NON-INTERFERENCE OF INOVOCOXTM, A LIVE COCCIDIAL OOCYST VACCINE, WITH MAREK’S DISEASE VACCINE OR BURSAPLEX®, A LIVE BURSAL DISEASE VACCINE, AFTER IN OVO ADMINISTRATION

V. W. Doelling, R. M. Poston, A. Martin, L. M. Charniga, L. K. Griffin. D. B. Link and A. P. Avakian
Embrex, Inc., P.O. Box 13989, Research Triangle Park, NC 27709
vdoelling@embrex.com

Studies were conducted to determine the compatibility of Inovocox™, a live coccidiosis vaccine, with other in ovo vaccines. Safety and efficacy of Inovocox and a commercial live Marek’s Disease (MD) HVT-SB1 vaccine or Bursaplex®, a commercial live infectious bursal disease (IBD) vaccine, was determined after co-administration in ovo. Inovocox consists of four strains of coccidia including Eimeria, E. tenella, and two strains of E. maxima. For each study, in ovo treatments included sham-vaccination with diluent, Inovocox alone, MD or IBD vaccine alone, or Inovocox combined with MD or IBD vaccine. Each vaccine preparation was administered in 100 ul of Marek’s diluent at 18 days of embryonation to broiler or SPF eggs. To test Inovocox efficacy, broilers were placed in floor pens and challenged with mixed Eimeria strains at 21 days of age. Efficacy was assessed by weight gain and intestinal lesion scores at six days post challenge. Viral vaccine efficacy was tested in SPF birds in isolation units. Groups receiving MD or IBD viral challenge were monitored for challenge-related mortality. Birds were also necropsied for relevant gross lesions at 49 days post challenge for MD and 3 days post challenge for IBD. There were no differences in percent hatch and no treatment related mortalities in any treatment group. Weight gain and lesion scores in the Inovocox vaccinated treatment groups demonstrated significant protection when Inovocox was administered alone or in combination with MD vaccine or Bursaplex. Both the MD and Bursaplex vaccines demonstrated significant protection when administered with Inovocox or alone. Together, these results demonstrate that co-administration of Inovocox with either MDV or Bursaplex is safe and does not interfere with vaccine efficacy.

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