bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_5466_orf2
Length=126
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_026730 aconitate hydratase, putative (EC:4.2.1.3); ... 197 7e-51
tpv:TP01_1050 aconitate hydratase; K01681 aconitate hydratase ... 174 5e-44
xla:380269 aco1, MGC53330, ratireb; aconitase 1, soluble (EC:4... 168 4e-42
cel:ZK455.1 aco-1; ACOnitase family member (aco-1); K01681 aco... 160 1e-39
mmu:11428 Aco1, AI256519, Aco-1, Irebp, Irp1; aconitase 1 (EC:... 159 2e-39
ath:AT2G05710 aconitate hydratase, cytoplasmic, putative / cit... 159 2e-39
hsa:48 ACO1, ACONS, IREB1, IREBP, IREBP1, IRP1; aconitase 1, s... 157 1e-38
dre:568448 aco1, wu:fo75b03; aconitase 1, soluble (EC:4.2.1.3)... 157 1e-38
ath:AT4G35830 aconitate hydratase, cytoplasmic / citrate hydro... 155 3e-38
pfa:PF13_0229 aconitase (EC:4.2.1.3); K01681 aconitate hydrata... 152 2e-37
ath:AT4G26970 aconitate hydratase/ copper ion binding (EC:4.2.... 150 6e-37
bbo:BBOV_III011790 17.m08005; aconitate hydratase 1 family pro... 150 7e-37
dre:652982 ireb2, im:7153062; iron-responsive element binding ... 149 2e-36
eco:b1276 acnA, acn, ECK1271, JW1268; aconitate hydratase 1 (E... 139 1e-33
xla:414584 ireb2, MGC83131; iron-responsive element binding pr... 134 7e-32
mmu:64602 Ireb2, D9Ertd85e, DKFZp564D1164, Irp2; iron responsi... 132 2e-31
hsa:3658 IREB2, ACO3, FLJ23381, IRP2, IRP2AD; iron-responsive ... 131 4e-31
sce:YLR304C ACO1, GLU1; Aco1p (EC:4.2.1.3); K01681 aconitate h... 59.7 3e-09
xla:444692 aco2, MGC84375; aconitase 2, mitochondrial (EC:4.2.... 55.5 4e-08
sce:YJL200C ACO2; Aco2p (EC:4.2.1.3); K01681 aconitate hydrata... 55.1 6e-08
cel:F54H12.1 aco-2; ACOnitase family member (aco-2); K01681 ac... 54.7 7e-08
hsa:50 ACO2, ACONM, MGC20605, MGC33908; aconitase 2, mitochond... 54.3 1e-07
mmu:11429 Aco2, Aco-2, Aco3, D10Wsu183e; aconitase 2, mitochon... 53.1 2e-07
dre:322670 aco2, cb1017, wu:fa10e03, wu:fb69g04, wu:fc20c11; a... 52.4 3e-07
ath:AT5G54950 aconitate hydratase-related / citrate hydro-lyas... 47.0 1e-05
ath:AT2G43100 aconitase C-terminal domain-containing protein (... 36.2 0.027
xla:398139 aconitase; K01681 aconitate hydratase 1 [EC:4.2.1.3] 35.4 0.043
eco:b0071 leuD, ECK0073, JW0070; 3-isopropylmalate dehydratase... 34.7 0.079
cpv:cgd6_2300 Cut4/Apc1p/TSG24 family protein; meiotic check p... 33.5 0.16
sce:YGL009C LEU1; Isopropylmalate isomerase, catalyzes the sec... 32.3 0.34
eco:b0771 ybhJ, ECK0760, JW5103; predicted hydratase; K01681 a... 32.0 0.44
sce:YDR234W LYS4, LYS3; Homoaconitase, catalyzes the conversio... 31.2 0.76
dre:100003438 zgc:162322 30.8 1.2
tgo:TGME49_016710 hypothetical protein 29.3 2.9
xla:100337562 hspa5b, BiP/Grp78; heat shock 70 kDa protein 5b 28.9 4.0
dre:100332566 protocadherin gamma cluster 1 member-like 28.9 4.5
dre:449546 ric8a, zgc:92294; resistance to inhibitors of choli... 28.5 4.8
xla:397850 hspa5, hspa5a; heat shock 70 kDa protein 5a; K09490... 28.5 4.9
xla:379756 hspa5, BiP, MGC52648, grp78; heat shock 70kDa prote... 28.5 5.0
dre:30122 wnt8a, wnt-8, wnt8, wu:fa20e02, wu:fe05d07; wingless... 28.5 5.7
ath:AT5G57160 ATLIG4; ATLIG4; DNA ligase (ATP)/ protein bindin... 28.5 6.2
> tgo:TGME49_026730 aconitate hydratase, putative (EC:4.2.1.3);
K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=1055
Score = 197 bits (501), Expect = 7e-51, Method: Compositional matrix adjust.
Identities = 88/125 (70%), Positives = 112/125 (89%), Gaps = 0/125 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGKEYGSGSSRDWAAKGPYL GVKA+IA+SFERIHR+NLVGMGILPLQF +G++AE+
Sbjct 928 LAGKEYGSGSSRDWAAKGPYLMGVKAIIAESFERIHRTNLVGMGILPLQFQEGQNAESLG 987
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
LTGKE+F I+LN G+++PGS++ VKT GK+F+ +CRIDT++EV+YF+NGG LHYVLRN+
Sbjct 988 LTGKEQFNISLNKGEIIPGSLMTVKTSDGKAFDVRCRIDTELEVKYFQNGGILHYVLRNL 1047
Query 121 LNRNS 125
++S
Sbjct 1048 TKQHS 1052
> tpv:TP01_1050 aconitate hydratase; K01681 aconitate hydratase
1 [EC:4.2.1.3]
Length=912
Score = 174 bits (441), Expect = 5e-44, Method: Compositional matrix adjust.
Identities = 80/120 (66%), Positives = 102/120 (85%), Gaps = 1/120 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+AGKEYG+GSSRDWAAKGP L GVKA++A+SFERIHR+NLVG GILPLQFL G++A T +
Sbjct 790 VAGKEYGTGSSRDWAAKGPLLLGVKAILAESFERIHRTNLVGCGILPLQFLDGQNATTLN 849
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
LTG E+FT+ L + +VPGS++RV TD G SF+TKCR+DT +E EY+++GG L YVLR+I
Sbjct 850 LTGTEKFTVHLGS-DVVPGSLVRVTTDTGLSFDTKCRVDTQIESEYYKHGGILQYVLRSI 908
> xla:380269 aco1, MGC53330, ratireb; aconitase 1, soluble (EC:4.2.1.3);
K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=891
Score = 168 bits (426), Expect = 4e-42, Method: Compositional matrix adjust.
Identities = 78/122 (63%), Positives = 98/122 (80%), Gaps = 1/122 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
L GKEYGSGSSRDWAAKGP+LQG+KAV+A+S+ERIHRSNLVGMGI+PLQ+L GESAE
Sbjct 769 LTGKEYGSGSSRDWAAKGPFLQGIKAVLAESYERIHRSNLVGMGIIPLQYLPGESAEALG 828
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
L+G+ER+TI + L PG + +K D GKSF+ R DTDVE+ Y+RNGG L+Y++R +
Sbjct 829 LSGRERYTIIIPE-DLRPGMNVEIKLDTGKSFDAIMRFDTDVELTYYRNGGILNYMIRKM 887
Query 121 LN 122
N
Sbjct 888 AN 889
> cel:ZK455.1 aco-1; ACOnitase family member (aco-1); K01681 aconitate
hydratase 1 [EC:4.2.1.3]
Length=887
Score = 160 bits (404), Expect = 1e-39, Method: Compositional matrix adjust.
Identities = 73/121 (60%), Positives = 95/121 (78%), Gaps = 1/121 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGKEYG GSSRDWAAKGP+LQGVKAVIA+SFERIHRSNL+GMGI+P Q+ G++A++
Sbjct 767 LAGKEYGCGSSRDWAAKGPFLQGVKAVIAESFERIHRSNLIGMGIIPFQYQAGQNADSLG 826
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
LTGKE+F+I + + L PG +I V G F+ CR DT+VE+ Y+RNGG L Y++R +
Sbjct 827 LTGKEQFSIGVPD-DLKPGQLIDVNVSNGSVFQVICRFDTEVELTYYRNGGILQYMIRKL 885
Query 121 L 121
+
Sbjct 886 I 886
> mmu:11428 Aco1, AI256519, Aco-1, Irebp, Irp1; aconitase 1 (EC:4.2.1.3);
K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=889
Score = 159 bits (403), Expect = 2e-39, Method: Compositional matrix adjust.
Identities = 72/120 (60%), Positives = 97/120 (80%), Gaps = 1/120 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGKEYGSGSSRDWAAKGP+L G+KAV+A+S+ERIHRSNLVGMG++PL++L GE+A++
Sbjct 769 LAGKEYGSGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGETADSLG 828
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
LTG+ER+TI + L P +++K D GK+F+ R DTDVE+ YF NGG L+Y++R +
Sbjct 829 LTGRERYTINIPE-DLKPRMTVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKM 887
> ath:AT2G05710 aconitate hydratase, cytoplasmic, putative / citrate
hydro-lyase/aconitase, putative (EC:4.2.1.3); K01681
aconitate hydratase 1 [EC:4.2.1.3]
Length=990
Score = 159 bits (402), Expect = 2e-39, Method: Compositional matrix adjust.
Identities = 80/122 (65%), Positives = 92/122 (75%), Gaps = 2/122 (1%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAG EYGSGSSRDWAAKGP LQGVKAVIA+SFERIHRSNLVGMGI+PL F GE A+T
Sbjct 866 LAGAEYGSGSSRDWAAKGPMLQGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADTLG 925
Query 61 LTGKERFTIALNN--GKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLR 118
LTG ER+TI L ++ PG + V TD GKSF R DT+VE+ YF +GG L YV+R
Sbjct 926 LTGHERYTIHLPTDISEIRPGQDVTVTTDNGKSFTCTVRFDTEVELAYFNHGGILPYVIR 985
Query 119 NI 120
N+
Sbjct 986 NL 987
> hsa:48 ACO1, ACONS, IREB1, IREBP, IREBP1, IRP1; aconitase 1,
soluble (EC:4.2.1.3); K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=889
Score = 157 bits (396), Expect = 1e-38, Method: Compositional matrix adjust.
Identities = 72/120 (60%), Positives = 96/120 (80%), Gaps = 1/120 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGKEYG+GSSRDWAAKGP+L G+KAV+A+S+ERIHRSNLVGMG++PL++L GE+A+
Sbjct 769 LAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALG 828
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
LTG+ER+TI + L P ++VK D GK+F+ R DTDVE+ YF NGG L+Y++R +
Sbjct 829 LTGQERYTIIIPE-NLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKM 887
> dre:568448 aco1, wu:fo75b03; aconitase 1, soluble (EC:4.2.1.3);
K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=890
Score = 157 bits (396), Expect = 1e-38, Method: Compositional matrix adjust.
Identities = 70/120 (58%), Positives = 97/120 (80%), Gaps = 1/120 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGKEYGSGSSRDWAAKGP+L G+KAV+A+S+ERIHRSNLVGMG++PL++L G+SAE+
Sbjct 769 LAGKEYGSGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGDSAESLG 828
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
L+G+ER+T+ + L P + +K D GK+F+ + R DTDVE+ YF +GG L+Y++R +
Sbjct 829 LSGRERYTVMI-PPLLKPRMTVDIKLDTGKTFQARMRFDTDVELTYFHHGGILNYMIRKM 887
> ath:AT4G35830 aconitate hydratase, cytoplasmic / citrate hydro-lyase
/ aconitase (ACO); K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=795
Score = 155 bits (392), Expect = 3e-38, Method: Compositional matrix adjust.
Identities = 78/125 (62%), Positives = 94/125 (75%), Gaps = 2/125 (1%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAG EYGSGSSRDWAAKGP L GVKAVI++SFERIHRSNLVGMGI+PL F GE AET
Sbjct 671 LAGAEYGSGSSRDWAAKGPMLLGVKAVISKSFERIHRSNLVGMGIIPLCFKAGEDAETLG 730
Query 61 LTGKERFTIALNN--GKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLR 118
LTG+E +TI L N ++ PG + V T+ GKSF R DT+VE+ YF +GG L YV+R
Sbjct 731 LTGQELYTIELPNNVSEIKPGQDVTVVTNNGKSFTCTLRFDTEVELAYFDHGGILQYVIR 790
Query 119 NILNR 123
N++ +
Sbjct 791 NLIKQ 795
> pfa:PF13_0229 aconitase (EC:4.2.1.3); K01681 aconitate hydratase
1 [EC:4.2.1.3]
Length=909
Score = 152 bits (385), Expect = 2e-37, Method: Compositional matrix adjust.
Identities = 73/126 (57%), Positives = 96/126 (76%), Gaps = 3/126 (2%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+AGKEYG GSSRDWAAKGP L GVKAVIA+S+ERIHRSNL+GM +LPLQF+ +S + ++
Sbjct 781 IAGKEYGCGSSRDWAAKGPNLLGVKAVIAESYERIHRSNLIGMSVLPLQFINNQSPQYYN 840
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDC-GK--SFETKCRIDTDVEVEYFRNGGALHYVL 117
+ G E+FTI LN+G + I+V+ + GK F+ CRIDT++E YFRNGG L YVL
Sbjct 841 MDGTEKFTILLNDGNIKAQQTIKVQMNQKGKIIIFDVLCRIDTEIEERYFRNGGILKYVL 900
Query 118 RNILNR 123
R+++N
Sbjct 901 RSLVNE 906
> ath:AT4G26970 aconitate hydratase/ copper ion binding (EC:4.2.1.3);
K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=995
Score = 150 bits (380), Expect = 6e-37, Method: Compositional matrix adjust.
Identities = 76/122 (62%), Positives = 89/122 (72%), Gaps = 2/122 (1%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAG EYGSGSSRDWAAKGP L GVKAVIA+SFERIHRSNL GMGI+PL F GE AET
Sbjct 871 LAGAEYGSGSSRDWAAKGPLLLGVKAVIAKSFERIHRSNLAGMGIIPLCFKAGEDAETLG 930
Query 61 LTGKERFTIALNN--GKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLR 118
LTG ER+T+ L + PG + V TD GKSF R DT+VE+ Y+ +GG L YV+R
Sbjct 931 LTGHERYTVHLPTKVSDIRPGQDVTVTTDSGKSFVCTLRFDTEVELAYYDHGGILPYVIR 990
Query 119 NI 120
++
Sbjct 991 SL 992
> bbo:BBOV_III011790 17.m08005; aconitate hydratase 1 family protein
(EC:4.2.1.3); K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=908
Score = 150 bits (380), Expect = 7e-37, Method: Compositional matrix adjust.
Identities = 71/120 (59%), Positives = 91/120 (75%), Gaps = 1/120 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+AGKEYGSGSSRDWAAKGP L G++A+ A+SFERIHR+NLVG GILPLQF+ GE+A +
Sbjct 788 VAGKEYGSGSSRDWAAKGPALLGIRAIFAESFERIHRTNLVGFGILPLQFMPGENAASVG 847
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
+TG+E+FTI KL PG + V D G F +CRIDT +E++Y+++GG L YVL I
Sbjct 848 ITGREKFTID-GLDKLSPGCQVEVVADTGIKFNMRCRIDTALELQYYQHGGILQYVLARI 906
> dre:652982 ireb2, im:7153062; iron-responsive element binding
protein 2
Length=896
Score = 149 bits (377), Expect = 2e-36, Method: Compositional matrix adjust.
Identities = 74/121 (61%), Positives = 93/121 (76%), Gaps = 1/121 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGKEYGSGSSRDWAAKGPYL GV+AVIA+SFE+IHR++LVGMGI PLQFL G++A++
Sbjct 776 LAGKEYGSGSSRDWAAKGPYLLGVRAVIAESFEKIHRNHLVGMGIAPLQFLPGQNADSLE 835
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
L GKERFTI + +L I V+T GKSF + D++VE+FR+GG L YV R++
Sbjct 836 LCGKERFTIDIPE-ELTARQQITVQTSTGKSFMVTALFENDMDVEFFRHGGILKYVARSL 894
Query 121 L 121
L
Sbjct 895 L 895
> eco:b1276 acnA, acn, ECK1271, JW1268; aconitate hydratase 1
(EC:4.2.1.3); K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=891
Score = 139 bits (351), Expect = 1e-33, Method: Compositional matrix adjust.
Identities = 69/125 (55%), Positives = 89/125 (71%), Gaps = 5/125 (4%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+AGKEYGSGSSRDWAAKGP L G++ VIA+SFERIHRSNL+GMGILPL+F +G + +T
Sbjct 767 IAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLG 826
Query 61 LTGKERFTIA----LNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYV 116
LTG+E+ I L G VP ++ R + +CRIDT E+ Y++N G LHYV
Sbjct 827 LTGEEKIDIGDLQNLQPGATVPVTLTRAD-GSQEVVPCRCRIDTATELTYYQNDGILHYV 885
Query 117 LRNIL 121
+RN+L
Sbjct 886 IRNML 890
> xla:414584 ireb2, MGC83131; iron-responsive element binding
protein 2
Length=955
Score = 134 bits (337), Expect = 7e-32, Method: Compositional matrix adjust.
Identities = 64/121 (52%), Positives = 88/121 (72%), Gaps = 1/121 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+AGK+YG G+SRDWAAKGP+L GV+ VIA+S+E+IH+ +LVGMGI PLQFL GE+AET
Sbjct 836 IAGKKYGLGNSRDWAAKGPFLLGVRVVIAESYEKIHKDHLVGMGIAPLQFLSGENAETLG 895
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
L+GKE+++++L L PG + +KT+ GK F D + EV +++GG L YV R
Sbjct 896 LSGKEQYSLSL-PVDLTPGHKVEIKTNTGKIFHVIAAFDNEAEVTLYKHGGILSYVARKY 954
Query 121 L 121
L
Sbjct 955 L 955
> mmu:64602 Ireb2, D9Ertd85e, DKFZp564D1164, Irp2; iron responsive
element binding protein 2
Length=963
Score = 132 bits (333), Expect = 2e-31, Method: Compositional matrix adjust.
Identities = 61/121 (50%), Positives = 89/121 (73%), Gaps = 1/121 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGK+YGSG+SRDWAAKGPYL GVKAV+A+S+E+IH+ +L+G+GI PL+FL GE+A++
Sbjct 844 LAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLEFLPGENADSLG 903
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLRNI 120
L+G+E F+++ +L PG + +KT GK F DVE+ +++GG L++V R
Sbjct 904 LSGREVFSLSFPE-ELFPGITLNIKTSTGKEFSVIASFANDVEITLYKHGGLLNFVARKF 962
Query 121 L 121
L
Sbjct 963 L 963
> hsa:3658 IREB2, ACO3, FLJ23381, IRP2, IRP2AD; iron-responsive
element binding protein 2
Length=963
Score = 131 bits (330), Expect = 4e-31, Method: Compositional matrix adjust.
Identities = 60/118 (50%), Positives = 88/118 (74%), Gaps = 1/118 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LAGK+YGSG+SRDWAAKGPYL GVKAV+A+S+E+IH+ +L+G+GI PLQFL GE+A++
Sbjct 844 LAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLQFLPGENADSLG 903
Query 61 LTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVLR 118
L+G+E F++ +L PG + ++T GK F + DVE+ +++GG L++V R
Sbjct 904 LSGRETFSLTFPE-ELSPGITLNIQTSTGKVFSVIASFEDDVEITLYKHGGLLNFVAR 960
> sce:YLR304C ACO1, GLU1; Aco1p (EC:4.2.1.3); K01681 aconitate
hydratase 1 [EC:4.2.1.3]
Length=778
Score = 59.7 bits (143), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 40/119 (33%), Positives = 61/119 (51%), Gaps = 6/119 (5%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+ + +G GSSR+ AA P G A+I +SF RIH +NL G+LPL F A+
Sbjct 657 IGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNF--KNPADYDK 714
Query 61 LTGKERFTIALNNGKLVPGSII--RVKTDCGKSFETK-CRIDTDVEVEYFRNGGALHYV 116
+ +R I L +L PG + RV GK ++ D ++E+F+ G AL+ +
Sbjct 715 INPDDRIDI-LGLAELAPGKPVTMRVHPKNGKPWDAVLTHTFNDEQIEWFKYGSALNKI 772
> xla:444692 aco2, MGC84375; aconitase 2, mitochondrial (EC:4.2.1.3);
K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=782
Score = 55.5 bits (132), Expect = 4e-08, Method: Compositional matrix adjust.
Identities = 39/117 (33%), Positives = 64/117 (54%), Gaps = 6/117 (5%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+ + YG GSSR+ AA P G +A+I +SF RIH +NL G+LPL F + A+
Sbjct 662 IGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTF--SDPADYDK 719
Query 61 LTGKERFTIALNNGKLVPGSIIR-VKTDCGKSFETKCRIDT--DVEVEYFRNGGALH 114
+ +++ T+A L PG ++ + T S ET T + ++++F+ G AL+
Sbjct 720 IHPEDKITLA-GLKDLAPGKPVKCIITHQNGSQETIILNHTFNETQLQWFQAGSALN 775
> sce:YJL200C ACO2; Aco2p (EC:4.2.1.3); K01681 aconitate hydratase
1 [EC:4.2.1.3]
Length=789
Score = 55.1 bits (131), Expect = 6e-08, Method: Compositional matrix adjust.
Identities = 40/126 (31%), Positives = 65/126 (51%), Gaps = 15/126 (11%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+A YG GS+R+ AA P G + ++ +SF RIH +NL G+LPL F ES
Sbjct 662 IAEHNYGEGSAREHAALSPRFLGGEILLVKSFARIHETNLKKQGVLPLTF-ANESDYDKI 720
Query 61 LTGKERFTIAL--------NNGKLVPGSI-IRVKTDCGKSFETKCR-IDTDVEVEYFRNG 110
+G T+ L NNG G I +++ G+SF K + + ++++F+ G
Sbjct 721 SSGDVLETLNLVDMIAKDGNNG----GEIDVKITKPNGESFTIKAKHTMSKDQIDFFKAG 776
Query 111 GALHYV 116
A++Y+
Sbjct 777 SAINYI 782
> cel:F54H12.1 aco-2; ACOnitase family member (aco-2); K01681
aconitate hydratase 1 [EC:4.2.1.3]
Length=777
Score = 54.7 bits (130), Expect = 7e-08, Method: Compositional matrix adjust.
Identities = 41/120 (34%), Positives = 61/120 (50%), Gaps = 12/120 (10%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFL------KGE 54
+ + YG GSSR+ AA P G +A+I +SF RIH +NL G+LPL F K +
Sbjct 654 IGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGMLPLTFANPADYDKID 713
Query 55 SAETHSLTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALH 114
++ S+ G F GK P + I KT+ K T + ++E+F+ G AL+
Sbjct 714 PSDNVSIVGLSSFA----PGK--PLTAIFKKTNGSKVEVTLNHTFNEQQIEWFKAGSALN 767
> hsa:50 ACO2, ACONM, MGC20605, MGC33908; aconitase 2, mitochondrial
(EC:4.2.1.3); K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=780
Score = 54.3 bits (129), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 36/118 (30%), Positives = 61/118 (51%), Gaps = 8/118 (6%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+ + YG GSSR+ AA P G +A+I +SF RIH +NL G+LPL F + A+ +
Sbjct 660 IGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTF--ADPADYNK 717
Query 61 LTGKERFTI-ALNNGKLVPGSIIRVKTDCGKSFETKCRID---TDVEVEYFRNGGALH 114
+ ++ TI L + PG ++ + ++ + ++E+FR G AL+
Sbjct 718 IHPVDKLTIQGLKD--FTPGKPLKCIIKHPNGTQETILLNHTFNETQIEWFRAGSALN 773
> mmu:11429 Aco2, Aco-2, Aco3, D10Wsu183e; aconitase 2, mitochondrial
(EC:4.2.1.3); K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=780
Score = 53.1 bits (126), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 35/118 (29%), Positives = 61/118 (51%), Gaps = 8/118 (6%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
+ + YG GSSR+ AA P G +A+I +SF RIH +NL G+LPL F + ++ +
Sbjct 660 IGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTF--ADPSDYNK 717
Query 61 LTGKERFTI-ALNNGKLVPGSIIRVKTDCGKSFETKCRID---TDVEVEYFRNGGALH 114
+ ++ TI L + PG ++ + ++ + ++E+FR G AL+
Sbjct 718 IHPVDKLTIQGLKD--FAPGKPLKCVIKHPNGTQETILLNHTFNETQIEWFRAGSALN 773
> dre:322670 aco2, cb1017, wu:fa10e03, wu:fb69g04, wu:fc20c11;
aconitase 2, mitochondrial (EC:4.2.1.3); K01681 aconitate hydratase
1 [EC:4.2.1.3]
Length=782
Score = 52.4 bits (124), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 35/115 (30%), Positives = 60/115 (52%), Gaps = 8/115 (6%)
Query 4 KEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHSLTG 63
+ YG GSSR+ AA P G +A+I +SF RIH +NL G+LPL F A+ +
Sbjct 665 ENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGLLPLTF--SNPADYDKIRP 722
Query 64 KERFTIALNNGKLVPG----SIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALH 114
++ +I + PG ++I+ ++ E + + ++E+F+ G AL+
Sbjct 723 DDKISI-IGLKSFAPGKPLKAVIKHTDGSSETIELNHTFN-ETQIEWFQAGSALN 775
> ath:AT5G54950 aconitate hydratase-related / citrate hydro-lyase-related
/ aconitase-related
Length=74
Score = 47.0 bits (110), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 28/62 (45%), Positives = 35/62 (56%), Gaps = 2/62 (3%)
Query 48 LQFLKGESAETHSLTGKERFTIAL--NNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVE 105
+ F GE AET LTG E +TI L N ++ PG I V TD KSF R+DT++ V
Sbjct 1 MAFKSGEDAETLGLTGHELYTIHLPSNINEIKPGQDITVTTDTAKSFVCTLRLDTEIWVL 60
Query 106 YF 107
F
Sbjct 61 CF 62
> ath:AT2G43100 aconitase C-terminal domain-containing protein
(EC:4.2.1.33); K01704 3-isopropylmalate/(R)-2-methylmalate
dehydratase small subunit [EC:4.2.1.33 4.2.1.35]
Length=256
Score = 36.2 bits (82), Expect = 0.027, Method: Compositional matrix adjust.
Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMG-ILPLQ 49
+ G+ +G GSSR+ A G KA++A+S+ RI N V G + PL+
Sbjct 141 IGGENFGCGSSREHAPVCLGAAGAKAIVAESYARIFFRNSVATGEVFPLE 190
> xla:398139 aconitase; K01681 aconitate hydratase 1 [EC:4.2.1.3]
Length=856
Score = 35.4 bits (80), Expect = 0.043, Method: Compositional matrix adjust.
Identities = 18/46 (39%), Positives = 27/46 (58%), Gaps = 0/46 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGIL 46
+ + YG GSSR+ AA P G +A+I +SF RIH + + + L
Sbjct 760 IGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHPEDKITLAGL 805
Score = 32.0 bits (71), Expect = 0.52, Method: Compositional matrix adjust.
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 0/35 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERI 35
+ + YG GSSR+ AA P G +A+I +SF RI
Sbjct 663 IGDENYGEGSSREHAALEPRHLGGRAIITKSFARI 697
> eco:b0071 leuD, ECK0073, JW0070; 3-isopropylmalate dehydratase
small subunit (EC:4.2.1.33); K01704 3-isopropylmalate/(R)-2-methylmalate
dehydratase small subunit [EC:4.2.1.33 4.2.1.35]
Length=201
Score = 34.7 bits (78), Expect = 0.079, Method: Compositional matrix adjust.
Identities = 22/62 (35%), Positives = 29/62 (46%), Gaps = 0/62 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHS 60
LA + +G GSSR+ A G K VIA SF I N +LP++ E E +
Sbjct 75 LARENFGCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLLPVKLSDAEVDELFA 134
Query 61 LT 62
L
Sbjct 135 LV 136
> cpv:cgd6_2300 Cut4/Apc1p/TSG24 family protein; meiotic check
point regulator and 26S proteasome regulatory complex; PC-rep
repeats
Length=2006
Score = 33.5 bits (75), Expect = 0.16, Method: Composition-based stats.
Identities = 19/47 (40%), Positives = 28/47 (59%), Gaps = 6/47 (12%)
Query 11 SRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAE 57
S+ WA+ YL+ K F+RI SNL+ GI+P +FL+G+ E
Sbjct 539 SKFWAS---YLEKNKK---SPFDRIEFSNLISKGIIPHEFLEGKKYE 579
> sce:YGL009C LEU1; Isopropylmalate isomerase, catalyzes the second
step in the leucine biosynthesis pathway (EC:4.2.1.33);
K01702 3-isopropylmalate dehydratase [EC:4.2.1.33]
Length=779
Score = 32.3 bits (72), Expect = 0.34, Method: Compositional matrix adjust.
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 0/50 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQF 50
+ G +G GSSR+ A G+K++IA S+ I +N G+LP++
Sbjct 620 VTGDNFGCGSSREHAPWALKDFGIKSIIAPSYGDIFYNNSFKNGLLPIRL 669
> eco:b0771 ybhJ, ECK0760, JW5103; predicted hydratase; K01681
aconitate hydratase 1 [EC:4.2.1.3]
Length=753
Score = 32.0 bits (71), Expect = 0.44, Method: Compositional matrix adjust.
Identities = 24/78 (30%), Positives = 39/78 (50%), Gaps = 5/78 (6%)
Query 7 GSGSSRDWAAKGPYLQGVKAVIAQSFE-RIHRSNLVGMGILPLQFLKGESAETHSLTGKE 65
G GS+R+ AA + G A IA+ + + +RSN++ G+LPLQ + + E
Sbjct 638 GDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIP 697
Query 66 RFTIALNNGKLVPGSIIR 83
AL+N PG+ +
Sbjct 698 GIKAALDN----PGTTFK 711
> sce:YDR234W LYS4, LYS3; Homoaconitase, catalyzes the conversion
of homocitrate to homoisocitrate, which is a step in the
lysine biosynthesis pathway (EC:4.2.1.36); K01705 homoaconitate
hydratase [EC:4.2.1.36]
Length=693
Score = 31.2 bits (69), Expect = 0.76, Method: Compositional matrix adjust.
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 0/50 (0%)
Query 1 LAGKEYGSGSSRDWAAKGPYLQGVKAVIAQSFERIHRSNLVGMGILPLQF 50
++G +G+GSSR+ AA +G+ V++ SF I N + +L L+
Sbjct 568 VSGFNFGTGSSREQAATALLAKGINLVVSGSFGNIFSRNSINNALLTLEI 617
> dre:100003438 zgc:162322
Length=970
Score = 30.8 bits (68), Expect = 1.2, Method: Compositional matrix adjust.
Identities = 17/46 (36%), Positives = 28/46 (60%), Gaps = 2/46 (4%)
Query 60 SLTGKERFTIALNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVE 105
S +GK+ F++ L NG+L+ II +T CG+S +C + V +E
Sbjct 68 SESGKQYFSLNLRNGELLVNEIIDRETLCGQS--ARCVLPLQVIIE 111
> tgo:TGME49_016710 hypothetical protein
Length=593
Score = 29.3 bits (64), Expect = 2.9, Method: Composition-based stats.
Identities = 14/47 (29%), Positives = 22/47 (46%), Gaps = 0/47 (0%)
Query 71 LNNGKLVPGSIIRVKTDCGKSFETKCRIDTDVEVEYFRNGGALHYVL 117
L+ L PG + VK G + + + YF +GGA+H+ L
Sbjct 165 LSGANLFPGHVATVKAVLGAALSCAFVVPIAIRSVYFHSGGAVHHRL 211
> xla:100337562 hspa5b, BiP/Grp78; heat shock 70 kDa protein 5b
Length=658
Score = 28.9 bits (63), Expect = 4.0, Method: Composition-based stats.
Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 4/57 (7%)
Query 55 SAETHSLTGKERFTIALNNGKLVPGSIIRVKTDCGKSFET----KCRIDTDVEVEYF 107
+AE K + TI + +L P I R+ TD K E K RIDT E+E +
Sbjct 513 TAEDKGTGNKNKITITNDQNRLTPEEIERMVTDAEKFAEEDKKLKERIDTRNELESY 569
> dre:100332566 protocadherin gamma cluster 1 member-like
Length=820
Score = 28.9 bits (63), Expect = 4.5, Method: Compositional matrix adjust.
Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 0/32 (0%)
Query 60 SLTGKERFTIALNNGKLVPGSIIRVKTDCGKS 91
S +GK+ F++ L NG+L+ +I +T CG+S
Sbjct 68 SESGKQYFSLNLRNGELLVNKVIDRETLCGQS 99
> dre:449546 ric8a, zgc:92294; resistance to inhibitors of cholinesterase
8 homolog A
Length=548
Score = 28.5 bits (62), Expect = 4.8, Method: Compositional matrix adjust.
Identities = 26/90 (28%), Positives = 38/90 (42%), Gaps = 10/90 (11%)
Query 18 GPYLQGVKAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHSLTGKERFTIALNNGKLV 77
G + VK ++ +R+ R N + +LP L ESA H T K L N L
Sbjct 316 GVNMDAVKVLVEFMEKRLDRGNKLKETLLPSLNLLTESARIHRETRK-----FLRNKVLP 370
Query 78 PGSIIRVKTDCGKSFETK-----CRIDTDV 102
P ++ + + G + K IDTDV
Sbjct 371 PLRDVKNRPEVGNALRNKLVRLMTHIDTDV 400
> xla:397850 hspa5, hspa5a; heat shock 70 kDa protein 5a; K09490
heat shock 70kDa protein 5
Length=658
Score = 28.5 bits (62), Expect = 4.9, Method: Compositional matrix adjust.
Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 10/69 (14%)
Query 55 SAETHSLTGKERFTIALNNGKLVPGSIIRVKTDCGKSFET----KCRIDTDVEVEYFRNG 110
+AE K + TI + +L P I R+ TD K E K RIDT E+E +
Sbjct 513 TAEDKGTGNKNKITITNDQNRLTPEEIERMVTDAEKFAEEDKKLKERIDTRNELESY--- 569
Query 111 GALHYVLRN 119
Y L+N
Sbjct 570 ---AYSLKN 575
> xla:379756 hspa5, BiP, MGC52648, grp78; heat shock 70kDa protein
5 (glucose-regulated protein, 78kDa); K09490 heat shock
70kDa protein 5
Length=655
Score = 28.5 bits (62), Expect = 5.0, Method: Compositional matrix adjust.
Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 10/69 (14%)
Query 55 SAETHSLTGKERFTIALNNGKLVPGSIIRVKTDCGKSFET----KCRIDTDVEVEYFRNG 110
+AE K + TI + +L P I R+ TD K E K RIDT E+E +
Sbjct 513 TAEDKGTGNKNKITITNDQNRLTPEEIERMVTDAEKFAEEDKKLKERIDTRNELESY--- 569
Query 111 GALHYVLRN 119
Y L+N
Sbjct 570 ---AYSLKN 575
> dre:30122 wnt8a, wnt-8, wnt8, wu:fa20e02, wu:fe05d07; wingless-type
MMTV integration site family, member 8a; K00714 wingless-type
MMTV integration site family, member 8
Length=359
Score = 28.5 bits (62), Expect = 5.7, Method: Compositional matrix adjust.
Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 1/76 (1%)
Query 25 KAVIAQSFERIHRSNLVGMGILPLQFLKGESAETHSLTGKERFTIALNNGKLVPGSIIRV 84
+ IA +F + R+ L+ M P +K S H G+E N + S R+
Sbjct 235 RGAIADTFSSVARTELIFMEDSPDYCVKNLSMGLHGTEGRECLQSGKNLSQWERRSCRRL 294
Query 85 KTDCGKSFETKCRIDT 100
+CG E + RI+T
Sbjct 295 CHECGLKVEER-RIET 309
> ath:AT5G57160 ATLIG4; ATLIG4; DNA ligase (ATP)/ protein binding;
K10777 DNA ligase 4 [EC:6.5.1.1]
Length=1219
Score = 28.5 bits (62), Expect = 6.2, Method: Compositional matrix adjust.
Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Query 89 GKSFETKCRIDTDVEVEYFRNGGALHYVLRNILN 122
GK +C+ D D ++ +NG +HY RN L+
Sbjct 245 GKDVVAECKFDGD-RIQIHKNGTDIHYFSRNFLD 277
Lambda K H
0.318 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2059772308
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40