bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-25_CDS_annotation_glimmer3.pl_2_5
Length=238
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 168 1e-51
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 168 1e-51
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 166 4e-51
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 166 1e-50
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 165 2e-50
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 160 6e-49
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 152 8e-46
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 150 6e-45
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 137 1e-39
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 132 6e-38
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 168 bits (426), Expect = 1e-51, Method: Compositional matrix adjust.
Identities = 92/201 (46%), Positives = 129/201 (64%), Gaps = 3/201 (1%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKEP 58
VR+ EE+++ P N F TLT S+E+ + L+ ++ N KAI L +R KK +
Sbjct 54 VRMSEELRTEP-NAYFMTLTISDENYEILKNICKSEDDNTIATKAIRLMLERIRKKTGKS 112
Query 59 LKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIKYIT 118
+KHW ITELGH+ T+R+HLHGI+W T++ E++W YG + G VNE+TINY+ KY+T
Sbjct 113 IKHWFITELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNEKTINYVTKYMT 172
Query 119 KRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALPTYYKQK 178
K DE +P+F GK+ S+GIG Y +H+Y+ T ETYR +G KI LP YY+ K
Sbjct 173 KIDEKHPDFVGKVLCSRGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNK 232
Query 179 LWTVQEREALRIIKEEKQVKY 199
L+T +ERE L I K EK + Y
Sbjct 233 LFTEKERELLFIDKIEKGIIY 253
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 168 bits (425), Expect = 1e-51, Method: Compositional matrix adjust.
Identities = 89/201 (44%), Positives = 126/201 (63%), Gaps = 3/201 (1%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKEP 58
VR+ EE++ NP N F TLT +E+ KL + K+ N+ KA+ L +R KK +
Sbjct 54 VRMSEELRQNP-NAYFMTLTIDDENYNKLANICNSKDNNEIATKAVRLMLERIRKKTGKS 112
Query 59 LKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIKYIT 118
+KHW ITELGH+ T+R+HLHGI+W T++ ++W YG+++ G VNE TINYI KY+T
Sbjct 113 IKHWFITELGHEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNEATINYITKYMT 172
Query 119 KRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALPTYYKQK 178
K D +P+F G++ SKGIG Y H+Y T ETYR +G KI LP YY+ +
Sbjct 173 KVDIDHPDFVGQVLCSKGIGAGYTKREDANNHKYTKGKTNETYRLRNGAKINLPIYYRNQ 232
Query 179 LWTVQEREALRIIKEEKQVKY 199
L++ +ERE L + K EK + Y
Sbjct 233 LFSEEEREMLFLDKIEKGIIY 253
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 166 bits (419), Expect = 4e-51, Method: Compositional matrix adjust.
Identities = 93/220 (42%), Positives = 135/220 (61%), Gaps = 3/220 (1%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKEP 58
VR+ EE++ P N F TLT ++S +L Y+ + N+ KAI L +R K+ +
Sbjct 13 VRMSEELRQEP-NAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKS 71
Query 59 LKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIKYIT 118
+KHW ITELGH+ T+R+HLHGI+W T++ +W YG F G+ VNE+TI YI KY+T
Sbjct 72 IKHWFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNEKTIQYITKYMT 131
Query 119 KRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALPTYYKQK 178
K DE + +F GK+ SKGIG YI + ++H Y+ T ETYR +G KI LP YY+ +
Sbjct 132 KIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGSKINLPIYYRNQ 191
Query 179 LWTVQEREALRIIKEEKQVKYYNKTPIRVETIEQYREYVN 218
L+T +E+EAL + K EK + Y + + E Y + +N
Sbjct 192 LFTEEEKEALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLN 231
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 166 bits (419), Expect = 1e-50, Method: Compositional matrix adjust.
Identities = 91/201 (45%), Positives = 128/201 (64%), Gaps = 3/201 (1%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKEP 58
VR+ EE++ P N F TLT ++S +L Y+ + N+ KAI L +R K+ +
Sbjct 54 VRMSEELRQEP-NAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKS 112
Query 59 LKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIKYIT 118
+KHW ITELGH+ T+R+HLHGI+W T++ +W YG F G+ VNE+TI YI KY+T
Sbjct 113 IKHWFITELGHEKTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNEKTIQYITKYMT 172
Query 119 KRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALPTYYKQK 178
K DE + +F GK+ SKGIG YI + ++H Y+ T ETYR +G KI LP YY+ +
Sbjct 173 KIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKRGETIETYRLRNGSKINLPIYYRNQ 232
Query 179 LWTVQEREALRIIKEEKQVKY 199
L+T +EREAL + K EK + Y
Sbjct 233 LFTEEEREALFLDKIEKGIIY 253
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 165 bits (418), Expect = 2e-50, Method: Compositional matrix adjust.
Identities = 95/208 (46%), Positives = 129/208 (62%), Gaps = 5/208 (2%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEES---LKKLEYDEKEPNKAPQKAISLFRKRWWKKYKE 57
VR+ EE+++ P N F TLT S+E+ LK + E E N KAI L +R KK +
Sbjct 54 VRMSEELRTEP-NAYFMTLTISDENYEILKNICKSEDE-NTIATKAIRLMLERIRKKIGK 111
Query 58 PLKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIKYI 117
++HW ITELGH+ T+R+HLHGI+W T++ ++W YG + G VNE+TINYI KY+
Sbjct 112 SIRHWFITELGHEKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNEKTINYITKYM 171
Query 118 TKRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALPTYYKQ 177
TK DE +P F GK+ SKGIG Y +H+Y+ T ETYR +G KI LP YY+
Sbjct 172 TKIDEEHPNFVGKVLCSKGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRN 231
Query 178 KLWTVQEREALRIIKEEKQVKYYNKTPI 205
KL+T +ERE L I K +K + Y T +
Sbjct 232 KLFTEKERELLFIDKIDKGIIYVLGTKV 259
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 160 bits (405), Expect = 6e-49, Method: Compositional matrix adjust.
Identities = 89/190 (47%), Positives = 121/190 (64%), Gaps = 3/190 (2%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKEP 58
VRL EE++S+P N IF TLT S+ES +K++ Y + +K + LF +R KK K+
Sbjct 54 VRLSEEVRSDP-NAIFVTLTISDESWEKIKNTYIQLSDEDCIKKMVRLFLERVRKKTKKS 112
Query 59 LKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIKYIT 118
LKHWL TE G NT+R HLHG+IW E TE + W YG++F G VNE T+NYI KYIT
Sbjct 113 LKHWLTTERGGTNTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNECTVNYITKYIT 172
Query 119 KRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALPTYYKQK 178
K D+ + +F S GIGK Y+ + +R+++ T ETYR +G K+ LP YY+ K
Sbjct 173 KTDKKHKDFEPITLCSAGIGKGYLSRSDSELNRFREGKTTETYRLRNGTKLNLPIYYRNK 232
Query 179 LWTVQEREAL 188
L+T +ERE L
Sbjct 233 LYTDEEREKL 242
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 152 bits (385), Expect = 8e-46, Method: Compositional matrix adjust.
Identities = 92/234 (39%), Positives = 143/234 (61%), Gaps = 18/234 (8%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLEY----------DEKEPNKAPQKAISLFRKR 50
+RL EE+K+NP N +F TLTF+EE+ ++L + + E N+ + A+ + +R
Sbjct 52 IRLAEELKNNP-NALFITLTFNEENYQRLSWELFKKSKDNLNYTEQNEMCKTAVRRWLER 110
Query 51 WWKKYKEPLKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTI 110
KK K+ ++HW++TE G D RIHLHGI+W +E+ E +WGYG+ + G VNE TI
Sbjct 111 IRKKTKKSIRHWMVTEKGED-YGRIHLHGIVW--CPKERIE-QWGYGYTYIGDYVNETTI 166
Query 111 NYIIKYITKRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIA 170
Y+ KY+ K E P+F GK+ S GIG Y + +R+RY+ + T+ETY+ +G ++
Sbjct 167 AYVTKYMLKICEKWPDFRGKVMCSAGIGCRYETSYNAKRNRYRGKETKETYKLENGRELP 226
Query 171 LPTYYKQKLWTVQEREALRIIKEEKQVKYYNKTPIRVETIEQYREYVNAVKYWQ 224
LP YY K++T +ERE L IIK+E+ +Y + + +E E+ N KY+Q
Sbjct 227 LPKYYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTDNLE---EWDNLTKYYQ 277
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 150 bits (379), Expect = 6e-45, Method: Compositional matrix adjust.
Identities = 84/199 (42%), Positives = 122/199 (61%), Gaps = 4/199 (2%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLEYDEKEPNKAPQKAISLFRKRWWKKYKEPLK 60
VRL EE++S +F TLTF+EES K+L K N + A+ + + +KYK ++
Sbjct 54 VRLSEELRSGKGAGLFVTLTFNEESYKELAAITKNENDMCRLALYRMNENYRQKYKHTIR 113
Query 61 HWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIKYITKR 120
HW +TE+G D RIH+HGI+W ++ E+ W YG+I+ G VNE+TI YI KY+ K
Sbjct 114 HWCVTEIGDDG--RIHIHGIMWCPASD--IERYWKYGYIYIGRFVNEQTILYITKYMLKY 169
Query 121 DEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALPTYYKQKLW 180
+ F K+ SKGIG Y+ +R+ Y++ T+E+Y SG KI LP YYK+K++
Sbjct 170 TPVDKNFEPKVLCSKGIGINYLDRLDSKRNTYRENNTDESYMLRSGRKINLPDYYKRKIY 229
Query 181 TVQEREALRIIKEEKQVKY 199
T +ERE L I K+EK +Y
Sbjct 230 TEEEREKLWIEKQEKGYRY 248
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 137 bits (345), Expect = 1e-39, Method: Compositional matrix adjust.
Identities = 89/229 (39%), Positives = 126/229 (55%), Gaps = 11/229 (5%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLK--KLEYDEKEPNKAPQKAISLFRKRWWKKYKEP 58
VR E+++ P I F T T S E K +Y+ K N+ K LF +R K+ +
Sbjct 54 VRNFEQLRETPTAIFF-TGTVSPERYDYIKEKYNLKTDNEIITKIHRLFLERIRKETGKS 112
Query 59 LKHWLITELGHDNTKRIHLHGIIWTELTEEQFE------KEWGYGWIFFGYEVNERTINY 112
+KHW +TE GH NT+RIHLHGI + QF+ W G+ + G NE+TINY
Sbjct 113 MKHWCVTEKGHTNTRRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYNGKYCNEKTINY 172
Query 113 IIKYITKRDEANPEFNGKIFTSKGIGKEYIGENSLRRHRYQDRFTEETYRTNSGIKIALP 172
+ KY+TK+D NPE+ GK+ S G+G Y+ +RH + + T+E Y T G IALP
Sbjct 173 VSKYMTKKDMDNPEYTGKVLCSPGLGAGYVKRIG-KRHEWNEENTKEDYYTRQGTYIALP 231
Query 173 TYYKQKLWTVQEREALRIIKEEKQVKYYNKTPIRVETIEQYREYVNAVK 221
YYK KL+T +RE L I +E K+ +++ T E+ EY N +K
Sbjct 232 KYYKYKLFTEDQREQLWIYRENSGEKFVGNFKVKI-TDEESEEYYNVLK 279
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 132 bits (333), Expect = 6e-38, Method: Compositional matrix adjust.
Identities = 87/225 (39%), Positives = 125/225 (56%), Gaps = 12/225 (5%)
Query 1 VRLMEEIKSNPKNIIFATLTFSEESLKKLEYD-----EKEPNKAPQKAISLFRKRWWKKY 55
VRL EE+ SN F TLT +E++KK+E E+ PN+ KA+ LF +R K
Sbjct 54 VRLEEEMHSNFG--YFTTLTIDQENIKKIESITGLKWEENPNEIATKALRLFLERTRKDT 111
Query 56 KEPLKHWLITELGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVNERTINYIIK 115
+ ++HW +TELG N RIHLHGI + + + E K W YG+IF G N ++INYI K
Sbjct 112 GKSIRHWCVTELGEKNN-RIHLHGIFFGQKSAELIRKHWNYGFIFIGGYCNSKSINYITK 170
Query 116 YITKRDEANPEFNGKIFTSKGIGKEYIGE-NSLRRHRYQDRFTEETYRTNSGIKIALPTY 174
Y+ K D +PEF + S GIGK YI + L + + TY +G K+A+P Y
Sbjct 171 YMLKVDIKHPEFKQIVLASSGIGKGYIDRLDYLWQKQNYKNINVATYTFRNGTKMAMPKY 230
Query 175 YKQKLWTVQEREALRIIKEEKQVKYYNKTPIRV---ETIEQYREY 216
YK K++T +ERE + I + + + ++ ETI+ REY
Sbjct 231 YKNKIFTEKEREKMWINNLNRGLLWIYGEKVKADDWETIDNLREY 275
Lambda K H a alpha
0.316 0.135 0.409 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 18832452