bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_3229_orf1
Length=141
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_118560 3-methyl-2-oxobutanoate dehydrogenase (lipoa... 188 6e-48
mmu:12041 Bckdk, AI327402; branched chain ketoacid dehydrogena... 76.6 2e-14
hsa:10295 BCKDK; branched chain ketoacid dehydrogenase kinase ... 76.3 3e-14
ath:AT3G06483 PDK; PDK (PYRUVATE DEHYDROGENASE KINASE); ATP bi... 75.9 3e-14
tgo:TGME49_019680 hypothetical protein 75.9 4e-14
xla:432076 bckdk, MGC78818; branched chain ketoacid dehydrogen... 74.7 9e-14
dre:282677 bckdk, cb233, wu:fa04g07, wu:fb80b08, zgc:55984; br... 68.9 5e-12
hsa:5165 PDK3; pyruvate dehydrogenase kinase, isozyme 3 (EC:2.... 60.5 2e-09
mmu:236900 Pdk3, 2610001M10Rik, AI035637, MGC6383; pyruvate de... 58.2 8e-09
dre:794083 pyruvate dehydrogenase kinase 2-like 57.8 1e-08
dre:555840 pyruvate dehydrogenase kinase 1-like; K12077 pyruva... 56.2 3e-08
hsa:5166 PDK4, FLJ40832; pyruvate dehydrogenase kinase, isozym... 53.1 2e-07
dre:560068 pdk3a, pdk3; pyruvate dehydrogenase kinase, isozyme... 53.1 3e-07
mmu:27273 Pdk4, AV005916; pyruvate dehydrogenase kinase, isoen... 52.8 3e-07
mmu:18604 Pdk2; pyruvate dehydrogenase kinase, isoenzyme 2 (EC... 52.0 5e-07
dre:561007 si:rp71-57j15.4 (EC:2.7.11.2); K00898 pyruvate dehy... 52.0 6e-07
hsa:5164 PDK2, PDHK2; pyruvate dehydrogenase kinase, isozyme 2... 52.0 7e-07
xla:398840 hypothetical protein MGC68579; K00898 pyruvate dehy... 50.8 1e-06
xla:494745 hypothetical LOC494745; K12077 pyruvate dehydrogena... 50.8 1e-06
mmu:228026 Pdk1, B830012B01, D530020C15Rik; pyruvate dehydroge... 50.4 2e-06
xla:432080 pdk1, MGC81400; pyruvate dehydrogenase kinase, isoz... 50.4 2e-06
cel:ZK370.5 pdhk-2; Pyruvate DeHydogenase Kinase family member... 49.7 3e-06
dre:393971 pdk2, MGC56209, Pdk, zgc:56209; pyruvate dehydrogen... 47.4 1e-05
hsa:5163 PDK1; pyruvate dehydrogenase kinase, isozyme 1 (EC:2.... 47.0 2e-05
sce:YIL042C PKP1; Mitochondrial protein kinase involved in neg... 45.4 6e-05
xla:379789 pdk4, 3j828, MGC132353, MGC52849; pyruvate dehydrog... 44.7 9e-05
sce:YGL059W PKP2; Mitochondrial protein kinase that negatively... 33.1 0.28
hsa:100288524 forkhead box L1-like 30.0 2.7
mmu:384244 Gm5294, EG384244; predicted gene 5294 29.6
ath:AT4G23620 50S ribosomal protein-related 29.3 4.4
> tgo:TGME49_118560 3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
kinase, putative (EC:2.7.11.4); K00905 [3-methyl-2-oxobutanoate
dehydrogenase (acetyl-transferring)] kinase [EC:2.7.11.4]
Length=432
Score = 188 bits (477), Expect = 6e-48, Method: Compositional matrix adjust.
Identities = 82/141 (58%), Positives = 117/141 (82%), Gaps = 0/141 (0%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLDVSFDFFLERFFYFRIGRRVMVNNLLAMQ 60
FV+L+E+ER HD +VDL+G+ ++QL+R+ DV + FLERFFYFRIGRRVM++ L+ +Q
Sbjct 148 FVQLVEKERSTHDRTVDLMGQGVRQLRRICRDVDLNSFLERFFYFRIGRRVMIDQLVHLQ 207
Query 61 NPKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVAGNLETEFATIPEHLAL 120
+ + GW GI++ C A++I R+++VR+SC+ SYG+AP VV++GN + +FATIP+HLAL
Sbjct 208 SKQEGWQGIIHLNCHAAKIIEQRSKDVRESCRHSYGLAPRVVISGNTDMKFATIPDHLAL 267
Query 121 IITEVLKNALRATVEFYTLGN 141
I+TEVLKNALRATVEF+T+GN
Sbjct 268 IVTEVLKNALRATVEFHTMGN 288
> mmu:12041 Bckdk, AI327402; branched chain ketoacid dehydrogenase
kinase (EC:2.7.11.4); K00905 [3-methyl-2-oxobutanoate dehydrogenase
(acetyl-transferring)] kinase [EC:2.7.11.4]
Length=412
Score = 76.6 bits (187), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 44/138 (31%), Positives = 72/138 (52%), Gaps = 1/138 (0%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLDVSF-DFFLERFFYFRIGRRVMVNNLLAM 59
+ +L+ Q H V LL L++ ++ D +FL++ R+G R++ + LA+
Sbjct 151 YCQLVRQLLDDHKDVVTLLAEGLRESRKHIQDEKLVRYFLDKTLTSRLGIRMLATHHLAL 210
Query 60 QNPKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVAGNLETEFATIPEHLA 119
KP + GI+ R P ++I R C+ YG AP V + G++ F IP L
Sbjct 211 HEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLD 270
Query 120 LIITEVLKNALRATVEFY 137
I+ E+LKNA+RAT+E +
Sbjct 271 YILPELLKNAMRATMESH 288
> hsa:10295 BCKDK; branched chain ketoacid dehydrogenase kinase
(EC:2.7.11.4); K00905 [3-methyl-2-oxobutanoate dehydrogenase
(acetyl-transferring)] kinase [EC:2.7.11.4]
Length=365
Score = 76.3 bits (186), Expect = 3e-14, Method: Compositional matrix adjust.
Identities = 44/138 (31%), Positives = 72/138 (52%), Gaps = 1/138 (0%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLDVSF-DFFLERFFYFRIGRRVMVNNLLAM 59
+ +L+ Q H V LL L++ ++ D +FL++ R+G R++ + LA+
Sbjct 151 YCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLAL 210
Query 60 QNPKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVAGNLETEFATIPEHLA 119
KP + GI+ R P ++I R C+ YG AP V + G++ F IP L
Sbjct 211 HEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLD 270
Query 120 LIITEVLKNALRATVEFY 137
I+ E+LKNA+RAT+E +
Sbjct 271 YILPELLKNAMRATMESH 288
> ath:AT3G06483 PDK; PDK (PYRUVATE DEHYDROGENASE KINASE); ATP
binding / histidine phosphotransfer kinase/ pyruvate dehydrogenase
(acetyl-transferring) kinase
Length=366
Score = 75.9 bits (185), Expect = 3e-14, Method: Compositional matrix adjust.
Identities = 41/140 (29%), Positives = 76/140 (54%), Gaps = 5/140 (3%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLDVSFDF---FLERFFYFRIGRRVMVNNLL 57
F ++++ + +H++ V ++ + QLK+ + D FL+RF+ RIG R+++ +
Sbjct 110 FTQMIKAVKVRHNNVVPMMALGVNQLKKGMNSGNLDEIHQFLDRFYLSRIGIRMLIGQHV 169
Query 58 AMQNPKPGW--CGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVAGNLETEFATIP 115
+ NP P G ++ + P EV +++ R C YG AP + + G+ F +P
Sbjct 170 ELHNPNPPLHTVGYIHTKMSPMEVARNASEDARSICFREYGSAPEINIYGDPSFTFPYVP 229
Query 116 EHLALIITEVLKNALRATVE 135
HL L++ E++KN+LRA E
Sbjct 230 THLHLMMYELVKNSLRAVQE 249
> tgo:TGME49_019680 hypothetical protein
Length=808
Score = 75.9 bits (185), Expect = 4e-14, Method: Compositional matrix adjust.
Identities = 48/141 (34%), Positives = 73/141 (51%), Gaps = 11/141 (7%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKR----LSLDVSFDFFLERFFYFRIGRRVMVNNL 56
F KLL+ +++H LL L+ LK+ + D D FL+ FF RIG ++ +
Sbjct 349 FRKLLKDLKRRHAPIAPLLVTGLRNLKKRFPDIFTDEFVDDFLDGFFLSRIGTEMLTSAY 408
Query 57 LAMQNPKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVAGNLETEFATIPE 116
L+ GIV+ C P +VI A + C + YG P V++ N FA +P+
Sbjct 409 LSP-------SGIVDTDCDPMQVIKKAAGDAEKLCHYHYGCCPRVLIWNNERERFACVPQ 461
Query 117 HLALIITEVLKNALRATVEFY 137
+L I+ E+ KNA+RATVE +
Sbjct 462 YLYYILFELFKNAMRATVERF 482
> xla:432076 bckdk, MGC78818; branched chain ketoacid dehydrogenase
kinase (EC:2.7.11.4); K00905 [3-methyl-2-oxobutanoate
dehydrogenase (acetyl-transferring)] kinase [EC:2.7.11.4]
Length=413
Score = 74.7 bits (182), Expect = 9e-14, Method: Compositional matrix adjust.
Identities = 43/138 (31%), Positives = 71/138 (51%), Gaps = 1/138 (0%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLDVS-FDFFLERFFYFRIGRRVMVNNLLAM 59
+ KLL Q H V L L++ ++ D +FL++ R+G R++ + LA+
Sbjct 151 YCKLLRQLLDDHKDVVTQLAEGLRESRKHIQDEKVISYFLDKTLTSRLGIRMLATHHLAL 210
Query 60 QNPKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVAGNLETEFATIPEHLA 119
+P + GI+ R P ++I R C+ YG AP V + G++ F IP L
Sbjct 211 HEERPDFVGIICTRLWPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLD 270
Query 120 LIITEVLKNALRATVEFY 137
I+ E+LKN++RAT+E +
Sbjct 271 YILPELLKNSMRATMESH 288
> dre:282677 bckdk, cb233, wu:fa04g07, wu:fb80b08, zgc:55984;
branched chain alpha-ketoacid dehydrogenase kinase (EC:2.7.11.4);
K00905 [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)]
kinase [EC:2.7.11.4]
Length=419
Score = 68.9 bits (167), Expect = 5e-12, Method: Compositional matrix adjust.
Identities = 40/138 (28%), Positives = 71/138 (51%), Gaps = 1/138 (0%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLD-VSFDFFLERFFYFRIGRRVMVNNLLAM 59
+ K+++Q H V +L ++ ++ LD + FL+ R+G R++ + +A+
Sbjct 159 YSKMVKQLLDDHKDVVTMLAEGFRECRKHILDEMLVRNFLDTTLTSRLGIRMLATHHIAL 218
Query 60 QNPKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVAGNLETEFATIPEHLA 119
P + GI+ R P ++I R C+ YG +P V + G++ F IP L
Sbjct 219 HEDNPDFVGIICRRLSPKKIIEKWVDFARRLCEHQYGNSPRVRINGHVAARFPFIPLPLD 278
Query 120 LIITEVLKNALRATVEFY 137
I+ E+LKNA+RAT+E +
Sbjct 279 YILPELLKNAMRATMESH 296
> hsa:5165 PDK3; pyruvate dehydrogenase kinase, isozyme 3 (EC:2.7.11.2);
K00898 pyruvate dehydrogenase kinase [EC:2.7.11.2]
Length=415
Score = 60.5 bits (145), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 41/153 (26%), Positives = 77/153 (50%), Gaps = 16/153 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLK-RLSLD----VSFDFFLERFFYFRIGRRVMVNN 55
F+++L + R +H+ V + + + + K + D + +FL+RF+ RI R+++N
Sbjct 108 FLQVLIKVRNRHNDVVPTMAQGVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQ 167
Query 56 LLAM----QNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVV------A 104
+ NP P G ++P C A+V+ + + C+ Y +AP + V A
Sbjct 168 HTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKA 227
Query 105 GNLETEFATIPEHLALIITEVLKNALRATVEFY 137
+ + +P HL ++ E+ KN++RATVE Y
Sbjct 228 PDKPIQVVYVPSHLFHMLFELFKNSMRATVELY 260
> mmu:236900 Pdk3, 2610001M10Rik, AI035637, MGC6383; pyruvate
dehydrogenase kinase, isoenzyme 3 (EC:2.7.11.2); K00898 pyruvate
dehydrogenase kinase [EC:2.7.11.2]
Length=415
Score = 58.2 bits (139), Expect = 8e-09, Method: Compositional matrix adjust.
Identities = 41/153 (26%), Positives = 75/153 (49%), Gaps = 16/153 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLK-RLSLD----VSFDFFLERFFYFRIGRRVMVNN 55
F+ +L R +H+ V + + + + K + D + +FL+RF+ RI R+++N
Sbjct 108 FLNVLINIRNRHNDVVPTMAQGVIEYKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQ 167
Query 56 LLAM----QNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVV------A 104
+ NP P G ++P C A+V+ + + C+ Y +AP + V A
Sbjct 168 HTLLFGGDTNPAHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKA 227
Query 105 GNLETEFATIPEHLALIITEVLKNALRATVEFY 137
N + +P HL ++ E+ KN++RATVE +
Sbjct 228 PNKPIQVVYVPSHLFHMLFELFKNSMRATVELH 260
> dre:794083 pyruvate dehydrogenase kinase 2-like
Length=409
Score = 57.8 bits (138), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 42/157 (26%), Positives = 71/157 (45%), Gaps = 16/157 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRL-----SLDVSFDFFLERFFYFRIGRRVMVNN 55
FV L R +H+ V + + + + K + + + +FL+RF+ RI R+++N
Sbjct 112 FVAALVNIRNRHNDVVPTMAQGVIEYKEVFGQDPVTNQNIQYFLDRFYLSRISIRMLINQ 171
Query 56 ----LLAMQNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAP------LVVVA 104
NP P G ++P C+ A+V+ + R C Y +P L
Sbjct 172 HTLVFDGATNPVHPNTIGSIDPHCQVADVVKDAYESARMLCDQYYLNSPDLDLQELNTNN 231
Query 105 GNLETEFATIPEHLALIITEVLKNALRATVEFYTLGN 141
N +P HL I+ E+ KNA+RAT+E + G+
Sbjct 232 RNQPISIVYVPSHLYHILFELFKNAMRATIENHKEGS 268
> dre:555840 pyruvate dehydrogenase kinase 1-like; K12077 pyruvate
dehydrogenase kinase isoform 1 [EC:2.7.11.2]
Length=405
Score = 56.2 bits (134), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 33/114 (28%), Positives = 56/114 (49%), Gaps = 13/114 (11%)
Query 37 FFLERFFYFRIGRRVMVNNLLAM------QNP-KPGWCGIVNPRCRPAEVIMARAQEVRD 89
+FL+RF+ RI R+++N + NP P G ++P CR +V+ + R+
Sbjct 148 YFLDRFYMSRISIRMLLNQHTLLFGGKVRDNPAHPKQIGSIDPSCRVTDVVKDAYENARN 207
Query 90 SCKFSYGIAPLVVV------AGNLETEFATIPEHLALIITEVLKNALRATVEFY 137
C Y +P +V+ + +P HL ++ E+ KNA+RAT+E Y
Sbjct 208 LCDRYYMNSPELVLEEFNVKGPDKPVTVVYVPSHLYHMVFELFKNAMRATMELY 261
> hsa:5166 PDK4, FLJ40832; pyruvate dehydrogenase kinase, isozyme
4 (EC:2.7.11.2); K00898 pyruvate dehydrogenase kinase [EC:2.7.11.2]
Length=411
Score = 53.1 bits (126), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 40/150 (26%), Positives = 68/150 (45%), Gaps = 15/150 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSL-----DVSFDFFLERFFYFRIGRRVMVNN 55
FV L + R +H + V + + + + K + + +FL+RF+ RI R+++N
Sbjct 116 FVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQ 175
Query 56 LLAM----QNPKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAP---LVVVAGNLE 108
+ + Q P G ++P C V+ + R C Y +P L V G
Sbjct 176 HILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFP 235
Query 109 TE---FATIPEHLALIITEVLKNALRATVE 135
+ +P HL ++ E+ KNA+RATVE
Sbjct 236 DQPIHIVYVPSHLHHMLFELFKNAMRATVE 265
> dre:560068 pdk3a, pdk3; pyruvate dehydrogenase kinase, isozyme
3a; K00898 pyruvate dehydrogenase kinase [EC:2.7.11.2]
Length=404
Score = 53.1 bits (126), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 39/153 (25%), Positives = 73/153 (47%), Gaps = 16/153 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLK-RLSLD----VSFDFFLERFFYFRIGRRVMVNN 55
F++ L + R +H+ V + + + + K + D + +FL+RF+ RI R+++N
Sbjct 107 FLETLIEIRNRHNDVVPTMAQGVIEYKEKFGFDPFVSSNIQYFLDRFYTNRISFRMLINQ 166
Query 56 LLAM----QNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVV------A 104
+ NP P G ++P C AEV+ + + C+ Y AP + + A
Sbjct 167 HTLLFGNDINPAHPKHIGSIDPSCNVAEVVTDAYETAKMVCEQYYQAAPELKIEEFNAKA 226
Query 105 GNLETEFATIPEHLALIITEVLKNALRATVEFY 137
+ +P HL ++ E+ KNA+RAT + +
Sbjct 227 PQKPIQAVYVPSHLFHMLFELFKNAMRATNDLH 259
> mmu:27273 Pdk4, AV005916; pyruvate dehydrogenase kinase, isoenzyme
4 (EC:2.7.11.2); K00898 pyruvate dehydrogenase kinase
[EC:2.7.11.2]
Length=412
Score = 52.8 bits (125), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 40/150 (26%), Positives = 70/150 (46%), Gaps = 15/150 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSL-----DVSFDFFLERFFYFRIGRRVMVN- 54
FV L + R +H + V + + + + K + + +FL+RF+ RI R+++N
Sbjct 116 FVDTLVKVRNRHHNVVPTMAQGILEYKDTCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQ 175
Query 55 NLLAMQNPK---PGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAP---LVVVAGNLE 108
++L + K P G ++P C V+ + + C Y +P L V G
Sbjct 176 HILIFSDSKTGNPSHIGSIDPNCDVVAVVQDAFECAKMLCDQYYLTSPELNLTQVNGKFP 235
Query 109 TE---FATIPEHLALIITEVLKNALRATVE 135
+ +P HL ++ E+ KNA+RATVE
Sbjct 236 GQPIHIVYVPSHLHHMLFELFKNAMRATVE 265
> mmu:18604 Pdk2; pyruvate dehydrogenase kinase, isoenzyme 2 (EC:2.7.11.2);
K00898 pyruvate dehydrogenase kinase [EC:2.7.11.2]
Length=407
Score = 52.0 bits (123), Expect = 5e-07, Method: Compositional matrix adjust.
Identities = 38/151 (25%), Positives = 64/151 (42%), Gaps = 16/151 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLD-----VSFDFFLERFFYFRIGRRVMVNN 55
F L R +H+ V + + + + K D + +FL+RF+ RI R+++N
Sbjct 112 FTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQ 171
Query 56 ----LLAMQNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAP------LVVVA 104
NP P G ++P C ++V+ + C Y +P +
Sbjct 172 HTLIFDGSTNPAHPKHIGSIDPNCSVSDVVKDAYDMAKLLCDKYYMASPDLEIQEVNATN 231
Query 105 GNLETEFATIPEHLALIITEVLKNALRATVE 135
N +P HL ++ E+ KNA+RATVE
Sbjct 232 ANQPIHMVYVPSHLYHMLFELFKNAMRATVE 262
> dre:561007 si:rp71-57j15.4 (EC:2.7.11.2); K00898 pyruvate dehydrogenase
kinase [EC:2.7.11.2]
Length=409
Score = 52.0 bits (123), Expect = 6e-07, Method: Compositional matrix adjust.
Identities = 39/151 (25%), Positives = 68/151 (45%), Gaps = 16/151 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKR-LSLDV----SFDFFLERFFYFRIGRRVMVNN 55
F + L R +H++ V + + + + K +D + +FL+RF+ RI R+++N
Sbjct 112 FTETLINVRNRHNNVVPTMAQGVLEYKEAFGVDPVTNQNVQYFLDRFYMSRISTRMLMNQ 171
Query 56 ----LLAMQNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLV------VVA 104
NP P G ++P C EV+ + + C Y +P V +
Sbjct 172 HTLIFDGSTNPAHPKHIGSIDPNCDVVEVVRDAYESSKMLCDQYYLTSPEVEIKQVNIKG 231
Query 105 GNLETEFATIPEHLALIITEVLKNALRATVE 135
+ +P HL ++ E+ KNA+RATVE
Sbjct 232 PSDPIHIVYVPSHLYHMLFELFKNAMRATVE 262
> hsa:5164 PDK2, PDHK2; pyruvate dehydrogenase kinase, isozyme
2 (EC:2.7.11.2); K00898 pyruvate dehydrogenase kinase [EC:2.7.11.2]
Length=343
Score = 52.0 bits (123), Expect = 7e-07, Method: Compositional matrix adjust.
Identities = 40/151 (26%), Positives = 67/151 (44%), Gaps = 16/151 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLD-----VSFDFFLERFFYFRIGRRVMVNN 55
F L R +H+ V + + + + K D + +FL+RF+ RI R+++N
Sbjct 48 FTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQ 107
Query 56 ----LLAMQNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVV----AGN 106
NP P G ++P C +EV+ + C Y +P + + A N
Sbjct 108 HTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAAN 167
Query 107 LET--EFATIPEHLALIITEVLKNALRATVE 135
+ +P HL ++ E+ KNA+RATVE
Sbjct 168 SKQPIHMVYVPSHLYHMLFELFKNAMRATVE 198
> xla:398840 hypothetical protein MGC68579; K00898 pyruvate dehydrogenase
kinase [EC:2.7.11.2]
Length=404
Score = 50.8 bits (120), Expect = 1e-06, Method: Compositional matrix adjust.
Identities = 39/151 (25%), Positives = 71/151 (47%), Gaps = 16/151 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRL-----SLDVSFDFFLERFFYFRIGRRVMVNN 55
F + L R +H++ V + + + + K + + +FL+RF+ RI R+++N
Sbjct 112 FTEALVTIRNRHNNVVPTMAQGVIEYKEAFGVDPVTNQNVQYFLDRFYMSRISIRMLINQ 171
Query 56 LLAM----QNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLVVVA---GNL 107
+ NP P G ++P C EV+ + C+ Y +P + + GN
Sbjct 172 HTLLFDGGTNPAHPKHIGSIDPNCDVVEVVHDAFDTAKMLCEQYYLASPELRIKQTNGND 231
Query 108 ETE---FATIPEHLALIITEVLKNALRATVE 135
++ +P HL ++ E+ KNA+RATVE
Sbjct 232 PSQPIHIVYVPSHLYHMLFELFKNAMRATVE 262
> xla:494745 hypothetical LOC494745; K12077 pyruvate dehydrogenase
kinase isoform 1 [EC:2.7.11.2]
Length=412
Score = 50.8 bits (120), Expect = 1e-06, Method: Compositional matrix adjust.
Identities = 32/113 (28%), Positives = 56/113 (49%), Gaps = 13/113 (11%)
Query 37 FFLERFFYFRIGRRVMVNNLLAM------QNP-KPGWCGIVNPRCRPAEVIMARAQEVRD 89
+FL+RF+ RI R+++N + NP P G ++P C +V+ + +
Sbjct 155 YFLDRFYMSRISIRMLLNQHTLLFGGKVKVNPAHPKHIGSIDPTCNVVDVVKDGYENAKH 214
Query 90 SCKFSYGIAP-LVVVAGNLETE-----FATIPEHLALIITEVLKNALRATVEF 136
C Y +P L + N +++ +P HL ++ E+ KNA+RAT+EF
Sbjct 215 LCDLYYMSSPELELTEFNAKSQGQPIQVVYVPSHLYHMVFELFKNAMRATMEF 267
> mmu:228026 Pdk1, B830012B01, D530020C15Rik; pyruvate dehydrogenase
kinase, isoenzyme 1 (EC:2.7.11.2); K12077 pyruvate dehydrogenase
kinase isoform 1 [EC:2.7.11.2]
Length=434
Score = 50.4 bits (119), Expect = 2e-06, Method: Compositional matrix adjust.
Identities = 38/145 (26%), Positives = 66/145 (45%), Gaps = 16/145 (11%)
Query 9 RQQHDSSVDLLGRALQQLKR-LSLDV----SFDFFLERFFYFRIGRRVMVN--NLLAMQN 61
R +H+ + + + + + K +D + +FL+RF+ RI R+++N +LL
Sbjct 146 RNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGK 205
Query 62 PKPGW---CGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAP------LVVVAGNLETEFA 112
P G +NP C EVI + R C Y +P L + +
Sbjct 206 GSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYVNSPELELEELNAKSPGQTIQVV 265
Query 113 TIPEHLALIITEVLKNALRATVEFY 137
+P HL ++ E+ KNA+RAT+E +
Sbjct 266 YVPSHLYHMVFELFKNAMRATMEHH 290
> xla:432080 pdk1, MGC81400; pyruvate dehydrogenase kinase, isozyme
1 (EC:2.7.11.2); K12077 pyruvate dehydrogenase kinase
isoform 1 [EC:2.7.11.2]
Length=412
Score = 50.4 bits (119), Expect = 2e-06, Method: Compositional matrix adjust.
Identities = 32/113 (28%), Positives = 56/113 (49%), Gaps = 13/113 (11%)
Query 37 FFLERFFYFRIGRRVMVNNLLAM------QNP-KPGWCGIVNPRCRPAEVIMARAQEVRD 89
+FL+RF+ RI R+++N + NP P G ++P C +V+ + +
Sbjct 155 YFLDRFYMSRISIRMLLNQHTLLFGGEVKVNPAHPKHIGSIDPACNVVDVVKDGYENAKH 214
Query 90 SCKFSYGIAP-LVVVAGNLET-----EFATIPEHLALIITEVLKNALRATVEF 136
C Y +P L + N ++ + +P HL ++ E+ KNA+RAT+EF
Sbjct 215 LCDLYYMSSPELELTEFNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEF 267
> cel:ZK370.5 pdhk-2; Pyruvate DeHydogenase Kinase family member
(pdhk-2); K00898 pyruvate dehydrogenase kinase [EC:2.7.11.2]
Length=401
Score = 49.7 bits (117), Expect = 3e-06, Method: Compositional matrix adjust.
Identities = 36/147 (24%), Positives = 67/147 (45%), Gaps = 15/147 (10%)
Query 10 QQHDSSVDLLGRALQQLKR-----LSLDVSFDFFLERFFYFRIGRRVMVN-NLLAMQN-- 61
++H V+ + L +L+ ++ + +FL+RF+ RI R++ N +L+ N
Sbjct 118 KRHAHVVETMAEGLIELRESDGVDIASEKGIQYFLDRFYINRISIRMLQNQHLVVFGNVL 177
Query 62 -PKPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLV------VVAGNLETEFATI 114
P G ++P C V+ + R C Y +P + V +
Sbjct 178 PESPRHVGCIDPACDVESVVYDAFENARFLCDRYYLTSPSMKLEMHNAVEKGKPISIVAV 237
Query 115 PEHLALIITEVLKNALRATVEFYTLGN 141
P HL ++ E+ KNA+RATVE++ + +
Sbjct 238 PSHLYHMMFELFKNAMRATVEYHGVDD 264
> dre:393971 pdk2, MGC56209, Pdk, zgc:56209; pyruvate dehydrogenase
kinase, isoenzyme 2 (EC:2.7.11.2); K00898 pyruvate dehydrogenase
kinase [EC:2.7.11.2]
Length=409
Score = 47.4 bits (111), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 37/151 (24%), Positives = 70/151 (46%), Gaps = 16/151 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRL-----SLDVSFDFFLERFFYFRIGRRVMVNN 55
FV+ L R +H+ V + + + + + + +FL+RF+ R+ R+++N
Sbjct 112 FVETLVTIRNRHNDVVATMAQGVIEYRDTFGADPVTSQNIQYFLDRFYMSRVSIRMLINQ 171
Query 56 ----LLAMQNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAPLV----VVAGN 106
NP P G ++ C +VI + + C+ +Y +P + + A N
Sbjct 172 HTLIFDGSTNPGHPSSIGCIDSCCDVTDVIRDAYESAKMLCEQNYLGSPELELREINAKN 231
Query 107 LET--EFATIPEHLALIITEVLKNALRATVE 135
E + +P HL ++ E+ KNA+RAT+E
Sbjct 232 KSKPIEISYVPSHLYHMVFELFKNAMRATIE 262
> hsa:5163 PDK1; pyruvate dehydrogenase kinase, isozyme 1 (EC:2.7.11.2);
K12077 pyruvate dehydrogenase kinase isoform 1 [EC:2.7.11.2]
Length=436
Score = 47.0 bits (110), Expect = 2e-05, Method: Compositional matrix adjust.
Identities = 34/115 (29%), Positives = 53/115 (46%), Gaps = 13/115 (11%)
Query 37 FFLERFFYFRIGRRVMVN--NLLAMQNPKPG-----WCGIVNPRCRPAEVIMARAQEVRD 89
+FL+RF+ RI R+++N +LL K G +NP C EVI + R
Sbjct 179 YFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARR 238
Query 90 SCKFSYGIAP------LVVVAGNLETEFATIPEHLALIITEVLKNALRATVEFYT 138
C Y +P L + + +P HL ++ E+ KNA+RAT+E +
Sbjct 239 LCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHA 293
> sce:YIL042C PKP1; Mitochondrial protein kinase involved in negative
regulation of pyruvate dehydrogenase complex activity
by phosphorylating the ser-133 residue of the Pda1p subunit;
acts in concert with kinase Pkp2p and phosphatases Ptc5p
and Ptc6p (EC:2.7.11.2)
Length=394
Score = 45.4 bits (106), Expect = 6e-05, Method: Compositional matrix adjust.
Identities = 36/141 (25%), Positives = 65/141 (46%), Gaps = 11/141 (7%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRLSLDVSFDFFLERFFYFRIGRRVMVNNLLAMQ 60
F +LL+ H+ ++ +L + LQ+++ FL RI +++V + L++
Sbjct 141 FTELLDD----HEDAIVVLAKGLQEIQSCYPKFQISQFLNFHLKERITMKLLVTHYLSLM 196
Query 61 NPKPG-----WCGIVNPRCRPAEVIMARAQEVRDSC--KFSYGIAPLVVVAGNLETEFAT 113
G GI++ A++I + V D C KF+ P+++ + + F
Sbjct 197 AQNKGDTNKRMIGILHRDLPIAQLIKHVSDYVNDICFVKFNTQRTPVLIHPPSQDITFTC 256
Query 114 IPEHLALIITEVLKNALRATV 134
IP L I+TEV KNA A +
Sbjct 257 IPPILEYIMTEVFKNAFEAQI 277
> xla:379789 pdk4, 3j828, MGC132353, MGC52849; pyruvate dehydrogenase
kinase, isozyme 4 (EC:2.7.11.2); K00898 pyruvate dehydrogenase
kinase [EC:2.7.11.2]
Length=404
Score = 44.7 bits (104), Expect = 9e-05, Method: Compositional matrix adjust.
Identities = 37/151 (24%), Positives = 67/151 (44%), Gaps = 16/151 (10%)
Query 1 FVKLLEQERQQHDSSVDLLGRALQQLKRL-----SLDVSFDFFLERFFYFRIGRRVMVNN 55
F + L R +H+ V + + + + K + + +FL+RF+ RI R+++N
Sbjct 112 FTEALVTIRNRHNDVVPTMAQGVIEYKEAFGVDPVTNQNVQYFLDRFYMSRISIRMLINQ 171
Query 56 LLAM----QNP-KPGWCGIVNPRCRPAEVIMARAQEVRDSCKFSYGIAP-LVVVAGNLET 109
+ NP P G ++P EV+ + C+ Y +P L + N +
Sbjct 172 HTLLFDGGTNPAHPKHIGSIDPNSDVVEVVHDAFDTAKMLCEQYYLASPELRIKQANGKD 231
Query 110 -----EFATIPEHLALIITEVLKNALRATVE 135
+P HL ++ E+ KNA+RAT+E
Sbjct 232 PSQPIHIVYVPSHLYHMLFELFKNAMRATIE 262
> sce:YGL059W PKP2; Mitochondrial protein kinase that negatively
regulates activity of the pyruvate dehydrogenase complex
by phosphorylating the ser-133 residue of the Pda1p subunit;
acts in concert with kinase Pkp1p and phosphatases Ptc5p and
Ptc6p (EC:2.7.11.-)
Length=491
Score = 33.1 bits (74), Expect = 0.28, Method: Composition-based stats.
Identities = 12/39 (30%), Positives = 23/39 (58%), Gaps = 0/39 (0%)
Query 99 PLVVVAGNLETEFATIPEHLALIITEVLKNALRATVEFY 137
P ++ G+ + F +P HL ++ E+L+N AT++ Y
Sbjct 275 PEFIIEGDTQLSFYFLPTHLKYLLGEILRNTYEATMKHY 313
> hsa:100288524 forkhead box L1-like
Length=233
Score = 30.0 bits (66), Expect = 2.7, Method: Compositional matrix adjust.
Identities = 14/45 (31%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
Query 14 SSVDLLGRALQQ--LKRLSLDVSFDFFLERFFYFRIGRRVMVNNL 56
S + L+ A+QQ R++L +DF + +F Y+R +R N++
Sbjct 36 SYIALIAMAIQQSPAGRVTLSGIYDFIMRKFPYYRANQRAWQNSI 80
> mmu:384244 Gm5294, EG384244; predicted gene 5294
Length=216
Score = 29.6 bits (65), Expect = 3.3, Method: Compositional matrix adjust.
Identities = 14/45 (31%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
Query 14 SSVDLLGRALQQ--LKRLSLDVSFDFFLERFFYFRIGRRVMVNNL 56
S + L+ A+QQ R++L +DF + +F Y+R +R N++
Sbjct 36 SYIALIAMAIQQSPAGRVTLSGIYDFIMRKFPYYRANQRAWQNSI 80
> ath:AT4G23620 50S ribosomal protein-related
Length=264
Score = 29.3 bits (64), Expect = 4.4, Method: Compositional matrix adjust.
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 0/40 (0%)
Query 4 LLEQERQQHDSSVDLLGRALQQLKRLSLDVSFDFFLERFF 43
+ EQE QH + L+ Q+++L + + FFL R F
Sbjct 80 IFEQEDGQHGGNKRLISVQTNQIRKLVNHLGYSFFLSRLF 119
Lambda K H
0.327 0.141 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2618291680
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40