bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_6940_orf1
Length=98
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_064450 DNA topoisomerase III beta-1, putative (EC:5... 139 2e-33
mmu:21976 Top3b; topoisomerase (DNA) III beta (EC:5.99.1.2); K... 118 4e-27
xla:398564 top3b, MGC53016; topoisomerase (DNA) III beta (EC:5... 116 1e-26
hsa:8940 TOP3B, FLJ39376; topoisomerase (DNA) III beta (EC:5.9... 115 3e-26
dre:100329289 topoisomerase (DNA) III beta-like; K03165 DNA to... 114 8e-26
cpv:cgd6_2390 DNA topoisomerase III beta-1 ; K03165 DNA topois... 109 2e-24
ath:AT2G32000 DNA topoisomerase family protein; K03165 DNA top... 102 4e-22
bbo:BBOV_II000360 18.m06014; DNA topoisomerase; K03165 DNA top... 96.3 2e-20
cel:Y48C3A.14 hypothetical protein; K03165 DNA topoisomerase I... 62.0 4e-10
mmu:21975 Top3a, MGC106383; topoisomerase (DNA) III alpha (EC:... 40.4 0.001
xla:444125 top3a, MGC80537, xtop3a; topoisomerase (DNA) III al... 39.3 0.003
sce:YLR234W TOP3, EDR1; DNA Topoisomerase III, conserved prote... 35.4 0.048
tgo:TGME49_026480 DNA topoisomerase III alpha, putative (EC:5.... 29.6 2.5
cel:R03G8.1 hypothetical protein 29.6 2.7
mmu:217012 Unc45b, AA445617, Cmya4, D230041A13Rik, MGC91090, U... 29.6 2.7
tgo:TGME49_023590 proteasome component PRE3 precursor, putativ... 28.1 6.3
> tgo:TGME49_064450 DNA topoisomerase III beta-1, putative (EC:5.99.1.2);
K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=841
Score = 139 bits (351), Expect = 2e-33, Method: Compositional matrix adjust.
Identities = 66/97 (68%), Positives = 77/97 (79%), Gaps = 0/97 (0%)
Query 2 LGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVNK 61
LG+CLVHG M ID +LVLP VRASIE LVDVIA+GKA AVV HSL +FK KF YF+ +
Sbjct 522 LGVCLVHGYMQIDPDLVLPSVRASIEALVDVIAQGKADITAVVTHSLYMFKLKFAYFLKQ 581
Query 62 VERMDALFDVTFTALSATGNPLSRCGRCNRYMNFIAR 98
+ERMD LF+ +FT L+ TGN LSRCGRCNRYMN I +
Sbjct 582 IERMDMLFEASFTMLAQTGNALSRCGRCNRYMNLITK 618
> mmu:21976 Top3b; topoisomerase (DNA) III beta (EC:5.99.1.2);
K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=862
Score = 118 bits (296), Expect = 4e-27, Method: Composition-based stats.
Identities = 52/96 (54%), Positives = 74/96 (77%), Gaps = 0/96 (0%)
Query 1 DLGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVN 60
+LGI LVHG IDAELVLP +R+++E+ +++IA+GKA V+ H+L IFK KF YFV+
Sbjct 539 NLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVD 598
Query 61 KVERMDALFDVTFTALSATGNPLSRCGRCNRYMNFI 96
+ MD L +V+F+ L+ATG PLSRCG+C+R+M +I
Sbjct 599 SIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYI 634
> xla:398564 top3b, MGC53016; topoisomerase (DNA) III beta (EC:5.99.1.2);
K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=858
Score = 116 bits (291), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 52/95 (54%), Positives = 74/95 (77%), Gaps = 0/95 (0%)
Query 2 LGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVNK 61
LGI LVHG IDAELVLP +R+++E+ +++IA+GKA + V+ H+L IFK KF YFV+
Sbjct 540 LGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKANFQQVLDHTLDIFKRKFHYFVDS 599
Query 62 VERMDALFDVTFTALSATGNPLSRCGRCNRYMNFI 96
+ MD L +V+F+ L+ATG PLSRCG+C+R+M +I
Sbjct 600 IAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYI 634
> hsa:8940 TOP3B, FLJ39376; topoisomerase (DNA) III beta (EC:5.99.1.2);
K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=862
Score = 115 bits (288), Expect = 3e-26, Method: Compositional matrix adjust.
Identities = 51/96 (53%), Positives = 74/96 (77%), Gaps = 0/96 (0%)
Query 1 DLGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVN 60
+LGI LVHG IDAELVLP +R+++E+ +++IA+GKA V+ H+L +FK KF YFV+
Sbjct 539 NLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVD 598
Query 61 KVERMDALFDVTFTALSATGNPLSRCGRCNRYMNFI 96
+ MD L +V+F+ L+ATG PLSRCG+C+R+M +I
Sbjct 599 SIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYI 634
> dre:100329289 topoisomerase (DNA) III beta-like; K03165 DNA
topoisomerase III [EC:5.99.1.2]
Length=861
Score = 114 bits (285), Expect = 8e-26, Method: Compositional matrix adjust.
Identities = 51/96 (53%), Positives = 74/96 (77%), Gaps = 0/96 (0%)
Query 1 DLGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVN 60
+LGI LVHG IDA+LVLP +R+++E+ + +IA GKA + V+QH+L IFK KF YFV+
Sbjct 539 NLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQHTLDIFKRKFHYFVD 598
Query 61 KVERMDALFDVTFTALSATGNPLSRCGRCNRYMNFI 96
+ MD L +V+F+ ++ATG PLSRCG+C+R+M +I
Sbjct 599 SIAGMDELMEVSFSPIAATGKPLSRCGKCHRFMKYI 634
> cpv:cgd6_2390 DNA topoisomerase III beta-1 ; K03165 DNA topoisomerase
III [EC:5.99.1.2]
Length=835
Score = 109 bits (273), Expect = 2e-24, Method: Composition-based stats.
Identities = 48/96 (50%), Positives = 71/96 (73%), Gaps = 0/96 (0%)
Query 2 LGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVNK 61
LGI LVHG+M ID ELVLP +R+ +E+ VD+IAKGK +AV++HSL +FK KF YF
Sbjct 539 LGIALVHGIMNIDHELVLPKIRSEMEKYVDLIAKGKYNHKAVLKHSLSVFKLKFLYFAEN 598
Query 62 VERMDALFDVTFTALSATGNPLSRCGRCNRYMNFIA 97
+ ++LF + FT ++A+ + +SRCG+C RY+ +I+
Sbjct 599 ISLFESLFRLGFTNITASCSRISRCGQCKRYLTYIS 634
> ath:AT2G32000 DNA topoisomerase family protein; K03165 DNA topoisomerase
III [EC:5.99.1.2]
Length=838
Score = 102 bits (253), Expect = 4e-22, Method: Compositional matrix adjust.
Identities = 46/96 (47%), Positives = 65/96 (67%), Gaps = 0/96 (0%)
Query 2 LGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVNK 61
LGI L+ G ID +L LP +R+ IEQ + ++AKG+A VVQH ++ F+ KF YFV +
Sbjct 507 LGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQ 566
Query 62 VERMDALFDVTFTALSATGNPLSRCGRCNRYMNFIA 97
+E MDALF+ F+ L+ +G LS+CG+C RYM I
Sbjct 567 IEHMDALFEAQFSPLADSGRALSKCGKCLRYMKHIT 602
> bbo:BBOV_II000360 18.m06014; DNA topoisomerase; K03165 DNA topoisomerase
III [EC:5.99.1.2]
Length=878
Score = 96.3 bits (238), Expect = 2e-20, Method: Compositional matrix adjust.
Identities = 43/97 (44%), Positives = 64/97 (65%), Gaps = 0/97 (0%)
Query 2 LGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVNK 61
LG LV G M +D ELVLP VR +IE ++IA GKA E+V+ H ++F+ KF +++ K
Sbjct 564 LGKALVDGYMQVDPELVLPSVRGAIEGYCNLIADGKADCESVIDHVCRMFRMKFEHYITK 623
Query 62 VERMDALFDVTFTALSATGNPLSRCGRCNRYMNFIAR 98
++ ++ F+ FT + G P+SRCG CNRYM ++ R
Sbjct 624 IDSINTFFETQFTPIEDFGVPMSRCGTCNRYMLYLNR 660
> cel:Y48C3A.14 hypothetical protein; K03165 DNA topoisomerase
III [EC:5.99.1.2]
Length=855
Score = 62.0 bits (149), Expect = 4e-10, Method: Compositional matrix adjust.
Identities = 37/104 (35%), Positives = 51/104 (49%), Gaps = 29/104 (27%)
Query 2 LGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKF------ 55
LG CLV G +D ELVLP +RA +E+ ++++A GKA + + F+ KF
Sbjct 540 LGQCLVRGYWKVDPELVLPTMRAELEEQLNLVASGKA------DYLDRNFEKKFLIENFI 593
Query 56 ---CYFVNKVERMDALFDVTFTALSATGNPLSRCGRCNRYMNFI 96
+FV K E TG P SRCG+C RYM +
Sbjct 594 FFKTFFVEKNEN--------------TGKPFSRCGKCRRYMKLV 623
> mmu:21975 Top3a, MGC106383; topoisomerase (DNA) III alpha (EC:5.99.1.2);
K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=1003
Score = 40.4 bits (93), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 28/102 (27%), Positives = 51/102 (50%), Gaps = 10/102 (9%)
Query 2 LGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVNK 61
LG+ LV G ++ E+ P +RA +E + +I +GK + V++ ++ +K F V K
Sbjct 564 LGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAK 623
Query 62 VERMDA-----LFDVTFTA-----LSATGNPLSRCGRCNRYM 93
+++D L + T A A P+ +C +CN+ M
Sbjct 624 AKKLDEALSQYLGERTEMAQQEEIYPAMPEPVRKCPQCNKDM 665
> xla:444125 top3a, MGC80537, xtop3a; topoisomerase (DNA) III
alpha (EC:5.99.1.2); K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=1022
Score = 39.3 bits (90), Expect = 0.003, Method: Compositional matrix adjust.
Identities = 25/103 (24%), Positives = 48/103 (46%), Gaps = 10/103 (9%)
Query 1 DLGICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFVN 60
+LG+ LV G ++ ++ P +RA +E + +I +GK + V++ ++ +K F
Sbjct 568 ELGMGLVEGYDSMGFQMSKPDLRAELESDLKLICEGKKDKFTVLRLQIQKYKQVFVEAAA 627
Query 61 KVERMDALFDVTFTA----------LSATGNPLSRCGRCNRYM 93
K ++D F + T P+ +C +CNR M
Sbjct 628 KATKLDEALSQYFGQAAEAVPQQEIYAETPLPVRKCPQCNRDM 670
> sce:YLR234W TOP3, EDR1; DNA Topoisomerase III, conserved protein
that functions in a complex with Sgs1p and Rmi1p to relax
single-stranded negatively-supercoiled DNA preferentially,
involved in telomere stability and regulation of mitotic recombination
(EC:5.99.1.2); K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=656
Score = 35.4 bits (80), Expect = 0.048, Method: Compositional matrix adjust.
Identities = 21/61 (34%), Positives = 33/61 (54%), Gaps = 3/61 (4%)
Query 2 LGICLVHGLMAIDAE--LVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCYFV 59
LG+ LVHG AI E PF R +EQ + I +G A + VV+ ++ ++ K+ +
Sbjct 580 LGVSLVHGFEAIGLEDSFAKPFQRREMEQDLKKICEGHASKTDVVKDIVEKYR-KYWHKT 638
Query 60 N 60
N
Sbjct 639 N 639
> tgo:TGME49_026480 DNA topoisomerase III alpha, putative (EC:5.99.1.2);
K03165 DNA topoisomerase III [EC:5.99.1.2]
Length=677
Score = 29.6 bits (65), Expect = 2.5, Method: Compositional matrix adjust.
Identities = 25/108 (23%), Positives = 43/108 (39%), Gaps = 14/108 (12%)
Query 1 DLGICLVHGLMAI----DAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFC 56
DLG+ L G + +L LP +RA +E + ++A+G ++ V+ + K F
Sbjct 543 DLGVALYQGFKRLAEVSGVDLSLPDLRARMEADMALVARGAKTKQEVLDLHVSEMKRVFL 602
Query 57 YFVNKVERMDALF---------DVTFTALSATGNPLSRCGRCNRYMNF 95
+ MD V ++ N S CG+C M+
Sbjct 603 LLKSHAAHMDTEMRNRLGGLEDSVGRDGVTLVAN-FSECGKCRAPMDL 649
> cel:R03G8.1 hypothetical protein
Length=663
Score = 29.6 bits (65), Expect = 2.7, Method: Composition-based stats.
Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Query 32 VIAKGKAPREAVVQHSLKIFKAKFCYFVNKVERMDALFDVTFTALSATGNPLSRC 86
VIA+ K P EA + ++KI + V+KVER+D+ S T P+ C
Sbjct 591 VIAQMKKPSEAKFKATVKITEGNI-KIVSKVERVDSYGKTADCIESETHRPICYC 644
> mmu:217012 Unc45b, AA445617, Cmya4, D230041A13Rik, MGC91090,
Unc45; unc-45 homolog B (C. elegans)
Length=929
Score = 29.6 bits (65), Expect = 2.7, Method: Compositional matrix adjust.
Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
Query 3 GICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGK--APREAVVQ 45
G+ + H L++ DAEL V + + +++ V+ K + R AVVQ
Sbjct 866 GLVIAHNLLSADAELARKLVESELLEILTVVGKQEPDEKRAAVVQ 910
> tgo:TGME49_023590 proteasome component PRE3 precursor, putative
(EC:3.4.25.1); K02738 20S proteasome subunit beta 1 [EC:3.4.25.1]
Length=245
Score = 28.1 bits (61), Expect = 6.3, Method: Compositional matrix adjust.
Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 4/54 (7%)
Query 4 ICLVHGLMAIDAELVLPFVRASIEQLVDVIAKGKAPREAVVQHSLKIFKAKFCY 57
IC+ A D + V V+ I+Q + KG+ PR V+ + +F++ CY
Sbjct 86 ICVCRSGSAADTQAVTQIVKLYIQQYAQELPKGEEPR---VEAAANVFQS-LCY 135
Lambda K H
0.330 0.141 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2050913756
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40