bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_1442_orf1
Length=76
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_054140 transcription activator, putative ; K03009 D... 107 1e-23
pfa:MAL13P1.213 transcription activator, putative; K03009 DNA-... 77.0 1e-14
cpv:cgd7_3240 transcription activator ; K03009 DNA-directed RN... 76.3 2e-14
tpv:TP03_0016 transcription activator; K03009 DNA-directed RNA... 74.3 8e-14
ath:AT5G41010 NRPB12; NRPB12; DNA binding / DNA-directed RNA p... 65.1 5e-11
cel:F23B2.13 rpb-12; RNA Polymerase II (B) subunit family memb... 58.9 4e-09
sce:YHR143W-A RPC10, RPB12; ABC10-alpha; K03009 DNA-directed R... 54.7 7e-08
mmu:17749 Polr2k, MafY, Mt1a, RPABC4, RPB10alpha, RPB12, RPB7.... 53.5 2e-07
dre:796803 MGC171795; zgc:171795; K03009 DNA-directed RNA poly... 52.4 3e-07
hsa:5440 POLR2K, ABC10-alpha, RPABC4, RPB10alpha, RPB12, RPB7.... 52.4 3e-07
mmu:100046796 DNA-directed RNA polymerases I, II, and III subu... 52.4 3e-07
xla:447189 polr2k-b, abc10-alpha, polr2kb, rpabc4, rpb10alpha,... 52.0 4e-07
xla:443871 polr2k-a, abc10-alpha, polr2ka, rpabc4, rpb10alpha,... 52.0 4e-07
mmu:100502981 DNA-directed RNA polymerases I, II, and III subu... 52.0 5e-07
mmu:100503231 DNA-directed RNA polymerases I, II, and III subu... 51.2 7e-07
mmu:100504598 DNA-directed RNA polymerases I, II, and III subu... 48.9 4e-06
ath:AT1G53690 DNA-directed RNA polymerases I, II, and III 7 kD... 43.5 2e-04
mmu:232337 Zfp637, AI646709, BC021601, MGC54806, Znf32; zinc f... 30.8 1.1
hsa:7580 ZNF32, KOX30; zinc finger protein 32 30.4
hsa:9940 DLEC1, DLC1, F56; deleted in lung and esophageal canc... 30.0 2.1
> tgo:TGME49_054140 transcription activator, putative ; K03009
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Length=104
Score = 107 bits (267), Expect = 1e-23, Method: Compositional matrix adjust.
Identities = 50/63 (79%), Positives = 55/63 (87%), Gaps = 3/63 (4%)
Query 14 RPQPAAKMDIEDNNAQLEPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQY 73
RPQ + ++D N+Q+EPVTYICGECGCDV LQP AAVRCRSCGSRILYKKRSRKMMQY
Sbjct 45 RPQGSG---LDDGNSQVEPVTYICGECGCDVMLQPNAAVRCRSCGSRILYKKRSRKMMQY 101
Query 74 EAR 76
EAR
Sbjct 102 EAR 104
> pfa:MAL13P1.213 transcription activator, putative; K03009 DNA-directed
RNA polymerases I, II, and III subunit RPABC4
Length=58
Score = 77.0 bits (188), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 31/53 (58%), Positives = 42/53 (79%), Gaps = 0/53 (0%)
Query 24 EDNNAQLEPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+D + EPV YICGECG D + P A++RC++CGS+I +KKRSR++MQYEAR
Sbjct 6 QDEDISTEPVVYICGECGIDTVIPPNASLRCKNCGSKIFFKKRSRRVMQYEAR 58
> cpv:cgd7_3240 transcription activator ; K03009 DNA-directed
RNA polymerases I, II, and III subunit RPABC4
Length=49
Score = 76.3 bits (186), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 37/48 (77%), Positives = 42/48 (87%), Gaps = 1/48 (2%)
Query 30 LEPVTYICGECGCDVTL-QPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+EPVTYICGECG DV+L AAVRCR+CG RILYKKR+RKM+QYEAR
Sbjct 2 VEPVTYICGECGADVSLLSHGAAVRCRTCGCRILYKKRTRKMIQYEAR 49
> tpv:TP03_0016 transcription activator; K03009 DNA-directed RNA
polymerases I, II, and III subunit RPABC4
Length=58
Score = 74.3 bits (181), Expect = 8e-14, Method: Compositional matrix adjust.
Identities = 32/47 (68%), Positives = 39/47 (82%), Gaps = 0/47 (0%)
Query 23 IEDNNAQLEPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRK 69
DN+ EP+TYICGECG DV+LQP++ VRCR+CGSRILYKKRS +
Sbjct 5 FHDNDDLTEPITYICGECGHDVSLQPSSVVRCRNCGSRILYKKRSYR 51
> ath:AT5G41010 NRPB12; NRPB12; DNA binding / DNA-directed RNA
polymerase; K03009 DNA-directed RNA polymerases I, II, and
III subunit RPABC4
Length=51
Score = 65.1 bits (157), Expect = 5e-11, Method: Compositional matrix adjust.
Identities = 28/46 (60%), Positives = 38/46 (82%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
EPVTY+CG+CG + TL+ ++CR CG RILYKKR+R+++QYEAR
Sbjct 6 EPVTYVCGDCGQENTLKSGDVIQCRECGYRILYKKRTRRVVQYEAR 51
> cel:F23B2.13 rpb-12; RNA Polymerase II (B) subunit family member
(rpb-12); K03009 DNA-directed RNA polymerases I, II, and
III subunit RPABC4
Length=62
Score = 58.9 bits (141), Expect = 4e-09, Method: Compositional matrix adjust.
Identities = 26/44 (59%), Positives = 33/44 (75%), Gaps = 0/44 (0%)
Query 33 VTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+ YICGEC + ++P A+RCR CG RILYKKR RK+M Y+AR
Sbjct 19 MIYICGECHAENEIKPKDAIRCRECGYRILYKKRCRKLMVYDAR 62
> sce:YHR143W-A RPC10, RPB12; ABC10-alpha; K03009 DNA-directed
RNA polymerases I, II, and III subunit RPABC4
Length=70
Score = 54.7 bits (130), Expect = 7e-08, Method: Compositional matrix adjust.
Identities = 23/51 (45%), Positives = 35/51 (68%), Gaps = 0/51 (0%)
Query 26 NNAQLEPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+ A+ + YIC EC ++L T AVRC+ CG RIL K R+++++Q+EAR
Sbjct 20 SQARTATLKYICAECSSKLSLSRTDAVRCKDCGHRILLKARTKRLVQFEAR 70
> mmu:17749 Polr2k, MafY, Mt1a, RPABC4, RPB10alpha, RPB12, RPB7.0;
polymerase (RNA) II (DNA directed) polypeptide K (EC:2.7.7.6)
Length=99
Score = 53.5 bits (127), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 20/46 (43%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ +RCR CG RI+YKKR+++++ ++AR
Sbjct 54 QPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR 99
> dre:796803 MGC171795; zgc:171795; K03009 DNA-directed RNA polymerases
I, II, and III subunit RPABC4
Length=58
Score = 52.4 bits (124), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 20/46 (43%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ +RCR CG RI+YKKR+++++ ++AR
Sbjct 13 QPMIYICGECHTENEIKARDPIRCRECGYRIMYKKRTKRLVVFDAR 58
> hsa:5440 POLR2K, ABC10-alpha, RPABC4, RPB10alpha, RPB12, RPB7.0,
hRPB7.0, hsRPB10a; polymerase (RNA) II (DNA directed) polypeptide
K, 7.0kDa (EC:2.7.7.6); K03009 DNA-directed RNA polymerases
I, II, and III subunit RPABC4
Length=58
Score = 52.4 bits (124), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 20/46 (43%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ +RCR CG RI+YKKR+++++ ++AR
Sbjct 13 QPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR 58
> mmu:100046796 DNA-directed RNA polymerases I, II, and III subunit
RPABC4-like; K03009 DNA-directed RNA polymerases I, II,
and III subunit RPABC4
Length=58
Score = 52.4 bits (124), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 20/46 (43%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ +RCR CG RI+YKKR+++++ ++AR
Sbjct 13 QPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR 58
> xla:447189 polr2k-b, abc10-alpha, polr2kb, rpabc4, rpb10alpha,
rpb12, rpb7.0; polymerase (RNA) II (DNA directed) polypeptide
K, 7.0kDa
Length=58
Score = 52.0 bits (123), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 20/46 (43%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ +RCR CG RI+YKKR+++++ ++AR
Sbjct 13 QPMIYICGECHTENEIKARDPIRCRECGYRIMYKKRTKRLVVFDAR 58
> xla:443871 polr2k-a, abc10-alpha, polr2ka, rpabc4, rpb10alpha,
rpb12, rpb7.0; polymerase (RNA) II (DNA directed) polypeptide
K, 7.0kDa; K03009 DNA-directed RNA polymerases I, II, and
III subunit RPABC4
Length=58
Score = 52.0 bits (123), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 20/46 (43%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ +RCR CG RI+YKKR+++++ ++AR
Sbjct 13 QPMIYICGECHTENEIKARDPIRCRECGYRIMYKKRTKRLVVFDAR 58
> mmu:100502981 DNA-directed RNA polymerases I, II, and III subunit
RPABC4-like
Length=58
Score = 52.0 bits (123), Expect = 5e-07, Method: Compositional matrix adjust.
Identities = 20/46 (43%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ ++RCR CG RI+YKKR+++++ + AR
Sbjct 13 QPMIYICGECHTENEIKSRDSIRCRECGYRIMYKKRTKRLVVFYAR 58
> mmu:100503231 DNA-directed RNA polymerases I, II, and III subunit
RPABC4-like
Length=58
Score = 51.2 bits (121), Expect = 7e-07, Method: Compositional matrix adjust.
Identities = 19/46 (41%), Positives = 34/46 (73%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ YICGEC + ++ +RCR CG RI+Y+KR+++++ ++AR
Sbjct 13 QPMIYICGECHTENEIKSRDPIRCRECGYRIMYRKRTKRLVVFDAR 58
> mmu:100504598 DNA-directed RNA polymerases I, II, and III subunit
RPABC4-like
Length=58
Score = 48.9 bits (115), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 19/46 (41%), Positives = 33/46 (71%), Gaps = 0/46 (0%)
Query 31 EPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKRSRKMMQYEAR 76
+P+ ICGEC + ++ +RCR CG RI+YKKR+++++ ++AR
Sbjct 13 QPMIDICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR 58
> ath:AT1G53690 DNA-directed RNA polymerases I, II, and III 7
kDa subunit, putative
Length=61
Score = 43.5 bits (101), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 19/42 (45%), Positives = 26/42 (61%), Gaps = 0/42 (0%)
Query 25 DNNAQLEPVTYICGECGCDVTLQPTAAVRCRSCGSRILYKKR 66
D+ + V Y+CG+CG + L+ +CR CG RILYKKR
Sbjct 9 DDKQPEQLVIYVCGDCGQENILKRGDVFQCRDCGFRILYKKR 50
> mmu:232337 Zfp637, AI646709, BC021601, MGC54806, Znf32; zinc
finger protein 637
Length=272
Score = 30.8 bits (68), Expect = 1.1, Method: Compositional matrix adjust.
Identities = 18/56 (32%), Positives = 24/56 (42%), Gaps = 5/56 (8%)
Query 6 HKRTSIHTRPQPAAKMDIEDNNAQLEPVTYICGECGCDVTLQPTAAVRCRSCGSRI 61
H R S HTR + Y+CG+CG T + + AV RSC R+
Sbjct 220 HCRKSFHTRGNCLLHGKVHTGE-----TPYLCGQCGKSFTQRGSLAVHQRSCSQRL 270
> hsa:7580 ZNF32, KOX30; zinc finger protein 32
Length=273
Score = 30.4 bits (67), Expect = 1.5, Method: Compositional matrix adjust.
Identities = 18/54 (33%), Positives = 23/54 (42%), Gaps = 5/54 (9%)
Query 8 RTSIHTRPQPAAKMDIEDNNAQLEPVTYICGECGCDVTLQPTAAVRCRSCGSRI 61
R S HTR I Y+CG+CG T + + AV RSC R+
Sbjct 223 RKSFHTRGNCILHGKIHTGE-----TPYLCGQCGKSFTQRGSLAVHQRSCSQRL 271
> hsa:9940 DLEC1, DLC1, F56; deleted in lung and esophageal cancer
1
Length=1755
Score = 30.0 bits (66), Expect = 2.1, Method: Composition-based stats.
Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 7/46 (15%)
Query 10 SIHTRPQPAAKMDIEDNNAQLEPVTYICGECGCDVTLQPTAAVRCR 55
S+ RP+ + DIE ++ QL + GEC D+TL+ A+ C+
Sbjct 901 SLQERPEDVSPFDIEPSSGQLHSL----GECRVDITLE---ALHCQ 939
Lambda K H
0.322 0.133 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2076916240
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40