bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: eggV2 2,483,276 sequences; 915,453,621 total letters Query= Emax_2055_orf1 Length=85 Score E Sequences producing significant alignments: (Bits) Value 5833.PFL1140w 88.2 6e-17 > 5833.PFL1140w Length=273 Score = 88.2 bits (217), Expect = 6e-17, Method: Composition-based stats. Identities = 45/85 (52%), Positives = 56/85 (65%), Gaps = 8/85 (9%) Query 1 DVDATREAHQHTHQQKAYDELSCSHTESHKKTSSDYLKAIVFGGLDGIVTIFAIVAGCVG 60 DV ++EAH H H E+H D LK I+FG LDGI+TIFAIV+GCVG Sbjct 17 DVVLSKEAHDFYHNLD-------KHGENHN-LDKDNLKTIIFGSLDGIITIFAIVSGCVG 68 Query 61 ANLHPSKVVITGIGNLLADAISMGF 85 A + P++V+I GIGNL A+AISMGF Sbjct 69 AKITPTQVIIIGIGNLFANAISMGF 93 Lambda K H 0.319 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 22515314785 Database: eggV2 Posted date: Dec 15, 2009 4:47 PM Number of letters in database: 915,453,621 Number of sequences in database: 2,483,276 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40