bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-33_CDS_annotation_glimmer3.pl_2_3
Length=113
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4 186 2e-58
Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4 35.0 5e-05
Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4 28.1 0.016
Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4 26.9 0.038
Alpavirinae_Human_feces_D_008_Microviridae_AG098_putative.VP1 25.4 0.13
Alpavirinae_Human_feces_D_031_Microviridae_AG0425_putative.VP1 25.4 0.15
Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4 23.1 0.83
Alpavirinae_Human_gut_31_037_Microviridae_AG0294_putative.VP2 21.9 1.7
Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4 21.9 1.8
Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4 21.6 2.5
> Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4
Length=563
Score = 186 bits (472), Expect = 2e-58, Method: Compositional matrix adjust.
Identities = 88/113 (78%), Positives = 100/113 (88%), Gaps = 0/113 (0%)
Query 1 LDWSVPVGKISRFFYRFNRFDKMKGSLRSKLKAVALFYDYRDYESLKNQLSLQELVFSEL 60
L+W +P GKISRF YRFN F+KMKGS RSKLKAV LFYDYRDY+SLKNQL +Q+LVFSEL
Sbjct 451 LNWDIPKGKISRFLYRFNMFEKMKGSFRSKLKAVELFYDYRDYQSLKNQLYMQQLVFSEL 510
Query 61 GYSDELFDSFYIKPCIKKLKNSYTDKWKDTNYREVHYFRVKHKVLNDENNIFL 113
GYSDEL DSFY+KP +K LKN YT+KW DTNY EVHYFRVKHKVLND+N++FL
Sbjct 511 GYSDELLDSFYVKPNLKVLKNIYTEKWHDTNYHEVHYFRVKHKVLNDQNDVFL 563
> Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4
Length=539
Score = 35.0 bits (79), Expect = 5e-05, Method: Compositional matrix adjust.
Identities = 35/135 (26%), Positives = 55/135 (41%), Gaps = 47/135 (35%)
Query 2 DW-SVPVGKISRFFYRFNRFDKMKGSLRSKLKAVALFYDYRDYESLKNQL---------- 50
+W + PVG ++ G L ++A+ FYDY SL +QL
Sbjct 421 NWITAPVGSVA-----------FTGQLHRAVRAIHSFYDYCSRRSLHDQLVKVEKWSNDS 469
Query 51 ------------SLQELVFSELGYSDELFDSFYIKPCIKKLKNSYTDKWKDTNYREVHYF 98
L ++ +S+ +FDS + L+ SY D D RE
Sbjct 470 YVRANLSIYYFYPLTDVDIMMKSFSEIVFDS-------QVLRASYADYAADN--RE---- 516
Query 99 RVKHKVLNDENNIFL 113
R+KHK LND+N++ +
Sbjct 517 RIKHKALNDKNSLLI 531
> Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4
Length=536
Score = 28.1 bits (61), Expect = 0.016, Method: Compositional matrix adjust.
Identities = 26/99 (26%), Positives = 44/99 (44%), Gaps = 11/99 (11%)
Query 25 GSLRSKLKAVALFYDYRDYESLKNQLSLQELVFSELGYSDELFDSFYIKPCI--KKLKNS 82
G LR + + FY Y D + L +QL ++ +S+ Y D Y P + +++S
Sbjct 433 GQLRKAFRTIYAFYSYMDSKYLHDQL-VKVQSWSQNNYLSTQVDFRYFYPLTDGELMRSS 491
Query 83 YTDKWKDTNYREVHYF--------RVKHKVLNDENNIFL 113
Y + +++ Y R+KHK LND N +
Sbjct 492 YREVLALSSFCRAAYAEVAADNRQRIKHKYLNDLNCALI 530
> Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4
Length=559
Score = 26.9 bits (58), Expect = 0.038, Method: Compositional matrix adjust.
Identities = 23/81 (28%), Positives = 37/81 (46%), Gaps = 16/81 (20%)
Query 41 RDYESLKNQLSLQELVFSELGYSDELFDSFYIKPCIKKLKNSYTDKWKDT-------NYR 93
+DYESL++ VF+ + + D + P + ++ +D +T R
Sbjct 482 KDYESLRD-------VFNIRSANPNISDCMFALPANGQERDVLSDVSCETVQLLEQLRLR 534
Query 94 EVHYFR--VKHKVLNDENNIF 112
+ R +KHK LND NNIF
Sbjct 535 SATFCRDMIKHKRLNDANNIF 555
> Alpavirinae_Human_feces_D_008_Microviridae_AG098_putative.VP1
Length=618
Score = 25.4 bits (54), Expect = 0.13, Method: Composition-based stats.
Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 0/26 (0%)
Query 7 VGKISRFFYRFNRFDKMKGSLRSKLK 32
+G + RFF R+D++ G+ RS LK
Sbjct 514 MGYLPRFFDVKTRYDEVLGAFRSTLK 539
> Alpavirinae_Human_feces_D_031_Microviridae_AG0425_putative.VP1
Length=614
Score = 25.4 bits (54), Expect = 0.15, Method: Composition-based stats.
Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 0/26 (0%)
Query 7 VGKISRFFYRFNRFDKMKGSLRSKLK 32
+G + RFF R+D++ G+ RS LK
Sbjct 510 MGYLPRFFDVKTRYDEVLGAFRSTLK 535
> Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4
Length=562
Score = 23.1 bits (48), Expect = 0.83, Method: Compositional matrix adjust.
Identities = 13/40 (33%), Positives = 21/40 (53%), Gaps = 9/40 (23%)
Query 74 PCIKKLKNSYTDKWKDTNYREVHYFRVKHKVLNDENNIFL 113
P +++L + K +D +VKHK +ND + IFL
Sbjct 532 PLVRRLAAASLMKCRD---------KVKHKKVNDLSGIFL 562
> Alpavirinae_Human_gut_31_037_Microviridae_AG0294_putative.VP2
Length=360
Score = 21.9 bits (45), Expect = 1.7, Method: Composition-based stats.
Identities = 21/64 (33%), Positives = 28/64 (44%), Gaps = 13/64 (20%)
Query 39 DYRDYESLKNQLSL---QELVFSELG-----YSDEL--FDSFYIKPCIKKLKNSYTDKWK 88
D R+Y KNQL L Q E+G Y+ E+ ++ Y K+ N D W
Sbjct 8 DRRNY---KNQLKLMGQQHQYNKEMGEINQNYAKEMAMINNQYALGMAKESHNMNKDMWN 64
Query 89 DTNY 92
TNY
Sbjct 65 YTNY 68
> Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4
Length=532
Score = 21.9 bits (45), Expect = 1.8, Method: Compositional matrix adjust.
Identities = 24/77 (31%), Positives = 41/77 (53%), Gaps = 6/77 (8%)
Query 37 FYDYRDYESLKNQLSLQELVFSELGYSDELFDSFYIKPCIKKLKNS-YTDKWKDTNYREV 95
FY+ R+ +SL++ E S+ +SD +D K IK+ +S Y + +D + E+
Sbjct 450 FYNERERKSLQDLFHDSEAFESD--WSDIFWDRRQEK--IKRFVDSDYGNLCRDKLHNEI 505
Query 96 HYFRVKHKVLNDENNIF 112
R+KH+ +ND IF
Sbjct 506 RK-RIKHREINDAVGIF 521
> Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4
Length=532
Score = 21.6 bits (44), Expect = 2.5, Method: Compositional matrix adjust.
Identities = 24/77 (31%), Positives = 40/77 (52%), Gaps = 6/77 (8%)
Query 37 FYDYRDYESLKNQLSLQELVFSELGYSDELFDSFYIKPCIKKLKNS-YTDKWKDTNYREV 95
FY+ R+ +SL++ E S+ +SD +D K IK+ +S Y +D + E+
Sbjct 450 FYNERERKSLQDLFHDSEAFESD--WSDIFWDRRQEK--IKRFVDSDYGSLCRDKLHSEI 505
Query 96 HYFRVKHKVLNDENNIF 112
R+KH+ +ND IF
Sbjct 506 RK-RIKHREINDAVGIF 521
Lambda K H a alpha
0.323 0.140 0.424 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 6275005