bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_2283_orf1
Length=172
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_064130 hypothetical protein ; K13106 pre-mRNA-splic... 160 2e-39
bbo:BBOV_I000570 16.m00731; hypothetical protein; K13106 pre-m... 135 6e-32
tpv:TP02_0881 hypothetical protein; K13106 pre-mRNA-splicing f... 100 3e-21
pfa:PFL2235w conserved Plasmodium protein; K13106 pre-mRNA-spl... 89.7 4e-18
cpv:cgd4_1090 hypothetical protein 84.0 2e-16
ath:AT1G31870 hypothetical protein; K13106 pre-mRNA-splicing f... 72.4 7e-13
hsa:84811 BUD13, FLJ27090, MGC13125, fSAP71; BUD13 homolog (S.... 69.7 5e-12
mmu:215051 Bud13, AV305342, D030060M11Rik, MGC38070; BUD13 hom... 64.7 1e-10
dre:564510 fi47e11, wu:fi47e11; si:dkeyp-69c1.6; K13106 pre-mR... 62.8 5e-10
cel:R08D7.1 hypothetical protein; K13106 pre-mRNA-splicing fac... 60.5 3e-09
sce:YGL174W BUD13, CWC26; Bud13p; K13106 pre-mRNA-splicing fac... 45.8 7e-05
dre:550387 zgc:111997 35.8 0.076
sce:YHR154W RTT107, ESC4; Protein implicated in Mms22-dependen... 31.6 1.4
pfa:PF07_0016 conserved Plasmodium membrane protein, unknown f... 30.0 3.8
mmu:19419 Rasgrp1, Rasgrp; RAS guanyl releasing protein 1; K04... 30.0 4.4
> tgo:TGME49_064130 hypothetical protein ; K13106 pre-mRNA-splicing
factor CWC26
Length=673
Score = 160 bits (404), Expect = 2e-39, Method: Compositional matrix adjust.
Identities = 87/161 (54%), Positives = 112/161 (69%), Gaps = 15/161 (9%)
Query 16 VIFRDREGRIISEEEWLTLEG-AKGRKRQRERGPAPVLEWGAGLKQKEDLEARKKEEEKI 74
V++RDR+GRII+EEEWL L+ +G++R++ER P P LEWG GL QKE E + +E+ K+
Sbjct 506 VVYRDRQGRIITEEEWLELQDQKRGKRRKKERSPPPELEWGKGLVQKEAREKQAQEDAKV 565
Query 75 AKQPFARYDIDEEHDAELRARDRWDDPLAKAP-----SNTAQGLGRKQKGKEDVDREKKH 129
A+QP RYDIDEE+D L+ R RW+DP+ + P S + + KGKE
Sbjct 566 AQQPLTRYDIDEEYDRNLQDRARWEDPMNQVPQKADESRATPAVPEQSKGKE-------- 617
Query 130 QRPKCPHDAPPNRFGIKPGYRWDGVVRGNGYEEERLKALCK 170
PKCPHDAP NRFGI PGYRWDGVVRGNGYE+ RLKA+ +
Sbjct 618 -HPKCPHDAPRNRFGILPGYRWDGVVRGNGYEDRRLKAINR 657
> bbo:BBOV_I000570 16.m00731; hypothetical protein; K13106 pre-mRNA-splicing
factor CWC26
Length=254
Score = 135 bits (340), Expect = 6e-32, Method: Compositional matrix adjust.
Identities = 70/157 (44%), Positives = 94/157 (59%), Gaps = 15/157 (9%)
Query 16 VIFRDREGRIISEEEWLTLEGAKGRKRQRERGPAPVLEWGAGLKQKEDLEARKKEEEKIA 75
V++RD+ G+ I+ ++WL L + K +R PA L WG GL QK DLEA+ +EE KI+
Sbjct 99 VVYRDKSGKRITRDQWLVLHAKRVVKAKRAE-PAQELAWGKGLVQKADLEAQAQEELKIS 157
Query 76 KQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDVDREKKHQRPKCP 135
KQP RYDIDE++D +L+ R RW DP+ S T + ++ PKC
Sbjct 158 KQPLNRYDIDEDYDQQLKERTRWSDPINDTSSVT--------------NEDQPDNVPKCR 203
Query 136 HDAPPNRFGIKPGYRWDGVVRGNGYEEERLKALCKNS 172
PNRF I+PGYRWDGV+RGNGYEE KA + +
Sbjct 204 FTGLPNRFNIQPGYRWDGVIRGNGYEERWFKARARTA 240
> tpv:TP02_0881 hypothetical protein; K13106 pre-mRNA-splicing
factor CWC26
Length=227
Score = 100 bits (248), Expect = 3e-21, Method: Compositional matrix adjust.
Identities = 57/152 (37%), Positives = 86/152 (56%), Gaps = 22/152 (14%)
Query 16 VIFRDREGRIISEEEWLTLEGAKGRKRQRERGPAPVLEWGAGLKQKEDLEARKKEEEKIA 75
+++RD+ G+ I+ ++WL L + R R+ P L WG GL Q ++ E KEE+KI
Sbjct 79 IVYRDKFGKRITRDQWLLLNHREKRGREE---PKQELVWGKGLVQIKEKEEILKEEQKII 135
Query 76 KQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDVDREKKHQRPKCP 135
Q F +DID+++D EL+ + RW DP+ +N+ Q + + P+C
Sbjct 136 NQSFQNFDIDQDYDEELKEKKRWSDPIN---TNSNQ----------------QSEEPRCR 176
Query 136 HDAPPNRFGIKPGYRWDGVVRGNGYEEERLKA 167
PNRF I+PGYRWDGV+RGN YE+ +A
Sbjct 177 FSMVPNRFNIEPGYRWDGVIRGNNYEQRWFEA 208
> pfa:PFL2235w conserved Plasmodium protein; K13106 pre-mRNA-splicing
factor CWC26
Length=390
Score = 89.7 bits (221), Expect = 4e-18, Method: Compositional matrix adjust.
Identities = 58/163 (35%), Positives = 90/163 (55%), Gaps = 24/163 (14%)
Query 15 RVIFRDREGRIISEEEWL---------TLEGAKGRKRQRERGPAPVLEWGAGLKQKEDLE 65
+ I+RD+ G+IIS E+W+ K +K+++E LEW GL QK+ +
Sbjct 223 KTIYRDKSGKIISREKWIEEQKKEMKYKHGKYKDKKKKQEEEKKRKLEWDGGLVQKDIRQ 282
Query 66 ARKKEEEK-IAKQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDVD 124
+E EK I K+ Y+ D ++D EL+ + R DDP+ L K++ +E+
Sbjct 283 KNIEENEKLIKKKNIINYEFDSDYDIELKMKKRKDDPMNIY-------LEAKEENQEN-- 333
Query 125 REKKHQRPKCPHDAPPNRFGIKPGYRWDGVVRGNGYEEERLKA 167
++ C + +P NRF I+ GYRWDGV+RGNG+EE RL+A
Sbjct 334 -----KKLTCRYQSPYNRFNIQAGYRWDGVIRGNGFEERRLEA 371
> cpv:cgd4_1090 hypothetical protein
Length=177
Score = 84.0 bits (206), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 60/167 (35%), Positives = 85/167 (50%), Gaps = 25/167 (14%)
Query 16 VIFRDREGRIISEEEWL---------TLEGAKGRKRQRERGPAPVLEWGAGLKQKEDLEA 66
V++RD EGRII +E W + + + + + + P V ++ G+ Q E EA
Sbjct 5 VVYRDEEGRIIDKELWYEKKINEKRKSKKRSIKNENKFTKDPEFV-KYSGGIIQYE--EA 61
Query 67 RKK---EEEKIAKQPFA---RYDIDEEHDAELRARDRWDDP--LAKAPSNTAQGLGRKQK 118
+KK E + + RY + + +D EL+ +D WDDP L A N G +
Sbjct 62 KKKYAGENNPVIQDEIIDGGRYYLSKSYDDELKKKDIWDDPIKLINAQDNNINNSGLRS- 120
Query 119 GKEDVDREKKHQRPKCPHDAPPNRFGIKPGYRWDGVVRGNGYEEERL 165
+ VD E K R KC P NRF I+ GYRWDGV+RGNG+EE L
Sbjct 121 --DFVDLEAK--RLKCKFITPQNRFSIESGYRWDGVIRGNGFEENYL 163
> ath:AT1G31870 hypothetical protein; K13106 pre-mRNA-splicing
factor CWC26
Length=561
Score = 72.4 bits (176), Expect = 7e-13, Method: Compositional matrix adjust.
Identities = 60/169 (35%), Positives = 84/169 (49%), Gaps = 30/169 (17%)
Query 16 VIFRDR-EGRIISEEEWLTLEGAKGRKRQRERGPAPVLEWGAGLKQKEDLEARKKEEEKI 74
+FRD+ G+ IS+EE+L + K ++ +E LEWG GL QK + EAR +E E
Sbjct 385 AVFRDKITGKRISKEEYLKSKQKKVIEKPKEIK----LEWGKGLAQKREAEARLQELELE 440
Query 75 AKQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDV-----DREKKH 129
+PFAR D E D ++ R R+ DP+A L +K+K + + D E K
Sbjct 441 KDKPFARTRDDPELDQMMKERVRFGDPMA--------HLVKKRKYETTLVDLGDDEEMKK 492
Query 130 QRPKCPHDAP------------PNRFGIKPGYRWDGVVRGNGYEEERLK 166
P P NR+GIKPG WDGV R NG E++ +K
Sbjct 493 SGFIIPQSVPKHSWLTRRLEAASNRYGIKPGRHWDGVDRSNGTEKDLIK 541
> hsa:84811 BUD13, FLJ27090, MGC13125, fSAP71; BUD13 homolog (S.
cerevisiae); K13106 pre-mRNA-splicing factor CWC26
Length=485
Score = 69.7 bits (169), Expect = 5e-12, Method: Compositional matrix adjust.
Identities = 56/164 (34%), Positives = 81/164 (49%), Gaps = 18/164 (10%)
Query 9 GEIPASRVIFRDREGRIISEEEWLTLEGAKGRKRQRERGPAPVL--EWGAGLKQKEDLEA 66
E + +FRD+ GR + L LE + R++ + L +WG GL Q +
Sbjct 320 AEFQYAETVFRDKSGR----KRNLKLERLEQRRKAEKDSERDELYAQWGKGLAQSRQQQQ 375
Query 67 RKKEEEKIAKQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDVDRE 126
++ K ++P ARY DE+ D LR ++R DP+A N + K+ + V
Sbjct 376 NVEDAMKEMQKPLARYIDDEDLDRMLREQEREGDPMA----NFIKKNKAKENKNKKV--- 428
Query 127 KKHQRPKCPHDAPP-NRFGIKPGYRWDGVVRGNGYEEERLKALC 169
RP+ APP NRF I PGYRWDGV R NG+E++R L
Sbjct 429 ----RPRYSGPAPPPNRFNIWPGYRWDGVDRSNGFEQKRFARLA 468
> mmu:215051 Bud13, AV305342, D030060M11Rik, MGC38070; BUD13 homolog
(yeast); K13106 pre-mRNA-splicing factor CWC26
Length=637
Score = 64.7 bits (156), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 51/156 (32%), Positives = 76/156 (48%), Gaps = 16/156 (10%)
Query 16 VIFRDREGRIISEEEWLTLEGAKGRKRQRERGPAPVL--EWGAGLKQKEDLEARKKEEEK 73
+FRD+ GR + L LE + R++ + L +WG GL Q + ++ K
Sbjct 479 TVFRDKSGR----KRNLKLERLEQRRKAEKDSERDELYAQWGKGLAQSRQQQQNVEDAMK 534
Query 74 IAKQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDVDREKKHQRPK 133
++P ARY DE+ D LR ++R DP+A + + + + + K
Sbjct 535 EMQKPLARYIDDEDLDRMLREQEREGDPMANF-------IKKNKAKENKNKKVKPRYSGP 587
Query 134 CPHDAPPNRFGIKPGYRWDGVVRGNGYEEERLKALC 169
P PPNRF I PGYRWDGV R NG+E++R L
Sbjct 588 AP---PPNRFNIWPGYRWDGVDRSNGFEQKRFARLA 620
> dre:564510 fi47e11, wu:fi47e11; si:dkeyp-69c1.6; K13106 pre-mRNA-splicing
factor CWC26
Length=518
Score = 62.8 bits (151), Expect = 5e-10, Method: Compositional matrix adjust.
Identities = 54/159 (33%), Positives = 82/159 (51%), Gaps = 16/159 (10%)
Query 16 VIFRDREGR---IISEEEWLTLEGAKGRKRQRERGPAPVLEWGAGLKQKEDLEARKKEEE 72
IFRD+ GR + SE+ L+ + ++ ++ + +WG GL Q E E +
Sbjct 358 TIFRDKSGRKRNLESEKAELSQKASEKAEKDEK-----YAQWGKGLAQGEMQEQNVIDAI 412
Query 73 KIAKQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDVDREKKHQRP 132
+ A++P AR+ DE+ D LR ++R DP+A + RK+K K + K +
Sbjct 413 REAEKPLARHIDDEDLDRMLREQEREGDPMA--------AMLRKKKDKTAKLKGVKEKPC 464
Query 133 KCPHDAPPNRFGIKPGYRWDGVVRGNGYEEERLKALCKN 171
PPNRF I PGYRWDGV R NG+E++R + N
Sbjct 465 YKGPPPPPNRFNIMPGYRWDGVDRSNGFEQKRYSRMADN 503
> cel:R08D7.1 hypothetical protein; K13106 pre-mRNA-splicing factor
CWC26
Length=458
Score = 60.5 bits (145), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 39/108 (36%), Positives = 56/108 (51%), Gaps = 9/108 (8%)
Query 54 WGAGLKQKEDLEARKKEEEKIAKQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGL 113
W G+ Q ED A+ +E ++A +P AR D+ +A L+ DP+A +
Sbjct 335 WNKGVAQIEDRRAQLEEMARVAAEPMARARDDDAMNAHLKEVLHAADPMA--------NM 386
Query 114 GRKQKGKEDVDREKKHQRPKCPHDAPPNRFGIKPGYRWDGVVRGNGYE 161
+K++ +DR + P PNRFGI PGYRWDGV R NG+E
Sbjct 387 IQKKRRDTAIDR-GELVYPSYHGHFVPNRFGIAPGYRWDGVDRSNGFE 433
> sce:YGL174W BUD13, CWC26; Bud13p; K13106 pre-mRNA-splicing factor
CWC26
Length=266
Score = 45.8 bits (107), Expect = 7e-05, Method: Compositional matrix adjust.
Identities = 18/25 (72%), Positives = 19/25 (76%), Gaps = 0/25 (0%)
Query 138 APPNRFGIKPGYRWDGVVRGNGYEE 162
AP NRF I PG RWDGV R NG+EE
Sbjct 219 APENRFAIMPGSRWDGVHRSNGFEE 243
> dre:550387 zgc:111997
Length=345
Score = 35.8 bits (81), Expect = 0.076, Method: Compositional matrix adjust.
Identities = 30/108 (27%), Positives = 48/108 (44%), Gaps = 14/108 (12%)
Query 16 VIFRDREGRIISEEEWLTLEGAK--GRKRQRERGPAPV-----------LEWGAGLKQKE 62
V F R+I + EW+ EGA R+ QRE G PV AG++ +
Sbjct 49 VYFDSNIKRMIPKTEWIKNEGADYWDRESQRESGIVPVFKNNIQIAMQRFNQTAGVRTVQ 108
Query 63 DLEARKKEEEKIAKQPFARYDIDEEHDAELRARD-RWDDPLAKAPSNT 109
++ + +++ AK F +Y D E L ++ RW P+ +A T
Sbjct 109 NMYGCEWDDQTGAKNGFDQYGYDGEDWLTLELKELRWISPVQQAMITT 156
> sce:YHR154W RTT107, ESC4; Protein implicated in Mms22-dependent
DNA repair during S phase, DNA damage induces phosphorylation
by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p
and Slx4p; has four BRCT domains; has a role in regulation
of Ty1 transposition
Length=1070
Score = 31.6 bits (70), Expect = 1.4, Method: Composition-based stats.
Identities = 17/69 (24%), Positives = 32/69 (46%), Gaps = 0/69 (0%)
Query 65 EARKKEEEKIAKQPFARYDIDEEHDAELRARDRWDDPLAKAPSNTAQGLGRKQKGKEDVD 124
E +K EEK +P YD ++ + ++R + D K T + Q+ K ++
Sbjct 604 EGHEKREEKEFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREIK 663
Query 125 REKKHQRPK 133
KK+++ K
Sbjct 664 TSKKNEKEK 672
> pfa:PF07_0016 conserved Plasmodium membrane protein, unknown
function
Length=1859
Score = 30.0 bits (66), Expect = 3.8, Method: Composition-based stats.
Identities = 18/67 (26%), Positives = 34/67 (50%), Gaps = 5/67 (7%)
Query 69 KEEEKIAKQPFARYDIDEEHDAELR--ARDRWDD---PLAKAPSNTAQGLGRKQKGKEDV 123
KE+ KI K+P Y I+E +++ A+ D P+ T + LG+ Q G +D+
Sbjct 1737 KEQNKIIKEPLEEYKINEHEKNDIKDSAKVHKTDIHTPVLNNEVETQEMLGKSQVGNQDI 1796
Query 124 DREKKHQ 130
+ + ++
Sbjct 1797 NTFRNNE 1803
> mmu:19419 Rasgrp1, Rasgrp; RAS guanyl releasing protein 1; K04350
RAS guanyl-releasing protein 1
Length=795
Score = 30.0 bits (66), Expect = 4.4, Method: Composition-based stats.
Identities = 11/40 (27%), Positives = 22/40 (55%), Gaps = 0/40 (0%)
Query 48 PAPVLEWGAGLKQKEDLEARKKEEEKIAKQPFARYDIDEE 87
P V++W +G+ K D + K +++ F YD+D++
Sbjct 448 PPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQD 487
Lambda K H
0.313 0.135 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 4341553636
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40