bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-23_CDS_annotation_glimmer3.pl_2_4
Length=291
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 469 1e-168
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 461 4e-165
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 457 1e-163
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 436 5e-156
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 436 2e-155
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 258 5e-86
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 223 5e-72
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 214 1e-68
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 208 7e-66
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 192 1e-59
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 469 bits (1208), Expect = 1e-168, Method: Compositional matrix adjust.
Identities = 236/291 (81%), Positives = 259/291 (89%), Gaps = 0/291 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
MECRQQKQRQWLVRMSEELRQ PNAYFMTLTI DENY L NIC S+D N IATKA+RLM
Sbjct 42 MECRQQKQRQWLVRMSEELRQNPNAYFMTLTIDDENYNKLANICNSKDNNEIATKAVRLM 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIG D+LI EKWNYG +TG FVNE
Sbjct 102 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
TINYITKYMTK D+DHP F+G+VLCSKGIG GY KREDA NH Y KGKT E YRLRNG+
Sbjct 162 ATINYITKYMTKVDIDHPDFVGQVLCSKGIGAGYTKREDANNHKYTKGKTNETYRLRNGA 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEGRRAEQRL 240
KINLPIYYRNQLF+EEEREMLFLDKIEKGIIYVMGQKVHRDNE EY+ LLEE R+ EQRL
Sbjct 222 KINLPIYYRNQLFSEEEREMLFLDKIEKGIIYVMGQKVHRDNEAEYLGLLEEARKTEQRL 281
Query 241 YGNHVQEWEKEKYLNrlrkqkkkqereleqlelYWAFEKAKTYTQIESCPF 291
YG H QEWE++KYLNRLR+Q+K+QER+ ++LE+YWA E+AK YTQ+E+CPF
Sbjct 282 YGVHEQEWEEQKYLNRLRRQRKRQERKTKELEMYWALERAKDYTQLETCPF 332
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 461 bits (1185), Expect = 4e-165, Method: Compositional matrix adjust.
Identities = 235/291 (81%), Positives = 262/291 (90%), Gaps = 0/291 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
+ECR+QKQR+WLVRMSEELR PNAYFMTLTISDENYEILKNICKSED NTIATKAIRLM
Sbjct 42 LECRKQKQREWLVRMSEELRTEPNAYFMTLTISDENYEILKNICKSEDENTIATKAIRLM 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRKK GKSI+HWFITELGHEKTERLHLHGIVWGIG D+LI EKWNYGIT+TG FVNE
Sbjct 102 LERIRKKIGKSIRHWFITELGHEKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TINYITKYMTK D +HP F+GKVLCSKGIG GY KR DA H YKKG+TIE YRLRNG+
Sbjct 162 KTINYITKYMTKIDEEHPNFVGKVLCSKGIGAGYTKRPDAAKHKYKKGETIETYRLRNGA 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEGRRAEQRL 240
KINLPIYYRN+LFTE+ERE+LF+DKI+KGIIYV+G KVHRD+E+ YI LLEEGR+ E L
Sbjct 222 KINLPIYYRNKLFTEKERELLFIDKIDKGIIYVLGTKVHRDDEKYYIQLLEEGRKKENML 281
Query 241 YGNHVQEWEKEKYLNrlrkqkkkqereleqlelYWAFEKAKTYTQIESCPF 291
YGNH QEWE++KYLNRL+KQKKKQE+E+ QLE YWA E+AK YTQ+E CPF
Sbjct 282 YGNHTQEWEQQKYLNRLQKQKKKQEQEINQLETYWALERAKDYTQLEECPF 332
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 457 bits (1176), Expect = 1e-163, Method: Compositional matrix adjust.
Identities = 232/291 (80%), Positives = 263/291 (90%), Gaps = 0/291 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
+ECR+QKQR+WLVRMSEELR PNAYFMTLTISDENYEILKNICKSED NTIATKAIRLM
Sbjct 42 LECRKQKQREWLVRMSEELRTEPNAYFMTLTISDENYEILKNICKSEDDNTIATKAIRLM 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIG D+LIEEKWNYGIT+TG +VNE
Sbjct 102 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TINY+TKYMTK D HP F+GKVLCS+GIG GY KR DA H YKKG+TIE YRLRNG+
Sbjct 162 KTINYVTKYMTKIDEKHPDFVGKVLCSRGIGAGYTKRPDAAKHKYKKGETIETYRLRNGA 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEGRRAEQRL 240
KINLPIYYRN+LFTE+ERE+LF+DKIEKGIIYVMGQKVHRD+EE Y+ LL+EGR+ E RL
Sbjct 222 KINLPIYYRNKLFTEKERELLFIDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRL 281
Query 241 YGNHVQEWEKEKYLNrlrkqkkkqereleqlelYWAFEKAKTYTQIESCPF 291
YG ++Q WEK+KYL+RLR+QKKKQ++EL+QLE YWA +AK YT ++ CPF
Sbjct 282 YGYNLQNWEKQKYLSRLRRQKKKQDKELQQLETYWAINRAKKYTSLDECPF 332
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 436 bits (1122), Expect = 5e-156, Method: Compositional matrix adjust.
Identities = 225/291 (77%), Positives = 251/291 (86%), Gaps = 0/291 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
MECRQQKQRQWLVRMSEELRQ PNAYF+TLTI D++Y L N D N IATKAIRL
Sbjct 1 MECRQQKQRQWLVRMSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLC 60
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRK+TGKSIKHWFITELGHEKTERLHLHGIVWGIG DKLI KWNYGITFTG+FVNE
Sbjct 61 LERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNE 120
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TI YITKYMTK D H FIGKVLCSKGIG GY KR+DAK H YK G+TIE YRLRNGS
Sbjct 121 KTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGS 180
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEGRRAEQRL 240
KINLPIYYRNQLFTEEE+E LFLDKIEKGIIYVMGQKVHRD+EE Y+ LL EGR+ E RL
Sbjct 181 KINLPIYYRNQLFTEEEKEALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLNEGRKTECRL 240
Query 241 YGNHVQEWEKEKYLNrlrkqkkkqereleqlelYWAFEKAKTYTQIESCPF 291
YG ++Q WE++KYL+RLR+QKK+Q+++L+QLE YWA E+ K YTQ+E+CPF
Sbjct 241 YGYNLQNWEQQKYLSRLRRQKKRQDKDLQQLETYWALERTKDYTQLETCPF 291
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 436 bits (1122), Expect = 2e-155, Method: Compositional matrix adjust.
Identities = 225/291 (77%), Positives = 252/291 (87%), Gaps = 0/291 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
MECRQQKQRQWLVRMSEELRQ PNAYF+TLTI D++Y L N D N IATKAIRL
Sbjct 42 MECRQQKQRQWLVRMSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLC 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRK+TGKSIKHWFITELGHEKTERLHLHGIVWGIG DKLI KWNYGITFTG+FVNE
Sbjct 102 LERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TI YITKYMTK D H FIGKVLCSKGIG GY KR+DAK H YK+G+TIE YRLRNGS
Sbjct 162 KTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKRGETIETYRLRNGS 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEGRRAEQRL 240
KINLPIYYRNQLFTEEERE LFLDKIEKGIIYVMGQKVHRD+EE Y+ LL+EGR+ E RL
Sbjct 222 KINLPIYYRNQLFTEEEREALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRL 281
Query 241 YGNHVQEWEKEKYLNrlrkqkkkqereleqlelYWAFEKAKTYTQIESCPF 291
YG ++Q WE++KYL+RLR+QKK+Q+++L+QLE YWA EK+K YT ++ CPF
Sbjct 282 YGYNLQNWEQQKYLSRLRRQKKRQDKDLQQLETYWAIEKSKEYTSLDKCPF 332
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 258 bits (659), Expect = 5e-86, Method: Compositional matrix adjust.
Identities = 127/252 (50%), Positives = 173/252 (69%), Gaps = 0/252 (0%)
Query 2 ECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLML 61
ECR+QK R W VR+SEE+R PNA F+TLTISDE++E +KN K +RL L
Sbjct 43 ECRKQKGRAWQVRLSEEVRSDPNAIFVTLTISDESWEKIKNTYIQLSDEDCIKKMVRLFL 102
Query 62 ERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNEQ 121
ER+RKKT KS+KHW TE G TER HLHG++WG + L + W YG F G FVNE
Sbjct 103 ERVRKKTKKSLKHWLTTERGGTNTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNEC 162
Query 122 TINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGSK 181
T+NYITKY+TK D H F LCS GIG GY R D++ + +++GKT E YRLRNG+K
Sbjct 163 TVNYITKYITKTDKKHKDFEPITLCSAGIGKGYLSRSDSELNRFREGKTTETYRLRNGTK 222
Query 182 INLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEGRRAEQRLY 241
+NLPIYYRN+L+T+EERE LFL+KI+KG +++ G++ + + + Y +L+E + ++L+
Sbjct 223 LNLPIYYRNKLYTDEEREKLFLEKIKKGKVWICGRECNIKDWKTYSQILKEEQIKAKQLH 282
Query 242 GNHVQEWEKEKY 253
G+ +W+ KY
Sbjct 283 GDSPIDWDIAKY 294
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 223 bits (567), Expect = 5e-72, Method: Compositional matrix adjust.
Identities = 124/254 (49%), Positives = 167/254 (66%), Gaps = 7/254 (3%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNA-YFMTLTISDENYEILKNICKSEDPNTIATKAIRL 59
+ECRQQKQR W+VR+SEELR A F+TLT ++E+Y+ L I K+E N + A+
Sbjct 42 IECRQQKQRAWIVRLSEELRSGKGAGLFVTLTFNEESYKELAAITKNE--NDMCRLALYR 99
Query 60 MLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVN 119
M E R+K +I+HW +TE+G + R+H+HGI+W D IE W YG + G FVN
Sbjct 100 MNENYRQKYKHTIRHWCVTEIGDDG--RIHIHGIMWCPASD--IERYWKYGYIYIGRFVN 155
Query 120 EQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNG 179
EQTI YITKYM K F KVLCSKGIG+ Y R D+K + Y++ T E Y LR+G
Sbjct 156 EQTILYITKYMLKYTPVDKNFEPKVLCSKGIGINYLDRLDSKRNTYRENNTDESYMLRSG 215
Query 180 SKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEGRRAEQR 239
KINLP YY+ +++TEEERE L+++K EKG Y+MG+KV DNEE+ L+E R+ +
Sbjct 216 RKINLPDYYKRKIYTEEEREKLWIEKQEKGYRYIMGEKVSTDNEEKVYKLMEYWRKKAKE 275
Query 240 LYGNHVQEWEKEKY 253
LY QEW++EK+
Sbjct 276 LYNEKPQEWDREKH 289
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 214 bits (544), Expect = 1e-68, Method: Compositional matrix adjust.
Identities = 118/260 (45%), Positives = 167/260 (64%), Gaps = 12/260 (5%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEIL--------KNICKSEDPNTI 52
+ECR++K R W +R++EEL+ PNA F+TLT ++ENY+ L K+ + N +
Sbjct 40 IECRKEKARNWRIRLAEELKNNPNALFITLTFNEENYQRLSWELFKKSKDNLNYTEQNEM 99
Query 53 ATKAIRLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGIT 112
A+R LERIRKKT KSI+HW +TE G E R+HLHGIVW K E+W YG T
Sbjct 100 CKTAVRRWLERIRKKTKKSIRHWMVTEKG-EDYGRIHLHGIVW---CPKERIEQWGYGYT 155
Query 113 FTGYFVNEQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIE 172
+ G +VNE TI Y+TKYM K P F GKV+CS GIG Y +AK + Y+ +T E
Sbjct 156 YIGDYVNETTIAYVTKYMLKICEKWPDFRGKVMCSAGIGCRYETSYNAKRNRYRGKETKE 215
Query 173 YYRLRNGSKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEE 232
Y+L NG ++ LP YY ++++TEEERE L++ K E+G Y+ G+KV DN EE+ L +
Sbjct 216 TYKLENGRELPLPKYYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTDNLEEWDNLTKY 275
Query 233 GRRAEQRLYGNHVQEWEKEK 252
++ ++LYG+ ++WE+EK
Sbjct 276 YQKRAEQLYGDKPEDWEREK 295
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 208 bits (530), Expect = 7e-66, Method: Compositional matrix adjust.
Identities = 114/255 (45%), Positives = 164/255 (64%), Gaps = 12/255 (5%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICK---SEDPNTIATKAI 57
+ECR++KQR+W VR+ EE+ + YF TLTI EN + +++I E+PN IATKA+
Sbjct 42 IECRKEKQREWRVRLEEEM-HSNFGYFTTLTIDQENIKKIESITGLKWEENPNEIATKAL 100
Query 58 RLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYF 117
RL LER RK TGKSI+HW +TELG EK R+HLHGI +G +LI + WNYG F G +
Sbjct 101 RLFLERTRKDTGKSIRHWCVTELG-EKNNRIHLHGIFFGQKSAELIRKHWNYGFIFIGGY 159
Query 118 VNEQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKRED----AKNHVYKKGKTIEY 173
N ++INYITKYM K D+ HP F VL S GIG GY R D +N+ K +
Sbjct 160 CNSKSINYITKYMLKVDIKHPEFKQIVLASSGIGKGYIDRLDYLWQKQNY---KNINVAT 216
Query 174 YRLRNGSKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNEEEYIALLEEG 233
Y RNG+K+ +P YY+N++FTE+ERE ++++ + +G++++ G+KV D+ E L E
Sbjct 217 YTFRNGTKMAMPKYYKNKIFTEKEREKMWINNLNRGLLWIYGEKVKADDWETIDNLREYW 276
Query 234 RRAEQRLYGNHVQEW 248
++ + G++ W
Sbjct 277 QKYGRETMGDNPIAW 291
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 192 bits (487), Expect = 1e-59, Method: Compositional matrix adjust.
Identities = 111/240 (46%), Positives = 151/240 (63%), Gaps = 9/240 (4%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
ECR++K+ W VR E+LR+TP A F T T+S E Y+ +K + N I TK RL
Sbjct 42 FECRKKKRNAWRVRNFEQLRETPTAIFFTGTVSPERYDYIKEKYNLKTDNEIITKIHRLF 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVW---GIGIDKLI---EEKWNYGITFT 114
LERIRK+TGKS+KHW +TE GH T R+HLHGI + G+ KLI W G +
Sbjct 102 LERIRKETGKSMKHWCVTEKGHTNTRRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYN 161
Query 115 GYFVNEQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYY 174
G + NE+TINY++KYMTK+D+D+P + GKVLCS G+G GY KR K H + + T E Y
Sbjct 162 GKYCNEKTINYVSKYMTKKDMDNPEYTGKVLCSPGLGAGYVKR-IGKRHEWNEENTKEDY 220
Query 175 RLRNGSKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDNE--EEYIALLEE 232
R G+ I LP YY+ +LFTE++RE L++ + G +V KV +E EEY +L++
Sbjct 221 YTRQGTYIALPKYYKYKLFTEDQREQLWIYRENSGEKFVGNFKVKITDEESEEYYNVLKK 280
Lambda K H a alpha
0.319 0.137 0.414 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 24160043