bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-32_CDS_annotation_glimmer3.pl_2_2
Length=104
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4 98.6 8e-27
Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4 97.4 2e-26
Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4 28.5 0.010
Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4 26.6 0.048
Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4 24.6 0.17
Alpavirinae_Human_feces_A_048_Microviridae_AG087_putative.VP1 23.5 0.46
Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4 23.5 0.48
Alpavirinae_Human_feces_B_021_Microviridae_AG0368_putative.VP1 23.1 0.62
Alpavirinae_Human_gut_33_017_Microviridae_AG0152_putative.VP4 22.7 0.99
Microvirus_gi|9625365|ref|NP_039599.1|_minor_spike_protein_[Ent... 22.3 1.2
> Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4
Length=532
Score = 98.6 bits (244), Expect = 8e-27, Method: Compositional matrix adjust.
Identities = 44/103 (43%), Positives = 73/103 (71%), Gaps = 0/103 (0%)
Query 1 MGFSLFSPRVDYNRIHSAIKLSKEFFDVREKQSLQNLFSDSQSFESDWSDIFWNQTEERQ 60
+G++L S RV+Y I +++ S F++ RE++SLQ+LF DS++FESDWSDIFW++ +E+
Sbjct 426 IGYTLLSTRVEYLLIKKSLENSINFYNERERKSLQDLFHDSEAFESDWSDIFWDRRQEKI 485
Query 61 REFAESGLGSACRSELSALVHSKVKHRHLNDMNNFFTERANYN 103
+ F +S GS CR +L + + ++KHR +ND FT+++ N
Sbjct 486 KRFVDSDYGSLCRDKLHSEIRKRIKHREINDAVGIFTKQSYRN 528
> Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4
Length=532
Score = 97.4 bits (241), Expect = 2e-26, Method: Compositional matrix adjust.
Identities = 43/103 (42%), Positives = 73/103 (71%), Gaps = 0/103 (0%)
Query 1 MGFSLFSPRVDYNRIHSAIKLSKEFFDVREKQSLQNLFSDSQSFESDWSDIFWNQTEERQ 60
+G++L S RV+Y+ I +++ S F++ RE++SLQ+LF DS++FESDWSDIFW++ +E+
Sbjct 426 IGYTLLSTRVEYSLIKKSLENSINFYNERERKSLQDLFHDSEAFESDWSDIFWDRRQEKI 485
Query 61 REFAESGLGSACRSELSALVHSKVKHRHLNDMNNFFTERANYN 103
+ F +S G+ CR +L + ++KHR +ND FT+++ N
Sbjct 486 KRFVDSDYGNLCRDKLHNEIRKRIKHREINDAVGIFTKQSYRN 528
> Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4
Length=546
Score = 28.5 bits (62), Expect = 0.010, Method: Compositional matrix adjust.
Identities = 20/88 (23%), Positives = 38/88 (43%), Gaps = 4/88 (5%)
Query 9 RVDYNRIHSAIKL---SKEFFDVREKQSLQNLFSDSQSFESDWSDIFWNQTEERQREFAE 65
++D H I+L +E++ + + Q L+ F + E D D + + TE
Sbjct 436 KIDKMPYHEGIRLLDMCREYYRLLDYQYLRQRFQFLEQCEEDLLDFYLHPTENFADYLPR 495
Query 66 SGLGSACRSELSALVHSKVKHRHLNDMN 93
+ + + L +KHR +ND+N
Sbjct 496 TIVTGINQYSLER-YQKAIKHREINDLN 522
> Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4
Length=536
Score = 26.6 bits (57), Expect = 0.048, Method: Compositional matrix adjust.
Identities = 12/30 (40%), Positives = 20/30 (67%), Gaps = 3/30 (10%)
Query 67 GLGSACRS---ELSALVHSKVKHRHLNDMN 93
L S CR+ E++A ++KH++LND+N
Sbjct 497 ALSSFCRAAYAEVAADNRQRIKHKYLNDLN 526
> Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4
Length=559
Score = 24.6 bits (52), Expect = 0.17, Method: Compositional matrix adjust.
Identities = 23/91 (25%), Positives = 37/91 (41%), Gaps = 8/91 (9%)
Query 13 NRIHSAIKLSKEFFDVREKQSLQNLFSDSQSFESDWSDIFWNQTEERQREFAESGLGSAC 72
RI IK E+ ++ +SL+++F + +S + SD + Q S +
Sbjct 466 GRIMFIIKTGIEYEKKKDYESLRDVF-NIRSANPNISDCMFALPANGQERDVLSDVSCET 524
Query 73 RSELSAL-------VHSKVKHRHLNDMNNFF 96
L L +KH+ LND NN F
Sbjct 525 VQLLEQLRLRSATFCRDMIKHKRLNDANNIF 555
> Alpavirinae_Human_feces_A_048_Microviridae_AG087_putative.VP1
Length=650
Score = 23.5 bits (49), Expect = 0.46, Method: Composition-based stats.
Identities = 17/74 (23%), Positives = 33/74 (45%), Gaps = 14/74 (19%)
Query 1 MGFSLFSPRVDYNRIHSAIKLSKEFFDVREKQSLQNLFS---DSQSFES--DWSDIFWNQ 55
MG +PR+DY++ EFF+ E Q++ ++ D ++ +W +W+
Sbjct 491 MGIGSITPRIDYSQ-------GNEFFN--ELQTVDDIHKPALDGIGYQDSLNWQRAWWDD 541
Query 56 TEERQREFAESGLG 69
T + +S G
Sbjct 542 TRMQDNGRIQSSAG 555
> Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4
Length=547
Score = 23.5 bits (49), Expect = 0.48, Method: Compositional matrix adjust.
Identities = 10/29 (34%), Positives = 15/29 (52%), Gaps = 0/29 (0%)
Query 68 LGSACRSELSALVHSKVKHRHLNDMNNFF 96
L A E + + +KH+ LND+N F
Sbjct 516 LVQAVDEECHCRIQNSIKHKELNDLNYCF 544
> Alpavirinae_Human_feces_B_021_Microviridae_AG0368_putative.VP1
Length=649
Score = 23.1 bits (48), Expect = 0.62, Method: Composition-based stats.
Identities = 14/51 (27%), Positives = 26/51 (51%), Gaps = 2/51 (4%)
Query 1 MGFSLFSPRVDYNRIHSAIKLSKEFFDVREKQSLQNL-FSDSQSFESDWSD 50
MG +PR+DY++ + K D+ K +L + + DS +++ W D
Sbjct 490 MGIGSITPRIDYSQGNEFYNELKTVDDI-HKPALDGIGYQDSLNWQRAWWD 539
> Alpavirinae_Human_gut_33_017_Microviridae_AG0152_putative.VP4
Length=565
Score = 22.7 bits (47), Expect = 0.99, Method: Compositional matrix adjust.
Identities = 8/15 (53%), Positives = 11/15 (73%), Gaps = 0/15 (0%)
Query 83 KVKHRHLNDMNNFFT 97
KVKH+ +NDM F+
Sbjct 548 KVKHKKINDMFGIFS 562
> Microvirus_gi|9625365|ref|NP_039599.1|_minor_spike_protein_[Enterobacteria_phage_alpha3]
Length=330
Score = 22.3 bits (46), Expect = 1.2, Method: Composition-based stats.
Identities = 8/23 (35%), Positives = 13/23 (57%), Gaps = 0/23 (0%)
Query 32 QSLQNLFSDSQSFESDWSDIFWN 54
+ + N+ +D+ S SDW WN
Sbjct 291 KDVTNMITDAASGASDWVSQQWN 313
Lambda K H a alpha
0.319 0.130 0.382 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 5389302