bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_8141_orf1
Length=59
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_121290 pre-mRNA splicing factor 18, putative ; K128... 67.0 1e-11
cpv:cgd7_5490 PRP18 (SFM+PRP18 domains) ; K12817 pre-mRNA-spli... 49.3 3e-06
ath:AT1G03140 splicing factor Prp18 family protein; K12817 pre... 43.1 2e-04
xla:734335 prpf18, MGC85069; PRP18 pre-mRNA processing factor ... 39.3 0.003
hsa:8559 PRPF18, FLJ10210, PRP18, hPrp18; PRP18 pre-mRNA proce... 38.5 0.005
mmu:67229 Prpf18, 2810441A10Rik; PRP18 pre-mRNA processing fac... 38.5 0.005
bbo:BBOV_I003610 19.m02042; WD domain, G-beta repeat domain co... 38.1 0.006
dre:431751 prpf18, zgc:91830; PRP18 pre-mRNA processing factor... 37.4 0.013
hsa:9128 PRPF4, HPRP4, HPRP4P, PRP4, Prp4p; PRP4 pre-mRNA proc... 35.4 0.045
mmu:70052 Prpf4, 1600015H11Rik, AI874830, AW047464, MGC117717,... 35.4 0.047
pfa:PFI1115c pre-mRNA splicing factor, putative; K12817 pre-mR... 35.0 0.063
tgo:TGME49_043540 U4/U6 small nuclear ribonucleoprotein, putat... 34.7 0.078
dre:326944 prpf4, mg:ab03a02, zgc:65943; PRP4 pre-mRNA process... 34.7 0.085
dre:100334455 PRP4 pre-mRNA processing factor 4 homolog; K1266... 34.7 0.085
xla:447476 prpf4, MGC81780; PRP4 pre-mRNA processing factor 4 ... 34.3 0.11
cel:C36B1.5 prp-4; yeast PRP (splicing factor) related family ... 33.5 0.16
tpv:TP01_1183 pre-mRNA splicing factor protein; K12817 pre-mRN... 33.5 0.18
cel:F10G7.10 hypothetical protein; K11978 E3 ubiquitin-protein... 33.1 0.24
bbo:BBOV_IV011260 23.m06381; pre-mRNA splicing factor 18; K128... 32.0 0.44
ath:AT1G24570 hypothetical protein 30.8 0.99
ath:AT2G41500 EMB2776; nucleotide binding; K12662 U4/U6 small ... 30.0 1.9
pfa:MAL13P1.385 RNA binding protein, putative 29.3 3.1
ath:AT1G67850 hypothetical protein 29.3 3.5
cel:F32B6.3 hypothetical protein; K12817 pre-mRNA-splicing fac... 28.9 4.7
> tgo:TGME49_121290 pre-mRNA splicing factor 18, putative ; K12817
pre-mRNA-splicing factor 18
Length=373
Score = 67.0 bits (162), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 28/51 (54%), Positives = 40/51 (78%), Gaps = 0/51 (0%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKNVFLSLQ 53
E+F++LR +K+PVTLFGE+ W+RY RLC LE+ +DE+ GQ+N FL+ Q
Sbjct 51 EIFKKLRKMKEPVTLFGESDWQRYTRLCELELLHHEDELMGGQRNAFLNPQ 101
> cpv:cgd7_5490 PRP18 (SFM+PRP18 domains) ; K12817 pre-mRNA-splicing
factor 18
Length=337
Score = 49.3 bits (116), Expect = 3e-06, Method: Compositional matrix adjust.
Identities = 25/49 (51%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQ-LMDDEMPEGQKNVFL 50
E +RLR + +P+TLFGE ERY RL RLE + ++EM GQ+N+FL
Sbjct 99 ETIKRLRLLGEPITLFGEDDNERYNRLRRLEFKGRTNEEMNIGQQNIFL 147
> ath:AT1G03140 splicing factor Prp18 family protein; K12817 pre-mRNA-splicing
factor 18
Length=420
Score = 43.1 bits (100), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 25/51 (49%), Positives = 31/51 (60%), Gaps = 3/51 (5%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLM---DDEMPEGQKNVFL 50
EV RRLR +KQP+TLFGE R RL + + + D +M EGQ N FL
Sbjct 108 EVIRRLRFLKQPMTLFGEDDQSRLDRLKYVLKEGLFEVDSDMTEGQTNDFL 158
> xla:734335 prpf18, MGC85069; PRP18 pre-mRNA processing factor
18 homolog; K12817 pre-mRNA-splicing factor 18
Length=342
Score = 39.3 bits (90), Expect = 0.003, Method: Compositional matrix adjust.
Identities = 20/45 (44%), Positives = 31/45 (68%), Gaps = 2/45 (4%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKN 47
EV RRLR +P+ LFGET +E +QRL ++E+ + E+ +G +N
Sbjct 83 EVIRRLRERGEPIRLFGETDYETFQRLRKIEI--LAPEVNKGLRN 125
> hsa:8559 PRPF18, FLJ10210, PRP18, hPrp18; PRP18 pre-mRNA processing
factor 18 homolog (S. cerevisiae); K12817 pre-mRNA-splicing
factor 18
Length=342
Score = 38.5 bits (88), Expect = 0.005, Method: Compositional matrix adjust.
Identities = 19/45 (42%), Positives = 31/45 (68%), Gaps = 2/45 (4%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKN 47
EV RRLR +P+ LFGET ++ +QRL ++E+ + E+ +G +N
Sbjct 83 EVIRRLRERGEPIRLFGETDYDAFQRLRKIEI--LTPEVNKGLRN 125
> mmu:67229 Prpf18, 2810441A10Rik; PRP18 pre-mRNA processing factor
18 homolog (yeast); K12817 pre-mRNA-splicing factor 18
Length=342
Score = 38.5 bits (88), Expect = 0.005, Method: Compositional matrix adjust.
Identities = 19/45 (42%), Positives = 31/45 (68%), Gaps = 2/45 (4%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKN 47
EV RRLR +P+ LFGET ++ +QRL ++E+ + E+ +G +N
Sbjct 83 EVIRRLRERGEPIRLFGETDYDAFQRLRKIEI--LTPEVNKGLRN 125
> bbo:BBOV_I003610 19.m02042; WD domain, G-beta repeat domain
containing protein; K12662 U4/U6 small nuclear ribonucleoprotein
PRP4
Length=524
Score = 38.1 bits (87), Expect = 0.006, Method: Composition-based stats.
Identities = 21/55 (38%), Positives = 31/55 (56%), Gaps = 0/55 (0%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKNVFLSLQKEAE 57
EV R LRA+ +P+TLFGE +ER +RL ++ + D N +S EA+
Sbjct 41 EVIRILRALIEPITLFGEGKYERRERLKKVLLFKYDRLFHGKDLNTAISTSSEAK 95
> dre:431751 prpf18, zgc:91830; PRP18 pre-mRNA processing factor
18 homolog (yeast); K12817 pre-mRNA-splicing factor 18
Length=342
Score = 37.4 bits (85), Expect = 0.013, Method: Compositional matrix adjust.
Identities = 18/45 (40%), Positives = 31/45 (68%), Gaps = 2/45 (4%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKN 47
EV RRLR +P+ LFGE+ ++ +QRL ++E+ + E+ +G +N
Sbjct 83 EVIRRLRERGEPIRLFGESDYDAFQRLRKIEI--LAPEVNKGLRN 125
> hsa:9128 PRPF4, HPRP4, HPRP4P, PRP4, Prp4p; PRP4 pre-mRNA processing
factor 4 homolog (yeast); K12662 U4/U6 small nuclear
ribonucleoprotein PRP4
Length=522
Score = 35.4 bits (80), Expect = 0.045, Method: Composition-based stats.
Identities = 16/28 (57%), Positives = 21/28 (75%), Gaps = 0/28 (0%)
Query 2 TEVFRRLRAIKQPVTLFGETPWERYQRL 29
+EV LRA+ +P+TLFGE P ER +RL
Sbjct 106 SEVKACLRALGEPITLFGEGPAERRERL 133
> mmu:70052 Prpf4, 1600015H11Rik, AI874830, AW047464, MGC117717,
bN189G18.1; PRP4 pre-mRNA processing factor 4 homolog (yeast);
K12662 U4/U6 small nuclear ribonucleoprotein PRP4
Length=521
Score = 35.4 bits (80), Expect = 0.047, Method: Composition-based stats.
Identities = 16/28 (57%), Positives = 21/28 (75%), Gaps = 0/28 (0%)
Query 2 TEVFRRLRAIKQPVTLFGETPWERYQRL 29
+EV LRA+ +P+TLFGE P ER +RL
Sbjct 105 SEVKACLRALGEPITLFGEGPAERRERL 132
> pfa:PFI1115c pre-mRNA splicing factor, putative; K12817 pre-mRNA-splicing
factor 18
Length=343
Score = 35.0 bits (79), Expect = 0.063, Method: Composition-based stats.
Identities = 17/47 (36%), Positives = 31/47 (65%), Gaps = 2/47 (4%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKNVF 49
++ LR +K+P+ LFGET +RY RL E+++ +E+ ++N+F
Sbjct 90 QIIMLLRQLKEPIRLFGETDLQRYNRL--KELKMNKNELKINEQNIF 134
> tgo:TGME49_043540 U4/U6 small nuclear ribonucleoprotein, putative
(EC:2.6.1.45); K12662 U4/U6 small nuclear ribonucleoprotein
PRP4
Length=711
Score = 34.7 bits (78), Expect = 0.078, Method: Compositional matrix adjust.
Identities = 15/26 (57%), Positives = 19/26 (73%), Gaps = 0/26 (0%)
Query 4 VFRRLRAIKQPVTLFGETPWERYQRL 29
V + LRA+ P+ LFGE P+ER QRL
Sbjct 95 VRKVLRALGHPICLFGEGPFERRQRL 120
> dre:326944 prpf4, mg:ab03a02, zgc:65943; PRP4 pre-mRNA processing
factor 4 homolog (yeast); K12662 U4/U6 small nuclear ribonucleoprotein
PRP4
Length=507
Score = 34.7 bits (78), Expect = 0.085, Method: Composition-based stats.
Identities = 19/44 (43%), Positives = 27/44 (61%), Gaps = 0/44 (0%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQK 46
EV LRA+ +P+TLFGE P ER +RL + L D + + +K
Sbjct 92 EVKACLRALGEPITLFGEGPAERRERLRNVLSVLGPDALKKSKK 135
> dre:100334455 PRP4 pre-mRNA processing factor 4 homolog; K12662
U4/U6 small nuclear ribonucleoprotein PRP4
Length=507
Score = 34.7 bits (78), Expect = 0.085, Method: Composition-based stats.
Identities = 19/44 (43%), Positives = 27/44 (61%), Gaps = 0/44 (0%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQK 46
EV LRA+ +P+TLFGE P ER +RL + L D + + +K
Sbjct 92 EVKACLRALGEPITLFGEGPAERRERLRNVLSVLGPDALKKSKK 135
> xla:447476 prpf4, MGC81780; PRP4 pre-mRNA processing factor
4 homolog; K12662 U4/U6 small nuclear ribonucleoprotein PRP4
Length=483
Score = 34.3 bits (77), Expect = 0.11, Method: Composition-based stats.
Identities = 16/28 (57%), Positives = 21/28 (75%), Gaps = 0/28 (0%)
Query 2 TEVFRRLRAIKQPVTLFGETPWERYQRL 29
+EV LRA+ +P+TLFGE P ER +RL
Sbjct 90 SEVKACLRALGEPITLFGEGPAERRERL 117
> cel:C36B1.5 prp-4; yeast PRP (splicing factor) related family
member (prp-4); K12662 U4/U6 small nuclear ribonucleoprotein
PRP4
Length=496
Score = 33.5 bits (75), Expect = 0.16, Method: Composition-based stats.
Identities = 16/39 (41%), Positives = 25/39 (64%), Gaps = 0/39 (0%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEM 41
+V +LRA+ QP+ LFGE +R +RL L + +DE+
Sbjct 80 QVKLKLRALNQPICLFGEDALDRRERLRALLSTMSEDEI 118
> tpv:TP01_1183 pre-mRNA splicing factor protein; K12817 pre-mRNA-splicing
factor 18
Length=327
Score = 33.5 bits (75), Expect = 0.18, Method: Composition-based stats.
Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 0/38 (0%)
Query 3 EVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDE 40
EV +RLR ++QP+ FGE+ ER +RL E + D E
Sbjct 84 EVVKRLRKLRQPIVFFGESHKERCRRLFSQETDIDDLE 121
> cel:F10G7.10 hypothetical protein; K11978 E3 ubiquitin-protein
ligase UBR3 [EC:6.3.2.19]
Length=1884
Score = 33.1 bits (74), Expect = 0.24, Method: Compositional matrix adjust.
Identities = 18/39 (46%), Positives = 26/39 (66%), Gaps = 0/39 (0%)
Query 20 ETPWERYQRLCRLEVQLMDDEMPEGQKNVFLSLQKEAEE 58
+T +ERYQRL R + LM+D M +KN+F +KE +E
Sbjct 795 KTEFERYQRLVREQALLMEDMMLTPEKNLFQFDEKETDE 833
> bbo:BBOV_IV011260 23.m06381; pre-mRNA splicing factor 18; K12817
pre-mRNA-splicing factor 18
Length=285
Score = 32.0 bits (71), Expect = 0.44, Method: Compositional matrix adjust.
Identities = 15/49 (30%), Positives = 31/49 (63%), Gaps = 3/49 (6%)
Query 2 TEVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKNVFL 50
+E+ +LR K+P T+F ET +R +RL + + + + + + Q+N+F+
Sbjct 90 SEIINQLRRHKEPATIFAETREDRIERLYQAQEK---ENIAKNQQNIFV 135
> ath:AT1G24570 hypothetical protein
Length=381
Score = 30.8 bits (68), Expect = 0.99, Method: Composition-based stats.
Identities = 11/38 (28%), Positives = 24/38 (63%), Gaps = 0/38 (0%)
Query 19 GETPWERYQRLCRLEVQLMDDEMPEGQKNVFLSLQKEA 56
G+ PW+ + C++E ++ ++ + +K+ F SLQ E+
Sbjct 336 GKAPWQGVRDRCQMEWKMFENRVEAAEKDYFRSLQVES 373
> ath:AT2G41500 EMB2776; nucleotide binding; K12662 U4/U6 small
nuclear ribonucleoprotein PRP4
Length=554
Score = 30.0 bits (66), Expect = 1.9, Method: Compositional matrix adjust.
Identities = 14/26 (53%), Positives = 18/26 (69%), Gaps = 0/26 (0%)
Query 7 RLRAIKQPVTLFGETPWERYQRLCRL 32
RLR + +P+TLFGE ER RL +L
Sbjct 129 RLRRLGEPITLFGEQEMERRARLTQL 154
> pfa:MAL13P1.385 RNA binding protein, putative
Length=648
Score = 29.3 bits (64), Expect = 3.1, Method: Composition-based stats.
Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 10/52 (19%)
Query 8 LRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKNVFLSLQKEAEEE 59
LR +K+P+ LFGE +++ +RL + + D L ++K+ EEE
Sbjct 62 LRLLKEPICLFGEDSYDKRKRLKNILITKYDR----------LIIKKKIEEE 103
> ath:AT1G67850 hypothetical protein
Length=404
Score = 29.3 bits (64), Expect = 3.5, Method: Composition-based stats.
Identities = 12/38 (31%), Positives = 19/38 (50%), Gaps = 0/38 (0%)
Query 19 GETPWERYQRLCRLEVQLMDDEMPEGQKNVFLSLQKEA 56
G+ PW+ + C+ E + M +K+ F SLQ E
Sbjct 358 GKAPWQGVRDRCKKEWTMFQSRMANAEKDYFKSLQVEG 395
> cel:F32B6.3 hypothetical protein; K12817 pre-mRNA-splicing factor
18
Length=352
Score = 28.9 bits (63), Expect = 4.7, Method: Composition-based stats.
Identities = 18/46 (39%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Query 2 TEVFRRLRAIKQPVTLFGETPWERYQRLCRLEVQLMDDEMPEGQKN 47
+E+ RLR P+ LFGET + +RL +LE L ++ EG +N
Sbjct 88 SEIQTRLRQRNHPIMLFGETDIDVRKRLHQLE--LAQPDLNEGWEN 131
Lambda K H
0.318 0.134 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2073802932
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40