bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_2058_orf2
Length=244
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_104770 ubiquitin-conjugating enzyme E2, putative (E... 250 3e-66
cpv:cgd3_2220 ubiquitin-conjugating enzyme ; K10580 ubiquitin-... 228 2e-59
pfa:PFE1350c ubiquitin conjugating enzyme, putative; K10580 ub... 227 3e-59
bbo:BBOV_IV007110 23.m05993; ubiquitin-conjugating enzyme E2; ... 213 4e-55
tpv:TP01_0508 ubiquitin-protein ligase; K10580 ubiquitin-conju... 203 4e-52
ath:AT1G16890 UBC36; ubiquitin-conjugating enzyme, putative; K... 198 2e-50
mmu:100046119 Mcg1038069; ubiquitin-conjugating enzyme E2 N-li... 195 1e-49
dre:393313 ube2nb, MGC63901, ube2nl, zgc:63901; ubiquitin-conj... 194 2e-49
dre:406807 ube2na, ube2n, wu:fb11b03, wu:fc08b06, zgc:55726; u... 194 2e-49
mmu:93765 Ube2n, 1500026J17Rik, AL022654, BB101821, UBC13; ubi... 193 6e-49
hsa:7334 UBE2N, MGC131857, MGC8489, UBC13, UbcH-ben; ubiquitin... 193 6e-49
xla:380524 ube2n, MGC64428; ubiquitin-conjugating enzyme E2N (... 188 1e-47
cel:Y54G2A.31 ubc-13; UBiquitin Conjugating enzyme family memb... 187 3e-47
sce:YDR092W UBC13; Ubc13p (EC:6.3.2.19); K10580 ubiquitin-conj... 176 1e-43
hsa:389898 UBE2NL, Li174; ubiquitin-conjugating enzyme E2N-lik... 170 4e-42
mmu:100041224 Gm3213; predicted gene 3213 154 3e-37
cpv:cgd4_210 Ubc1p like ubiquitin-conjugating enzyme E2 fused ... 150 5e-36
ath:AT1G78870 UBC35; UBC35 (UBIQUITIN-CONJUGATING ENZYME 35); ... 148 2e-35
ath:AT5G50870 UBC27; UBC27 (ubiquitin-conjugating enzyme 27); ... 141 2e-33
xla:379797 ube2t, MGC53460, hspc150; ubiquitin-conjugating enz... 138 2e-32
tpv:TP01_0732 ubiquitin conjugating enzyme (EC:6.3.2.19); K046... 138 2e-32
tgo:TGME49_119870 ubiquitin-conjugating enzyme domain-containi... 136 6e-32
hsa:29089 UBE2T, PIG50; ubiquitin-conjugating enzyme E2T (puta... 135 2e-31
sce:YDR177W UBC1; Ubc1p (EC:6.3.2.19); K04649 ubiquitin-conjug... 133 5e-31
mmu:67196 Ube2t, 2700084L22Rik, C80607; ubiquitin-conjugating ... 132 9e-31
pfa:PF13_0301 ubiquitin conjugating enzyme, putative; K04649 u... 132 2e-30
dre:30100 ube2k, hip2, zgc:103472; ubiquitin-conjugating enzym... 130 3e-30
sce:YBR082C UBC4; Ubc4p (EC:6.3.2.19); K06689 ubiquitin-conjug... 130 4e-30
ath:AT5G41700 UBC8; UBC8 (UBIQUITIN CONJUGATING ENZYME 8); pro... 129 7e-30
bbo:BBOV_IV010700 23.m05926; ubiquitin-conjugating enzyme; K04... 129 7e-30
ath:AT3G08690 UBC11; UBC11 (UBIQUITIN-CONJUGATING ENZYME 11); ... 129 8e-30
mmu:53323 Ube2k, AW492011, D5Ertd601e, E2-25k, Hip2, Hypg, Lig... 129 1e-29
pfa:PFL0190w ubiquitin conjugating enzyme E2, putative (EC:6.3... 128 1e-29
sce:YDR059C UBC5; Ubc5p (EC:6.3.2.19); K06689 ubiquitin-conjug... 128 1e-29
ath:AT1G64230 UBC28; ubiquitin-conjugating enzyme, putative; K... 128 2e-29
xla:379982 ube2k, MGC52694, e2-25k, hip2, hypg, lig; ubiquitin... 128 2e-29
tpv:TP03_0448 ubiquitin-conjugating enzyme (EC:6.3.2.19); K066... 127 3e-29
ath:AT2G16740 UBC29; UBC29 (ubiquitin-conjugating enzyme 29); ... 127 4e-29
ath:AT4G27960 UBC9; UBC9 (UBIQUITIN CONJUGATING ENZYME 9); ubi... 127 5e-29
bbo:BBOV_IV002040 21.m02810; ubiquitin-conjugating enzyme E2 (... 126 6e-29
dre:100001914 ube2d4, MGC162263, zgc:162263; ubiquitin-conjuga... 126 9e-29
hsa:51619 UBE2D4, FLJ32004, HBUCE1; ubiquitin-conjugating enzy... 126 9e-29
dre:324015 ube2d1, wu:fc16h06, zgc:73096; ubiquitin-conjugatin... 125 1e-28
dre:768152 zgc:153687 (EC:6.3.2.-); K13960 ubiquitin-conjugati... 125 1e-28
xla:495381 ube2d1, e2(17)kb1, sft, ubc4/5, ubch5, ubch5a; ubiq... 125 1e-28
xla:446340 ube2d2, MGC81261; ubiquitin-conjugating enzyme E2D ... 125 1e-28
dre:393934 MGC55886, Ube2d2, zgc:77149; zgc:55886 (EC:6.3.2.19... 125 2e-28
xla:403384 ube2d3, ube2d2, ube2d3.1, xubc4; ubiquitin-conjugat... 125 2e-28
mmu:56550 Ube2d2, 1500034D03Rik, Ubc2e, ubc4; ubiquitin-conjug... 125 2e-28
hsa:7322 UBE2D2, E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH5B; ubiqu... 125 2e-28
> tgo:TGME49_104770 ubiquitin-conjugating enzyme E2, putative
(EC:6.3.2.19); K10580 ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=155
Score = 250 bits (639), Expect = 3e-66, Method: Compositional matrix adjust.
Identities = 114/136 (83%), Positives = 125/136 (91%), Gaps = 0/136 (0%)
Query 109 PGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNI 168
PGI A P+P+NYR+FKI+M GP GTPYEGG+Y+LELFLPE YPMEPPKVRFLTKIYHPNI
Sbjct 20 PGITAQPEPNNYRHFKILMGGPVGTPYEGGQYKLELFLPEAYPMEPPKVRFLTKIYHPNI 79
Query 169 DALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERA 228
DALGRICLDILKDKWSPALQIRTVLLS+QALLSAPEPDDPLDTRVADHFKN+RQ AE A
Sbjct 80 DALGRICLDILKDKWSPALQIRTVLLSIQALLSAPEPDDPLDTRVADHFKNNRQDAELMA 139
Query 229 RQWREMYAGDETITLD 244
RQW EMYAG ++I +D
Sbjct 140 RQWNEMYAGQQSIVID 155
> cpv:cgd3_2220 ubiquitin-conjugating enzyme ; K10580 ubiquitin-conjugating
enzyme E2 N [EC:6.3.2.19]
Length=150
Score = 228 bits (580), Expect = 2e-59, Method: Compositional matrix adjust.
Identities = 103/129 (79%), Positives = 112/129 (86%), Gaps = 0/129 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
+PGI A PD NYRYFKI+M GP GTPYEGG Y+LELFLPE YPMEPPKVRFLTKIYHPN
Sbjct 19 SPGINAEPDEDNYRYFKIMMMGPEGTPYEGGSYKLELFLPEHYPMEPPKVRFLTKIYHPN 78
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
ID LGRICLDILKDKWSPALQIRTVLLS+QALLS+PEP+DPLDT VADHFKN+ AE
Sbjct 79 IDKLGRICLDILKDKWSPALQIRTVLLSIQALLSSPEPNDPLDTVVADHFKNNLLDAEST 138
Query 228 ARQWREMYA 236
AR+W +YA
Sbjct 139 AREWNRLYA 147
> pfa:PFE1350c ubiquitin conjugating enzyme, putative; K10580
ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=152
Score = 227 bits (578), Expect = 3e-59, Method: Compositional matrix adjust.
Identities = 101/133 (75%), Positives = 115/133 (86%), Gaps = 0/133 (0%)
Query 109 PGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNI 168
PGI A P P NYR+F I++ GP GTPYEGG Y+LELFLPEQYPMEPPKVRFLTKIYHPNI
Sbjct 20 PGIMAVPVPENYRHFNILINGPDGTPYEGGTYKLELFLPEQYPMEPPKVRFLTKIYHPNI 79
Query 169 DALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERA 228
D LGRICLDILKDKWSPALQIRTVLLS+QALLS+PEPDDPLD++VA+HFK D+ AE A
Sbjct 80 DKLGRICLDILKDKWSPALQIRTVLLSIQALLSSPEPDDPLDSKVAEHFKQDKNDAEHVA 139
Query 229 RQWREMYAGDETI 241
RQW ++YA + +
Sbjct 140 RQWNKIYANNNVL 152
> bbo:BBOV_IV007110 23.m05993; ubiquitin-conjugating enzyme E2;
K10580 ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=151
Score = 213 bits (543), Expect = 4e-55, Method: Compositional matrix adjust.
Identities = 102/134 (76%), Positives = 111/134 (82%), Gaps = 0/134 (0%)
Query 103 LSLHAAPGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTK 162
L+ + PGI+A +P N R+F I M GP TPYEGG Y+LELFLPEQYPMEPPKVRFLT
Sbjct 13 LANDSPPGIKAELNPGNNRHFYIRMLGPDSTPYEGGVYKLELFLPEQYPMEPPKVRFLTN 72
Query 163 IYHPNIDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQ 222
IYHPNID LGRICLDILKDKWSPALQIRTVLLSVQ+LLSAPEPDDPLDT VADHFK D+
Sbjct 73 IYHPNIDKLGRICLDILKDKWSPALQIRTVLLSVQSLLSAPEPDDPLDTSVADHFKADKA 132
Query 223 AAEERARQWREMYA 236
AE AR+W E YA
Sbjct 133 DAERLAREWNEKYA 146
> tpv:TP01_0508 ubiquitin-protein ligase; K10580 ubiquitin-conjugating
enzyme E2 N [EC:6.3.2.19]
Length=152
Score = 203 bits (517), Expect = 4e-52, Method: Compositional matrix adjust.
Identities = 96/134 (71%), Positives = 108/134 (80%), Gaps = 0/134 (0%)
Query 103 LSLHAAPGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTK 162
L+ + PGI+A N R+FKI M GP TPYEGG Y LELFLPE YPM+PPKVRFLT
Sbjct 13 LATDSPPGIKAELFVGNDRHFKIHMQGPDSTPYEGGVYILELFLPEHYPMDPPKVRFLTS 72
Query 163 IYHPNIDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQ 222
IYHPNID +GRICLDILKDKWSPALQIRTVLLS+QALLSAPEPDDPLD VA+HFK DR
Sbjct 73 IYHPNIDKIGRICLDILKDKWSPALQIRTVLLSIQALLSAPEPDDPLDASVANHFKTDRV 132
Query 223 AAEERARQWREMYA 236
AE+ AR+W + +A
Sbjct 133 GAEKVAREWNQTFA 146
> ath:AT1G16890 UBC36; ubiquitin-conjugating enzyme, putative;
K10580 ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=153
Score = 198 bits (503), Expect = 2e-50, Method: Compositional matrix adjust.
Identities = 88/130 (67%), Positives = 104/130 (80%), Gaps = 0/130 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
APGI A+P N RYF +++ GP +PYEGG ++LELFLPE+YPM PKVRFLTKIYHPN
Sbjct 22 APGISASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPN 81
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
ID LGRICLDILKDKWSPALQIRTVLLS+QALLSAP PDDPL +A H+K++ A E
Sbjct 82 IDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET 141
Query 228 ARQWREMYAG 237
A++W +YA
Sbjct 142 AKEWTRLYAS 151
> mmu:100046119 Mcg1038069; ubiquitin-conjugating enzyme E2 N-like;
K10580 ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=203
Score = 195 bits (496), Expect = 1e-49, Method: Compositional matrix adjust.
Identities = 87/134 (64%), Positives = 105/134 (78%), Gaps = 0/134 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI+A PD SN RYF +++ GP +P+EGG ++LELFLPE+YPM PKVRF+TKIYHPN
Sbjct 20 VPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPN 79
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
+D LGRICLDILKDKWSPALQIRTVLLS+QALLSAP PDDPL VA+ +K + A E
Sbjct 80 VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139
Query 228 ARQWREMYAGDETI 241
AR W +YA + +
Sbjct 140 ARAWTRLYAMNNIL 153
> dre:393313 ube2nb, MGC63901, ube2nl, zgc:63901; ubiquitin-conjugating
enzyme E2Nb (EC:6.3.2.-); K10580 ubiquitin-conjugating
enzyme E2 N [EC:6.3.2.19]
Length=154
Score = 194 bits (494), Expect = 2e-49, Method: Compositional matrix adjust.
Identities = 87/131 (66%), Positives = 105/131 (80%), Gaps = 0/131 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI+A PD N RYF +++ GP +P+EGG ++LELFLPE+YPM PKVRF+TKIYHPN
Sbjct 20 VPGIKAEPDEGNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPN 79
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
+D LGRICLDILKDKWSPALQIRTVLLS+QALLSAP PDDPL VA+ +K++ A E
Sbjct 80 VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIET 139
Query 228 ARQWREMYAGD 238
AR W +YAG+
Sbjct 140 ARTWTRLYAGN 150
> dre:406807 ube2na, ube2n, wu:fb11b03, wu:fc08b06, zgc:55726;
ubiquitin-conjugating enzyme E2Na (EC:6.3.2.19); K10580 ubiquitin-conjugating
enzyme E2 N [EC:6.3.2.19]
Length=154
Score = 194 bits (493), Expect = 2e-49, Method: Compositional matrix adjust.
Identities = 87/131 (66%), Positives = 104/131 (79%), Gaps = 0/131 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI+A PD N RYF +++ GP +P+EGG ++LELFLPE+YPM PKVRF+TKIYHPN
Sbjct 20 VPGIKAEPDEGNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPN 79
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
+D LGRICLDILKDKWSPALQIRTVLLS+QALLSAP PDDPL VA+ +K + A E
Sbjct 80 VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139
Query 228 ARQWREMYAGD 238
AR W +YAG+
Sbjct 140 ARTWTRLYAGN 150
> mmu:93765 Ube2n, 1500026J17Rik, AL022654, BB101821, UBC13; ubiquitin-conjugating
enzyme E2N (EC:6.3.2.19); K10580 ubiquitin-conjugating
enzyme E2 N [EC:6.3.2.19]
Length=152
Score = 193 bits (490), Expect = 6e-49, Method: Compositional matrix adjust.
Identities = 87/129 (67%), Positives = 103/129 (79%), Gaps = 0/129 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI+A PD SN RYF +++ GP +P+EGG ++LELFLPE+YPM PKVRF+TKIYHPN
Sbjct 20 VPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPN 79
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
+D LGRICLDILKDKWSPALQIRTVLLS+QALLSAP PDDPL VA+ +K + A E
Sbjct 80 VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139
Query 228 ARQWREMYA 236
AR W +YA
Sbjct 140 ARAWTRLYA 148
> hsa:7334 UBE2N, MGC131857, MGC8489, UBC13, UbcH-ben; ubiquitin-conjugating
enzyme E2N (UBC13 homolog, yeast) (EC:6.3.2.19);
K10580 ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=152
Score = 193 bits (490), Expect = 6e-49, Method: Compositional matrix adjust.
Identities = 87/129 (67%), Positives = 103/129 (79%), Gaps = 0/129 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI+A PD SN RYF +++ GP +P+EGG ++LELFLPE+YPM PKVRF+TKIYHPN
Sbjct 20 VPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPN 79
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
+D LGRICLDILKDKWSPALQIRTVLLS+QALLSAP PDDPL VA+ +K + A E
Sbjct 80 VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139
Query 228 ARQWREMYA 236
AR W +YA
Sbjct 140 ARAWTRLYA 148
> xla:380524 ube2n, MGC64428; ubiquitin-conjugating enzyme E2N
(UBC13 homolog) (EC:6.3.2.19); K10580 ubiquitin-conjugating
enzyme E2 N [EC:6.3.2.19]
Length=152
Score = 188 bits (478), Expect = 1e-47, Method: Compositional matrix adjust.
Identities = 85/129 (65%), Positives = 102/129 (79%), Gaps = 0/129 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI+A PD +N R+F + + GP +P+EGG ++LELFLPE+YPM PKVRF+TKIYHPN
Sbjct 20 VPGIKAEPDENNARFFHVAIAGPQDSPFEGGSFKLELFLPEEYPMAAPKVRFMTKIYHPN 79
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
+D LGRICLDILKDKWSPALQIRTVLLS+QALLSAP PDDPL VA+ +K + A E
Sbjct 80 VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139
Query 228 ARQWREMYA 236
AR W +YA
Sbjct 140 ARAWTRLYA 148
> cel:Y54G2A.31 ubc-13; UBiquitin Conjugating enzyme family member
(ubc-13); K10580 ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=151
Score = 187 bits (475), Expect = 3e-47, Method: Compositional matrix adjust.
Identities = 86/129 (66%), Positives = 102/129 (79%), Gaps = 0/129 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI A PD SN RYF +++ GP +P+ GG ++LELFLPE+YPM PKVRF+TKIYHPN
Sbjct 21 VPGISANPDESNARYFHVMIAGPDDSPFAGGVFKLELFLPEEYPMAAPKVRFMTKIYHPN 80
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
ID LGRICLDILKDKWSPALQIRTVLLS+QALLSAP P+DPL T VA+ +K + A +
Sbjct 81 IDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPEDPLATDVAEQWKTNEAEAIKT 140
Query 228 ARQWREMYA 236
A+QW YA
Sbjct 141 AKQWTMNYA 149
> sce:YDR092W UBC13; Ubc13p (EC:6.3.2.19); K10580 ubiquitin-conjugating
enzyme E2 N [EC:6.3.2.19]
Length=153
Score = 176 bits (445), Expect = 1e-43, Method: Compositional matrix adjust.
Identities = 79/129 (61%), Positives = 99/129 (76%), Gaps = 0/129 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
PGI A P N RYF++ + GP +PYE G + LEL+LP+ YPME PKVRFLTKIYHPN
Sbjct 20 VPGITAEPHDDNLRYFQVTIEGPEQSPYEDGIFELELYLPDDYPMEAPKVRFLTKIYHPN 79
Query 168 IDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEER 227
ID LGRICLD+LK WSPALQIRTVLLS+QALL++P P+DPL VA+ + + Q A+ +
Sbjct 80 IDRLGRICLDVLKTNWSPALQIRTVLLSIQALLASPNPNDPLANDVAEDWIKNEQGAKAK 139
Query 228 ARQWREMYA 236
AR+W ++YA
Sbjct 140 AREWTKLYA 148
> hsa:389898 UBE2NL, Li174; ubiquitin-conjugating enzyme E2N-like
(EC:6.3.2.-); K10580 ubiquitin-conjugating enzyme E2 N [EC:6.3.2.19]
Length=153
Score = 170 bits (431), Expect = 4e-42, Method: Compositional matrix adjust.
Identities = 79/132 (59%), Positives = 98/132 (74%), Gaps = 1/132 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAG-TPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHP 166
PGI+A PD SN RYF +++ G + +P+EGG ++ EL L E+YPM PKVRF+TKIYHP
Sbjct 20 VPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKVRFMTKIYHP 79
Query 167 NIDALGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEE 226
N+D L RI LDILKDKWSPALQIRTVLLS+QALL+AP PDDPL V + +K + A E
Sbjct 80 NVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVEQWKTNEAQAIE 139
Query 227 RARQWREMYAGD 238
AR W +YA +
Sbjct 140 TARAWTRLYAMN 151
> mmu:100041224 Gm3213; predicted gene 3213
Length=173
Score = 154 bits (389), Expect = 3e-37, Method: Compositional matrix adjust.
Identities = 76/162 (46%), Positives = 99/162 (61%), Gaps = 6/162 (3%)
Query 79 HSGSAAAGSAAAAANPCSCCCCCC----LSLHAAPGIEAAPDPSNYRYFKIIMTGPAGTP 134
H+ +PC C L PGI A PD ++ YF +++ G +P
Sbjct 10 HASHGKPADTLLPRDPCWTGCNIKETQRLLAEPVPGITAEPDENSAHYFPVVIAGTQDSP 69
Query 135 YEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALGRICLDILKDKWSPALQIRTVLL 194
++GG ++LELFLPE+YPM PKV F+T+IYHPN++ L +I DILKDKWSPALQIRTVLL
Sbjct 70 FDGGTFKLELFLPEEYPMAAPKVCFMTEIYHPNVNKLEKI--DILKDKWSPALQIRTVLL 127
Query 195 SVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYA 236
S+Q LLSAP PDDPL VA+ +K + E W +YA
Sbjct 128 SIQPLLSAPNPDDPLANDVAEQWKTNEGQGIETVTAWTRLYA 169
> cpv:cgd4_210 Ubc1p like ubiquitin-conjugating enzyme E2 fused
to a UBA domain (UBC+UBA) ; K04649 ubiquitin-conjugating enzyme
(huntingtin interacting protein 2) [EC:6.3.2.19]
Length=197
Score = 150 bits (378), Expect = 5e-36, Method: Compositional matrix adjust.
Identities = 67/112 (59%), Positives = 89/112 (79%), Gaps = 1/112 (0%)
Query 126 IMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDA-LGRICLDILKDKWS 184
I+ GP GTPYEGG ++L++ +P++YP EPPKV+F+T+I+HPNI + G ICLDILKD WS
Sbjct 42 IIRGPIGTPYEGGIFQLDIIVPKEYPYEPPKVKFITRIWHPNISSQTGAICLDILKDAWS 101
Query 185 PALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYA 236
PAL +RTV+LS+QALLS+PEP+DP D VA +K+D Q E A+ W +MYA
Sbjct 102 PALTLRTVMLSIQALLSSPEPNDPQDALVASLYKSDYQEYIETAKSWTQMYA 153
> ath:AT1G78870 UBC35; UBC35 (UBIQUITIN-CONJUGATING ENZYME 35);
protein binding / ubiquitin-protein ligase
Length=112
Score = 148 bits (373), Expect = 2e-35, Method: Compositional matrix adjust.
Identities = 66/87 (75%), Positives = 74/87 (85%), Gaps = 0/87 (0%)
Query 108 APGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPN 167
APGI A+P N RYF +++ GP +PYEGG ++LELFLPE+YPM PKVRFLTKIYHPN
Sbjct 22 APGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPN 81
Query 168 IDALGRICLDILKDKWSPALQIRTVLL 194
ID LGRICLDILKDKWSPALQIRTVLL
Sbjct 82 IDKLGRICLDILKDKWSPALQIRTVLL 108
> ath:AT5G50870 UBC27; UBC27 (ubiquitin-conjugating enzyme 27);
ubiquitin-protein ligase; K04649 ubiquitin-conjugating enzyme
(huntingtin interacting protein 2) [EC:6.3.2.19]
Length=192
Score = 141 bits (355), Expect = 2e-33, Method: Compositional matrix adjust.
Identities = 64/133 (48%), Positives = 88/133 (66%), Gaps = 1/133 (0%)
Query 110 GIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNID 169
GI P N + GP GTPYEGG +++++ +P+ YP EPPK++F TK++HPNI
Sbjct 22 GIRVCPKSDNLTRLTGTIPGPIGTPYEGGTFQIDITMPDGYPFEPPKMQFSTKVWHPNIS 81
Query 170 AL-GRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERA 228
+ G ICLDILKD+WSPAL ++T L+S+QALLSAPEP DP D VA+ + + Q A
Sbjct 82 SQSGAICLDILKDQWSPALTLKTALVSIQALLSAPEPKDPQDAVVAEQYMKNYQVFVSTA 141
Query 229 RQWREMYAGDETI 241
R W E +A ++
Sbjct 142 RYWTETFAKKSSL 154
> xla:379797 ube2t, MGC53460, hspc150; ubiquitin-conjugating enzyme
E2T (putative) (EC:6.3.2.19); K13960 ubiquitin-conjugating
enzyme E2 T [EC:6.3.2.19]
Length=192
Score = 138 bits (348), Expect = 2e-32, Method: Compositional matrix adjust.
Identities = 67/139 (48%), Positives = 88/139 (63%), Gaps = 4/139 (2%)
Query 102 CLSLHAAPGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLT 161
L+ PG+ + SN + + G +G+PYEGG + LE+ +PE+YP EPPK+RFLT
Sbjct 13 LLNKEPPPGVICWQNESNMDDLRAQIIGGSGSPYEGGIFNLEIIVPERYPFEPPKIRFLT 72
Query 162 KIYHPNIDALGRICLDIL----KDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHF 217
IYHPNID+ GRICLDIL K W PAL I TVL S+Q L+S P PDDPL ++ F
Sbjct 73 PIYHPNIDSAGRICLDILKLPPKGAWRPALNISTVLTSIQLLMSEPNPDDPLMADISSEF 132
Query 218 KNDRQAAEERARQWREMYA 236
K +R A++W E +A
Sbjct 133 KYNRAVFFSNAKKWTEKHA 151
> tpv:TP01_0732 ubiquitin conjugating enzyme (EC:6.3.2.19); K04649
ubiquitin-conjugating enzyme (huntingtin interacting protein
2) [EC:6.3.2.19]
Length=177
Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust.
Identities = 66/127 (51%), Positives = 88/127 (69%), Gaps = 1/127 (0%)
Query 111 IEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDA 170
+EA +N +K + GP GTPYEGG + L++ +PE YP PP ++F TKI+HPNI +
Sbjct 24 VEAYVVDNNIFKWKGHILGPPGTPYEGGHFTLDISIPEDYPYSPPVIKFETKIWHPNISS 83
Query 171 -LGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERAR 229
G ICLDILK +WSPAL IRT L+S+QALLSAPEPDDP D +VA+ +K + + E A+
Sbjct 84 ETGAICLDILKSEWSPALTIRTALISIQALLSAPEPDDPQDAQVANMYKRNYKEFENTAK 143
Query 230 QWREMYA 236
W +A
Sbjct 144 LWTSTFA 150
> tgo:TGME49_119870 ubiquitin-conjugating enzyme domain-containing
protein (EC:6.3.2.19); K06689 ubiquitin-conjugating enzyme
E2 D/E [EC:6.3.2.19]
Length=345
Score = 136 bits (343), Expect = 6e-32, Method: Compositional matrix adjust.
Identities = 61/124 (49%), Positives = 83/124 (66%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY GG + L + P YP +PPKV F TKIYHPNI++ G
Sbjct 221 AGPVGDDMFHWQATIMGPEDSPYSGGVFFLNIHFPSDYPFKPPKVNFTTKIYHPNINSQG 280
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILKD+WSPAL I VLLS+ +LL+ P PDDPL +A +K+DR ++ AR+W
Sbjct 281 AICLDILKDQWSPALTISKVLLSISSLLTDPNPDDPLVPEIAHLYKSDRMRYDQTAREWS 340
Query 233 EMYA 236
+ YA
Sbjct 341 QKYA 344
> hsa:29089 UBE2T, PIG50; ubiquitin-conjugating enzyme E2T (putative)
(EC:6.3.2.19); K13960 ubiquitin-conjugating enzyme E2
T [EC:6.3.2.19]
Length=197
Score = 135 bits (339), Expect = 2e-31, Method: Compositional matrix adjust.
Identities = 70/152 (46%), Positives = 91/152 (59%), Gaps = 9/152 (5%)
Query 102 CLSLHAAPGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLT 161
L+ PGI D + + G A TPYE G ++LE+ +PE+YP EPP++RFLT
Sbjct 13 MLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLT 72
Query 162 KIYHPNIDALGRICLDIL----KDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHF 217
IYHPNID+ GRICLD+L K W P+L I TVL S+Q L+S P PDDPL ++ F
Sbjct 73 PIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEF 132
Query 218 KNDRQAAEERARQWREMYA-----GDETITLD 244
K ++ A + ARQW E +A DE LD
Sbjct 133 KYNKPAFLKNARQWTEKHARQKQKADEEEMLD 164
> sce:YDR177W UBC1; Ubc1p (EC:6.3.2.19); K04649 ubiquitin-conjugating
enzyme (huntingtin interacting protein 2) [EC:6.3.2.19]
Length=215
Score = 133 bits (335), Expect = 5e-31, Method: Compositional matrix adjust.
Identities = 57/122 (46%), Positives = 87/122 (71%), Gaps = 1/122 (0%)
Query 118 SNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDAL-GRICL 176
S+ + K GP GTPYEGG++ +++ +P +YP +PPK++F TK+YHPNI ++ G ICL
Sbjct 30 SDIHHLKGTFLGPPGTPYEGGKFVVDIEVPMEYPFKPPKMQFDTKVYHPNISSVTGAICL 89
Query 177 DILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYA 236
DILK+ WSP + +++ L+S+QALL +PEP+DP D VA H+ DR++ + A W +YA
Sbjct 90 DILKNAWSPVITLKSALISLQALLQSPEPNDPQDAEVAQHYLRDRESFNKTAALWTRLYA 149
Query 237 GD 238
+
Sbjct 150 SE 151
> mmu:67196 Ube2t, 2700084L22Rik, C80607; ubiquitin-conjugating
enzyme E2T (putative) (EC:6.3.2.19); K13960 ubiquitin-conjugating
enzyme E2 T [EC:6.3.2.19]
Length=204
Score = 132 bits (333), Expect = 9e-31, Method: Compositional matrix adjust.
Identities = 65/139 (46%), Positives = 88/139 (63%), Gaps = 4/139 (2%)
Query 102 CLSLHAAPGIEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLT 161
L++ PGI + + + G A TPYE G + LE+ +PE+YP EPP+VRFLT
Sbjct 13 MLAIEPPPGITCWQEKDQVADLRAQILGGANTPYEKGVFTLEVIIPERYPFEPPQVRFLT 72
Query 162 KIYHPNIDALGRICLDIL----KDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHF 217
IYHPNID+ GRICLDIL K W P+L I TVL S+Q L++ P PDDPL ++ F
Sbjct 73 PIYHPNIDSSGRICLDILKLPPKGAWRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEF 132
Query 218 KNDRQAAEERARQWREMYA 236
K ++ A ++A+QW E +A
Sbjct 133 KYNKIAFLKKAKQWTEAHA 151
> pfa:PF13_0301 ubiquitin conjugating enzyme, putative; K04649
ubiquitin-conjugating enzyme (huntingtin interacting protein
2) [EC:6.3.2.19]
Length=202
Score = 132 bits (331), Expect = 2e-30, Method: Compositional matrix adjust.
Identities = 59/112 (52%), Positives = 79/112 (70%), Gaps = 1/112 (0%)
Query 126 IMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDA-LGRICLDILKDKWS 184
+ GP+GTPYEGG + L++ +P YP PPK++F TKI+HPNI + G ICLD+LK++WS
Sbjct 44 FIKGPSGTPYEGGHFILDITIPNDYPYNPPKIKFNTKIWHPNISSQTGAICLDVLKNEWS 103
Query 185 PALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYA 236
PAL IRT LLS+QALLS P+PDDP D VA +K + + A W + +A
Sbjct 104 PALTIRTALLSIQALLSDPQPDDPQDAEVAKMYKENYSLYLKTASVWTKTFA 155
> dre:30100 ube2k, hip2, zgc:103472; ubiquitin-conjugating enzyme
E2K (UBC1 homolog, yeast) (EC:6.3.2.19); K04649 ubiquitin-conjugating
enzyme (huntingtin interacting protein 2) [EC:6.3.2.19]
Length=200
Score = 130 bits (328), Expect = 3e-30, Method: Compositional matrix adjust.
Identities = 68/120 (56%), Positives = 90/120 (75%), Gaps = 1/120 (0%)
Query 119 NYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDAL-GRICLD 177
N+ K + GP TPYEGGRY+LE+ +PE YP PPKVRF+TKI+HPNI ++ G ICLD
Sbjct 35 NFTELKGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLD 94
Query 178 ILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYAG 237
ILKD+W+ A+ +RTVLLS+QALL+A EPDDP D VA+ +K + + ++ AR W +YAG
Sbjct 95 ILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWSHVYAG 154
> sce:YBR082C UBC4; Ubc4p (EC:6.3.2.19); K06689 ubiquitin-conjugating
enzyme E2 D/E [EC:6.3.2.19]
Length=148
Score = 130 bits (327), Expect = 4e-30, Method: Compositional matrix adjust.
Identities = 61/124 (49%), Positives = 81/124 (65%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GPA +PY GG + L + P YP +PPK+ F TKIYHPNI+A G
Sbjct 24 AGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANG 83
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILKD+WSPAL + VLLS+ +LL+ PDDPL +A +K DR E AR+W
Sbjct 84 NICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWT 143
Query 233 EMYA 236
+ YA
Sbjct 144 KKYA 147
> ath:AT5G41700 UBC8; UBC8 (UBIQUITIN CONJUGATING ENZYME 8); protein
binding / ubiquitin-protein ligase
Length=149
Score = 129 bits (325), Expect = 7e-30, Method: Compositional matrix adjust.
Identities = 60/126 (47%), Positives = 82/126 (65%), Gaps = 0/126 (0%)
Query 111 IEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDA 170
I A P + +++ + GPA +PY GG + + + P YP +PPKV F TK++HPNI++
Sbjct 22 IFAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINS 81
Query 171 LGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQ 230
G ICLDILK++WSPAL I VLLS+ +LL+ P PDDPL +A +K DR E AR
Sbjct 82 NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEATARN 141
Query 231 WREMYA 236
W + YA
Sbjct 142 WTQKYA 147
> bbo:BBOV_IV010700 23.m05926; ubiquitin-conjugating enzyme; K04649
ubiquitin-conjugating enzyme (huntingtin interacting protein
2) [EC:6.3.2.19]
Length=197
Score = 129 bits (325), Expect = 7e-30, Method: Compositional matrix adjust.
Identities = 62/127 (48%), Positives = 84/127 (66%), Gaps = 1/127 (0%)
Query 111 IEAAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDA 170
++A +N +K + GP TPYEGG + L + +P+ YP PPK+RF TKI+HPNI +
Sbjct 23 VDAHTVENNIFKWKGYIKGPIQTPYEGGLFILNIDIPDDYPYNPPKIRFETKIWHPNISS 82
Query 171 -LGRICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERAR 229
G ICLDILK++WSPAL +RT LLS+QAL+S PEPDDP D VA ++ + E A+
Sbjct 83 ETGAICLDILKNEWSPALTLRTALLSIQALMSTPEPDDPQDAEVATMYQRNYDEFERTAK 142
Query 230 QWREMYA 236
W +A
Sbjct 143 LWTATFA 149
> ath:AT3G08690 UBC11; UBC11 (UBIQUITIN-CONJUGATING ENZYME 11);
ubiquitin-protein ligase; K06689 ubiquitin-conjugating enzyme
E2 D/E [EC:6.3.2.19]
Length=148
Score = 129 bits (325), Expect = 8e-30, Method: Compositional matrix adjust.
Identities = 59/124 (47%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY GG + + + P YP +PPKV F TK+YHPNI++ G
Sbjct 23 AGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILK++WSPAL I VLLS+ +LL+ P PDDPL +A +K DR E AR W
Sbjct 83 SICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYESTARSWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> mmu:53323 Ube2k, AW492011, D5Ertd601e, E2-25k, Hip2, Hypg, Lig;
ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) (EC:6.3.2.19);
K04649 ubiquitin-conjugating enzyme (huntingtin
interacting protein 2) [EC:6.3.2.19]
Length=200
Score = 129 bits (324), Expect = 1e-29, Method: Compositional matrix adjust.
Identities = 67/120 (55%), Positives = 90/120 (75%), Gaps = 1/120 (0%)
Query 119 NYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDAL-GRICLD 177
N+ + + GP TPYEGGRY+LE+ +PE YP PPKVRF+TKI+HPNI ++ G ICLD
Sbjct 35 NFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLD 94
Query 178 ILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYAG 237
ILKD+W+ A+ +RTVLLS+QALL+A EPDDP D VA+ +K + + ++ AR W +YAG
Sbjct 95 ILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAG 154
> pfa:PFL0190w ubiquitin conjugating enzyme E2, putative (EC:6.3.2.19);
K06689 ubiquitin-conjugating enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust.
Identities = 59/124 (47%), Positives = 78/124 (62%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PYE G Y L + P YP +PPK+ F TKIYHPNI+ G
Sbjct 23 AGPIGDDLFFWQATIMGPGDSPYENGVYFLNIKFPPDYPFKPPKIIFTTKIYHPNINTAG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILKD+WSPAL I VLLS+ +LL+ P DDPL +A +K DR + A+ W
Sbjct 83 AICLDILKDQWSPALTISKVLLSISSLLTDPNADDPLVPEIAHVYKTDRTKYHQTAKAWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> sce:YDR059C UBC5; Ubc5p (EC:6.3.2.19); K06689 ubiquitin-conjugating
enzyme E2 D/E [EC:6.3.2.19]
Length=148
Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 81/124 (65%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP+ +PY GG + L + P YP +PPKV F TKIYHPNI++ G
Sbjct 24 AGPVGDDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDYPFKPPKVNFTTKIYHPNINSSG 83
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILKD+WSPAL + VLLS+ +LL+ PDDPL +A +K D+ E A++W
Sbjct 84 NICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKEWT 143
Query 233 EMYA 236
+ YA
Sbjct 144 KKYA 147
> ath:AT1G64230 UBC28; ubiquitin-conjugating enzyme, putative;
K06689 ubiquitin-conjugating enzyme E2 D/E [EC:6.3.2.19]
Length=148
Score = 128 bits (322), Expect = 2e-29, Method: Compositional matrix adjust.
Identities = 57/124 (45%), Positives = 81/124 (65%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP+ +PY GG + + + P YP +PPKV F TK++HPN+++ G
Sbjct 23 AGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVNSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILK++WSPAL I VLLS+ +LL+ P PDDPL +A +K DR E AR W
Sbjct 83 SICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> xla:379982 ube2k, MGC52694, e2-25k, hip2, hypg, lig; ubiquitin-conjugating
enzyme E2K (UBC1 homolog) (EC:6.3.2.19); K04649
ubiquitin-conjugating enzyme (huntingtin interacting protein
2) [EC:6.3.2.19]
Length=200
Score = 128 bits (322), Expect = 2e-29, Method: Compositional matrix adjust.
Identities = 66/120 (55%), Positives = 90/120 (75%), Gaps = 1/120 (0%)
Query 119 NYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDAL-GRICLD 177
N+ + + GP TPYEGGR++LE+ +PE YP PPKVRF+TKI+HPNI ++ G ICLD
Sbjct 35 NFSELRGEIAGPPDTPYEGGRFQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLD 94
Query 178 ILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYAG 237
ILKD+W+ A+ +RTVLLS+QALL+A EPDDP D VA+ +K + + ++ AR W +YAG
Sbjct 95 ILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAG 154
> tpv:TP03_0448 ubiquitin-conjugating enzyme (EC:6.3.2.19); K06689
ubiquitin-conjugating enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 127 bits (319), Expect = 3e-29, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 79/124 (63%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP + Y+ G Y L + P YP +PPKV F TK+YHPNI+ G
Sbjct 23 AGPVGDDMFHWQATIMGPHNSLYQNGVYFLNIHFPSDYPFKPPKVAFTTKVYHPNINNNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILKD+WSPAL I VLLS+ +LL+ P PDDPL +A +K +R+ E R+W
Sbjct 83 AICLDILKDQWSPALTISKVLLSISSLLTDPNPDDPLVPEIAQIYKQNRKLYESTVREWV 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> ath:AT2G16740 UBC29; UBC29 (ubiquitin-conjugating enzyme 29);
ubiquitin-protein ligase; K06689 ubiquitin-conjugating enzyme
E2 D/E [EC:6.3.2.19]
Length=148
Score = 127 bits (319), Expect = 4e-29, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY GG + + + P YP +PPKV F TK++HPNI++ G
Sbjct 23 AGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDYPFKPPKVVFRTKVFHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILKD+WSPAL I VLLS+ +LL+ P PDDPL +A +K D+ E AR W
Sbjct 83 NICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHIYKTDKTKYEAMARSWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> ath:AT4G27960 UBC9; UBC9 (UBIQUITIN CONJUGATING ENZYME 9); ubiquitin-protein
ligase
Length=178
Score = 127 bits (318), Expect = 5e-29, Method: Compositional matrix adjust.
Identities = 57/124 (45%), Positives = 81/124 (65%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP+ +PY GG + + + P YP +PPKV F TK++HPNI++ G
Sbjct 53 AGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNG 112
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILK++WSPAL I VLLS+ +LL+ P PDDPL +A +K D+ E AR W
Sbjct 113 SICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDKNKYESTARTWT 172
Query 233 EMYA 236
+ YA
Sbjct 173 QKYA 176
> bbo:BBOV_IV002040 21.m02810; ubiquitin-conjugating enzyme E2
(EC:6.3.2.19); K06689 ubiquitin-conjugating enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 126 bits (317), Expect = 6e-29, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 78/124 (62%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP + Y+ G Y L + P YP +PPKV F TK+YHPNI+ G
Sbjct 23 AGPVGDDMFHWQATIMGPHNSLYQNGVYFLNIHFPSDYPFKPPKVAFTTKVYHPNINNNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDILKD+WSPAL I VLLS+ +LL+ P PDDPL +A +K +R+ E R W
Sbjct 83 AICLDILKDQWSPALTISKVLLSISSLLTDPNPDDPLVPEIAQLYKQNRKLYESTVRDWV 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> dre:100001914 ube2d4, MGC162263, zgc:162263; ubiquitin-conjugating
enzyme E2D 4 (putative) (EC:6.3.2.19); K06689 ubiquitin-conjugating
enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 126 bits (316), Expect = 9e-29, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F TKIYHPNI++ G
Sbjct 23 AGPVGEDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL + VLLS+ +LL P PDDPL +A +K DR+ AR+W
Sbjct 83 SICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> hsa:51619 UBE2D4, FLJ32004, HBUCE1; ubiquitin-conjugating enzyme
E2D 4 (putative) (EC:6.3.2.19); K06689 ubiquitin-conjugating
enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 126 bits (316), Expect = 9e-29, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F TKIYHPNI++ G
Sbjct 23 AGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL + VLLS+ +LL P PDDPL +A +K DR+ AR+W
Sbjct 83 SICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> dre:324015 ube2d1, wu:fc16h06, zgc:73096; ubiquitin-conjugating
enzyme E2D 1 (UBC4/5 homolog, yeast) (EC:6.3.2.-); K06689
ubiquitin-conjugating enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 125 bits (315), Expect = 1e-28, Method: Compositional matrix adjust.
Identities = 57/124 (45%), Positives = 82/124 (66%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP+ +PY+GG + L + P YP +PPKV F TKIYHPNI++ G
Sbjct 23 AGPVGDDLFHWQATIMGPSDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL + VLLS+ +LL P PDDPL +A +K+D++ AR+W
Sbjct 83 SICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAHIYKSDKEKYNRLAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> dre:768152 zgc:153687 (EC:6.3.2.-); K13960 ubiquitin-conjugating
enzyme E2 T [EC:6.3.2.19]
Length=194
Score = 125 bits (315), Expect = 1e-28, Method: Compositional matrix adjust.
Identities = 59/114 (51%), Positives = 77/114 (67%), Gaps = 4/114 (3%)
Query 127 MTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALGRICLDIL----KDK 182
+ G A TPYEGG + LE+ +PE+YP EPPK+RFLT IYHPNID GRICLD L K
Sbjct 38 IVGGANTPYEGGVFTLEINIPERYPFEPPKMRFLTPIYHPNIDNAGRICLDALKLPPKGA 97
Query 183 WSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWREMYA 236
W P+L I TVL S+Q L++ P PDDPL ++ FK ++ E+A++W +A
Sbjct 98 WRPSLNISTVLTSIQLLMAEPNPDDPLMADISSEFKYNKPLYLEKAKKWTAEHA 151
> xla:495381 ube2d1, e2(17)kb1, sft, ubc4/5, ubch5, ubch5a; ubiquitin-conjugating
enzyme E2D 1 (UBC4/5 homolog)
Length=147
Score = 125 bits (315), Expect = 1e-28, Method: Compositional matrix adjust.
Identities = 57/124 (45%), Positives = 81/124 (65%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F TKIYHPNI++ G
Sbjct 23 AGPVGDDLFHWQATIMGPTDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL + VLLS+ +LL P PDDPL +A +K+D++ AR+W
Sbjct 83 SICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRLAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> xla:446340 ube2d2, MGC81261; ubiquitin-conjugating enzyme E2D
2 (UBC4/5 homolog, yeast) (EC:6.3.2.-); K06689 ubiquitin-conjugating
enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 125 bits (315), Expect = 1e-28, Method: Compositional matrix adjust.
Identities = 57/124 (45%), Positives = 81/124 (65%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F TKIYHPNI++ G
Sbjct 23 AGPVGDDLFHWQATIMGPTDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL + VLLS+ +LL P PDDPL +A +K+D++ AR+W
Sbjct 83 SICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRLAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> dre:393934 MGC55886, Ube2d2, zgc:77149; zgc:55886 (EC:6.3.2.19);
K06689 ubiquitin-conjugating enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 125 bits (314), Expect = 2e-28, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F T+IYHPNI++ G
Sbjct 23 AGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL I VLLS+ +LL P PDDPL +A +K DR+ AR+W
Sbjct 83 SICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> xla:403384 ube2d3, ube2d2, ube2d3.1, xubc4; ubiquitin-conjugating
enzyme E2D 3 (UBC4/5 homolog) (EC:6.3.2.19); K06689 ubiquitin-conjugating
enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 125 bits (314), Expect = 2e-28, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F T+IYHPNI++ G
Sbjct 23 AGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL I VLLS+ +LL P PDDPL +A +K DR+ AR+W
Sbjct 83 SICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> mmu:56550 Ube2d2, 1500034D03Rik, Ubc2e, ubc4; ubiquitin-conjugating
enzyme E2D 2 (EC:6.3.2.19); K06689 ubiquitin-conjugating
enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 125 bits (314), Expect = 2e-28, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F T+IYHPNI++ G
Sbjct 23 AGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL I VLLS+ +LL P PDDPL +A +K DR+ AR+W
Sbjct 83 SICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
> hsa:7322 UBE2D2, E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH5B; ubiquitin-conjugating
enzyme E2D 2 (UBC4/5 homolog, yeast) (EC:6.3.2.19);
K06689 ubiquitin-conjugating enzyme E2 D/E [EC:6.3.2.19]
Length=147
Score = 125 bits (314), Expect = 2e-28, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 80/124 (64%), Gaps = 0/124 (0%)
Query 113 AAPDPSNYRYFKIIMTGPAGTPYEGGRYRLELFLPEQYPMEPPKVRFLTKIYHPNIDALG 172
A P + +++ + GP +PY+GG + L + P YP +PPKV F T+IYHPNI++ G
Sbjct 23 AGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNG 82
Query 173 RICLDILKDKWSPALQIRTVLLSVQALLSAPEPDDPLDTRVADHFKNDRQAAEERARQWR 232
ICLDIL+ +WSPAL I VLLS+ +LL P PDDPL +A +K DR+ AR+W
Sbjct 83 SICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWT 142
Query 233 EMYA 236
+ YA
Sbjct 143 QKYA 146
Lambda K H
0.318 0.132 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 8572894952
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40