bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-8_CDS_annotation_glimmer3.pl_2_7
Length=322
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 542 0.0
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 201 3e-63
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 195 1e-60
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 194 3e-60
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 189 3e-59
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 191 4e-59
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 185 3e-57
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 172 7e-52
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 169 3e-51
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 165 2e-49
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 542 bits (1396), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 269/322 (84%), Positives = 296/322 (92%), Gaps = 1/322 (0%)
Query 1 LEEELHSSFGYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKSI 60
LEEE+HS+FGYFTTLTI E IK+IE TGLKWEENPNEIATK LRLFLER RK+TGKSI
Sbjct 56 LEEEMHSNFGYFTTLTIDQENIKKIESITGLKWEENPNEIATKALRLFLERTRKDTGKSI 115
Query 61 KHWCVTELGEKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYMLKV 120
+HWCVTELGEK +RIHLHGIFFGQKSAELIRKHW YGF+FIGGYCNS+S+NYITKYMLKV
Sbjct 116 RHWCVTELGEKNNRIHLHGIFFGQKSAELIRKHWNYGFIFIGGYCNSKSINYITKYMLKV 175
Query 121 DIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKNINVATYTFRNGTKMAMPKYYKNKI 180
DIKHPEFKQIVLAS+GIG GY+DRLDYLWQKQNYKNINVATYTFRNGTKMAMPKYYKNKI
Sbjct 176 DIKHPEFKQIVLASSGIGKGYIDRLDYLWQKQNYKNINVATYTFRNGTKMAMPKYYKNKI 235
Query 181 FTEKEREKMWINNLNRGLIWIYGEKVKADDWKTIDNLRAYWQKYGCEVMGDNPIAWNAMk 240
FTEKEREKMWINNLNRGL+WIYGEKVKADDW+TIDNLR YWQKYG E MGDNPIAWNAMK
Sbjct 236 FTEKEREKMWINNLNRGLLWIYGEKVKADDWETIDNLREYWQKYGRETMGDNPIAWNAMK 295
Query 241 errkeekqrrAVALAKKQAEKFSTENLIEELPLQADFPMQEEKQEWDKLIEKYIQSNYWL 300
ERRKEEKQRRA+A AKK AEKFSTENL ELPLQADFPMQE++++W+ +I +Y++ N W
Sbjct 296 ERRKEEKQRRAIAEAKKLAEKFSTENLT-ELPLQADFPMQEKEEKWEDIINQYVKDNMWT 354
Query 301 FERVRRCATSELFDALGVMEQA 322
FERVR+C T+E +DALG +E A
Sbjct 355 FERVRKCTTAEHYDALGEIEVA 376
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 201 bits (512), Expect = 3e-63, Method: Compositional matrix adjust.
Identities = 110/238 (46%), Positives = 151/238 (63%), Gaps = 6/238 (3%)
Query 1 LEEELHS-SFGYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKS 59
+ EEL + YF TLTIS E + ++ + E+ N IATK +RL LER+RK+TGKS
Sbjct 56 MSEELRTEPNAYFMTLTISDENYEILK---NICKSEDDNTIATKAIRLMLERIRKKTGKS 112
Query 60 IKHWCVTELG-EKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYML 118
IKHW +TELG EK +R+HLHGI +G + +LI + W YG + G Y N +++NY+TKYM
Sbjct 113 IKHWFITELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNEKTINYVTKYMT 172
Query 119 KVDIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKNINVATYTFRNGTKMAMPKYYKN 178
K+D KHP+F VL S GIGAGY R D K K + TY RNG K+ +P YY+N
Sbjct 173 KIDEKHPDFVGKVLCSRGIGAGYTKRPDAAKHKYK-KGETIETYRLRNGAKINLPIYYRN 231
Query 179 KIFTEKEREKMWINNLNRGLIWIYGEKVKADDWKTIDNLRAYWQKYGCEVMGDNPIAW 236
K+FTEKERE ++I+ + +G+I++ G+KV DD + L +K C + G N W
Sbjct 232 KLFTEKERELLFIDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRLYGYNLQNW 289
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 195 bits (495), Expect = 1e-60, Method: Compositional matrix adjust.
Identities = 103/214 (48%), Positives = 142/214 (66%), Gaps = 6/214 (3%)
Query 1 LEEELHS-SFGYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKS 59
+ EEL + YF TLTIS E + ++ + E+ N IATK +RL LER+RK+ GKS
Sbjct 56 MSEELRTEPNAYFMTLTISDENYEILK---NICKSEDENTIATKAIRLMLERIRKKIGKS 112
Query 60 IKHWCVTELG-EKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYML 118
I+HW +TELG EK +R+HLHGI +G + +LIR+ W YG + G + N +++NYITKYM
Sbjct 113 IRHWFITELGHEKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNEKTINYITKYMT 172
Query 119 KVDIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKNINVATYTFRNGTKMAMPKYYKN 178
K+D +HP F VL S GIGAGY R D K K + TY RNG K+ +P YY+N
Sbjct 173 KIDEEHPNFVGKVLCSKGIGAGYTKRPDAAKHKYK-KGETIETYRLRNGAKINLPIYYRN 231
Query 179 KIFTEKEREKMWINNLNRGLIWIYGEKVKADDWK 212
K+FTEKERE ++I+ +++G+I++ G KV DD K
Sbjct 232 KLFTEKERELLFIDKIDKGIIYVLGTKVHRDDEK 265
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 194 bits (492), Expect = 3e-60, Method: Compositional matrix adjust.
Identities = 101/210 (48%), Positives = 138/210 (66%), Gaps = 5/210 (2%)
Query 2 EEELHSSFGYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKSIK 61
EE + YF TLTI E ++ K + NEIATK +RL LER+RK+TGKSIK
Sbjct 58 EELRQNPNAYFMTLTIDDENYNKLANICNSK---DNNEIATKAVRLMLERIRKKTGKSIK 114
Query 62 HWCVTELG-EKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYMLKV 120
HW +TELG EK +R+HLHGI +G + +LI + W YGFV+ G + N ++NYITKYM KV
Sbjct 115 HWFITELGHEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNEATINYITKYMTKV 174
Query 121 DIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKNINVATYTFRNGTKMAMPKYYKNKI 180
DI HP+F VL S GIGAGY R D K N TY RNG K+ +P YY+N++
Sbjct 175 DIDHPDFVGQVLCSKGIGAGYTKREDANNHKYTKGKTN-ETYRLRNGAKINLPIYYRNQL 233
Query 181 FTEKEREKMWINNLNRGLIWIYGEKVKADD 210
F+E+ERE ++++ + +G+I++ G+KV D+
Sbjct 234 FSEEEREMLFLDKIEKGIIYVMGQKVHRDN 263
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 189 bits (481), Expect = 3e-59, Method: Compositional matrix adjust.
Identities = 103/237 (43%), Positives = 147/237 (62%), Gaps = 7/237 (3%)
Query 2 EEELHSSFGYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKSIK 61
EE YF TLTI + E+ + + NEIATK +RL LER+RK+TGKSIK
Sbjct 17 EELRQEPNAYFITLTIDDKSYSELSNTYNIT---DNNEIATKAIRLCLERIRKQTGKSIK 73
Query 62 HWCVTELG-EKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYMLKV 120
HW +TELG EK +R+HLHGI +G + +LI W YG F G + N +++ YITKYM K+
Sbjct 74 HWFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNEKTIQYITKYMTKI 133
Query 121 DIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYK-NINVATYTFRNGTKMAMPKYYKNK 179
D +H +F VL S GIGAGY+ R D +K YK + TY RNG+K+ +P YY+N+
Sbjct 134 DEQHKDFIGKVLCSKGIGAGYIKRDDA--KKHTYKPGETIETYRLRNGSKINLPIYYRNQ 191
Query 180 IFTEKEREKMWINNLNRGLIWIYGEKVKADDWKTIDNLRAYWQKYGCEVMGDNPIAW 236
+FTE+E+E ++++ + +G+I++ G+KV DD + L +K C + G N W
Sbjct 192 LFTEEEKEALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLNEGRKTECRLYGYNLQNW 248
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 191 bits (484), Expect = 4e-59, Method: Compositional matrix adjust.
Identities = 104/237 (44%), Positives = 147/237 (62%), Gaps = 7/237 (3%)
Query 2 EEELHSSFGYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKSIK 61
EE YF TLTI + E+ + + NEIATK +RL LER+RK+TGKSIK
Sbjct 58 EELRQEPNAYFITLTIDDKSYSELSNTYNIT---DNNEIATKAIRLCLERIRKQTGKSIK 114
Query 62 HWCVTELG-EKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYMLKV 120
HW +TELG EK +R+HLHGI +G + +LI W YG F G + N +++ YITKYM K+
Sbjct 115 HWFITELGHEKTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNEKTIQYITKYMTKI 174
Query 121 DIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKN-INVATYTFRNGTKMAMPKYYKNK 179
D +H +F VL S GIGAGY+ R D +K YK + TY RNG+K+ +P YY+N+
Sbjct 175 DEQHKDFIGKVLCSKGIGAGYIKRDD--AKKHTYKRGETIETYRLRNGSKINLPIYYRNQ 232
Query 180 IFTEKEREKMWINNLNRGLIWIYGEKVKADDWKTIDNLRAYWQKYGCEVMGDNPIAW 236
+FTE+ERE ++++ + +G+I++ G+KV DD + L +K C + G N W
Sbjct 233 LFTEEEREALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRLYGYNLQNW 289
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 185 bits (469), Expect = 3e-57, Method: Compositional matrix adjust.
Identities = 102/239 (43%), Positives = 147/239 (62%), Gaps = 6/239 (3%)
Query 1 LEEELHSSF-GYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKS 59
L EE+ S F TLTIS E ++I+ T ++ + + K +RLFLERVRK+T KS
Sbjct 56 LSEEVRSDPNAIFVTLTISDESWEKIKN-TYIQLSDE--DCIKKMVRLFLERVRKKTKKS 112
Query 60 IKHWCVTELG-EKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYML 118
+KHW TE G +R HLHG+ +G+ + L + W+YGFVFIG + N +VNYITKY+
Sbjct 113 LKHWLTTERGGTNTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNECTVNYITKYIT 172
Query 119 KVDIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKNINVATYTFRNGTKMAMPKYYKN 178
K D KH +F+ I L SAGIG GY+ R D + + TY RNGTK+ +P YY+N
Sbjct 173 KTDKKHKDFEPITLCSAGIGKGYLSRSDSELNRFR-EGKTTETYRLRNGTKLNLPIYYRN 231
Query 179 KIFTEKEREKMWINNLNRGLIWIYGEKVKADDWKTIDNLRAYWQKYGCEVMGDNPIAWN 237
K++T++EREK+++ + +G +WI G + DWKT + Q ++ GD+PI W+
Sbjct 232 KLYTDEEREKLFLEKIKKGKVWICGRECNIKDWKTYSQILKEEQIKAKQLHGDSPIDWD 290
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 172 bits (436), Expect = 7e-52, Method: Compositional matrix adjust.
Identities = 98/208 (47%), Positives = 131/208 (63%), Gaps = 16/208 (8%)
Query 12 FTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGKSIKHWCVTELGEK 71
F T T+SPE I+EK LK + NEI TK RLFLER+RKETGKS+KHWCVTE G
Sbjct 68 FFTGTVSPERYDYIKEKYNLKTD---NEIITKIHRLFLERIRKETGKSMKHWCVTEKGHT 124
Query 72 KDR-IHLHGIFFGQ------KSAELIRKHWKYGFVFIGGYCNSRSVNYITKYMLKVDIKH 124
R IHLHGIF+ K ++R +W G+ + G YCN +++NY++KYM K D+ +
Sbjct 125 NTRRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYNGKYCNEKTINYVSKYMTKKDMDN 184
Query 125 PEFKQIVLASAGIGAGYMDRLD--YLWQKQNYKNINVATYTFRNGTKMAMPKYYKNKIFT 182
PE+ VL S G+GAGY+ R+ + W ++N K Y R GT +A+PKYYK K+FT
Sbjct 185 PEYTGKVLCSPGLGAGYVKRIGKRHEWNEENTK----EDYYTRQGTYIALPKYYKYKLFT 240
Query 183 EKEREKMWINNLNRGLIWIYGEKVKADD 210
E +RE++WI N G ++ KVK D
Sbjct 241 EDQREQLWIYRENSGEKFVGNFKVKITD 268
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 169 bits (429), Expect = 3e-51, Method: Compositional matrix adjust.
Identities = 103/244 (42%), Positives = 143/244 (59%), Gaps = 14/244 (6%)
Query 1 LEEEL-HSSFGYFTTLTISPEGIKEI------EEKTGLKWEENPNEIATKGLRLFLERVR 53
L EEL ++ F TLT + E + + + K L + E NE+ +R +LER+R
Sbjct 54 LAEELKNNPNALFITLTFNEENYQRLSWELFKKSKDNLNYTEQ-NEMCKTAVRRWLERIR 112
Query 54 KETGKSIKHWCVTELGEKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYI 113
K+T KSI+HW VTE GE RIHLHGI + K E I + W YG+ +IG Y N ++ Y+
Sbjct 113 KKTKKSIRHWMVTEKGEDYGRIHLHGIVWCPK--ERI-EQWGYGYTYIGDYVNETTIAYV 169
Query 114 TKYMLKVDIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKNINVA-TYTFRNGTKMAM 172
TKYMLK+ K P+F+ V+ SAGIG Y Y ++ Y+ TY NG ++ +
Sbjct 170 TKYMLKICEKWPDFRGKVMCSAGIGCRY--ETSYNAKRNRYRGKETKETYKLENGRELPL 227
Query 173 PKYYKNKIFTEKEREKMWINNLNRGLIWIYGEKVKADDWKTIDNLRAYWQKYGCEVMGDN 232
PKYY +KI+TE+EREK+WI RG +I GEKV D+ + DNL Y+QK ++ GD
Sbjct 228 PKYYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTDNLEEWDNLTKYYQKRAEQLYGDK 287
Query 233 PIAW 236
P W
Sbjct 288 PEDW 291
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 165 bits (417), Expect = 2e-49, Method: Compositional matrix adjust.
Identities = 96/240 (40%), Positives = 139/240 (58%), Gaps = 13/240 (5%)
Query 1 LEEELHSS--FGYFTTLTISPEGIKEIEEKTGLKWEENPNEIATKGLRLFLERVRKETGK 58
L EEL S G F TLT + E KE+ T +N N++ L E R++
Sbjct 56 LSEELRSGKGAGLFVTLTFNEESYKELAAIT-----KNENDMCRLALYRMNENYRQKYKH 110
Query 59 SIKHWCVTELGEKKDRIHLHGIFFGQKSAELIRKHWKYGFVFIGGYCNSRSVNYITKYML 118
+I+HWCVTE+G+ RIH+HGI + A I ++WKYG+++IG + N +++ YITKYML
Sbjct 111 TIRHWCVTEIGDD-GRIHIHGIMWC--PASDIERYWKYGYIYIGRFVNEQTILYITKYML 167
Query 119 KVDIKHPEFKQIVLASAGIGAGYMDRLDYLWQKQNYKNINV-ATYTFRNGTKMAMPKYYK 177
K F+ VL S GIG Y+DRLD ++ Y+ N +Y R+G K+ +P YYK
Sbjct 168 KYTPVDKNFEPKVLCSKGIGINYLDRLDS--KRNTYRENNTDESYMLRSGRKINLPDYYK 225
Query 178 NKIFTEKEREKMWINNLNRGLIWIYGEKVKADDWKTIDNLRAYWQKYGCEVMGDNPIAWN 237
KI+TE+EREK+WI +G +I GEKV D+ + + L YW+K E+ + P W+
Sbjct 226 RKIYTEEEREKLWIEKQEKGYRYIMGEKVSTDNEEKVYKLMEYWRKKAKELYNEKPQEWD 285
Lambda K H a alpha
0.319 0.135 0.420 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 27264798