bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-26_CDS_annotation_glimmer3.pl_2_4
Length=255
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 162 3e-49
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 161 9e-49
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 160 3e-48
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 158 5e-48
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 159 7e-48
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 153 7e-46
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 142 1e-41
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 140 3e-41
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 133 4e-38
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 129 2e-36
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 162 bits (411), Expect = 3e-49, Method: Compositional matrix adjust.
Identities = 86/189 (46%), Positives = 122/189 (65%), Gaps = 2/189 (1%)
Query 15 NIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGHD 72
N F TLT S+E+ + L+ ++ N KAI L +R KK +KHW ITE+GH+
Sbjct 65 NAYFMTLTISDENYEILKNICKSEDDNTIATKAIRLMLERIRKKTGKSIKHWFITELGHE 124
Query 73 NTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFNGK 132
T+R+HLHGI+W T++ E++W YG + G V+EKTINY+ KY+TK DE +P+F GK
Sbjct 125 KTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNEKTINYVTKYMTKIDEKHPDFVGK 184
Query 133 IFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESLRI 192
+ S+GIG GY + KH+Y+ T ETYR +G K+ LP YY+ KL+T++ERE L I
Sbjct 185 VLCSRGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKERELLFI 244
Query 193 IKEEQQVKY 201
K E+ + Y
Sbjct 245 DKIEKGIIY 253
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 161 bits (407), Expect = 9e-49, Method: Compositional matrix adjust.
Identities = 81/191 (42%), Positives = 121/191 (63%), Gaps = 2/191 (1%)
Query 13 SKNIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMG 70
+ N F TLT +E+ KL + K+ N+ KA+ L +R KK +KHW ITE+G
Sbjct 63 NPNAYFMTLTIDDENYNKLANICNSKDNNEIATKAVRLMLERIRKKTGKSIKHWFITELG 122
Query 71 HDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFN 130
H+ T+R+HLHGI+W T++ ++W YG+++ G V+E TINYI KY+TK D +P+F
Sbjct 123 HEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNEATINYITKYMTKVDIDHPDFV 182
Query 131 GKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESL 190
G++ SKGIG GY + N H+Y T ETYR +G K+ LP YY+ +L++++ERE L
Sbjct 183 GQVLCSKGIGAGYTKREDANNHKYTKGKTNETYRLRNGAKINLPIYYRNQLFSEEEREML 242
Query 191 RIIKEEQQVKY 201
+ K E+ + Y
Sbjct 243 FLDKIEKGIIY 253
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 160 bits (404), Expect = 3e-48, Method: Compositional matrix adjust.
Identities = 89/196 (45%), Positives = 122/196 (62%), Gaps = 4/196 (2%)
Query 15 NIIFATLTFSEES---LKKLEYDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGH 71
N F TLT S+E+ LK + E E N KAI L +R KK ++HW ITE+GH
Sbjct 65 NAYFMTLTISDENYEILKNICKSEDE-NTIATKAIRLMLERIRKKIGKSIRHWFITELGH 123
Query 72 DNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFNG 131
+ T+R+HLHGI+W T++ ++W YG + G V+EKTINYI KY+TK DE +P F G
Sbjct 124 EKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNEKTINYITKYMTKIDEEHPNFVG 183
Query 132 KIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESLR 191
K+ SKGIG GY + KH+Y+ T ETYR +G K+ LP YY+ KL+T++ERE L
Sbjct 184 KVLCSKGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKERELLF 243
Query 192 IIKEEQQVKYYNKTPI 207
I K ++ + Y T +
Sbjct 244 IDKIDKGIIYVLGTKV 259
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 158 bits (400), Expect = 5e-48, Method: Compositional matrix adjust.
Identities = 86/208 (41%), Positives = 128/208 (62%), Gaps = 2/208 (1%)
Query 15 NIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGHD 72
N F TLT ++S +L Y+ + N+ KAI L +R K+ +KHW ITE+GH+
Sbjct 24 NAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKSIKHWFITELGHE 83
Query 73 NTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFNGK 132
T+R+HLHGI+W T++ +W YG F G+ V+EKTI YI KY+TK DE + +F GK
Sbjct 84 KTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNEKTIQYITKYMTKIDEQHKDFIGK 143
Query 133 IFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESLRI 192
+ SKGIG GYI ++ KH Y+ T ETYR +G K+ LP YY+ +L+T++E+E+L +
Sbjct 144 VLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGSKINLPIYYRNQLFTEEEKEALFL 203
Query 193 IKEEQQVKYYNKTPIKVETLEQYKEYVN 220
K E+ + Y + + E Y + +N
Sbjct 204 DKIEKGIIYVMGQKVHRDDEEYYLQLLN 231
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 159 bits (402), Expect = 7e-48, Method: Compositional matrix adjust.
Identities = 84/189 (44%), Positives = 121/189 (64%), Gaps = 2/189 (1%)
Query 15 NIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGHD 72
N F TLT ++S +L Y+ + N+ KAI L +R K+ +KHW ITE+GH+
Sbjct 65 NAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKSIKHWFITELGHE 124
Query 73 NTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFNGK 132
T+R+HLHGI+W T++ +W YG F G+ V+EKTI YI KY+TK DE + +F GK
Sbjct 125 KTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNEKTIQYITKYMTKIDEQHKDFIGK 184
Query 133 IFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESLRI 192
+ SKGIG GYI ++ KH Y+ T ETYR +G K+ LP YY+ +L+T++ERE+L +
Sbjct 185 VLCSKGIGAGYIKRDDAKKHTYKRGETIETYRLRNGSKINLPIYYRNQLFTEEEREALFL 244
Query 193 IKEEQQVKY 201
K E+ + Y
Sbjct 245 DKIEKGIIY 253
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 153 bits (386), Expect = 7e-46, Method: Compositional matrix adjust.
Identities = 82/178 (46%), Positives = 113/178 (63%), Gaps = 2/178 (1%)
Query 15 NIIFATLTFSEESLKKLE--YDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGHD 72
N IF TLT S+ES +K++ Y + +K + LF +R KK K LKHWL TE G
Sbjct 65 NAIFVTLTISDESWEKIKNTYIQLSDEDCIKKMVRLFLERVRKKTKKSLKHWLTTERGGT 124
Query 73 NTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFNGK 132
NT+R HLHG+IW E TE + W YG++F G V+E T+NYI KY+TK D+ + +F
Sbjct 125 NTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNECTVNYITKYITKTDKKHKDFEPI 184
Query 133 IFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESL 190
S GIG GY++++ +R+++ T ETYR +G KL LP YY+ KL+TD+ERE L
Sbjct 185 TLCSAGIGKGYLSRSDSELNRFREGKTTETYRLRNGTKLNLPIYYRNKLYTDEEREKL 242
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 142 bits (358), Expect = 1e-41, Method: Compositional matrix adjust.
Identities = 75/185 (41%), Positives = 117/185 (63%), Gaps = 4/185 (2%)
Query 17 IFATLTFSEESLKKLEYDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGHDNTKR 76
+F TLTF+EES K+L K N + A+ + + +KYK ++HW +TE+G D R
Sbjct 68 LFVTLTFNEESYKELAAITKNENDMCRLALYRMNENYRQKYKHTIRHWCVTEIGDDG--R 125
Query 77 IHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFNGKIFTS 136
IH+HGI+W ++ E+ W YG+I+ G V+E+TI YI KY+ K + F K+ S
Sbjct 126 IHIHGIMWCPASD--IERYWKYGYIYIGRFVNEQTILYITKYMLKYTPVDKNFEPKVLCS 183
Query 137 KGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESLRIIKEE 196
KGIGI Y+++ ++ Y++ T+E+Y SG K+ LP YYK+K++T++ERE L I K+E
Sbjct 184 KGIGINYLDRLDSKRNTYRENNTDESYMLRSGRKINLPDYYKRKIYTEEEREKLWIEKQE 243
Query 197 QQVKY 201
+ +Y
Sbjct 244 KGYRY 248
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 140 bits (354), Expect = 3e-41, Method: Compositional matrix adjust.
Identities = 85/222 (38%), Positives = 131/222 (59%), Gaps = 17/222 (8%)
Query 15 NIIFATLTFSEESLKKLEY----------DEKEPNKAPQKAISLFRKRWWKKYKTPLKHW 64
N +F TLTF+EE+ ++L + + E N+ + A+ + +R KK K ++HW
Sbjct 63 NALFITLTFNEENYQRLSWELFKKSKDNLNYTEQNEMCKTAVRRWLERIRKKTKKSIRHW 122
Query 65 LITEMGHDNTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDE 124
++TE G D RIHLHGI+W +E+ E +WGYG+ + G V+E TI Y+ KY+ K E
Sbjct 123 MVTEKGEDYG-RIHLHGIVWC--PKERIE-QWGYGYTYIGDYVNETTIAYVTKYMLKICE 178
Query 125 ANPEFNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTD 184
P+F GK+ S GIG Y + ++RY+ K T+ETY+ +G +L LP YY K++T+
Sbjct 179 KWPDFRGKVMCSAGIGCRYETSYNAKRNRYRGKETKETYKLENGRELPLPKYYHDKIYTE 238
Query 185 QERESLRIIKEEQQVKYYNKTPIKVETLEQYKEYVNAVMYWQ 226
+ERE L IIK+E+ +Y + + LE E+ N Y+Q
Sbjct 239 EEREKLWIIKQERGYRYIAGEKVSTDNLE---EWDNLTKYYQ 277
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 133 bits (335), Expect = 4e-38, Method: Compositional matrix adjust.
Identities = 84/214 (39%), Positives = 120/214 (56%), Gaps = 10/214 (5%)
Query 17 IFATLTFSEESLK--KLEYDEKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGHDNT 74
IF T T S E K +Y+ K N+ K LF +R K+ +KHW +TE GH NT
Sbjct 67 IFFTGTVSPERYDYIKEKYNLKTDNEIITKIHRLFLERIRKETGKSMKHWCVTEKGHTNT 126
Query 75 KRIHLHGIIWTELTEEQFE------KEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPE 128
+RIHLHGI + QF+ W G+ + G +EKTINY+ KY+TK+D NPE
Sbjct 127 RRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYNGKYCNEKTINYVSKYMTKKDMDNPE 186
Query 129 FNGKIFTSKGIGIGYINKNSLNKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERE 188
+ GK+ S G+G GY+ K +H + ++ T+E Y G +ALP YYK KL+T+ +RE
Sbjct 187 YTGKVLCSPGLGAGYV-KRIGKRHEWNEENTKEDYYTRQGTYIALPKYYKYKLFTEDQRE 245
Query 189 SLRIIKEEQQVKYYNKTPIKVETLEQYKEYVNAV 222
L I +E K+ +K+ T E+ +EY N +
Sbjct 246 QLWIYRENSGEKFVGNFKVKI-TDEESEEYYNVL 278
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 129 bits (323), Expect = 2e-36, Method: Compositional matrix adjust.
Identities = 79/211 (37%), Positives = 119/211 (56%), Gaps = 12/211 (6%)
Query 18 FATLTFSEESLKKLEYD-----EKEPNKAPQKAISLFRKRWWKKYKTPLKHWLITEMGHD 72
F TLT +E++KK+E E+ PN+ KA+ LF +R K ++HW +TE+G
Sbjct 67 FTTLTIDQENIKKIESITGLKWEENPNEIATKALRLFLERTRKDTGKSIRHWCVTELGEK 126
Query 73 NTKRIHLHGIIWTELTEEQFEKEWGYGWIFFGYEVSEKTINYIVKYVTKRDEANPEFNGK 132
N RIHLHGI + + + E K W YG+IF G + K+INYI KY+ K D +PEF
Sbjct 127 NN-RIHLHGIFFGQKSAELIRKHWNYGFIFIGGYCNSKSINYITKYMLKVDIKHPEFKQI 185
Query 133 IFTSKGIGIGYINKNSL--NKHRYQDKFTEETYRAASGIKLALPTYYKQKLWTDQERESL 190
+ S GIG GYI++ K Y++ TY +G K+A+P YYK K++T++ERE +
Sbjct 186 VLASSGIGKGYIDRLDYLWQKQNYKN-INVATYTFRNGTKMAMPKYYKNKIFTEKEREKM 244
Query 191 RIIKEEQQVKYYNKTPIKV---ETLEQYKEY 218
I + + + +K ET++ +EY
Sbjct 245 WINNLNRGLLWIYGEKVKADDWETIDNLREY 275
Lambda K H a alpha
0.315 0.132 0.398 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 20476208