bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_1861_orf1
Length=126
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_088680 endonuclease V, putative (EC:3.1.21.2); K107... 184 6e-47
tpv:TP02_0334 apurinic/apyrimidinic endonuclease; K10771 AP en... 181 7e-46
bbo:BBOV_III005900 17.m07523; apurinic endonuclease (APN1) fam... 175 3e-44
pfa:PF13_0176 apn1; apurinic/apyrimidinic endonuclease Apn1 (E... 170 1e-42
cpv:cgd1_310 AP endonuclease of the TIM barrel fold, possible ... 162 3e-40
sce:YKL114C APN1; Apn1p (EC:4.2.99.18); K10771 AP endonuclease... 145 3e-35
dre:558198 APurinic/apyrimidinic endoNuclease family member (a... 141 5e-34
eco:b2159 nfo, ECK2152, JW2146; endonuclease IV with intrinsic... 137 8e-33
cel:T05H10.2 apn-1; APurinic/apyrimidinic endoNuclease family ... 135 4e-32
ath:AT4G20060 EMB1895 (EMBRYO DEFECTIVE 1895); binding; K13144... 29.6 2.4
mmu:16179 Irak1, AA408924, IRAK, IRAK-1, IRAK1-S, Il1rak, Plpk... 29.6 2.8
eco:b4003 zraS, ECK3995, hydH, JW3967; sensory histidine kinas... 29.3 3.5
pfa:PF14_0613 conserved Plasmodium membrane protein, unknown f... 28.9 3.8
ath:AT3G10320 transferase, transferring glycosyl groups 28.9 4.5
> tgo:TGME49_088680 endonuclease V, putative (EC:3.1.21.2); K10771
AP endonuclease 1 [EC:4.2.99.18]
Length=667
Score = 184 bits (467), Expect = 6e-47, Method: Composition-based stats.
Identities = 86/126 (68%), Positives = 105/126 (83%), Gaps = 0/126 (0%)
Query 1 SKEEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVC 60
+KEE IR+IA +N+A TK V ++LENMAGQKNV+ S+FEDLRDII LV+ KDR+GVC
Sbjct 498 TKEESIRHIAECLNKAIAATKSVTILLENMAGQKNVLCSEFEDLRDIIALVDRKDRIGVC 557
Query 61 LDTCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKG 120
LDTCHL++AG+DI+T EKFE VM+ FDSIVG K+LK MH+NDSK+ L SGLDRHE LGKG
Sbjct 558 LDTCHLYSAGHDIKTEEKFEAVMKKFDSIVGMKYLKAMHINDSKAPLGSGLDRHEHLGKG 617
Query 121 HLGLEP 126
LG+ P
Sbjct 618 TLGMAP 623
> tpv:TP02_0334 apurinic/apyrimidinic endonuclease; K10771 AP
endonuclease 1 [EC:4.2.99.18]
Length=422
Score = 181 bits (458), Expect = 7e-46, Method: Composition-based stats.
Identities = 84/121 (69%), Positives = 103/121 (85%), Gaps = 0/121 (0%)
Query 2 KEEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVCL 61
K EGI++I+ +N+A + TK V +VLEN AGQKNVIGSKFEDLR IID VEDK RVG+CL
Sbjct 257 KSEGIQHISDCINKALEMTKGVTIVLENAAGQKNVIGSKFEDLRQIIDKVEDKSRVGICL 316
Query 62 DTCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKGH 121
DTCHLFAAGYDIRT E+F++VM++FD +VG K+L+ +HLNDSKS+L SGLDRHE +GKG
Sbjct 317 DTCHLFAAGYDIRTTEQFDEVMKEFDKVVGLKYLRAVHLNDSKSDLGSGLDRHENIGKGK 376
Query 122 L 122
L
Sbjct 377 L 377
> bbo:BBOV_III005900 17.m07523; apurinic endonuclease (APN1) family
protein; K10771 AP endonuclease 1 [EC:4.2.99.18]
Length=374
Score = 175 bits (444), Expect = 3e-44, Method: Compositional matrix adjust.
Identities = 86/124 (69%), Positives = 97/124 (78%), Gaps = 0/124 (0%)
Query 2 KEEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVCL 61
K EGIRNIA +N A K+T +VLEN AGQKNVIGS FEDLRDII+LVE+KDRV VCL
Sbjct 201 KPEGIRNIANCINMAMKETSSAKIVLENAAGQKNVIGSTFEDLRDIINLVENKDRVAVCL 260
Query 62 DTCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKGH 121
DTCHLFAAGYDIRT +KFE VM FD I+G K+L +HLND KS+L SGLDRHE +G G
Sbjct 261 DTCHLFAAGYDIRTKDKFEAVMRSFDEIIGLKYLVAVHLNDCKSDLGSGLDRHENIGIGK 320
Query 122 LGLE 125
L E
Sbjct 321 LTRE 324
> pfa:PF13_0176 apn1; apurinic/apyrimidinic endonuclease Apn1
(EC:4.2.99.18); K10771 AP endonuclease 1 [EC:4.2.99.18]
Length=597
Score = 170 bits (430), Expect = 1e-42, Method: Composition-based stats.
Identities = 79/123 (64%), Positives = 101/123 (82%), Gaps = 0/123 (0%)
Query 3 EEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVCLD 62
+EGI+N+A +N+ HK+T V++VLEN AGQKN +GSKFE LRDII+LV DKDR+GVCLD
Sbjct 431 DEGIKNVADCINKVHKETNNVIIVLENSAGQKNSVGSKFEHLRDIINLVHDKDRIGVCLD 490
Query 63 TCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKGHL 122
TCH FAAGY+I+T E F+ VM+ FD IV K+LK +HLNDSKS++ SGLDRHE +GKG L
Sbjct 491 TCHTFAAGYNIKTFENFDNVMKQFDDIVNVKYLKAVHLNDSKSDIGSGLDRHENIGKGKL 550
Query 123 GLE 125
++
Sbjct 551 TMD 553
> cpv:cgd1_310 AP endonuclease of the TIM barrel fold, possible
bacterial horizontal transfer ; K10771 AP endonuclease 1 [EC:4.2.99.18]
Length=364
Score = 162 bits (410), Expect = 3e-40, Method: Compositional matrix adjust.
Identities = 75/125 (60%), Positives = 101/125 (80%), Gaps = 1/125 (0%)
Query 1 SKEEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVC 60
SKEE I+ I+ +N+AH+ TK V+ VLEN A K +G +F++L +II+ V+DK R+GVC
Sbjct 196 SKEESIQFISDCINKAHEQTKSVITVLENCAESK-CVGYRFKELSEIIERVKDKSRIGVC 254
Query 61 LDTCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKG 120
LDTCHLFAAGYD+RTAE F KVM +F++++G K+LK MHLNDSK + +SGLDRHE LGKG
Sbjct 255 LDTCHLFAAGYDVRTAESFNKVMVEFEAVIGLKYLKAMHLNDSKGQFNSGLDRHENLGKG 314
Query 121 HLGLE 125
++G+E
Sbjct 315 NIGME 319
> sce:YKL114C APN1; Apn1p (EC:4.2.99.18); K10771 AP endonuclease
1 [EC:4.2.99.18]
Length=367
Score = 145 bits (367), Expect = 3e-35, Method: Compositional matrix adjust.
Identities = 61/120 (50%), Positives = 92/120 (76%), Gaps = 0/120 (0%)
Query 6 IRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVCLDTCH 65
++ +A+ +N+A K+TKFV +VLENMAG N++GS DL+++I ++EDK R+GVC+DTCH
Sbjct 136 LKQLASYLNKAIKETKFVKIVLENMAGTGNLVGSSLVDLKEVIGMIEDKSRIGVCIDTCH 195
Query 66 LFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKGHLGLE 125
FAAGYDI T E F ++F+ ++G+K+L +HLNDSK+ L + D HE LG+G+LG++
Sbjct 196 TFAAGYDISTTETFNNFWKEFNDVIGFKYLSAVHLNDSKAPLGANRDLHERLGQGYLGID 255
> dre:558198 APurinic/apyrimidinic endoNuclease family member
(apn-1)-like
Length=296
Score = 141 bits (356), Expect = 5e-34, Method: Compositional matrix adjust.
Identities = 65/124 (52%), Positives = 87/124 (70%), Gaps = 0/124 (0%)
Query 1 SKEEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVC 60
S E+ + IA A+N AH+ T V+ VLENM+GQ + IG +F +L+ IID V DK RVGVC
Sbjct 127 SPEKCMEKIAQAINHAHQQTPAVITVLENMSGQGSTIGGQFSELKGIIDRVRDKSRVGVC 186
Query 61 LDTCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKG 120
LDTCH FAAGYDI ++++FD +VG +L+ +HLNDSK +L S LDRHE +G G
Sbjct 187 LDTCHAFAAGYDISPPGGVNNMLDEFDRVVGLHYLRAVHLNDSKGKLGSHLDRHEDIGHG 246
Query 121 HLGL 124
+G+
Sbjct 247 QIGI 250
> eco:b2159 nfo, ECK2152, JW2146; endonuclease IV with intrinsic
3'-5' exonuclease activity (EC:3.1.21.2); K01151 deoxyribonuclease
IV [EC:3.1.21.2]
Length=285
Score = 137 bits (345), Expect = 8e-33, Method: Compositional matrix adjust.
Identities = 67/123 (54%), Positives = 87/123 (70%), Gaps = 0/123 (0%)
Query 1 SKEEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVC 60
S+E+ + IA ++N A T+ V V+EN AGQ + +G KFE L IID VEDK RVGVC
Sbjct 118 SEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEHLAAIIDGVEDKSRVGVC 177
Query 61 LDTCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKG 120
+DTCH FAAGYD+RT + EK DF VG+K+L+GMHLND+KS S +DRH LG+G
Sbjct 178 IDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLNDAKSTFGSRVDRHHSLGEG 237
Query 121 HLG 123
++G
Sbjct 238 NIG 240
> cel:T05H10.2 apn-1; APurinic/apyrimidinic endoNuclease family
member (apn-1); K10771 AP endonuclease 1 [EC:4.2.99.18]
Length=396
Score = 135 bits (339), Expect = 4e-32, Method: Composition-based stats.
Identities = 63/122 (51%), Positives = 91/122 (74%), Gaps = 0/122 (0%)
Query 2 KEEGIRNIAAAVNRAHKDTKFVVVVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVCL 61
KEE + IA ++ + T+ +++VLE MAGQ N IG FE+L+ IID V+ K RVGVC+
Sbjct 235 KEECMTTIAETIDFVVEKTENIILVLETMAGQGNSIGGTFEELKFIIDKVKVKSRVGVCI 294
Query 62 DTCHLFAAGYDIRTAEKFEKVMEDFDSIVGYKFLKGMHLNDSKSELSSGLDRHELLGKGH 121
DTCH+FA GYDIRT + +E+VM++F +VG+ +LK +H+NDSK ++ S LDRHE +G+G
Sbjct 295 DTCHIFAGGYDIRTQKAYEEVMKNFGEVVGWNYLKAIHINDSKGDVGSKLDRHEHIGQGK 354
Query 122 LG 123
+G
Sbjct 355 IG 356
> ath:AT4G20060 EMB1895 (EMBRYO DEFECTIVE 1895); binding; K13144
integrator complex subunit 7
Length=1134
Score = 29.6 bits (65), Expect = 2.4, Method: Composition-based stats.
Identities = 11/31 (35%), Positives = 20/31 (64%), Gaps = 0/31 (0%)
Query 21 KFVVVVLENMAGQKNVIGSKFEDLRDIIDLV 51
KF++V LEN+ G N++ +E ++ I + V
Sbjct 510 KFLIVFLENLEGDDNLLSEIYEKVKHITEFV 540
> mmu:16179 Irak1, AA408924, IRAK, IRAK-1, IRAK1-S, Il1rak, Plpk,
mPLK; interleukin-1 receptor-associated kinase 1 (EC:2.7.11.1);
K04730 interleukin-1 receptor-associated kinase 1 [EC:2.7.11.1]
Length=750
Score = 29.6 bits (65), Expect = 2.8, Method: Composition-based stats.
Identities = 24/64 (37%), Positives = 38/64 (59%), Gaps = 8/64 (12%)
Query 3 EEGIRNIAAAVNRAHKDT-KFVVVVLENMAGQKNV--IGSKFEDLRDIIDLVEDKDRVGV 59
EE I+ AV+ DT F VV+LE +AGQ+ V G+K + L+D+I+ ++ + GV
Sbjct 394 EEYIKTGRLAVD---TDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIE--DEAEEAGV 448
Query 60 CLDT 63
L +
Sbjct 449 TLKS 452
> eco:b4003 zraS, ECK3995, hydH, JW3967; sensory histidine kinase
in two-component regulatory system with ZraR; K07709 two-component
system, NtrC family, sensor histidine kinase HydH
[EC:2.7.13.3]
Length=465
Score = 29.3 bits (64), Expect = 3.5, Method: Composition-based stats.
Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 0/36 (0%)
Query 26 VLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVCL 61
+LE MAGQ V+ D + II L D D+VG L
Sbjct 80 LLEEMAGQPGVLWFAVTDAQGIIILHSDPDKVGRAL 115
> pfa:PF14_0613 conserved Plasmodium membrane protein, unknown
function
Length=3251
Score = 28.9 bits (63), Expect = 3.8, Method: Composition-based stats.
Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 0/39 (0%)
Query 25 VVLENMAGQKNVIGSKFEDLRDIIDLVEDKDRVGVCLDT 63
+ LE+ K++ K D++D+ID+ +DK + L T
Sbjct 2559 ITLEDKENMKDIYNEKISDIKDVIDIRKDKQDISTTLCT 2597
> ath:AT3G10320 transferase, transferring glycosyl groups
Length=494
Score = 28.9 bits (63), Expect = 4.5, Method: Composition-based stats.
Identities = 15/36 (41%), Positives = 25/36 (69%), Gaps = 5/36 (13%)
Query 77 EKFEK---VMEDFDSIV--GYKFLKGMHLNDSKSEL 107
EK++K +++D +SI G++F KG++LND K L
Sbjct 441 EKYDKDDPILKDPNSITKKGWQFTKGIYLNDQKVRL 476
Lambda K H
0.319 0.138 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2059772308
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40