bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_0244_orf2
Length=60
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_111360 cGMP-dependent protein kinase, putative (EC:... 104 7e-23
pfa:PF14_0346 PKG; cGMP-dependent protein kinase (EC:2.7.11.12... 84.0 1e-16
tpv:TP03_0511 cGMP-dependent protein kinase (EC:2.7.1.37); K07... 68.2 6e-12
bbo:BBOV_I004690 19.m02237; cGMP dependent protein kinase (EC:... 65.1 6e-11
cpv:cgd8_750 cyclic nucleotide (cGMP)-dependent protein kinase... 58.2 7e-09
dre:556042 prkar2aa, MGC153742, wu:fb55f12, wu:fj29e12, wu:fj3... 30.8 1.0
dre:378958 rc3, cb952; rabconnectin 3 30.4
mmu:19092 Prkg2, AW212535, CGKII, MGC130517, Prkgr2, cGK-II; p... 29.3 3.0
cel:Y70C5C.6 nhr-112; Nuclear Hormone Receptor family member (... 29.3 3.4
dre:566430 cGMP dependent protein kinase I 29.3 3.6
hsa:4619 MYH1, MGC133384, MYHSA1, MYHa, MyHC-2X/D, MyHC-2x; my... 28.1 6.4
mmu:19085 Prkar1b, AI385716, RIbeta; protein kinase, cAMP depe... 28.1 7.0
xla:444805 prkar1b, MGC82149, prkar1; protein kinase, cAMP-dep... 28.1 7.4
dre:494533 prkar1aa, im:7047729, prkar1a, zgc:92515; protein k... 28.1 7.5
dre:100329979 protein kinase, cAMP-dependent, regulatory, type... 28.1 7.5
dre:550427 prkar1ab, zgc:112145; protein kinase, cAMP-dependen... 28.1 7.7
xla:432155 prkar1a, pkr1; protein kinase, cAMP-dependent, regu... 28.1 7.7
hsa:5593 PRKG2, PRKGR2, cGKII; protein kinase, cGMP-dependent,... 28.1 7.7
hsa:5575 PRKAR1B, PRKAR1; protein kinase, cAMP-dependent, regu... 28.1 7.8
mmu:19084 Prkar1a, 1300018C22Rik, RIalpha, Tse-1, Tse1; protei... 27.7 8.4
hsa:5573 PRKAR1A, CAR, CNC, CNC1, DKFZp779L0468, MGC17251, PKR... 27.7 8.8
> tgo:TGME49_111360 cGMP-dependent protein kinase, putative (EC:2.7.11.12);
K07376 protein kinase, cGMP-dependent [EC:2.7.11.12]
Length=994
Score = 104 bits (259), Expect = 7e-23, Method: Composition-based stats.
Identities = 50/60 (83%), Positives = 55/60 (91%), Gaps = 0/60 (0%)
Query 1 SDQAALWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSFK 60
S + ALWGVQR FRETLKQLSSRNFAENRQFLA V+FF+MLTEAQKNVITNALVV++FK
Sbjct 271 STEGALWGVQRHTFRETLKQLSSRNFAENRQFLASVKFFEMLTEAQKNVITNALVVENFK 330
> pfa:PF14_0346 PKG; cGMP-dependent protein kinase (EC:2.7.11.12);
K07376 protein kinase, cGMP-dependent [EC:2.7.11.12]
Length=853
Score = 84.0 bits (206), Expect = 1e-16, Method: Composition-based stats.
Identities = 39/57 (68%), Positives = 44/57 (77%), Gaps = 0/57 (0%)
Query 4 AALWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSFK 60
LWGVQR FR TLKQLS+RNF ENR F+ V F MLTEAQKN+ITNA V+Q+FK
Sbjct 142 GTLWGVQRSTFRATLKQLSNRNFNENRTFIDSVSVFDMLTEAQKNMITNACVIQNFK 198
> tpv:TP03_0511 cGMP-dependent protein kinase (EC:2.7.1.37); K07376
protein kinase, cGMP-dependent [EC:2.7.11.12]
Length=892
Score = 68.2 bits (165), Expect = 6e-12, Method: Composition-based stats.
Identities = 31/56 (55%), Positives = 40/56 (71%), Gaps = 0/56 (0%)
Query 4 AALWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSF 59
+LWGV R VFRETLK +S +++NR FL V+ F+MLTE QKN++TNA V F
Sbjct 175 GSLWGVNRTVFRETLKNISMEVYSQNRSFLDSVKIFEMLTENQKNMVTNAFVESKF 230
> bbo:BBOV_I004690 19.m02237; cGMP dependent protein kinase (EC:2.7.11.1);
K07376 protein kinase, cGMP-dependent [EC:2.7.11.12]
Length=887
Score = 65.1 bits (157), Expect = 6e-11, Method: Composition-based stats.
Identities = 31/54 (57%), Positives = 39/54 (72%), Gaps = 0/54 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSF 59
LWGV R VFR+TLK++S RN+ ENR+F+ V F+ LTE K+ ITNALV F
Sbjct 171 LWGVTRVVFRDTLKRISLRNYTENREFIDCVTIFENLTEENKSCITNALVELRF 224
> cpv:cgd8_750 cyclic nucleotide (cGMP)-dependent protein kinase
with 3 cNMP binding domains and a Ser/Thr kinase domain ;
K07376 protein kinase, cGMP-dependent [EC:2.7.11.12]
Length=965
Score = 58.2 bits (139), Expect = 7e-09, Method: Composition-based stats.
Identities = 25/58 (43%), Positives = 40/58 (68%), Gaps = 0/58 (0%)
Query 2 DQAALWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSF 59
++ LWG+ R FR+TL+ +SSRN+ ENR F+ + F LT+ QK++++ ALV + F
Sbjct 191 EKGGLWGLGRSTFRDTLRLISSRNYEENRAFIESLSIFSGLTDKQKSLLSEALVREIF 248
> dre:556042 prkar2aa, MGC153742, wu:fb55f12, wu:fj29e12, wu:fj33e06,
zgc:153742; protein kinase, cAMP-dependent, regulatory,
type II, alpha A; K04739 cAMP-dependent protein kinase regulator
Length=397
Score = 30.8 bits (68), Expect = 1.0, Method: Composition-based stats.
Identities = 14/59 (23%), Positives = 31/59 (52%), Gaps = 0/59 (0%)
Query 2 DQAALWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSFK 60
D+ ALWG+ R FR + + +++ F+ + + L +++ I + L V++F+
Sbjct 222 DEGALWGLDRATFRRLIVKNNAKKRRMYECFIESIPLLKSLELSERMKIVDVLGVKTFQ 280
> dre:378958 rc3, cb952; rabconnectin 3
Length=3003
Score = 30.4 bits (67), Expect = 1.4, Method: Composition-based stats.
Identities = 12/28 (42%), Positives = 18/28 (64%), Gaps = 0/28 (0%)
Query 3 QAALWGVQRQVFRETLKQLSSRNFAENR 30
+A LWG+ R + E + Q S NF+E+R
Sbjct 1645 KAVLWGLFRSLHDEKMTQFFSHNFSEDR 1672
> mmu:19092 Prkg2, AW212535, CGKII, MGC130517, Prkgr2, cGK-II;
protein kinase, cGMP-dependent, type II (EC:2.7.11.12); K07376
protein kinase, cGMP-dependent [EC:2.7.11.12]
Length=762
Score = 29.3 bits (64), Expect = 3.0, Method: Composition-based stats.
Identities = 14/53 (26%), Positives = 26/53 (49%), Gaps = 0/53 (0%)
Query 7 WGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSF 59
W + R+VF+ +++ + E R FL V + L E + I + L V+ +
Sbjct 255 WALDREVFQNIMRRTAQARDEEYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYY 307
> cel:Y70C5C.6 nhr-112; Nuclear Hormone Receptor family member
(nhr-112)
Length=342
Score = 29.3 bits (64), Expect = 3.4, Method: Composition-based stats.
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Query 9 VQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQ 57
+Q+ + +E RNF ENR F+A +E Q++ +K + LV +
Sbjct 276 IQKVILKELRNYQIDRNFHENRFFVA-LEALQIIERGEKKFMEEMLVCE 323
> dre:566430 cGMP dependent protein kinase I
Length=528
Score = 29.3 bits (64), Expect = 3.6, Method: Composition-based stats.
Identities = 14/39 (35%), Positives = 19/39 (48%), Gaps = 0/39 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTE 44
LW + RQ F+ + + AE +FL V FQ L E
Sbjct 46 LWAIDRQCFQTIMMRTGLIKHAEYMEFLKSVPTFQGLQE 84
> hsa:4619 MYH1, MGC133384, MYHSA1, MYHa, MyHC-2X/D, MyHC-2x;
myosin, heavy chain 1, skeletal muscle, adult; K10352 myosin
heavy chain
Length=1939
Score = 28.1 bits (61), Expect = 6.4, Method: Compositional matrix adjust.
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 0/40 (0%)
Query 3 QAALWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQML 42
+A L G+ ++ E L QL +R A R FLA VE+ +M+
Sbjct 770 KAGLLGLLEEMRDEKLAQLITRTQAMCRGFLARVEYQKMV 809
> mmu:19085 Prkar1b, AI385716, RIbeta; protein kinase, cAMP dependent
regulatory, type I beta (EC:2.7.11.1); K04739 cAMP-dependent
protein kinase regulator
Length=381
Score = 28.1 bits (61), Expect = 7.0, Method: Composition-based stats.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 223 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADAL 271
> xla:444805 prkar1b, MGC82149, prkar1; protein kinase, cAMP-dependent,
regulatory, type I,beta; K04739 cAMP-dependent protein
kinase regulator
Length=381
Score = 28.1 bits (61), Expect = 7.4, Method: Compositional matrix adjust.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 223 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL 271
> dre:494533 prkar1aa, im:7047729, prkar1a, zgc:92515; protein
kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific
extinguisher 1) a
Length=379
Score = 28.1 bits (61), Expect = 7.5, Method: Compositional matrix adjust.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 221 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL 269
> dre:100329979 protein kinase, cAMP-dependent, regulatory, type
I, alpha (tissue specific extinguisher 1) a-like; K04739
cAMP-dependent protein kinase regulator
Length=379
Score = 28.1 bits (61), Expect = 7.5, Method: Compositional matrix adjust.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 221 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL 269
> dre:550427 prkar1ab, zgc:112145; protein kinase, cAMP-dependent,
regulatory, type I, alpha (tissue specific extinguisher
1) b (EC:2.7.11.1); K04739 cAMP-dependent protein kinase regulator
Length=379
Score = 28.1 bits (61), Expect = 7.7, Method: Compositional matrix adjust.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 221 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL 269
> xla:432155 prkar1a, pkr1; protein kinase, cAMP-dependent, regulatory,
type I, alpha (tissue specific extinguisher 1) (EC:2.7.11.1);
K04739 cAMP-dependent protein kinase regulator
Length=381
Score = 28.1 bits (61), Expect = 7.7, Method: Compositional matrix adjust.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 223 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL 271
> hsa:5593 PRKG2, PRKGR2, cGKII; protein kinase, cGMP-dependent,
type II (EC:2.7.11.12); K07376 protein kinase, cGMP-dependent
[EC:2.7.11.12]
Length=762
Score = 28.1 bits (61), Expect = 7.7, Method: Composition-based stats.
Identities = 13/53 (24%), Positives = 26/53 (49%), Gaps = 0/53 (0%)
Query 7 WGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNALVVQSF 59
W + R+VF+ +++ + + R FL V + L E + I + L V+ +
Sbjct 255 WALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYY 307
> hsa:5575 PRKAR1B, PRKAR1; protein kinase, cAMP-dependent, regulatory,
type I, beta (EC:2.7.11.1); K04739 cAMP-dependent
protein kinase regulator
Length=381
Score = 28.1 bits (61), Expect = 7.8, Method: Composition-based stats.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 223 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADAL 271
> mmu:19084 Prkar1a, 1300018C22Rik, RIalpha, Tse-1, Tse1; protein
kinase, cAMP dependent regulatory, type I, alpha (EC:2.7.11.1);
K04739 cAMP-dependent protein kinase regulator
Length=381
Score = 27.7 bits (60), Expect = 8.4, Method: Compositional matrix adjust.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 223 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL 271
> hsa:5573 PRKAR1A, CAR, CNC, CNC1, DKFZp779L0468, MGC17251, PKR1,
PPNAD1, PRKAR1, TSE1; protein kinase, cAMP-dependent, regulatory,
type I, alpha (tissue specific extinguisher 1) (EC:2.7.11.1);
K04739 cAMP-dependent protein kinase regulator
Length=381
Score = 27.7 bits (60), Expect = 8.8, Method: Compositional matrix adjust.
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 0/49 (0%)
Query 6 LWGVQRQVFRETLKQLSSRNFAENRQFLAPVEFFQMLTEAQKNVITNAL 54
LWG+ R +R L + R +FL+ V + L + ++ + +AL
Sbjct 223 LWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL 271
Lambda K H
0.322 0.129 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2069361540
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40