BLASTP 2.2.24 [Aug-08-2010]
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eace_2137_orf2
(156 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
14,777,732 sequences; 5,058,227,080 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|221488780|gb|EEE26994.1| conserved hypothetical protein [Toxo... 46 0.002
gi|237837341|ref|XP_002367968.1| hypothetical protein TGME49_030... 46 0.002
gi|325117770|emb|CBZ53321.1| conserved hypothetical protein [Neo... 44 0.011
gi|145532222|ref|XP_001451872.1| hypothetical protein [Parameciu... 41 0.056
gi|145473743|ref|XP_001462535.1| hypothetical protein [Parameciu... 40 0.099
gi|156088135|ref|XP_001611474.1| transporter, major facilitator ... 39 0.18
gi|145493435|ref|XP_001432713.1| hypothetical protein [Parameciu... 39 0.24
gi|118347505|ref|XP_001007229.1| Major Facilitator Superfamily p... 39 0.26
gi|71663806|ref|XP_818891.1| dispersed gene family protein 1 (DG... 39 0.30
gi|168001733|ref|XP_001753569.1| predicted protein [Physcomitrel... 39 0.34
gi|322825128|gb|EFZ30244.1| dispersed gene family protein 1 (DGF... 37 1.3
gi|71399351|ref|XP_802762.1| dispersed gene family protein 1 (DG... 36 1.8
gi|67621406|ref|XP_667762.1| hypothetical protein [Cryptosporidi... 36 1.8
gi|66363086|ref|XP_628509.1| major facilitator superfamily trans... 36 1.8
gi|322817740|gb|EFZ25375.1| dispersed gene family protein 1 (DGF... 36 1.9
gi|322830662|gb|EFZ33608.1| dispersed gene family protein 1 (DGF... 36 2.1
gi|301094336|ref|XP_002896274.1| Major Facilitator Superfamily (... 36 2.4
gi|338174074|ref|YP_004650884.1| hypothetical protein PUV_00800 ... 35 3.2
gi|282890430|ref|ZP_06298957.1| hypothetical protein pah_c017o01... 35 3.6
gi|145486746|ref|XP_001429379.1| hypothetical protein [Parameciu... 35 3.7
gi|340503314|gb|EGR29914.1| major facilitator superfamily protei... 35 3.7
gi|340505347|gb|EGR31685.1| major facilitator superfamily protei... 34 7.8
gi|209876496|ref|XP_002139690.1| major facilitator superfamily t... 34 8.1
>gi|221488780|gb|EEE26994.1| conserved hypothetical protein [Toxoplasma gondii GT1]
Length = 1162
Score = 46.2 bits (108), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 25/67 (37%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Query: 92 LVGVVCMHLNIYTLLPCVRVCS-----AVIGSFSSRVYVCVACLWLLLCFGAAMLPPLTG 146
L G + + TL C+ + +G+ ++ V LW LLCFG A+LPPLTG
Sbjct: 1050 LGGYKTLDAKLKTLQACLAAAAAAVVCGFVGAVTTDALTFVLSLWFLLCFGGALLPPLTG 1109
Query: 147 IQIDAVS 153
+QI AV
Sbjct: 1110 LQIAAVE 1116
>gi|237837341|ref|XP_002367968.1| hypothetical protein TGME49_030570 [Toxoplasma gondii ME49]
gi|211965632|gb|EEB00828.1| hypothetical protein TGME49_030570 [Toxoplasma gondii ME49]
gi|221509270|gb|EEE34839.1| conserved hypothetical protein [Toxoplasma gondii VEG]
Length = 1154
Score = 46.2 bits (108), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 25/67 (37%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Query: 92 LVGVVCMHLNIYTLLPCVRVCS-----AVIGSFSSRVYVCVACLWLLLCFGAAMLPPLTG 146
L G + + TL C+ + +G+ ++ V LW LLCFG A+LPPLTG
Sbjct: 1042 LGGYKTLDAKLKTLQACLAAAAAAVVCGFVGAVTTDALTFVLSLWFLLCFGGALLPPLTG 1101
Query: 147 IQIDAVS 153
+QI AV
Sbjct: 1102 LQIAAVE 1108
>gi|325117770|emb|CBZ53321.1| conserved hypothetical protein [Neospora caninum Liverpool]
Length = 1153
Score = 43.5 bits (101), Expect = 0.011, Method: Compositional matrix adjust.
Identities = 19/29 (65%), Positives = 22/29 (75%)
Query: 125 VCVACLWLLLCFGAAMLPPLTGIQIDAVS 153
+ V LW LLCFG A+LPPLTG+QI AV
Sbjct: 1052 IFVLALWFLLCFGGALLPPLTGLQIAAVE 1080
>gi|145532222|ref|XP_001451872.1| hypothetical protein [Paramecium tetraurelia strain d4-2]
gi|124419538|emb|CAK84475.1| unnamed protein product [Paramecium tetraurelia]
Length = 489
Score = 41.2 bits (95), Expect = 0.056, Method: Composition-based stats.
Identities = 27/76 (35%), Positives = 36/76 (47%), Gaps = 9/76 (11%)
Query: 90 CSLVGVVCMHLNIY----TLLPCVRVCS-----AVIGSFSSRVYVCVACLWLLLCFGAAM 140
C G++ L Y +L CV CS A F+ + CLWLLL FG A+
Sbjct: 285 CITGGLIAQKLGGYQRAKSLYVCVLYCSLCCISAAPVPFTETFWFGALCLWLLLFFGGAI 344
Query: 141 LPPLTGIQIDAVSPDL 156
+PPL GI + +V L
Sbjct: 345 VPPLMGIMLSSVPKHL 360
>gi|145473743|ref|XP_001462535.1| hypothetical protein [Paramecium tetraurelia strain d4-2]
gi|124430375|emb|CAK95162.1| unnamed protein product [Paramecium tetraurelia]
Length = 489
Score = 40.0 bits (92), Expect = 0.099, Method: Composition-based stats.
Identities = 26/76 (34%), Positives = 35/76 (46%), Gaps = 9/76 (11%)
Query: 90 CSLVGVVCMHLNIY----TLLPCVRVCS-----AVIGSFSSRVYVCVACLWLLLCFGAAM 140
C G++ L Y +L CV CS A F+ + CLW LL FG A+
Sbjct: 285 CITGGLIAQKLGGYQRTKSLYVCVLYCSLCCISAAPVPFTETFWFGALCLWFLLFFGGAI 344
Query: 141 LPPLTGIQIDAVSPDL 156
+PPL GI + +V L
Sbjct: 345 VPPLMGIMLSSVPKHL 360
>gi|156088135|ref|XP_001611474.1| transporter, major facilitator family [Babesia bovis]
gi|154798728|gb|EDO07906.1| transporter, major facilitator family [Babesia bovis]
Length = 655
Score = 39.3 bits (90), Expect = 0.18, Method: Compositional matrix adjust.
Identities = 19/33 (57%), Positives = 21/33 (63%)
Query: 124 YVCVACLWLLLCFGAAMLPPLTGIQIDAVSPDL 156
Y V C+WL+L FG MLPPLT I I VS L
Sbjct: 547 YNLVGCIWLILFFGGGMLPPLTLITISNVSERL 579
>gi|145493435|ref|XP_001432713.1| hypothetical protein [Paramecium tetraurelia strain d4-2]
gi|124399827|emb|CAK65316.1| unnamed protein product [Paramecium tetraurelia]
Length = 485
Score = 38.9 bits (89), Expect = 0.24, Method: Composition-based stats.
Identities = 25/76 (32%), Positives = 35/76 (46%), Gaps = 9/76 (11%)
Query: 90 CSLVGVVCMHLNIY----TLLPCVRVC-----SAVIGSFSSRVYVCVACLWLLLCFGAAM 140
C G++ L Y +L CV C SA F+ + C+W LL FG A+
Sbjct: 285 CITGGLIAQKLGGYERTKSLYICVVYCFICCLSATPVPFTETFWFGALCVWFLLFFGGAI 344
Query: 141 LPPLTGIQIDAVSPDL 156
+PPL GI + +V L
Sbjct: 345 VPPLMGIMLSSVPKHL 360
>gi|118347505|ref|XP_001007229.1| Major Facilitator Superfamily protein [Tetrahymena thermophila]
gi|89288996|gb|EAR86984.1| Major Facilitator Superfamily protein [Tetrahymena thermophila
SB210]
Length = 554
Score = 38.9 bits (89), Expect = 0.26, Method: Composition-based stats.
Identities = 16/38 (42%), Positives = 23/38 (60%)
Query: 119 FSSRVYVCVACLWLLLCFGAAMLPPLTGIQIDAVSPDL 156
F Y + +WLLL FG AM+P LTG+ + A+ +L
Sbjct: 428 FIDAFYASASLVWLLLFFGGAMVPALTGMMLSAIQTEL 465
>gi|71663806|ref|XP_818891.1| dispersed gene family protein 1 (DGF-1) [Trypanosoma cruzi strain CL
Brener]
gi|70884167|gb|EAN97040.1| dispersed gene family protein 1 (DGF-1), putative [Trypanosoma cruzi]
Length = 3483
Score = 38.5 bits (88), Expect = 0.30, Method: Compositional matrix adjust.
Identities = 34/112 (30%), Positives = 45/112 (40%), Gaps = 11/112 (9%)
Query: 34 ASFGYGSWRRLRGRGVNLCVLRCTYRPVVCVEGDFCAALLFVYARVHGKSFWRVSFCSLV 93
ASFG GSW +RG ++ +L P D + L +Y +LV
Sbjct: 2720 ASFGSGSWLSVRGNSISGKILSLPSYP---RSADLVQSTLTLYGNAGSGPVVMDGTVALV 2776
Query: 94 G------VVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLWLLLCFGAA 139
G V C+ LN TL P + +IG F R C C + CF AA
Sbjct: 2777 GAGRRFVVGCLTLNGQTLRPMEYRSAGIIGEF--RPVACGVCDADVRCFAAA 2826
>gi|168001733|ref|XP_001753569.1| predicted protein [Physcomitrella patens subsp. patens]
gi|162695448|gb|EDQ81792.1| predicted protein [Physcomitrella patens subsp. patens]
Length = 399
Score = 38.5 bits (88), Expect = 0.34, Method: Compositional matrix adjust.
Identities = 20/72 (27%), Positives = 37/72 (51%), Gaps = 2/72 (2%)
Query: 63 CVEGDFCAALLFVYARVHGKSFWRVSFCSL-VGVVCMHLNIYTLLPCVRVCSAVIGSFSS 121
C+EGD + F +++ HG WRV + + GVV M + + + +C+++IG +
Sbjct: 220 CIEGDHIINI-FSFSKAHGMMGWRVGYIAYPAGVVGMGAQLLKVQDNIPICASIIGQKLA 278
Query: 122 RVYVCVACLWLL 133
+ V W+L
Sbjct: 279 LAALQVGPEWVL 290
>gi|322825128|gb|EFZ30244.1| dispersed gene family protein 1 (DGF-1), putative [Trypanosoma
cruzi]
Length = 442
Score = 36.6 bits (83), Expect = 1.3, Method: Compositional matrix adjust.
Identities = 33/113 (29%), Positives = 45/113 (39%), Gaps = 11/113 (9%)
Query: 34 ASFGYGSWRRLRGRGVNLCVLRCTYRPVVCVEGDFCAALLFVYARVHGKSFWRVSFCSLV 93
ASF GSW +RG ++ +L P D + L ++ S +LV
Sbjct: 95 ASFVSGSWLSVRGNSISGRLLSVPSYPRTA---DLAQSTLTLHGNAGSGSVVMEGTVALV 151
Query: 94 G------VVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLWLLLCFGAAM 140
G V C+ LN L P + +IG F R C C + CFGAA
Sbjct: 152 GAGRRFVVGCLTLNGQALQPINYRSAGIIGEF--RPVACGVCDADVRCFGAAT 202
>gi|71399351|ref|XP_802762.1| dispersed gene family protein 1 (DGF-1) [Trypanosoma cruzi strain
CL Brener]
gi|70864828|gb|EAN81316.1| dispersed gene family protein 1 (DGF-1), putative [Trypanosoma
cruzi]
Length = 878
Score = 36.2 bits (82), Expect = 1.8, Method: Compositional matrix adjust.
Identities = 32/112 (28%), Positives = 46/112 (41%), Gaps = 11/112 (9%)
Query: 34 ASFGYGSWRRLRGRGVNLCVLRCTYRPVVCVEGDFCAALLFVYARVHGKSFWRVSFCSLV 93
ASF +GSW +RG ++ +L P +F + L ++ S +L
Sbjct: 364 ASFAFGSWLSVRGNSISGRLLSVPSYP---RSAEFAQSTLTLHGNAGSGSVVMDGTVTLG 420
Query: 94 G------VVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLWLLLCFGAA 139
G V C+ LN L P + +IG F R C AC + CF AA
Sbjct: 421 GAGRSFVVGCLTLNGQALQPMDYRSAGIIGKF--RPVACGACDADVHCFAAA 470
>gi|67621406|ref|XP_667762.1| hypothetical protein [Cryptosporidium hominis TU502]
gi|54658917|gb|EAL37524.1| hypothetical protein Chro.70384 [Cryptosporidium hominis]
Length = 635
Score = 36.2 bits (82), Expect = 1.8, Method: Composition-based stats.
Identities = 18/57 (31%), Positives = 32/57 (56%), Gaps = 5/57 (8%)
Query: 103 YTLLPCV--RVCSAVIGSFS---SRVYVCVACLWLLLCFGAAMLPPLTGIQIDAVSP 154
YT+L C+ + + + G+ + V + +W LL FG+ ++PP+TGI + V P
Sbjct: 505 YTMLFCMVSAIFATLFGALALIIDNFTVTIVGVWGLLFFGSFLVPPITGISVGVVEP 561
>gi|66363086|ref|XP_628509.1| major facilitator superfamily transporter [Cryptosporidium parvum
Iowa II]
gi|46229527|gb|EAK90345.1| major facilitator (MFS) superfamily transporter containing 12
transmembrane domains [Cryptosporidium parvum Iowa II]
Length = 635
Score = 36.2 bits (82), Expect = 1.8, Method: Composition-based stats.
Identities = 18/57 (31%), Positives = 32/57 (56%), Gaps = 5/57 (8%)
Query: 103 YTLLPCV--RVCSAVIGSFS---SRVYVCVACLWLLLCFGAAMLPPLTGIQIDAVSP 154
YT+L C+ + + + G+ + V + +W LL FG+ ++PP+TGI + V P
Sbjct: 505 YTMLFCMVSAIFATLFGALALIVDNFTVTIVGVWGLLFFGSFLVPPITGISVGVVEP 561
>gi|322817740|gb|EFZ25375.1| dispersed gene family protein 1 (DGF-1), putative [Trypanosoma
cruzi]
Length = 459
Score = 35.8 bits (81), Expect = 1.9, Method: Compositional matrix adjust.
Identities = 34/112 (30%), Positives = 44/112 (39%), Gaps = 11/112 (9%)
Query: 34 ASFGYGSWRRLRGRGVNLCVLRCTYRPVVCVEGDFCAALLFVYARVHGKSFWRVSFCSLV 93
ASF GSW +RG V+ +L P D + L +Y S +LV
Sbjct: 216 ASFVSGSWLSVRGNSVSGKILSVPSYP---RSADLVQSTLTLYGNAGSGSVVMDGTVALV 272
Query: 94 G------VVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLWLLLCFGAA 139
G V C+ LN L P + +IG F R C C + CF AA
Sbjct: 273 GAGRRFVVGCLTLNGQALQPMDYRSAGIIGEF--RPVACGVCDAGVHCFAAA 322
>gi|322830662|gb|EFZ33608.1| dispersed gene family protein 1 (DGF-1), putative [Trypanosoma
cruzi]
Length = 634
Score = 35.8 bits (81), Expect = 2.1, Method: Compositional matrix adjust.
Identities = 33/112 (29%), Positives = 45/112 (40%), Gaps = 11/112 (9%)
Query: 34 ASFGYGSWRRLRGRGVNLCVLRCTYRPVVCVEGDFCAALLFVYARVHGKSFWRVSFCSLV 93
ASF GSW +RG ++ +L P DF + L +Y S +L
Sbjct: 171 ASFVSGSWLSVRGNSISGRLLSVPSYP---RNADFAQSTLTLYGNAGSGSVVMDGTVALG 227
Query: 94 G------VVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLWLLLCFGAA 139
G V C+ LN L P + + +IG F R C C + CF AA
Sbjct: 228 GAGRKFVVGCLTLNGQALQPMIYRSAGIIGEF--RPVACGVCDADVNCFAAA 277
>gi|301094336|ref|XP_002896274.1| Major Facilitator Superfamily (MFS) [Phytophthora infestans T30-4]
gi|262109669|gb|EEY67721.1| Major Facilitator Superfamily (MFS) [Phytophthora infestans T30-4]
Length = 715
Score = 35.8 bits (81), Expect = 2.4, Method: Compositional matrix adjust.
Identities = 17/42 (40%), Positives = 25/42 (59%)
Query: 114 AVIGSFSSRVYVCVACLWLLLCFGAAMLPPLTGIQIDAVSPD 155
+ + +F + +Y+ LWLLL FG A+LP TGI I V +
Sbjct: 573 SAVTTFFNDIYITAGFLWLLLFFGGAILPACTGIFISVVPAE 614
>gi|338174074|ref|YP_004650884.1| hypothetical protein PUV_00800 [Parachlamydia acanthamoebae UV7]
gi|336478432|emb|CCB85030.1| putative uncharacterized protein [Parachlamydia acanthamoebae UV7]
Length = 490
Score = 35.0 bits (79), Expect = 3.2, Method: Compositional matrix adjust.
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Query: 80 HGKSFWRVSFCSLVGVVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLW 131
H KS W+VS +L G+VC+ +N + L+P + + +F V V V +W
Sbjct: 354 HKKSIWQVSVETLAGLVCVGMN-FALIPSLGKEGGALATFFGFVAVIVLSIW 404
>gi|282890430|ref|ZP_06298957.1| hypothetical protein pah_c017o013 [Parachlamydia acanthamoebae str.
Hall's coccus]
gi|281499684|gb|EFB41976.1| hypothetical protein pah_c017o013 [Parachlamydia acanthamoebae str.
Hall's coccus]
Length = 499
Score = 35.0 bits (79), Expect = 3.6, Method: Compositional matrix adjust.
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Query: 80 HGKSFWRVSFCSLVGVVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLW 131
H KS W+VS +L G+VC+ +N + L+P + + +F V V V +W
Sbjct: 363 HKKSIWQVSVETLAGLVCVGMN-FALIPSLGKEGGALATFFGFVAVIVLSIW 413
>gi|145486746|ref|XP_001429379.1| hypothetical protein [Paramecium tetraurelia strain d4-2]
gi|124396471|emb|CAK61981.1| unnamed protein product [Paramecium tetraurelia]
Length = 462
Score = 35.0 bits (79), Expect = 3.7, Method: Compositional matrix adjust.
Identities = 15/38 (39%), Positives = 22/38 (57%)
Query: 119 FSSRVYVCVACLWLLLCFGAAMLPPLTGIQIDAVSPDL 156
F+ + C+W LL FG A++PPL GI + +V L
Sbjct: 299 FTDTFWFGALCVWFLLFFGGAIVPPLMGIMLSSVPKHL 336
>gi|340503314|gb|EGR29914.1| major facilitator superfamily protein, putative [Ichthyophthirius
multifiliis]
Length = 486
Score = 35.0 bits (79), Expect = 3.7, Method: Composition-based stats.
Identities = 21/70 (30%), Positives = 31/70 (44%), Gaps = 13/70 (18%)
Query: 87 VSFCSLVGVVCMHLNIYTLLPCVRVCSAVIGSFSSRVYVCVACLWLLLCFGAAMLPPLTG 146
+S C L GV C +C+ I F + V LW L+ F AAM+P + G
Sbjct: 305 LSICMLNGV------------CASMCAIPI-PFQKDFSIVVTLLWFLMFFEAAMIPAIMG 351
Query: 147 IQIDAVSPDL 156
+ + +V L
Sbjct: 352 LMLSSVQKKL 361
>gi|340505347|gb|EGR31685.1| major facilitator superfamily protein, putative [Ichthyophthirius
multifiliis]
Length = 365
Score = 33.9 bits (76), Expect = 7.8, Method: Compositional matrix adjust.
Identities = 14/38 (36%), Positives = 22/38 (57%)
Query: 119 FSSRVYVCVACLWLLLCFGAAMLPPLTGIQIDAVSPDL 156
F Y+ + +W LL FG AM+P LTG+ + +V +
Sbjct: 283 FFDSFYLAASSIWCLLFFGGAMVPGLTGMMLSSVEAEF 320
>gi|209876496|ref|XP_002139690.1| major facilitator superfamily transporter [Cryptosporidium muris
RN66]
gi|209555296|gb|EEA05341.1| major facilitator superfamily protein [Cryptosporidium muris RN66]
Length = 652
Score = 33.9 bits (76), Expect = 8.1, Method: Composition-based stats.
Identities = 18/55 (32%), Positives = 31/55 (56%), Gaps = 5/55 (9%)
Query: 103 YTLLPCV--RVCSAVIGSFS---SRVYVCVACLWLLLCFGAAMLPPLTGIQIDAV 152
YT+L C+ + + V G + + +A +W LL FG+ ++PP+TGI + V
Sbjct: 519 YTMLFCLGCSITATVFGIIALVIDHFFATIAGIWGLLFFGSFLVPPITGICVGVV 573
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
Posted date: Jul 22, 2011 4:42 PM
Number of letters in database: 5,058,227,080
Number of sequences in database: 14,777,732
Lambda K H
0.335 0.146 0.519
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14777732
Number of Hits to DB: 1,389,584,683
Number of extensions: 50909562
Number of successful extensions: 155199
Number of sequences better than 10.0: 30
Number of HSP's gapped: 155950
Number of HSP's successfully gapped: 31
Length of query: 156
Length of database: 5,058,227,080
Length adjustment: 118
Effective length of query: 38
Effective length of database: 3,314,454,704
Effective search space: 125949278752
Effective search space used: 125949278752
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 76 (33.9 bits)