bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: kyva
112,920 sequences; 47,500,486 total letters
Query= Eten_4453_orf4
Length=92
Score E
Sequences producing significant alignments: (Bits) Value
HsMi003 51.6 3e-07
YMi004 41.6 4e-04
DmMi003 38.5 0.003
SPMi001 33.1 0.12
AtMi114 28.9 2.7
> HsMi003
Length=513
Score = 51.6 bits (122), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 31/91 (34%), Positives = 39/91 (42%), Gaps = 0/91 (0%)
Query 1 FSFYGGIFSLFPPFSGLFFHGTRAQNPFRIFFWGGKKNFFPQTFFGIFGQPWGIFRLPGG 60
F+ GG FP FSG T A+ F I F G FFPQ F G+ G P P
Sbjct 387 FAIMGGFIHWFPLFSGYTLDQTYAKIHFTIMFIGVNLTFFPQHFLGLSGMPRRYSDYPDA 446
Query 61 FPNWKYFFSKGLFFFLNGGFIKGFLLWGSFC 91
+ W S G F L + F++W +F
Sbjct 447 YTTWNILSSVGSFISLTAVMLMIFMIWEAFA 477
> YMi004
Length=534
Score = 41.6 bits (96), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 30/77 (38%), Positives = 33/77 (42%), Gaps = 7/77 (9%)
Query 5 GGIFSLF-------PPFSGLFFHGTRAQNPFRIFFWGGKKNFFPQTFFGIFGQPWGIFRL 57
G IFSLF P GL ++ AQ F + F G FFP F GI G P I
Sbjct 384 GAIFSLFAGYYYWSPQILGLNYNEKLAQIQFWLIFIGANVIFFPMHFLGINGMPRRIPDY 443
Query 58 PGGFPNWKYFFSKGLFF 74
P F W Y S G F
Sbjct 444 PDAFAGWNYVASIGSFI 460
> DmMi003
Length=511
Score = 38.5 bits (88), Expect = 0.003, Method: Compositional matrix adjust.
Identities = 26/91 (28%), Positives = 37/91 (40%), Gaps = 0/91 (0%)
Query 1 FSFYGGIFSLFPPFSGLFFHGTRAQNPFRIFFWGGKKNFFPQTFFGIFGQPWGIFRLPGG 60
F+ G +P F+GL + ++ F I F G FFPQ F G+ G P P
Sbjct 385 FAIMAGFIHWYPLFTGLTLNNKWLKSHFIIMFIGVNLTFFPQHFLGLAGMPRRYSDYPDA 444
Query 61 FPNWKYFFSKGLFFFLNGGFIKGFLLWGSFC 91
+ W + G L G F++W S
Sbjct 445 YTTWNIVSTIGSTISLLGILFFFFIIWESLV 475
> SPMi001
Length=537
Score = 33.1 bits (74), Expect = 0.12, Method: Compositional matrix adjust.
Identities = 24/80 (30%), Positives = 34/80 (42%), Gaps = 0/80 (0%)
Query 12 PPFSGLFFHGTRAQNPFRIFFWGGKKNFFPQTFFGIFGQPWGIFRLPGGFPNWKYFFSKG 71
P GL ++ T A F I F G F PQ F G+ G P I P F W + S G
Sbjct 404 PKMFGLMYNETLASIQFWILFIGVNIVFGPQHFLGLNGMPRRIPDYPEAFVGWNFVSSIG 463
Query 72 LFFFLNGGFIKGFLLWGSFC 91
+ F+ ++++ F
Sbjct 464 SVISILSLFLFMYVMYDQFT 483
> AtMi114
Length=527
Score = 28.9 bits (63), Expect = 2.7, Method: Compositional matrix adjust.
Identities = 25/81 (30%), Positives = 33/81 (40%), Gaps = 7/81 (8%)
Query 5 GGIFSLFPPF---SGLFFHGTRAQNPFRIFFW----GGKKNFFPQTFFGIFGQPWGIFRL 57
G +F+LF F G F T + +I FW G FFP F G+ G P I
Sbjct 386 GAVFALFAGFYYWVGKIFGRTYPETLGQIHFWITFFGVNLTFFPMHFLGLSGMPRRIPDY 445
Query 58 PGGFPNWKYFFSKGLFFFLNG 78
P + W S G + + G
Sbjct 446 PDAYAGWNALSSFGSYISVVG 466
Lambda K H
0.338 0.160 0.611
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 1171209254
Database: kyva
Posted date: Jul 3, 2009 9:03 AM
Number of letters in database: 47,500,486
Number of sequences in database: 112,920
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40