bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-4_CDS_annotation_glimmer3.pl_2_1
Length=128
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4 215 3e-69
Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4 36.6 2e-05
Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4 26.6 0.061
Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4 26.2 0.093
Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4 23.5 0.68
Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4 21.2 3.9
Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4 21.2 4.2
Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4 21.2 4.2
Microvirus_gi|9626373|ref|NP_040703.1|_DNA_replication_initiati... 21.2 4.8
Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4 20.8 5.0
> Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4
Length=563
Score = 215 bits (548), Expect = 3e-69, Method: Compositional matrix adjust.
Identities = 103/128 (80%), Positives = 113/128 (88%), Gaps = 0/128 (0%)
Query 1 MQEFLHIVRLDGYSFLNWDVPIGKISRFFYRFNRFEAMKGSLRSKLKAVSLFYDYRDYQS 60
MQEFLHIVRLD SFLNWD+P GKISRF YRFN FE MKGS RSKLKAV LFYDYRDYQS
Sbjct 436 MQEFLHIVRLDRESFLNWDIPKGKISRFLYRFNMFEKMKGSFRSKLKAVELFYDYRDYQS 495
Query 61 LKNQLSLQELVFAELGYSDELFDSFYVKPHIKVLKNAYIDKWKDVNYKEVHYFRVKHKVL 120
LKNQL +Q+LVF+ELGYSDEL DSFYVKP++KVLKN Y +KW D NY EVHYFRVKHKVL
Sbjct 496 LKNQLYMQQLVFSELGYSDELLDSFYVKPNLKVLKNIYTEKWHDTNYHEVHYFRVKHKVL 555
Query 121 NDENNIFL 128
ND+N++FL
Sbjct 556 NDQNDVFL 563
> Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4
Length=539
Score = 36.6 bits (83), Expect = 2e-05, Method: Compositional matrix adjust.
Identities = 34/135 (25%), Positives = 55/135 (41%), Gaps = 47/135 (35%)
Query 17 NW-DVPIGKISRFFYRFNRFEAMKGSLRSKLKAVSLFYDYRDYQSLKNQL---------- 65
NW P+G + A G L ++A+ FYDY +SL +QL
Sbjct 421 NWITAPVGSV-----------AFTGQLHRAVRAIHSFYDYCSRRSLHDQLVKVEKWSNDS 469
Query 66 ------------SLQELVFAELGYSDELFDSFYVKPHIKVLKNAYIDKWKDVNYKEVHYF 113
L ++ +S+ +FDS +VL+ +Y D Y +
Sbjct 470 YVRANLSIYYFYPLTDVDIMMKSFSEIVFDS-------QVLRASYAD------YAADNRE 516
Query 114 RVKHKVLNDENNIFL 128
R+KHK LND+N++ +
Sbjct 517 RIKHKALNDKNSLLI 531
> Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4
Length=536
Score = 26.6 bits (57), Expect = 0.061, Method: Compositional matrix adjust.
Identities = 27/102 (26%), Positives = 47/102 (46%), Gaps = 11/102 (11%)
Query 37 AMKGSLRSKLKAVSLFYDYRDYQSLKNQLSLQELVFAELGYSDELFDSFYVKPHI--KVL 94
A G LR + + FY Y D + L +QL ++ +++ Y D Y P +++
Sbjct 430 AFYGQLRKAFRTIYAFYSYMDSKYLHDQL-VKVQSWSQNNYLSTQVDFRYFYPLTDGELM 488
Query 95 KNAY-----IDKWKDVNYKEV---HYFRVKHKVLNDENNIFL 128
+++Y + + Y EV + R+KHK LND N +
Sbjct 489 RSSYREVLALSSFCRAAYAEVAADNRQRIKHKYLNDLNCALI 530
> Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4
Length=559
Score = 26.2 bits (56), Expect = 0.093, Method: Compositional matrix adjust.
Identities = 30/118 (25%), Positives = 50/118 (42%), Gaps = 16/118 (14%)
Query 15 FLNWDVPIGKISRFFYRFNRFEAMKGSLRSKLKAVSLFYDYRDYQSLKNQLSLQELVFAE 74
F NW +P FF + A G + +K + +DY+SL++ +++ A
Sbjct 449 FRNWHLP------FF--GSDISAFSGRIMFIIKTGIEYEKKKDYESLRDVFNIRS---AN 497
Query 75 LGYSDELFDSFYVKPHIKVLKNAYIDK---WKDVNYKEVHYFR--VKHKVLNDENNIF 127
SD +F VL + + + + + + R +KHK LND NNIF
Sbjct 498 PNISDCMFALPANGQERDVLSDVSCETVQLLEQLRLRSATFCRDMIKHKRLNDANNIF 555
> Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4
Length=562
Score = 23.5 bits (49), Expect = 0.68, Method: Compositional matrix adjust.
Identities = 14/40 (35%), Positives = 21/40 (53%), Gaps = 9/40 (23%)
Query 89 PHIKVLKNAYIDKWKDVNYKEVHYFRVKHKVLNDENNIFL 128
P ++ L A + K +D +VKHK +ND + IFL
Sbjct 532 PLVRRLAAASLMKCRD---------KVKHKKVNDLSGIFL 562
> Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4
Length=532
Score = 21.2 bits (43), Expect = 3.9, Method: Compositional matrix adjust.
Identities = 17/65 (26%), Positives = 32/65 (49%), Gaps = 4/65 (6%)
Query 66 SLQELVFAELGYSDELFDSFYVKPHIKV---LKNAYIDKWKDVNYKEVHYFRVKHKVLND 122
SLQ+L + + D F+ + K+ + + Y + +D + E+ R+KH+ +ND
Sbjct 458 SLQDLFHDSEAFESDWSDIFWDRRQEKIKRFVDSDYGNLCRDKLHNEIRK-RIKHREIND 516
Query 123 ENNIF 127
IF
Sbjct 517 AVGIF 521
> Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4
Length=611
Score = 21.2 bits (43), Expect = 4.2, Method: Compositional matrix adjust.
Identities = 10/28 (36%), Positives = 14/28 (50%), Gaps = 0/28 (0%)
Query 101 KWKDVNYKEVHYFRVKHKVLNDENNIFL 128
KW ++V +VKHK ND + L
Sbjct 581 KWLTARTEKVLKDKVKHKEFNDMQGVLL 608
> Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4
Length=547
Score = 21.2 bits (43), Expect = 4.2, Method: Compositional matrix adjust.
Identities = 9/14 (64%), Positives = 10/14 (71%), Gaps = 0/14 (0%)
Query 115 VKHKVLNDENNIFL 128
+KHK LND N FL
Sbjct 532 IKHKELNDLNYCFL 545
> Microvirus_gi|9626373|ref|NP_040703.1|_DNA_replication_initiation_protein_gpA_[Enterobacteria_phage_phiX174_sensu_lato]
Length=513
Score = 21.2 bits (43), Expect = 4.8, Method: Composition-based stats.
Identities = 11/36 (31%), Positives = 19/36 (53%), Gaps = 6/36 (17%)
Query 70 LVFAELGYSDELFDSFYVKPHIKVLKNAYIDKWKDV 105
+VF L +D+ ++FY P NA D ++D+
Sbjct 198 IVFDTLTLADDRLEAFYDNP------NALRDYFRDI 227
> Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4
Length=532
Score = 20.8 bits (42), Expect = 5.0, Method: Compositional matrix adjust.
Identities = 17/65 (26%), Positives = 31/65 (48%), Gaps = 4/65 (6%)
Query 66 SLQELVFAELGYSDELFDSFYVKPHIKV---LKNAYIDKWKDVNYKEVHYFRVKHKVLND 122
SLQ+L + + D F+ + K+ + + Y +D + E+ R+KH+ +ND
Sbjct 458 SLQDLFHDSEAFESDWSDIFWDRRQEKIKRFVDSDYGSLCRDKLHSEIRK-RIKHREIND 516
Query 123 ENNIF 127
IF
Sbjct 517 AVGIF 521
Lambda K H a alpha
0.326 0.142 0.430 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 7679988