bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_4192_orf1
Length=267
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_019540 alanyl-tRNA synthetase, putative (EC:6.1.1.7... 194 3e-49
cpv:cgd6_560 alanyl-tRNA synthetase (with HxxxH domain) ; K018... 113 6e-25
tpv:TP01_1004 alanyl-tRNA synthetase; K01872 alanyl-tRNA synth... 106 8e-23
xla:100158432 aars; alanyl-tRNA synthetase (EC:6.1.1.7); K0187... 102 1e-21
mmu:234734 Aars, AI316495, C76919, MGC37368, sti; alanyl-tRNA ... 99.4 1e-20
hsa:16 AARS, CMT2N; alanyl-tRNA synthetase (EC:6.1.1.7); K0187... 96.7 8e-20
sce:YOR335C ALA1, CDC64; Ala1p (EC:6.1.1.7); K01872 alanyl-tRN... 90.9 5e-18
bbo:BBOV_III011650 17.m07993; alanyl-tRNA synthetase family pr... 89.7 1e-17
cel:F28H1.3 ars-2; Alanyl tRNA Synthetase family member (ars-2... 88.2 3e-17
ath:AT1G50200 ALATS; ALATS (ALANYL-TRNA SYNTHETASE); ATP bindi... 86.3 1e-16
pfa:PF13_0354 alanine--tRNA ligase, putative (EC:6.1.1.7); K01... 83.6 7e-16
eco:b2697 alaS, act, ECK2692, JW2667, lovB; alanyl-tRNA synthe... 70.5 6e-12
ath:AT5G22800 EMB1030 (EMBRYO DEFECTIVE 1030); ATP binding / a... 70.5 7e-12
dre:792787 im:7141191; si:dkey-240e12.1; K01872 alanyl-tRNA sy... 61.6 3e-09
mmu:224805 Aars2, Aarsl, AlaRS, Gm89, MGC69820; alanyl-tRNA sy... 55.5 2e-07
hsa:57505 AARS2, AARSL, KIAA1270, MT-ALARS, MTALARS, bA444E17.... 49.7 1e-05
dre:100331862 Alanyl tRNA Synthetase family member (ars-2)-like 45.4 2e-04
cel:W02B12.6 ars-1; Alanyl tRNA Synthetase family member (ars-... 42.7 0.001
hsa:80755 AARSD1, MGC2744; alanyl-tRNA synthetase domain conta... 34.7 0.34
mmu:69684 Aarsd1, 1110069E20Rik, 2310044P18Rik, AA589600, AlaX... 34.7 0.37
dre:445178 aarsd1, im:7152658, zgc:101066; alanyl-tRNA synthet... 32.7 1.2
mmu:68592 Syf2, 1110018L13Rik, Cbpin, D4Bwg1551e, Gcipip, mp29... 31.2 3.6
sce:YNL040W Putative protein of unknown function with strong s... 31.2 3.7
hsa:25949 SYF2, CBPIN, DKFZp564O2082, NTC31, P29, fSAP29; SYF2... 30.8 5.7
ath:AT5G24100 leucine-rich repeat transmembrane protein kinase... 30.4 6.3
ath:AT3G07690 NAD or NADH binding / binding / catalytic/ coenz... 30.4 6.4
mmu:101706 Numa1, 6720401E04Rik, AA764025, AL022610, AU014979;... 30.0 9.3
xla:379958 aarsd1, MGC2744, MGC52898, aarsd1-a, aarsd1-b; alan... 30.0 9.5
> tgo:TGME49_019540 alanyl-tRNA synthetase, putative (EC:6.1.1.7);
K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=1037
Score = 194 bits (493), Expect = 3e-49, Method: Compositional matrix adjust.
Identities = 116/244 (47%), Positives = 157/244 (64%), Gaps = 8/244 (3%)
Query 5 VVRPCVLILFQIASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQK 64
VV+ C IA + PV+ +PL AM++P L AVFGE YPDPVRVV++G D + L
Sbjct 728 VVKDC------IAQARPVHYKEVPLEQAMKIPGLRAVFGEVYPDPVRVVSVGEDIESLVA 781
Query 65 IQETQGADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSE 124
+ ++ + +S+ELCGGTHL NA+QL DF ++SEES+AKGVRR+VA AG+AA AR E
Sbjct 782 LADSSQPETPFASIELCGGTHLHNASQLSDFCILSEESVAKGVRRVVAAAGDAAFEARKE 841
Query 125 LRDVCLSLSNLESASPAFPFFEKEVQQAKAFVESNKMIPLLSRRRLLADLENKQKLGLEL 184
L ++ + +L SA EKEV +A+ ++ +PL+ +R L+ LE QK LEL
Sbjct 842 LEELEEAFRSLASAPRELAALEKEVAKARGLLDGAGPLPLVGKRSLVKQLEQAQKALLEL 901
Query 185 GKFRAKELLRVGKLSGQEAAAAAAAEGKKFIVLNLEQLQGDGKALDEAAKALQSAAPSLP 244
K R+KEL++ K G+E K F+VLNL QL GDGKALD A++ L AAP LP
Sbjct 902 QKARSKELVKEAKKLGEELGTEHKT--KPFLVLNLPQLLGDGKALDAASQTLHQAAPDLP 959
Query 245 FLLL 248
FLL+
Sbjct 960 FLLV 963
> cpv:cgd6_560 alanyl-tRNA synthetase (with HxxxH domain) ; K01872
alanyl-tRNA synthetase [EC:6.1.1.7]
Length=1003
Score = 113 bits (283), Expect = 6e-25, Method: Composition-based stats.
Identities = 83/245 (33%), Positives = 125/245 (51%), Gaps = 16/245 (6%)
Query 29 LRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVSSSVELCGGTHLSN 88
L A ++P + A+FGE YPDPVRV+++ L Q T D+ S+E CGGTH+ N
Sbjct 707 LSIAKQIPNIRAIFGETYPDPVRVLSVSKSVDSLINSQ-TDNNDQEKVSIEFCGGTHVEN 765
Query 89 AAQLEDFVVISEESIAKGVRRIVAVA---GEAAISARSELRDVCLSLSNLESASPAFPFF 145
+++ F +ISEE IAKG+RRIVAV E AI L+ L L
Sbjct 766 TSEIISFSIISEEGIAKGIRRIVAVTDNQSEIAIENAKRLKQEFKKLEELNGKE-----L 820
Query 146 EKEVQQAKAFVESNKMIPLLSRRRLLADLENKQKLGLELGKFRAKELLRVGK----LSGQ 201
E + K+ ++ K++PLL RR+L +E K + K + KE L + K GQ
Sbjct 821 ETLLNSFKSKLDETKLLPLLYRRQLSQIIEENYKRVIADAKKKQKERLNMAKELATKIGQ 880
Query 202 EAAAAAAAEGK-KFIVLNLEQLQGDGKALDEAAKALQSAAPSLPFLLLTXLE--SRGSSC 258
E + ++ + ++LN++Q+QGD K++D + + P P +L T E G S
Sbjct 881 EYSQQLSSNNEFPALILNIKQIQGDSKSMDVIGQDISKKYPEAPIMLFTSGEFNDNGVSV 940
Query 259 IXLSP 263
I + P
Sbjct 941 ISIMP 945
> tpv:TP01_1004 alanyl-tRNA synthetase; K01872 alanyl-tRNA synthetase
[EC:6.1.1.7]
Length=1011
Score = 106 bits (265), Expect = 8e-23, Method: Composition-based stats.
Identities = 76/277 (27%), Positives = 129/277 (46%), Gaps = 37/277 (13%)
Query 16 IASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVS 75
+ S P+ T + + A+E+P + A F E YP+ VRVV + +++
Sbjct 708 VRSGAPLRTTEIEYKKALEIPGIRANFDEKYPEVVRVVNVQMGSEDFSGEH--------- 758
Query 76 SSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCLSLSNL 135
+S E+CGGTHL+N ++D V+ EE I+KGVRR+ V + +R +L + L +L
Sbjct 759 TSTEVCGGTHLTNTKTIQDVAVVGEEGISKGVRRLTIVTNDQCRRSRIDLLNYYQRLGDL 818
Query 136 ESASPAFPFFEKEVQQA--------------KAFVESNKMIPLLSRRRLLADLENKQKLG 181
E + F + + ++A + VE +KM+PLLS+R L+ E L
Sbjct 819 EKSLKPINFTKDDTREASENASENMSKLTALRGEVEVHKMLPLLSKRNLMKKFEELIDLQ 878
Query 182 LELGKFRAKELLRVGKLSGQEAAAAAAAE---GKKFI--------VLN--LEQLQGDGKA 228
+ GK K L + K+ E ++ G +FI VL ++ L+GD K+
Sbjct 879 VSAGKAHQKRLANIAKIKAVEVVEKLQSQKYDGMEFIEKTSEHPAVLRAAVDDLEGDLKS 938
Query 229 LDEAAKALQSAAPSLPFLLLTXLESRGSSCIXLSPRA 265
L+ + + P L ++ + + + C SP A
Sbjct 939 LNVFVQTVSKTFPQLA-VVASSMGNNAVCCRVNSPNA 974
> xla:100158432 aars; alanyl-tRNA synthetase (EC:6.1.1.7); K01872
alanyl-tRNA synthetase [EC:6.1.1.7]
Length=968
Score = 102 bits (254), Expect = 1e-21, Method: Composition-based stats.
Identities = 77/251 (30%), Positives = 123/251 (49%), Gaps = 29/251 (11%)
Query 28 PLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVSSSVELCGGTHLS 87
PL TA + L AVF E YPDPVRVV+IG ++L + + +SVE CGGTHL
Sbjct 672 PLATAKAIQGLRAVFDETYPDPVRVVSIGIPVEDL--LADPSSPAGSVTSVEFCGGTHLK 729
Query 88 NAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCLSLSNLESASPAFPFFEK 147
N+ F++++EE+IAKG+RRIVAV G A A + +SLS+LE K
Sbjct 730 NSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDAIKVSLSDLEKKVKKQTDPNK 789
Query 148 EVQQAKAFVESNKMIPLLSR-------------RRLLADLENKQKLGLELGKFRAKELLR 194
+VQ+ A + N ++++ ++ + DL+ K ++ K +L
Sbjct 790 DVQKEIADLSENLATVVIAQWQKDVLREQLKTLKKTMDDLDRASKADVQ------KRVLE 843
Query 195 VGKLSGQEAAAAAAAEGKKFIVLNLEQLQGDGKALDEAAKALQSAAPSLPFLLLTXLESR 254
+ K +E + +VL +E KAL+E+ K L+S +P ++ +
Sbjct 844 ITKQLIEEHP------NQPLLVLEMEN-GASAKALNESLKLLKSQSPQTAAMIFSVDSDA 896
Query 255 GS-SCIXLSPR 264
G +C+ P+
Sbjct 897 GKITCLCQVPK 907
> mmu:234734 Aars, AI316495, C76919, MGC37368, sti; alanyl-tRNA
synthetase (EC:6.1.1.7); K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=968
Score = 99.4 bits (246), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 80/253 (31%), Positives = 129/253 (50%), Gaps = 9/253 (3%)
Query 16 IASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVS 75
I ++ PV T PL A + L AVF E YPDPVRVV+IG EL + + G
Sbjct 660 IEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSEL--LDDPCGPAGSL 717
Query 76 SSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCLSLSNL 135
+SVE CGGTHL N++ FV+++EE+IAKG+RRIVAV G A A + + SLS +
Sbjct 718 TSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAM 777
Query 136 ESASPAFPFFEKEVQQAKAFVE---SNKMIPLLSRRRLLADLENKQKLGLELGKFRAKEL 192
E+ A K+VQ+ A + + +IP + L++ +K+ +L + ++
Sbjct 778 EAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADV 837
Query 193 LRVGKLSGQEAAAAAAAEGKKFIVLNLEQLQGDGKALDEAAKALQSAAPSLPFLLLTXLE 252
+ ++ + + + ++L +E KAL+EA K ++ +P +L T
Sbjct 838 QK--RVLEKTKQLIDSNPNQPLVILEMES-GASAKALNEALKLFKTHSPQTSAMLFTVDN 894
Query 253 SRGS-SCIXLSPR 264
G +C+ P+
Sbjct 895 EAGKITCLCQVPQ 907
> hsa:16 AARS, CMT2N; alanyl-tRNA synthetase (EC:6.1.1.7); K01872
alanyl-tRNA synthetase [EC:6.1.1.7]
Length=968
Score = 96.7 bits (239), Expect = 8e-20, Method: Compositional matrix adjust.
Identities = 88/263 (33%), Positives = 127/263 (48%), Gaps = 29/263 (11%)
Query 16 IASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVS 75
I ++ V T PL A + L AVF E YPDPVRVV+IG EL + + G
Sbjct 660 IEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSEL--LDDPSGPAGSL 717
Query 76 SSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISA--RSELRDVCLSLS 133
+SVE CGGTHL N++ FV+++EE+IAKG+RRIVAV G A A ++E CLS+
Sbjct 718 TSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLKKCLSVM 777
Query 134 NLESASPAFPFFEKEVQQAKAFVE---SNKMIPLLSRRRLLADLENKQKLGLELGKFRAK 190
+ + P K+VQ+ A + + +IP + L L++ +K+
Sbjct 778 EAKVKAQTAP--NKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKV--------MD 827
Query 191 ELLRVGKLSGQEAAAAAAAEGKKFIVLN-------LEQLQG-DGKALDEAAKALQSAAPS 242
+L R K Q+ K+FI N LE G KAL+EA K + +P
Sbjct 828 DLDRASKADVQKRVLEKT---KQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQ 884
Query 243 LPFLLLTXLESRGS-SCIXLSPR 264
+L T G +C+ P+
Sbjct 885 TSAMLFTVDNEAGKITCLCQVPQ 907
> sce:YOR335C ALA1, CDC64; Ala1p (EC:6.1.1.7); K01872 alanyl-tRNA
synthetase [EC:6.1.1.7]
Length=958
Score = 90.9 bits (224), Expect = 5e-18, Method: Composition-based stats.
Identities = 51/137 (37%), Positives = 74/137 (54%), Gaps = 3/137 (2%)
Query 15 QIASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKV 74
QI +L V +PL A + + AVFGE YPDPVRVV++G +EL + +
Sbjct 655 QIKENLQVFYKEIPLDLAKSIDGVRAVFGETYPDPVRVVSVGKPIEEL--LANPANEEWT 712
Query 75 SSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCLSLSN 134
S+E CGGTH++ ++ FV++ E IAKG+RRIVAV G A A+ L
Sbjct 713 KYSIEFCGGTHVNKTGDIKYFVILEESGIAKGIRRIVAVTGTEAFEAQRLAEQFAADLDA 772
Query 135 LESASPAFPFFEKEVQQ 151
+ P P EK++++
Sbjct 773 ADKL-PFSPIKEKKLKE 788
> bbo:BBOV_III011650 17.m07993; alanyl-tRNA synthetase family
protein (EC:6.1.1.7); K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=978
Score = 89.7 bits (221), Expect = 1e-17, Method: Composition-based stats.
Identities = 72/256 (28%), Positives = 119/256 (46%), Gaps = 34/256 (13%)
Query 27 LPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVSSSVELCGGTHL 86
+ + A+E+P + A F + YP+ VRVV + TK+ Q I D + SVE+CGGTH+
Sbjct 688 IDFKDAVEIPGIRANFTDVYPEVVRVVAM---TKDGQPI------DGKAYSVEICGGTHV 738
Query 87 SNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCLSLSNLESASPAFP--- 143
L+ +++ EES++KGVRR+ E + +++ L L LES F
Sbjct 739 PFTGVLKSAIIVGEESVSKGVRRLTLATNEKSEDSKAILASYWQQLETLESELYKFSDGM 798
Query 144 FFEKEVQQA----------KAFVESNKMIPLLSRRRLLADLENKQKLGLELGKFRAKELL 193
QQA + ++S K++PLL RR L + +E GK K L
Sbjct 799 DTATMAQQANDNMKRLTALRFQMQSEKLLPLLGRRDLKNKFDTLINQQVEAGKVHQKRLG 858
Query 194 RVGK----------LSGQEAAAAAAAEGKKFIVLNLEQLQGDGKALDEAAKALQSAAPSL 243
+ K +G +A I ++ L+GD K+++ A+ L A P +
Sbjct 859 VIAKNMSTEYVNKFKAGDFGSAKVTLGTVDMIKMDANDLEGDVKSVNLFAQTLSKALPKM 918
Query 244 PFLLLTXLESRGSSCI 259
L+ + ++R S+C+
Sbjct 919 AILITS--KNRDSTCV 932
> cel:F28H1.3 ars-2; Alanyl tRNA Synthetase family member (ars-2);
K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=968
Score = 88.2 bits (217), Expect = 3e-17, Method: Composition-based stats.
Identities = 69/229 (30%), Positives = 114/229 (49%), Gaps = 18/229 (7%)
Query 28 PLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVSSSVELCGGTHLS 87
PL A ++ L A+F E YPDPVRVV +G ++L +Q + +++VE CGGTHL
Sbjct 673 PLGEAKKVKGLRAMFDETYPDPVRVVAVGTPVEQL--LQNPDAEEGQNTTVEFCGGTHLQ 730
Query 88 NAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCLSLS----------NLES 137
N + + V+ SEE+IAKG+RRIVA+ G A A + + L L +
Sbjct 731 NVSHIGRIVIASEEAIAKGIRRIVALTGPEAERAIARADRLTARLEEESKHADKKDELLA 790
Query 138 ASPAFPFFEKEVQQAKAFVESN-KMIPLLSRRRLLADLENKQKLGLELGKFRAKELLRVG 196
F +K++Q+ E+N +P + + + QK L+ G +A++
Sbjct 791 NKDKFKALQKKIQE--IVDEANGAQLPYWRKDSIREKAKAIQKT-LD-GYTKAQQAAVAE 846
Query 197 KLSGQEAAAAAAAEGKKFIVLNLEQLQGDGKALDEAAKALQSAAPSLPF 245
K+ G+ AA AE + +++++ + KA+D A K L+ + F
Sbjct 847 KVLGEAKELAAVAE-QPTVLVHVFAANANSKAIDNALKLLKDTKAVMAF 894
> ath:AT1G50200 ALATS; ALATS (ALANYL-TRNA SYNTHETASE); ATP binding
/ alanine-tRNA ligase/ ligase, forming aminoacyl-tRNA and
related compounds / nucleic acid binding / nucleotide binding
(EC:6.1.1.7); K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=1003
Score = 86.3 bits (212), Expect = 1e-16, Method: Compositional matrix adjust.
Identities = 48/107 (44%), Positives = 62/107 (57%), Gaps = 2/107 (1%)
Query 15 QIASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKV 74
QI L V + L A + L AVFGE YPDPVRVV+IG ++L E +
Sbjct 701 QIKDELDVFSKEAVLSEAKRIKGLRAVFGEVYPDPVRVVSIGRKVEDLLADPENNEWSLL 760
Query 75 SSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISA 121
SS E CGGTH++N + + F ++SEE IAKG+RR+ AV E A A
Sbjct 761 SS--EFCGGTHITNTREAKAFALLSEEGIAKGIRRVTAVTTECAFDA 805
> pfa:PF13_0354 alanine--tRNA ligase, putative (EC:6.1.1.7); K01872
alanyl-tRNA synthetase [EC:6.1.1.7]
Length=1408
Score = 83.6 bits (205), Expect = 7e-16, Method: Composition-based stats.
Identities = 68/255 (26%), Positives = 116/255 (45%), Gaps = 14/255 (5%)
Query 16 IASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVS 75
I L V + L + ++ + A+F E Y D V VV I K++ I D
Sbjct 1110 IKEELNVTVKTMDLDESKKIKGIRAIFEEDYADKVNVVFI---NKDVNNILNNMNIDYTY 1166
Query 76 -SSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCLSLSN 134
S+ELCGGTH+ N + F+V SEESI KG+ RI AV + A D+ +
Sbjct 1167 LHSIELCGGTHIGNTKLIRKFLVTSEESIGKGIYRITAVTNKKADEIEKTFNDLYDKYKH 1226
Query 135 LESASPAFPFFEKEVQQAKAFVESNKMIPLLSRRRLLADLENKQKLGLELGKFRAKELLR 194
+ +VQ K ++ NK +P + + +L +LE +K +E K KEL
Sbjct 1227 VLQEPNENKL--ADVQNYKRTLKENKFLPYIKKYHILQELEQIEKTIVEKTKNMQKELFN 1284
Query 195 VGKLSGQEAAAAAAAEGKKFIVLNL---EQLQGDGKALDEAAKALQSAAPSLPFLLLTXL 251
G++ A + K I+L++ ++++G+ K L++ ++ +L + +
Sbjct 1285 KATNIGKD----YAVQDKNNILLDIKFFDEIKGNQKVLEKIVQSYSKNNKNLSYFFIICD 1340
Query 252 ESRGSSCIXLSPRAL 266
E+ + C+ AL
Sbjct 1341 EN-NTYCVLEVKEAL 1354
> eco:b2697 alaS, act, ECK2692, JW2667, lovB; alanyl-tRNA synthetase
(EC:6.1.1.7); K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=876
Score = 70.5 bits (171), Expect = 6e-12, Method: Compositional matrix adjust.
Identities = 43/110 (39%), Positives = 57/110 (51%), Gaps = 19/110 (17%)
Query 11 LILFQIASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQG 70
L+ QI +LP+ T ++ L A A+ A+FGE Y + VRV+++G
Sbjct 615 LVNTQIRRNLPIETNIMDLEAAKAKGAM-ALFGEKYDERVRVLSMG-------------- 659
Query 71 ADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAIS 120
S ELCGGTH S + F +ISE A GVRRI AV GE AI+
Sbjct 660 ----DFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIA 705
> ath:AT5G22800 EMB1030 (EMBRYO DEFECTIVE 1030); ATP binding /
alanine-tRNA ligase/ ligase, forming aminoacyl-tRNA and related
compounds / nucleic acid binding / nucleotide binding (EC:6.1.1.7);
K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=978
Score = 70.5 bits (171), Expect = 7e-12, Method: Compositional matrix adjust.
Identities = 52/127 (40%), Positives = 68/127 (53%), Gaps = 24/127 (18%)
Query 11 LILFQIASSLPVNTAVLPLRTAMELPALCAVFGEAYP-DPVRVVTIGPDTKELQKIQETQ 69
LI I + + T VLPL A A+ A+FGE Y + VRVV E
Sbjct 706 LINRWIGDATRLETKVLPLADAKRAGAI-AMFGEKYDENEVRVV-------------EVP 751
Query 70 GADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAI----SARSEL 125
G S+ELCGGTH+ N A++ F +ISE+ IA G+RRI AVAGEA I S S++
Sbjct 752 GV-----SMELCGGTHVGNTAEIRAFKIISEQGIASGIRRIEAVAGEAFIEYINSRDSQM 806
Query 126 RDVCLSL 132
+C +L
Sbjct 807 TRLCSTL 813
> dre:792787 im:7141191; si:dkey-240e12.1; K01872 alanyl-tRNA
synthetase [EC:6.1.1.7]
Length=1004
Score = 61.6 bits (148), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 46/112 (41%), Positives = 63/112 (56%), Gaps = 6/112 (5%)
Query 11 LILFQIASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQG 70
L+L I + V+ +PL +A ++ L V E YPDPVRVV++ +L + +
Sbjct 693 LVLNIIRQNADVHVEEVPLSSAKQIAGLRTV-DELYPDPVRVVSVAVPVSDLLASERAE- 750
Query 71 ADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISAR 122
+SVELC GTHL + DFVV+SE + KGV RIVA G+ AI AR
Sbjct 751 ----QTSVELCCGTHLLRTGAIRDFVVVSERQMMKGVCRIVAFTGDDAIKAR 798
> mmu:224805 Aars2, Aarsl, AlaRS, Gm89, MGC69820; alanyl-tRNA
synthetase 2, mitochondrial (putative) (EC:6.1.1.7); K01872
alanyl-tRNA synthetase [EC:6.1.1.7]
Length=980
Score = 55.5 bits (132), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 54/182 (29%), Positives = 85/182 (46%), Gaps = 34/182 (18%)
Query 16 IASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVT--------IGPDTKELQKIQE 67
+ PV +PL +P L ++ E YPDPVRVV+ +GP ++
Sbjct 683 VGQDKPVFMEEVPLAHTARIPGLRSL-DEVYPDPVRVVSVGVPVAHALGPASQA------ 735
Query 68 TQGADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRD 127
+ +SVELC GTHL + + D V+I E + KG+ R++A+ GE A A R+
Sbjct 736 -----AMHTSVELCCGTHLLSTGAVGDLVIIGERQLVKGITRLLAITGEQAQQA----RE 786
Query 128 VCLSLSN-LESASPAFPFFEKEVQQAKAF---------VESNKMIPLLSRRRLLADLENK 177
V SLS +E+AS +++ +A V + +IP R+ L L+
Sbjct 787 VGQSLSQEVEAASERLSQGSRDLPEAHRLSKDIGRLTEVAESAVIPQWQRQELQTTLKML 846
Query 178 QK 179
Q+
Sbjct 847 QR 848
> hsa:57505 AARS2, AARSL, KIAA1270, MT-ALARS, MTALARS, bA444E17.1;
alanyl-tRNA synthetase 2, mitochondrial (putative) (EC:6.1.1.7);
K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=985
Score = 49.7 bits (117), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 35/96 (36%), Positives = 53/96 (55%), Gaps = 4/96 (4%)
Query 27 LPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQGADKVSSSVELCGGTHL 86
+PL ++P L ++ E YPDPVRVV++ + + + +SVELC GTHL
Sbjct 699 VPLALTAQVPGLRSL-DEVYPDPVRVVSV---GVPVAHALDPASQAALQTSVELCCGTHL 754
Query 87 SNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISAR 122
+ D V+I + ++KG R++AV GE A AR
Sbjct 755 LRTGAVGDLVIIGDRQLSKGTTRLLAVTGEQAQQAR 790
> dre:100331862 Alanyl tRNA Synthetase family member (ars-2)-like
Length=796
Score = 45.4 bits (106), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 35/112 (31%), Positives = 46/112 (41%), Gaps = 35/112 (31%)
Query 11 LILFQIASSLPVNTAVLPLRTAMELPALCAVFGEAYPDPVRVVTIGPDTKELQKIQETQG 70
L+L I + V+ +PL +A ++ L V E YPDPV
Sbjct 514 LVLNIIRQNADVHVEEVPLSSAKQIAGLRTV-DELYPDPV-------------------- 552
Query 71 ADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISAR 122
HL + DFVV+SE + KGV RIVA G+ AI AR
Sbjct 553 --------------HLLRTGAIRDFVVVSERQMMKGVCRIVAFTGDDAIKAR 590
> cel:W02B12.6 ars-1; Alanyl tRNA Synthetase family member (ars-1);
K01872 alanyl-tRNA synthetase [EC:6.1.1.7]
Length=793
Score = 42.7 bits (99), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 26/91 (28%), Positives = 46/91 (50%), Gaps = 18/91 (19%)
Query 50 VRVVTIGPDTKELQKIQETQGADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRR 109
VRVV +G GAD VE C GTH+ + ++D ++S++S+ + +RR
Sbjct 690 VRVVALGS------------GAD---VPVECCSGTHIHDVRVIDDVAIMSDKSMGQRLRR 734
Query 110 IVAVAGEAAISARSELRDVCLSLSNLESASP 140
I+ + G+ A + R+ + + +L S P
Sbjct 735 IIVLTGKEAAACRNYTKSI---YEDLRSTDP 762
> hsa:80755 AARSD1, MGC2744; alanyl-tRNA synthetase domain containing
1; K07050
Length=586
Score = 34.7 bits (78), Expect = 0.34, Method: Compositional matrix adjust.
Identities = 28/105 (26%), Positives = 45/105 (42%), Gaps = 26/105 (24%)
Query 15 QIASSLPVNTAVLPLRTAMELPALCAVFGEAYPD----PVRVVTIGPDTKELQKIQETQG 70
+I LPVN L +++ P + V G PD P+RVV I +G
Sbjct 335 KIRDRLPVNVREL----SLDDPEVEQVSGRGLPDDHAGPIRVVNI-------------EG 377
Query 71 ADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAG 115
D +C GTH+SN + L+ ++ E K ++ ++G
Sbjct 378 VDS-----NMCCGTHVSNLSDLQVIKILGTEKGKKNRTNLIFLSG 417
> mmu:69684 Aarsd1, 1110069E20Rik, 2310044P18Rik, AA589600, AlaX,
AlaXp-II; alanyl-tRNA synthetase domain containing 1; K07050
Length=412
Score = 34.7 bits (78), Expect = 0.37, Method: Compositional matrix adjust.
Identities = 30/105 (28%), Positives = 45/105 (42%), Gaps = 26/105 (24%)
Query 15 QIASSLPVNTAVLPLRTAMELPALCAVFGEAYPD----PVRVVTIGPDTKELQKIQETQG 70
+I LPV+ L L + P + V G PD P+RVVTI +G
Sbjct 161 KIRDRLPVSVRELSL----DDPEVEQVRGRGLPDDHAGPIRVVTI-------------EG 203
Query 71 ADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAG 115
D +C GTH+SN + L+ ++ E K ++ +AG
Sbjct 204 VDS-----NMCCGTHVSNLSDLQVIKILGTEKGKKNKSNLIFLAG 243
> dre:445178 aarsd1, im:7152658, zgc:101066; alanyl-tRNA synthetase
domain containing 1; K07050
Length=412
Score = 32.7 bits (73), Expect = 1.2, Method: Compositional matrix adjust.
Identities = 28/125 (22%), Positives = 54/125 (43%), Gaps = 26/125 (20%)
Query 15 QIASSLPVNTAVLPLRTAMELPALCAVFGEAYPD----PVRVVTIGPDTKELQKIQETQG 70
+I + +PV +L +++ PA+ V PD P+R++ I +G
Sbjct 160 KIRAHVPVTVNLL----SIDDPAVEKVRSRGLPDDHAGPIRIIDI-------------EG 202
Query 71 ADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCL 130
D +C GTH+SN + L+ ++ E K ++ +AG + + ++
Sbjct 203 VD-----ANMCCGTHVSNLSHLQVIKILGTEKGKKNKTNLIFIAGNRVLKYAEKSYNIEK 257
Query 131 SLSNL 135
SL+ L
Sbjct 258 SLTVL 262
> mmu:68592 Syf2, 1110018L13Rik, Cbpin, D4Bwg1551e, Gcipip, mp29,
p29; SYF2 homolog, RNA splicing factor (S. cerevisiae);
K12868 pre-mRNA-splicing factor SYF2
Length=242
Score = 31.2 bits (69), Expect = 3.6, Method: Compositional matrix adjust.
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 0/51 (0%)
Query 55 IGPDTKELQKIQETQGADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAK 105
I PD + ++ +E G D +S L GTH+ ++ +++ V+ E+ I K
Sbjct 141 IKPDMESYERQREKHGEDFFPTSNSLLHGTHVPSSEEIDRMVLDLEKQIEK 191
> sce:YNL040W Putative protein of unknown function with strong
similarity to alanyl-tRNA synthases from Eubacteria; green
fluorescent protein (GFP)-fusion protein localizes to the cytoplasm;
YNL040W is not an essential gene; K07050
Length=456
Score = 31.2 bits (69), Expect = 3.7, Method: Compositional matrix adjust.
Identities = 44/172 (25%), Positives = 74/172 (43%), Gaps = 23/172 (13%)
Query 42 FGEAYPDPVRVVTIGPDTKELQK-IQETQGADKVSSSVELCGGTHLSNAAQLEDFVVISE 100
GE D V I PD +L K + T + S+ C GTHL +Q+ +++S
Sbjct 203 IGEETVDEVSTSKI-PDDYDLSKGVLRTIHIGDIDSNP--CCGTHLKCTSQIGSILILSN 259
Query 101 ESIAKGVR-RIVAVAGE-AAISARSELRDVCLSLSNLESASPAFPFFEKEVQQAKAFVES 158
+S +G R+ + G+ ++ A+S + + L NL S S E Q ++
Sbjct 260 QSAVRGSNSRLYFMCGKRVSLYAKS-VNKILLDSKNLLSCS--------ETQISEKITRQ 310
Query 159 NKMIPLLSRRRLLADLENKQKLGLELGKFRAKELLRVGKLSGQEAAAAAAAE 210
K I L++R +Q L + ++EL+ K SG++ A E
Sbjct 311 TKQIQQLNKR--------EQYWIKRLARTASEELMNTLKASGKKRAYFMEEE 354
> hsa:25949 SYF2, CBPIN, DKFZp564O2082, NTC31, P29, fSAP29; SYF2
homolog, RNA splicing factor (S. cerevisiae); K12868 pre-mRNA-splicing
factor SYF2
Length=243
Score = 30.8 bits (68), Expect = 5.7, Method: Compositional matrix adjust.
Identities = 14/51 (27%), Positives = 28/51 (54%), Gaps = 0/51 (0%)
Query 55 IGPDTKELQKIQETQGADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAK 105
I PD + ++++E G + +S L GTH+ + +++ V+ E+ I K
Sbjct 142 IKPDMETYERLREKHGEEFFPTSNSLLHGTHVPSTEEIDRMVIDLEKQIEK 192
> ath:AT5G24100 leucine-rich repeat transmembrane protein kinase,
putative
Length=614
Score = 30.4 bits (67), Expect = 6.3, Method: Compositional matrix adjust.
Identities = 25/95 (26%), Positives = 47/95 (49%), Gaps = 9/95 (9%)
Query 44 EAYPDPVRVVTIGPDTKELQKIQETQGADKVSSSVELCG-----GTHLSNAAQLEDFVVI 98
E PD +++ P KE+ K+ + + + + E+ G++L A LED ++
Sbjct 285 EPKPDKLKLAKKMPSEKEVSKLGKEKNIEDMEDKSEINKVMFFEGSNL--AFNLEDLLIA 342
Query 99 SEESIAKGVRRIV--AVAGEAAISARSELRDVCLS 131
S E + KGV + AV ++ + A L+D+ +S
Sbjct 343 SAEFLGKGVFGMTYKAVLEDSKVIAVKRLKDIVVS 377
> ath:AT3G07690 NAD or NADH binding / binding / catalytic/ coenzyme
binding / glycerol-3-phosphate dehydrogenase (NAD+)/ oxidoreductase/
oxidoreductase, acting on CH-OH group of donors
/ oxidoreductase, acting on the CH-OH group of donors, NAD
or NADP as acceptor
Length=466
Score = 30.4 bits (67), Expect = 6.4, Method: Compositional matrix adjust.
Identities = 37/126 (29%), Positives = 57/126 (45%), Gaps = 15/126 (11%)
Query 22 VNTAVLPLRTAME-LPALCAVFGEAYPDPVRVVTIGPDTKE---LQKIQETQGA--DKVS 75
V T +P R E L + V G+A DP+R+V +G + +QE G KVS
Sbjct 6 VETNSVPSRLVEERLDEIRRVMGKADDDPLRIVGVGAGAWGSVFIAMLQENYGKFRGKVS 65
Query 76 SSVELCGGTHLSNAAQLEDFVVIS--EESIAKGVRR------IVAVAGEAAISARSELRD 127
+ GG + A F VI+ EE + + +RR + A G+ + A L+D
Sbjct 66 VRIWRRGGRAIDKATAEHLFEVINSREELLRRLIRRCAYLKYVEARLGDRVLYADEILKD 125
Query 128 -VCLSL 132
CL++
Sbjct 126 GFCLNM 131
> mmu:101706 Numa1, 6720401E04Rik, AA764025, AL022610, AU014979;
nuclear mitotic apparatus protein 1
Length=2094
Score = 30.0 bits (66), Expect = 9.3, Method: Compositional matrix adjust.
Identities = 25/95 (26%), Positives = 47/95 (49%), Gaps = 1/95 (1%)
Query 97 VISEESIAKGVRRIVAVAGEAAISARSELRDVCL-SLSNLESASPAFPFFEKEVQQAKAF 155
V+ +E +K ++R+V E + LR + + + SN A+ +EVQ +
Sbjct 1237 VLEKEGESKELKRLVVAESEKSQKLEERLRLLQVETASNSARAAERSSALREEVQSLREE 1296
Query 156 VESNKMIPLLSRRRLLADLENKQKLGLELGKFRAK 190
VE +++ SR+ L + E ++LG EL ++ K
Sbjct 1297 VEKQRVVSENSRQELASQAERAEELGQELKAWQEK 1331
> xla:379958 aarsd1, MGC2744, MGC52898, aarsd1-a, aarsd1-b; alanyl-tRNA
synthetase domain containing 1; K07050
Length=412
Score = 30.0 bits (66), Expect = 9.5, Method: Compositional matrix adjust.
Identities = 27/130 (20%), Positives = 52/130 (40%), Gaps = 26/130 (20%)
Query 15 QIASSLPVNTAVLPLRTAMELPALCAVFGEAYPD----PVRVVTIGPDTKELQKIQETQG 70
+I +PV+ ++ ++ P V PD PVR++ I +G
Sbjct 160 KIREHVPVHVRLI----TVDDPEFDMVRSRGLPDDHAGPVRIIDI-------------EG 202
Query 71 ADKVSSSVELCGGTHLSNAAQLEDFVVISEESIAKGVRRIVAVAGEAAISARSELRDVCL 130
D +C GTH+ N + L+ ++ E K ++ ++GE + S +
Sbjct 203 VD-----ANMCCGTHVRNLSDLQMIKILGTEKGKKNKTNLIFLSGERVLKYVSRSYNTEK 257
Query 131 SLSNLESASP 140
+L++L P
Sbjct 258 TLTSLLKNGP 267
Lambda K H
0.317 0.132 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 9954723792
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40