bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-34_CDS_annotation_glimmer3.pl_2_4
Length=371
Score E
Sequences producing significant alignments: (Bits) Value
Gokush_Human_feces_D_045_Microviridae_AG0399_putative.VP4 161 2e-47
Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4 134 2e-37
Gokush_Human_feces_A_029_Microviridae_AG0222_putative.VP4 121 9e-33
Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4 118 4e-32
Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4 118 4e-32
Gokush_Human_gut_27_035_Microviridae_AG0163_putative.VP4 118 4e-32
Gokush_Human_feces_B_068_Microviridae_AG0328_putative.VP4 114 3e-30
Gokush_Human_feces_A_013_Microviridae_AG011_putative.VP4 103 6e-27
Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4 100 9e-26
Gokush_Human_feces_E_010_Microviridae_AG0123_putative.VP4 98.2 4e-25
> Gokush_Human_feces_D_045_Microviridae_AG0399_putative.VP4
Length=329
Score = 161 bits (407), Expect = 2e-47, Method: Compositional matrix adjust.
Identities = 120/358 (34%), Positives = 170/358 (47%), Gaps = 89/358 (25%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GC++D SR W DRM+ EL DN KA+F+TLTYND L +
Sbjct 59 LGCQIDYSREWRDRMIFELHDNP-KAIFLTLTYNDAHLSFS------------------- 98
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
TL + D Q FMKRLRK F R+RF+LAGEYG + RPHYHAI+
Sbjct 99 -----------DKGCPTLVVSDVQKFMKRLRKHFTGTRIRFYLAGEYGTQNLRPHYHAIL 147
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVY 180
YG+ L+DF D ++ +N+L Q Y S ERIW NG+ +++ V TCAYV+RY MKK
Sbjct 148 YGIDLADFPDLYLRGYNELKQAYYSSPIMERIWKNGFIIMSEVTSRTCAYVARYVMKKQG 207
Query 181 KSENSHAYASGQLPPFCTMSRRPGIGLLHADDLLKKGDKTFIRDIDLNGKECTREVYLGR 240
K+ + H + G LP F SR PG+GLL + D + G+ TF D G++ + + L +
Sbjct 208 KNSDVHV-SRGTLPEFNLSSRNPGLGLLKSRDYVLSGNNTFAFD----GRDGSISISLPK 262
Query 241 AFIRSAAREHMKPVFAAADLVESVQTCINELEAESCTEHEDVLDALYICLRAFRHRLDDV 300
+ +R+ R + + D++D +
Sbjct 263 SMLRNVRR-------------------------NASQKELDIIDRIVY------------ 285
Query 301 IELHIPPSVRLCSRQNERAYNSIQRSISNLAVFGGYLLEYYQGKELHFLQRIKLLPER 358
R+ ++ R SNL V G ++ +GKE +IKLLPER
Sbjct 286 ----------------NRSMDAAARLRSNLDVLGLEFGDFLKGKEKSLTSKIKLLPER 327
> Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4
Length=340
Score = 134 bits (336), Expect = 2e-37, Method: Compositional matrix adjust.
Identities = 98/270 (36%), Positives = 141/270 (52%), Gaps = 28/270 (10%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCRLD SR WA+R +LEL+D+ A FVTLTY+D+ LP + +V + F
Sbjct 67 VGCRLDYSREWANRCMLELEDSS-NAWFVTLTYDDQHLPRSAYVEPETGEAF-------- 117
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
A +L D QLFMKRLR F D ++RFF AGEYG +HRPHYHAI+
Sbjct 118 -------------ASYSLRKTDFQLFMKRLRYYFPDNKIRFFAAGEYGSHSHRPHYHAIL 164
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVY 180
Y + DF D + G + SK + W G+ V+ V W +CAYV+RY MKK
Sbjct 165 YNV---DFDDLEFYKKSLNGDIYWNSKKLDAAWNKGFAVIGEVTWQSCAYVARYCMKKAD 221
Query 181 KSENSHAYASGQLPPFCTMSRRPGIGLLHADDLLKKGDKTFIRDIDLNGKECTREVYLGR 240
+ S+ P F MSR+PGIG ++ D K D + I ++ + +E+ + +
Sbjct 222 GVDASYYEHFNIEPEFTLMSRKPGIGRMYLD---KHPDLYQYQKIFVSTPQGGKEITIPK 278
Query 241 AFIRSAAREHMKPVFAAADLVESVQTCINE 270
F R A+E+ + + A + ++ NE
Sbjct 279 YFDRIVAQENPEMIEALKEKRKAAAIAKNE 308
> Gokush_Human_feces_A_029_Microviridae_AG0222_putative.VP4
Length=343
Score = 121 bits (303), Expect = 9e-33, Method: Compositional matrix adjust.
Identities = 95/228 (42%), Positives = 121/228 (53%), Gaps = 30/228 (13%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCRL+ SR WA+R +LEL+ +D A FVT+TY+D +P + S +
Sbjct 63 IGCRLEYSRQWANRCMLELQYHD-SAYFVTVTYDDEHVPQTYSSDSETGEAL-------- 113
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
TLS RD QLFMKR+RK F D R+R+FLAGEYG T RPHYH I+
Sbjct 114 ------------LPLMTLSKRDMQLFMKRVRKRFCDDRIRYFLAGEYGSTTFRPHYHCIL 161
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGN------GYCVLAPVNWNTCAYVSRY 174
+GL L D K+F G Y S+S W + GY V+APV + TCAYV+RY
Sbjct 162 FGLHLYDLVP-YAKNFR--GDVLYNSQSLSACWCDKSARPMGYVVVAPVTYETCAYVARY 218
Query 175 TMKKVYKSENSHAYASGQLPPFCTMSRRPGIGLLHADDLLKKGDKTFI 222
T KK ++ G PF MSR+PGIG + DD D FI
Sbjct 219 TSKKSGVNDLEAYDLLGLARPFTLMSRKPGIGRQYFDDHPDCMDYDFI 266
> Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4
Length=310
Score = 118 bits (296), Expect = 4e-32, Method: Compositional matrix adjust.
Identities = 81/213 (38%), Positives = 112/213 (53%), Gaps = 24/213 (11%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCR+D SR WA+R LLELK +D A F T TY+D +P ++ +
Sbjct 34 IGCRIDYSRQWANRCLLELKYHD-SAWFCTFTYDDDHVPRTYYPDPETGEAI-------- 84
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
TL RD QL MKR+RK F + ++RFF++GEYG +T RPHYHAI+
Sbjct 85 -------------PALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHAIL 131
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVY 180
+GL L D + + Y S S + W GY V+ V W +CAY +RY MKK+
Sbjct 132 FGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKKL- 190
Query 181 KSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 212
K + + Y + P F MSR+PGI + D+
Sbjct 191 KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDE 223
> Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4
Length=310
Score = 118 bits (296), Expect = 4e-32, Method: Compositional matrix adjust.
Identities = 81/213 (38%), Positives = 112/213 (53%), Gaps = 24/213 (11%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCR+D SR WA+R LLELK +D A F T TY+D +P ++ +
Sbjct 34 IGCRIDYSRQWANRCLLELKYHD-SAWFCTFTYDDDHVPRTYYPDPETGEAI-------- 84
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
TL RD QL MKR+RK F + ++RFF++GEYG +T RPHYHAI+
Sbjct 85 -------------PALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHAIL 131
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVY 180
+GL L D + + Y S S + W GY V+ V W +CAY +RY MKK+
Sbjct 132 FGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKKL- 190
Query 181 KSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 212
K + + Y + P F MSR+PGI + D+
Sbjct 191 KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDE 223
> Gokush_Human_gut_27_035_Microviridae_AG0163_putative.VP4
Length=310
Score = 118 bits (296), Expect = 4e-32, Method: Compositional matrix adjust.
Identities = 81/213 (38%), Positives = 112/213 (53%), Gaps = 24/213 (11%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCR+D SR WA+R LLELK +D A F T TY+D +P ++ +
Sbjct 34 IGCRIDYSRQWANRCLLELKYHD-SAWFCTFTYDDDHVPRTYYPDPETGEAI-------- 84
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
TL RD QL MKR+RK F + ++RFF++GEYG +T RPHYHAI+
Sbjct 85 -------------PALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHAIL 131
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVY 180
+GL L D + + Y S S + W GY V+ V W +CAY +RY MKK+
Sbjct 132 FGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKKL- 190
Query 181 KSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 212
K + + Y + P F MSR+PGI + D+
Sbjct 191 KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDE 223
> Gokush_Human_feces_B_068_Microviridae_AG0328_putative.VP4
Length=351
Score = 114 bits (284), Expect = 3e-30, Method: Compositional matrix adjust.
Identities = 91/222 (41%), Positives = 118/222 (53%), Gaps = 35/222 (16%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCRL+ SR WA+R +LEL + + FVTLTY+D LP +++ +
Sbjct 68 LGCRLEYSRQWANRCMLELGYH-VSSWFVTLTYDDAHLPRSFYGNPD------------- 113
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
A ATL RD QLFMKRLR F D + AGEYG +T RPHYHAII
Sbjct 114 --------TGEAVPCATLYKRDFQLFMKRLRYKFGDGIRFYA-AGEYGDQTKRPHYHAII 164
Query 121 YGLTLSD---FKDCRIKDFNKLGQPRYISKSFERIW----GN--GYCVLAPVNWNTCAYV 171
YGL L D +K ++ N L Y S+S + W GN G+ V+ V W TCAYV
Sbjct 165 YGLELDDLVFYKKMALESAN-LYYNYYNSESLQSCWRDKDGNDIGFVVVGKVTWETCAYV 223
Query 172 SRYTMKKVYKSENSHAYASGQL-PPFCTMSRRPGIGLLHADD 212
+RY MKK K + + Y + P FC MSR+PGI + +D
Sbjct 224 ARYIMKKQ-KGQGADVYERFNIEPEFCLMSRKPGIAHQYYED 264
> Gokush_Human_feces_A_013_Microviridae_AG011_putative.VP4
Length=330
Score = 103 bits (258), Expect = 6e-27, Method: Compositional matrix adjust.
Identities = 76/212 (36%), Positives = 104/212 (49%), Gaps = 28/212 (13%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCR++ SR WA+R++LE + +D A F T TY+D +P +++ +
Sbjct 47 LGCRIEYSRQWANRLMLEREAHD-AAWFCTFTYDDDHVPRSYYPDPETGEAI-------- 97
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
TL RD QL MKR+R+ F D +RFF GEYG +T RPHYHAII
Sbjct 98 -------------PSLTLCKRDFQLLMKRIRRRFPDDHIRFFACGEYGSQTFRPHYHAII 144
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIW----GN--GYCVLAPVNWNTCAYVSRY 174
+GL L D + Y S + W GN G+ V+ V W +CAY +RY
Sbjct 145 FGLHLDDLVPYKTVREGGELYTYYNSPKLQSCWLDSDGNPIGFVVVGEVTWESCAYTARY 204
Query 175 TMKKVYKSENSHAYASGQLPPFCTMSRRPGIG 206
KK+ + E+ P F MSRRPGI
Sbjct 205 VTKKLNRKEHDFYEKHRICPEFSLMSRRPGIA 236
> Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4
Length=305
Score = 100 bits (248), Expect = 9e-26, Method: Compositional matrix adjust.
Identities = 78/209 (37%), Positives = 109/209 (52%), Gaps = 43/209 (21%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCRL +SR WA R ++E K + +F+TLTY+D LP GS +++ F
Sbjct 46 IGCRLSKSREWAARCVVEAKSHK-NNMFLTLTYDDAHLPED---GSLHYEHF-------- 93
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrd---rrlrfflAGEYGPKTHRPHYH 117
QLFMKR+RK F ++LRFF+ GEYG K RPHYH
Sbjct 94 -----------------------QLFMKRMRKYFMSRFGQQLRFFMCGEYGDKLGRPHYH 130
Query 118 AIIYGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMK 177
AII+G+T D + I+ N L Y S++ E++W G+ + VN+ T AYV+RY K
Sbjct 131 AIIFGVTFVDKQLWSIRRGNNL----YRSRTLEKLWPYGFSSIGAVNFETAAYVARYVTK 186
Query 178 KVYKSENSHAYASGQLPPFCTMSRRPGIG 206
K+ Y G++ FC S +PGIG
Sbjct 187 KITGPLKLDHY-DGKVAEFCHCSLKPGIG 214
> Gokush_Human_feces_E_010_Microviridae_AG0123_putative.VP4
Length=305
Score = 98.2 bits (243), Expect = 4e-25, Method: Compositional matrix adjust.
Identities = 67/206 (33%), Positives = 95/206 (46%), Gaps = 37/206 (18%)
Query 1 MGCRLDRSRVWADRMLLELKDNDYKALFVTLTYNDRSLPSAWHVGSNYFDGFYedvvplv 60
+GCRL +SR WA R ++E + + +F+TLTY+D LP + +F F
Sbjct 46 IGCRLSKSREWAARCVVEARSHK-SNMFLTLTYDDAHLPEDHSLHYEHFQLF-------- 96
Query 61 ldddeeWIaaaagapaTLSIRDTQLFMKrlrktfrdrrlrfflAGEYGPKTHRPHYHAII 120
+ T + + FM GEYG K RPHYHAII
Sbjct 97 -------MKRMRKYFQTRFGQQLRFFM----------------CGEYGDKLGRPHYHAII 133
Query 121 YGLTLSDFKDCRIKDFNKLGQPRYISKSFERIWGNGYCVLAPVNWNTCAYVSRYTMKKVY 180
+G+T D + I+ N L Y S + ER+W G+ + VN+ T AYV+RY KK+
Sbjct 134 FGVTFVDKQLWSIRRGNNL----YRSATLERLWPFGFSSIGAVNFETAAYVARYVTKKIT 189
Query 181 KSENSHAYASGQLPPFCTMSRRPGIG 206
Y G++ FC S +PGIG
Sbjct 190 GPLKLEHY-DGKVAEFCHCSLKPGIG 214
Lambda K H a alpha
0.324 0.139 0.438 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 32264491