bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-3_CDS_annotation_glimmer3.pl_2_3
Length=515
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4 73.6 1e-15
Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4 70.9 8e-15
Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4 70.9 8e-15
Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4 70.9 8e-15
Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4 70.1 1e-14
Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4 69.7 2e-14
Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4 65.9 1e-13
Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4 60.1 1e-11
Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4 60.1 2e-11
Gokush_Human_feces_A_020_Microviridae_AG0262_putative.VP4 58.9 3e-11
> Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4
Length=532
Score = 73.6 bits (179), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 54/197 (27%), Positives = 89/197 (45%), Gaps = 20/197 (10%)
Query 20 SSIFLYPCRKCECCQVSRQKSLSTMLALEESHAKYCYFINPTYNDFNIPAVRV---PDDA 76
+ + PC C C+ ++ + + +++ YF+ TY IP DA
Sbjct 26 GELIMVPCGTCPACRFNKSILSQNKVHAQSLVSRHVYFVTLTYAQRYIPYYEYEIEALDA 85
Query 77 DFGSIAE-------------FEIITPRLKRDKYFEPYIVDYDVDLEKSIGQLCEQRDEYS 123
DF +I + +L+ P + + + + Q+ S
Sbjct 86 DFLAITAHCCDRNPMYRTYTYRGTKHKLRIRGLASPNVKSFSFSVNRDYWTSYAQKANLS 145
Query 124 RLYSRSHKFVPHDVIYLLHYPDIQRFIKRFRIY-AKRKFNASCRYYVVGEYGTNSLRPHW 182
++ + + + YLLH D+ ++KR R Y +K N + Y+VGEYG +S RPH+
Sbjct 146 --FNGKYPALSGRIPYLLH-GDVSLYMKRVRKYISKLGINETIHTYIVGEYGPSSFRPHF 202
Query 183 HLLLFFDSDELAQDLER 199
HLLLFFDSDELAQ++ R
Sbjct 203 HLLLFFDSDELAQNIIR 219
> Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4
Length=536
Score = 70.9 bits (172), Expect = 8e-15, Method: Compositional matrix adjust.
Identities = 61/212 (29%), Positives = 93/212 (44%), Gaps = 30/212 (14%)
Query 6 PFIKCTSPIAI---HRGSSIFLYPCRKCECCQVSR--QKSLSTMLALEESHAKYCYFINP 60
P+ C P+ I + G I++ C CE C ++ QK L + L S C+F+
Sbjct 8 PYCSCLHPVIIKNKYTGDPIYV-ECGTCEVCLSNKAIQKELRCNIQLASSRC--CFFVTL 64
Query 61 TYNDFNIPAVR------------VPDDADFGSIAEFEIITPRLKRDKYFEPYIVDYDVDL 108
TY +IP R VP D + + + R F DY +L
Sbjct 65 TYATEHIPVARFYKLNDSYHLCCVPRDHVYTYVT-----SQGYNRKMSFCDEEFDYPTNL 119
Query 109 -EKSIGQLCEQRDEYSRLYSRSHKFVPH----DVIYLLHYPDIQRFIKRFRIYAKRKFNA 163
++++ L ++ +Y V + D++ L+Y D+Q F KR K+ +
Sbjct 120 RDEAVTALLDKTHLDRTVYPDGRSVVKYPNMGDLLPYLNYRDVQLFHKRINQQIKKYTDE 179
Query 164 SCRYYVVGEYGTNSLRPHWHLLLFFDSDELAQ 195
Y VGEYG + RPH+HLL FFDS+ LAQ
Sbjct 180 KIYSYTVGEYGPKTFRPHFHLLFFFDSERLAQ 211
> Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4
Length=559
Score = 70.9 bits (172), Expect = 8e-15, Method: Compositional matrix adjust.
Identities = 59/225 (26%), Positives = 97/225 (43%), Gaps = 42/225 (19%)
Query 27 CRKCECCQVSRQKSLSTMLALEESHAKYCYFINPTYNDFNIP-------------AVRVP 73
C +C+ C R + S + S KY YF+ TY++ +IP V +
Sbjct 33 CGQCDYCIHKRAQKASMRVKTAGSAFKYSYFVTLTYDNEHIPLMNCKVLHSEYEDVVGIS 92
Query 74 DDADFGS-------IAEFEIITPRLKRDKYFEPYIVDYDVDLEKSIGQLCEQRDEY---- 122
D FG ++E+ + R +FE V V ++ I + +D +
Sbjct 93 GDIHFGDEYHKYIPVSEYRCDDNSMLRHIFFEQ--VQGTVPFDREIKEYVPVKDNWFLSM 150
Query 123 --------------SRLYSRSHKFVPHDVIYLLHYPDIQRFIKRFRIYAKRKFNA--SCR 166
Y S ++ ++I L+Y D+Q +IKR R Y ++ +
Sbjct 151 AAIRSFIYKTQSVDKTDYPASEQYGRDNLIPFLNYVDVQNYIKRLRKYLFQQLGKYETFH 210
Query 167 YYVVGEYGTNSLRPHWHLLLFFDSDELAQDLERCYHPTDKELRKD 211
+Y VGEYG RPH+HLLLF +SD++++ L C+ + K R D
Sbjct 211 FYAVGEYGPVHFRPHYHLLLFTNSDKVSEVLRYCHDKSWKLGRSD 255
> Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4
Length=546
Score = 70.9 bits (172), Expect = 8e-15, Method: Compositional matrix adjust.
Identities = 49/179 (27%), Positives = 83/179 (46%), Gaps = 13/179 (7%)
Query 27 CRKCECCQVSRQKSLSTMLALEESHAKYCYFINPTYNDFNIPAVRVPDDADFGSIAEFEI 86
C KCE C + S + ++ S +KY F+ TY+ +++P ++ + D + +
Sbjct 34 CGKCEACLSRLASARSIKVGVQASLSKYVMFVTLTYDTYHVPKCKIYSNGDNNYVL---V 90
Query 87 ITPRLKRDKYFEP-------YIVDYDVDLEKSIGQLCEQRDEYSRLYSRSHKFV-PH--D 136
+ PR+K Y+E + YD D + + + + + K PH D
Sbjct 91 VKPRVKDYFYYETSDGQKRLKGLSYDDDFRVEFKADKDYIENFKKQANLDVKGCYPHLKD 150
Query 137 VIYLLHYPDIQRFIKRFRIYAKRKFNASCRYYVVGEYGTNSLRPHWHLLLFFDSDELAQ 195
+ L D Q F+KR R + + Y+VGEY RPH+H+L FF+S+EL+Q
Sbjct 151 MYGYLSRKDCQLFMKRVRKQIRNYTDEKIHTYIVGEYSPKHFRPHFHILFFFNSNELSQ 209
> Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4
Length=532
Score = 70.1 bits (170), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 80/317 (25%), Positives = 126/317 (40%), Gaps = 48/317 (15%)
Query 20 SSIFLYPCRKCECCQVSRQKSLSTMLALEESHAKYCYFINPTYNDFNIPAVRV---PDDA 76
+ + PC C C+ ++ + + +++ YF+ TY IP DA
Sbjct 26 GELIMVPCGTCPACRFNKSILSQNKVHAQSLISRHVYFVTLTYAQRYIPYYEYEIEALDA 85
Query 77 DFGSIAE-------------FEIITPRLKRDKYFEPYIVDYDVDLEKSIGQLCEQRDEYS 123
DF +I + +L+ P + + + + Q+ S
Sbjct 86 DFLAITAHCRDRNPMYRTYTYRGTKHKLRIRGLASPKVKSFSFSVNRDYWTSYAQKANLS 145
Query 124 RLYSRSHKFVPHDVIYLLHYPDIQRFIKRFRIY-AKRKFNASCRYYVVGEYGTNSLRPHW 182
+ + + + YLLH D+ ++KR R Y +K N + YVVGEYG + RPH+
Sbjct 146 --FDGKYPALSDRIPYLLH-DDVSLYMKRVRKYISKLGINETIHTYVVGEYGPVTFRPHF 202
Query 183 HLLLFFDSDELAQDLERCYHPTDKELRKDPDLRFYNDHDCAECLRTLWKFGFATSERTNK 242
HLLLFF+SDELAQ + R R+ W+FG +
Sbjct 203 HLLLFFNSDELAQSIVR-------------------------IARSCWRFGRVDCSASRG 237
Query 243 sayyyvsgyvtstsRFPLCLSALS--RPHSLHSRFFGQTLAEEEIQRAIVSQDFEYFRVH 300
A YVS Y+ S S PL + + RP + S FG + E I++A S DF+
Sbjct 238 DAEDYVSSYLNSFSSIPLHIQEIRAIRPFARFSNKFGFSFFESSIKKA-QSGDFDEILNG 296
Query 301 YRYSSKGYQIPYALWRS 317
G+ WR+
Sbjct 297 KSLPYNGFNTTIFPWRA 313
> Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4
Length=539
Score = 69.7 bits (169), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 58/209 (28%), Positives = 96/209 (46%), Gaps = 23/209 (11%)
Query 10 CTSPIAI---HRGSSIFLYPCRKCECCQVSRQKSLSTMLALEESHAKYCYFINPTYNDFN 66
C P I + G I++ PC CE C ++ ++ + ++YC FI TY+
Sbjct 12 CLHPRVIKNKYTGDPIYV-PCGTCEFCVHNKAIKAELKCNVQLASSRYCEFITLTYSTEY 70
Query 67 IPAVRV----PDDADFGSIAEFEIITPRL----KRDKYFEPYIVDYDVDLEKSIGQLCEQ 118
+P + + F S+ + + + R+ F D+D L S QL ++
Sbjct 71 LPVGKFYQGPRGEVRFCSLPRDFVYSYKTVQGYTRNISFNDKTFDFDTQLSWSSAQLLQK 130
Query 119 RDE--YSR------LYSRSHKFVPHDVIYLLHYPDIQRFIKRFRIYAKRKFNASCRYYVV 170
+ Y+ +Y+R + ++ L+Y DIQ F KR +R N YYVV
Sbjct 131 KAHLHYTSFPDGRCIYNRPYM---EGLVGYLNYHDIQLFFKRLNQNIRRITNEKIYYYVV 187
Query 171 GEYGTNSLRPHWHLLLFFDSDELAQDLER 199
GEYG + RPH+H+LLF DS +L + + +
Sbjct 188 GEYGPTTFRPHFHILLFHDSRKLRESIRQ 216
> Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4
Length=298
Score = 65.9 bits (159), Expect = 1e-13, Method: Compositional matrix adjust.
Identities = 57/216 (26%), Positives = 89/216 (41%), Gaps = 66/216 (31%)
Query 21 SIFLYPCRKCECCQVSRQKSLSTMLALEESH--AKYCYFINPTYNDFNIPAVRVPDDADF 78
+ L PC +C C++ +++ +T + LE + +FI TYND N+P + V
Sbjct 5 KVMLIPCGQCIGCRIRQREDWTTRIELEARDYPKEQVWFITLTYNDDNVPGMIV----KT 60
Query 79 GSIAEFEIITPRLKRDKYFEPYIVDYDVDLEKSIGQLCEQRDEYSRLYSRSHKFVPHDVI 138
G I +++ +Y + K P V
Sbjct 61 GEI---------MRKVQYT-----------------------------WKPGKKRPESVQ 82
Query 139 YLLHYPDIQRFIKRFRIYAKRKFNASCRYYVVGEYGTNSLRPHWHLLLFFDSDELAQDLE 198
LL Y DIQ+F+KR R + + RY+V GEYG + RPH+H++L+
Sbjct 83 ILL-YEDIQKFLKRLR----KAYKGKLRYFVAGEYGEQTARPHYHMILY----------- 126
Query 199 RCYHPTD-KELRKDPDLRFYNDHDCAECLRTLWKFG 233
+ PTD K L K +N + +E L LW G
Sbjct 127 -GWEPTDLKNLYKI----HHNGYYTSEWLENLWGMG 157
> Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4
Length=338
Score = 60.1 bits (144), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 48/169 (28%), Positives = 73/169 (43%), Gaps = 53/169 (31%)
Query 22 IFLYPCRKCECCQVSRQKSLSTMLALEESHAK--YCYFINPTYNDFNIPAVRVPDDADFG 79
+ L PC +C C++ +++ + + +E +K +F+ TY+D NIP + D + G
Sbjct 49 VMLIPCGQCIGCRIRKREDWACRIEMEAKTSKPHSVWFVTLTYDDKNIPGI----DHETG 104
Query 80 SIAEFEIITPRL-KRDKYFEPYIVDYDVDLEKSIGQLCEQRDEYSRLYSRSHKFVPHDVI 138
E+I L KR K G L E +
Sbjct 105 -----EVIRGALYKRQK-----------------GDLKENQ------------------- 123
Query 139 YLLHYPDIQRFIKRFRIYAKRKFNASCRYYVVGEYGTNSLRPHWHLLLF 187
L Y DIQ+F+KR R A RY+ GEYG ++ RPH+HL+LF
Sbjct 124 -TLWYEDIQKFLKRLRKAA----GPGIRYFCAGEYGEHTGRPHYHLILF 167
> Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4
Length=611
Score = 60.1 bits (144), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 63/251 (25%), Positives = 95/251 (38%), Gaps = 61/251 (24%)
Query 7 FIKCTSPIAIHRGSS--IFLYPCRKCECCQVSRQKSLSTMLALEESHAKYCYFINPTYND 64
F +C P I S + PC C+ C +++ + +H KYCYF+ TY D
Sbjct 13 FTECLRPQRIVNPYSHDVIFAPCGHCKSCIMNKSNFATAYAMNMATHFKYCYFVTLTYKD 72
Query 65 -----FNIPAVRVPD-----DADFG---SIAEFEIITPRLKRDKYFEPYIVDYDVDLEKS 111
++ VR D +F S ++ +TP D+ F +V+
Sbjct 73 IFLPYLSVEVVRRSGNRYLFDENFETMVSTSDPRFLTPEYYHDRDFSLDSAQNEVEQVFD 132
Query 112 IG--------------------------------QLCEQRDEYSRLYSRSHKFVPHDVIY 139
+G +L E +D + R + + V+Y
Sbjct 133 VGFQSIPRDVSVKSKGSFRFRSFDDEPLKFCIPMKLTELQDILIKANGR-YDYGKKKVVY 191
Query 140 -----------LLHYPDIQRFIKRFRIYAKRKFNASCR--YYVVGEYGTNSLRPHWHLLL 186
+L DI+ F KR R S + YYVV EYG + RPHWH LL
Sbjct 192 PSLADCKLQIPVLQSRDIELFFKRLRRNLDSHGFTSSKICYYVVSEYGPQTYRPHWHCLL 251
Query 187 FFDSDELAQDL 197
FF+S+E+ Q L
Sbjct 252 FFNSEEITQTL 262
> Gokush_Human_feces_A_020_Microviridae_AG0262_putative.VP4
Length=338
Score = 58.9 bits (141), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 48/169 (28%), Positives = 73/169 (43%), Gaps = 53/169 (31%)
Query 22 IFLYPCRKCECCQVSRQKSLSTMLALEESHAK--YCYFINPTYNDFNIPAVRVPDDADFG 79
+ L PC +C C++ +++ + + +E +K +F+ TY+D NIP V + + G
Sbjct 49 VMLIPCGQCIGCRIRKREDWACRIEMEAKTSKPHSVWFVTLTYDDKNIPGV----NHETG 104
Query 80 SIAEFEIITPRL-KRDKYFEPYIVDYDVDLEKSIGQLCEQRDEYSRLYSRSHKFVPHDVI 138
E+I L KR K G L E +
Sbjct 105 -----EVIRGALYKRQK-----------------GDLKENQ------------------- 123
Query 139 YLLHYPDIQRFIKRFRIYAKRKFNASCRYYVVGEYGTNSLRPHWHLLLF 187
L Y DIQ+F+KR R A RY+ GEYG ++ RPH+HL+LF
Sbjct 124 -TLWYEDIQKFLKRLRKAA----GPGIRYFCAGEYGEHTGRPHYHLILF 167
Lambda K H a alpha
0.326 0.140 0.455 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 46976163