIGFVV 211
Query 210 LAPVNWNTCAYVSRYTMKKVYKSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 262
+ V W TCAYV+RY MKK K + + Y + P FC MSR+PGI + +D
Sbjct 212 VGKVTWETCAYVARYIMKKQ-KGQGADVYERFNIEPEFCLMSRKPGIAHQYYED 264
> Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4
Length=305
Score = 115 bits (289), Expect = 5e-31, Method: Compositional matrix adjust.
Identities = 90/259 (35%), Positives = 127/259 (49%), Gaps = 51/259 (20%)
Query 4 MACFHPNHVVVRDGQFQFLGSAAYLKSGDLFYDN--PVSFHTL-VPCGRCMGCRLDRSRV 60
M CFHP GQ G +S + +D+ + F +PCG+C+GCRL +SR
Sbjct 1 MPCFHPITAYRLAGQKTKDG-----RSNAVTFDSSKAIPFSEFKIPCGQCIGCRLSKSRE 55
Query 61 WADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplvldddeeWIaa 120
WA R ++E K + +F+TLTY+D LP GS +++ F
Sbjct 56 WAARCVVEAKSHK-NNMFLTLTYDDAHLPED---GSLHYEHF------------------ 93
Query 121 aagapaTLSIRDTQLFMKrlrktfrdrrlrff---lAGEYGPKTHRPHYHAIIYGLTLSD 177
QLFMKR+RK F R + + GEYG K RPHYHAII+G+T D
Sbjct 94 -------------QLFMKRMRKYFMSRFGQQLRFFMCGEYGDKLGRPHYHAIIFGVTFVD 140
Query 178 FKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVYKSENSHA 237
+ I+ N L Y S++ E++W G+ + VN+ T AYV+RY KK+
Sbjct 141 KQLWSIRRGNNL----YRSRTLEKLWPYGFSSIGAVNFETAAYVARYVTKKITGPLKLDH 196
Query 238 YASGQLPPFCTMSRRPGIG 256
Y G++ FC S +PGIG
Sbjct 197 Y-DGKVAEFCHCSLKPGIG 214
> Gokush_Human_feces_A_013_Microviridae_AG011_putative.VP4
Length=330
Score = 116 bits (290), Expect = 6e-31, Method: Compositional matrix adjust.
Identities = 88/260 (34%), Positives = 124/260 (48%), Gaps = 31/260 (12%)
Query 4 MACFHPNHVVVRDGQFQFLGSAAYLKSGDLFYDNPVSFHTL-VPCGRCMGCRLDRSRVWA 62
M+C+HP V +F + + D F + +PCG+C+GCR++ SR WA
Sbjct 1 MSCYHP--VYAYKSKFVNPETGKAVIKFHPRPDQMDKFEPIALPCGQCLGCRIEYSRQWA 58
Query 63 DRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplvldddeeWIaaaa 122
+R++LE + +D A F T TY+D +P +++ +
Sbjct 59 NRLMLEREAHD-AAWFCTFTYDDDHVPRSYYPDPETGEAI-------------------- 97
Query 123 gapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAIIYGLTLSDFKDCR 182
TL RD QL MKR+R+ F D +RFF GEYG +T RPHYHAII+GL L D +
Sbjct 98 -PSLTLCKRDFQLLMKRIRRRFPDDHIRFFACGEYGSQTFRPHYHAIIFGLHLDDLVPYK 156
Query 183 IKDFNKLGQPRYISKSFERIW----GN--GYCVLAPVNWNTCAYVSRYTMKKVYKSENSH 236
Y S + W GN G+ V+ V W +CAY +RY KK+ + E+
Sbjct 157 TVREGGELYTYYNSPKLQSCWLDSDGNPIGFVVVGEVTWESCAYTARYVTKKLNRKEHDF 216
Query 237 AYASGQLPPFCTMSRRPGIG 256
P F MSRRPGI
Sbjct 217 YEKHRICPEFSLMSRRPGIA 236
> Gokush_Bourget_248_Microviridae_AG0249_putative.VP4
Length=299
Score = 114 bits (286), Expect = 1e-30, Method: Compositional matrix adjust.
Identities = 94/263 (36%), Positives = 128/263 (49%), Gaps = 58/263 (22%)
Query 4 MACFHP-NHVVVRDGQFQFLGSAAYLKSGDLFYDNPVSFHTLVPCGRCMGCRLDRSRVWA 62
M C+HP + DGQ F + LK D +S +PCG+C+GCRL+RSR WA
Sbjct 1 MPCYHPISAYQCTDGQIVF----SELKRHD------ISRSLNLPCGQCVGCRLERSRQWA 50
Query 63 DRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplvldddeeWIaaaa 122
R + E + + + F+TLTYND +PS
Sbjct 51 IRCMHEAQMHT-QNCFITLTYNDDHIPS-------------------------------- 77
Query 123 gapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAIIYGLTLSDFKDCR 182
+L RD QLF+KRLRK + RR+R+++AGEYG RPH+HA I+GL DF D +
Sbjct 78 --DRSLHYRDFQLFIKRLRKRYPGRRIRYYMAGEYGENLGRPHWHACIFGL---DFDDKK 132
Query 183 IKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVYKSENSHAYA--- 239
+ Y SK+ E +W GY + V + + AYV+RY MKKV Y
Sbjct 133 LWKRTAANSILYRSKNLELLWPFGYSSIGDVTFESAAYVARYIMKKVTGKNAEQHYTEID 192
Query 240 --SGQL----PPFCTMSRRPGIG 256
SG++ P F MS +PGIG
Sbjct 193 PESGEITIRKPEFTKMSLKPGIG 215
Lambda K H a alpha
0.325 0.140 0.446 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 37315600