bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-35_CDS_annotation_glimmer3.pl_2_3
Length=243
Score E
Sequences producing significant alignments: (Bits) Value
Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4 442 3e-159
Gokush_Human_gut_33_003_Microviridae_AG066_putative.VP4 417 8e-149
Gokush_Human_feces_A_019_Microviridae_AG0436_putative.VP4 208 6e-67
Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4 207 1e-66
Gokush_Human_feces_A_020_Microviridae_AG0262_putative.VP4 207 2e-66
Gokush_Human_feces_B_029_Microviridae_AG0416_putative.VP4 182 2e-57
Gokush_Human_feces_C_014_Microviridae_AG0151_putative.VP4 143 5e-42
Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4 115 1e-31
Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4 113 4e-31
Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4 113 4e-31
> Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4
Length=298
Score = 442 bits (1138), Expect = 3e-159, Method: Compositional matrix adjust.
Identities = 215/243 (88%), Positives = 224/243 (92%), Gaps = 0/243 (0%)
Query 1 MILNTGEIMRKVQYVWKPGKKTPESVQTLLYTDVQKFLKRLRKAYKSRLRFFCAGEYGEQ 60
MI+ TGEIMRKVQY WKPGKK PESVQ LLY D+QKFLKRLRKAYK +LR+F AGEYGEQ
Sbjct 56 MIVKTGEIMRKVQYTWKPGKKRPESVQILLYEDIQKFLKRLRKAYKGKLRYFVAGEYGEQ 115
Query 61 TARPHYHMILYGWEPTDLKQLYKIHHNGYYTSEWLEKLWGNGQIQIAQAVPETYRYVAGY 120
TARPHYHMILYGWEPTDLK LYKIHHNGYYTSEWLE LWG GQIQIAQAVPETYRYVAGY
Sbjct 116 TARPHYHMILYGWEPTDLKNLYKIHHNGYYTSEWLENLWGMGQIQIAQAVPETYRYVAGY 175
Query 121 VTKKMYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTNGKQA 180
VTKKMYE+DGKKANAYYELGQ KPFACMSLKPGLGD YYQEHK EIW+QGYIQCTNGKQA
Sbjct 176 VTKKMYELDGKKANAYYELGQTKPFACMSLKPGLGDHYYQEHKAEIWRQGYIQCTNGKQA 235
Query 181 QIPRYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQLETKERVTKKQTKKR 240
QIPRYYEK ME ENP+RLWRIKQNRQ+ AIEQKRLQ E +DYKT LETKERVTKKQTKKR
Sbjct 236 QIPRYYEKQMEAENPQRLWRIKQNRQKNAIEQKRLQLEEQDYKTILETKERVTKKQTKKR 295
Query 241 GIL 243
GIL
Sbjct 296 GIL 298
> Gokush_Human_gut_33_003_Microviridae_AG066_putative.VP4
Length=318
Score = 417 bits (1071), Expect = 8e-149, Method: Compositional matrix adjust.
Identities = 197/243 (81%), Positives = 217/243 (89%), Gaps = 0/243 (0%)
Query 1 MILNTGEIMRKVQYVWKPGKKTPESVQTLLYTDVQKFLKRLRKAYKSRLRFFCAGEYGEQ 60
MI+NTGEIMRKVQY WKPG+K PESVQ LLYTD+QKFLKRLRKAY+ +LR+F AGEYGEQ
Sbjct 76 MIVNTGEIMRKVQYTWKPGEKRPESVQILLYTDIQKFLKRLRKAYRGKLRYFIAGEYGEQ 135
Query 61 TARPHYHMILYGWEPTDLKQLYKIHHNGYYTSEWLEKLWGNGQIQIAQAVPETYRYVAGY 120
TARPHYHMILYGW+PTDL+ LYKI HNGY+TS+WL LWG GQIQIAQAVPETYRYVAGY
Sbjct 136 TARPHYHMILYGWQPTDLEHLYKIQHNGYFTSKWLANLWGMGQIQIAQAVPETYRYVAGY 195
Query 121 VTKKMYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTNGKQA 180
VTKKMYEIDG+KAN YYELGQ KPFACMSLKPGLGD YYQEHK+EIWKQGYIQCTNGK+A
Sbjct 196 VTKKMYEIDGQKANTYYELGQTKPFACMSLKPGLGDHYYQEHKKEIWKQGYIQCTNGKRA 255
Query 181 QIPRYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQLETKERVTKKQTKKR 240
QIPRYYEKMME ENP+RLWRIKQNRQ AI + RL++E D+ Q +TKERV KKQ KK+
Sbjct 256 QIPRYYEKMMEAENPQRLWRIKQNRQAAAIAENRLKYENSDFAEQCKTKERVIKKQMKKK 315
Query 241 GIL 243
G L
Sbjct 316 GTL 318
> Gokush_Human_feces_A_019_Microviridae_AG0436_putative.VP4
Length=338
Score = 208 bits (530), Expect = 6e-67, Method: Compositional matrix adjust.
Identities = 115/242 (48%), Positives = 156/242 (64%), Gaps = 9/242 (4%)
Query 5 TGEIMRKVQYVWKPGKKTPESVQTLLYTDVQKFLKRLRKAYKSRLRFFCAGEYGEQTARP 64
TGE++R Y K K + QTL Y D+QKFLKRLRKA + LR+FCAGEYGE+T RP
Sbjct 103 TGEVIRGALY--KRQKGDLKENQTLWYGDIQKFLKRLRKASEPGLRYFCAGEYGEKTGRP 160
Query 65 HYHMILYGWEPTDLKQLYKIHHNGYYTSEWLEKLWGNGQIQIAQ-AVPETYRYVAGYVTK 123
HYH+IL+ + P L+ + GYYT + K W G +++ A + + Y AGYVTK
Sbjct 161 HYHLILFNYNPEKLEPYRLLSKEGYYTDTRITKCWPYGIHNMSEPATSKAFSYTAGYVTK 220
Query 124 KMYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTNGKQAQIP 183
K+ G + + + G + PFA MSLKPGLG+ YY ++KE IW+QGYIQ +NGK+A IP
Sbjct 221 KI----GDETMEHIKRGLRPPFAMMSLKPGLGEEYYLQNKERIWEQGYIQLSNGKRAAIP 276
Query 184 RYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQ--LETKERVTKKQTKKRG 241
RY+EK ME ENPE+LW IK+ RQ+KA++ + + E D K + L +ER +K K G
Sbjct 277 RYFEKQMEAENPEKLWEIKRQRQQKAMDSTKNKMEQTDIKLEGYLSAQERKIRKFRKSSG 336
Query 242 IL 243
+
Sbjct 337 TM 338
> Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4
Length=338
Score = 207 bits (528), Expect = 1e-66, Method: Compositional matrix adjust.
Identities = 114/242 (47%), Positives = 153/242 (63%), Gaps = 9/242 (4%)
Query 5 TGEIMRKVQYVWKPGKKTPESVQTLLYTDVQKFLKRLRKAYKSRLRFFCAGEYGEQTARP 64
TGE++R Y K K + QTL Y D+QKFLKRLRKA +R+FCAGEYGE T RP
Sbjct 103 TGEVIRGALY--KRQKGDLKENQTLWYEDIQKFLKRLRKAAGPGIRYFCAGEYGEHTGRP 160
Query 65 HYHMILYGWEPTDLKQLYKIHHNGYYTSEWLEKLWGNGQIQIAQ-AVPETYRYVAGYVTK 123
HYH+IL+ + P L+ + GY+T + K W G +++ A + Y Y A YVTK
Sbjct 161 HYHLILFNYNPEKLEPYRLLSKEGYFTDTRITKCWPYGIHNMSEPATSKAYNYTAAYVTK 220
Query 124 KMYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTNGKQAQIP 183
K+ G + + + G + PFA MSLKPGLG+ YY +HKEEIW+QGYIQ +NGK+A IP
Sbjct 221 KI----GDETMEHIKRGLRPPFAMMSLKPGLGEEYYLQHKEEIWEQGYIQLSNGKRAAIP 276
Query 184 RYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQ--LETKERVTKKQTKKRG 241
RY+EK ME E+PERLW IK+ RQ+K+++ + + E D K + L +ER K K G
Sbjct 277 RYFEKQMEAEDPERLWEIKRQRQQKSMDSTKNKMEKTDIKLEGYLSAQERKIHKFRKSSG 336
Query 242 IL 243
+
Sbjct 337 TM 338
> Gokush_Human_feces_A_020_Microviridae_AG0262_putative.VP4
Length=338
Score = 207 bits (526), Expect = 2e-66, Method: Compositional matrix adjust.
Identities = 114/242 (47%), Positives = 155/242 (64%), Gaps = 9/242 (4%)
Query 5 TGEIMRKVQYVWKPGKKTPESVQTLLYTDVQKFLKRLRKAYKSRLRFFCAGEYGEQTARP 64
TGE++R Y K K + QTL Y D+QKFLKRLRKA +R+FCAGEYGE T RP
Sbjct 103 TGEVIRGALY--KRQKGDLKENQTLWYEDIQKFLKRLRKAAGPGIRYFCAGEYGEHTGRP 160
Query 65 HYHMILYGWEPTDLKQLYKIHHNGYYTSEWLEKLWGNGQIQIAQ-AVPETYRYVAGYVTK 123
HYH+IL+ + P L+ + GY+T + K W G +++ A + Y Y AGYVTK
Sbjct 161 HYHLILFNYNPEKLEPYRLLSKEGYFTDTRITKCWPYGIHNMSEPATSKAYSYTAGYVTK 220
Query 124 KMYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTNGKQAQIP 183
K+ G++ + + G + PFA MSLKPGLG+ YY ++KE+IWKQGYIQ +NGK+A IP
Sbjct 221 KI----GEETMEHIKRGLRPPFAMMSLKPGLGEEYYLQNKEQIWKQGYIQLSNGKRAAIP 276
Query 184 RYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQ--LETKERVTKKQTKKRG 241
RY+EK ME E+PERLW IK+ RQ+K+++ + + E D K + L +ER K K G
Sbjct 277 RYFEKQMEAEDPERLWEIKRQRQQKSMDSTKSKMEQTDIKLESYLSAQERKIHKFRKSGG 336
Query 242 IL 243
+
Sbjct 337 TM 338
> Gokush_Human_feces_B_029_Microviridae_AG0416_putative.VP4
Length=292
Score = 182 bits (462), Expect = 2e-57, Method: Compositional matrix adjust.
Identities = 116/252 (46%), Positives = 146/252 (58%), Gaps = 16/252 (6%)
Query 1 MILNTGEIMRKVQYVWKPGKKTPESVQTLLYTDVQKFLKRLRKAYKSR------LRFFCA 54
MI TGE++R + W G+ PE VQ LL D+ F KRLRK ++ LR+F
Sbjct 48 MIRATGEVIRGAVHQWTTGE-VPEVVQILLQEDMVLFNKRLRKKQQTSDKWGLDLRYFYC 106
Query 55 GEYGEQTARPHYHMILYGWEPTDLKQLYKIHHNGYYTSEWLEKLWGNGQIQIAQAVPETY 114
GEYGE T RPH+H I YG E DLK+ K N Y+ SE L K+WG G I IA+A ET
Sbjct 107 GEYGENTGRPHHHGIYYGLEIPDLKK--KRGDNPYFESEELNKIWGMGNIIIAEATAETM 164
Query 115 RYVAGYVTKKMYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQC 174
YVAGYVTKK Y D K+ Y ELG P+ CMS PGLG YY HKE+++K +
Sbjct 165 AYVAGYVTKKAYGNDNKR---YRELGLPTPYCCMSRNPGLGYDYYTSHKEQMYKDDGLY- 220
Query 175 TNGKQAQIPRYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQLETK-ERVT 233
NGK+ IPRY++K EKE+P LW IK+ RQ AI +++ + E E T
Sbjct 221 FNGKKRPIPRYFDKKYEKEDPINLWSIKEKRQSSAINALKIKMSKTGLTIEQEAGIEEET 280
Query 234 KKQT--KKRGIL 243
+KQ K RG+L
Sbjct 281 RKQKFRKARGLL 292
> Gokush_Human_feces_C_014_Microviridae_AG0151_putative.VP4
Length=331
Score = 143 bits (360), Expect = 5e-42, Method: Compositional matrix adjust.
Identities = 82/212 (39%), Positives = 121/212 (57%), Gaps = 17/212 (8%)
Query 6 GEIMRKVQYVWKPGKKTPESVQTLLYTDVQKFLKRLRKAYKSRLRFFCAGEYGEQTARPH 65
GEI++ Q + QT+ DVQ FLKRLRKAYK +++F AGEYG++T RPH
Sbjct 91 GEILKGYQSI---------QSQTVSKRDVQLFLKRLRKAYKKPIKYFIAGEYGDRTKRPH 141
Query 66 YHMILYGWEPTDLKQLYKIHHNGYYTSEWLEKLWGNGQIQIAQAVPETYRYVAGYVTKKM 125
YH I +G +P D N Y+ SEWL +WG G + + A P +Y YVA YV KK
Sbjct 142 YHGIFFGLKPEDGVWYKNQKGNAYFKSEWLTNIWGKGFVDFSPAAPGSYAYVAQYVNKKA 201
Query 126 YEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTNGKQAQIPRY 185
I +++ Y+ G++ F MS G+G+ Y +EH +EI + I C G+Q + PRY
Sbjct 202 --IGAEQSAKYWMQGREPEFRIMS--KGIGEEYLKEHMDEILETDNITCAGGRQKRPPRY 257
Query 186 YEKMMEK---ENPERLWRIKQNRQRKAIEQKR 214
++K++++ E+ E ++ N R A+ KR
Sbjct 258 FDKLLDRDTNEDAESYFKAHSNELR-AVRAKR 288
> Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4
Length=340
Score = 115 bits (287), Expect = 1e-31, Method: Compositional matrix adjust.
Identities = 71/190 (37%), Positives = 104/190 (55%), Gaps = 9/190 (5%)
Query 28 TLLYTDVQKFLKRLRKAY-KSRLRFFCAGEYGEQTARPHYHMILYGWEPTDLKQLYKIHH 86
+L TD Q F+KRLR + +++RFF AGEYG + RPHYH ILY + DL + YK
Sbjct 121 SLRKTDFQLFMKRLRYYFPDNKIRFFAAGEYGSHSHRPHYHAILYNVDFDDL-EFYKKSL 179
Query 87 NG--YYTSEWLEKLWGNGQIQIAQAVPETYRYVAGYVTKKMYEIDGKKANAYYELGQQKP 144
NG Y+ S+ L+ W G I + ++ YVA Y KK DG A+ Y +
Sbjct 180 NGDIYWNSKKLDAAWNKGFAVIGEVTWQSCAYVARYCMKKA---DGVDASYYEHFNIEPE 236
Query 145 FACMSLKPGLGDAYYQEHKEEIWKQGYIQCT--NGKQAQIPRYYEKMMEKENPERLWRIK 202
F MS KPG+G Y +H + Q T GK+ IP+Y+++++ +ENPE + +K
Sbjct 237 FTLMSRKPGIGRMYLDKHPDLYQYQKIFVSTPQGGKEITIPKYFDRIVAQENPEMIEALK 296
Query 203 QNRQRKAIEQ 212
+ R+ AI +
Sbjct 297 EKRKAAAIAK 306
> Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4
Length=310
Score = 113 bits (282), Expect = 4e-31, Method: Compositional matrix adjust.
Identities = 79/229 (34%), Positives = 121/229 (53%), Gaps = 18/229 (8%)
Query 12 VQYVWKPGKKTPESVQ--TLLYTDVQKFLKRLRKAYKS-RLRFFCAGEYGEQTARPHYHM 68
V + P +T E++ TL D Q +KR+RK +++ ++RFF +GEYG QT RPHYH
Sbjct 70 VPRTYYPDPETGEAIPALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHA 129
Query 69 ILYGWEPTDLKQLYKIHHNG----YYTSEWLEKLWGNGQIQIAQAVPETYRYVAGYVTKK 124
IL+G DL+ + G YY S L++ W G + + + E+ Y A YV KK
Sbjct 130 ILFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKK 189
Query 125 MYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTN---GKQAQ 181
+ GK+A Y + Q F+ MS KPG+ Y+ E+ + +Q YI + GK+ +
Sbjct 190 L---KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDENSHCVEEQ-YINVSTPKGGKKFR 245
Query 182 IPRYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQLETKE 230
PRYY+K+ + E PE+ +K R + A + E K T L++ E
Sbjct 246 PPRYYDKLFDIECPEKSAELKSLRAKLAQQ----AMEAKLSNTSLDSYE 290
> Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4
Length=310
Score = 113 bits (282), Expect = 4e-31, Method: Compositional matrix adjust.
Identities = 79/229 (34%), Positives = 121/229 (53%), Gaps = 18/229 (8%)
Query 12 VQYVWKPGKKTPESVQ--TLLYTDVQKFLKRLRKAYKS-RLRFFCAGEYGEQTARPHYHM 68
V + P +T E++ TL D Q +KR+RK +++ ++RFF +GEYG QT RPHYH
Sbjct 70 VPRTYYPDPETGEAIPALTLQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHA 129
Query 69 ILYGWEPTDLKQLYKIHHNG----YYTSEWLEKLWGNGQIQIAQAVPETYRYVAGYVTKK 124
IL+G DL+ + G YY S L++ W G + + + E+ Y A YV KK
Sbjct 130 ILFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKK 189
Query 125 MYEIDGKKANAYYELGQQKPFACMSLKPGLGDAYYQEHKEEIWKQGYIQCTN---GKQAQ 181
+ GK+A Y + Q F+ MS KPG+ Y+ E+ + +Q YI + GK+ +
Sbjct 190 L---KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDENSHCVEEQ-YINVSTPKGGKKFR 245
Query 182 IPRYYEKMMEKENPERLWRIKQNRQRKAIEQKRLQFEGKDYKTQLETKE 230
PRYY+K+ + E PE+ +K R + A + E K T L++ E
Sbjct 246 PPRYYDKLFDIECPEKSAELKSLRAKLAQQ----AMEAKLSNTSLDSYE 290
Lambda K H a alpha
0.318 0.134 0.412 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 19379907